TNF-α下调bcl-2mRNA表达与HL-60细胞凋亡的关系

以不同浓度TNF-α刺激HL-60细胞,采用DNA凝胶电泳、流式细胞术、RT-PCR等技术,于6h,12h,24h,48h,72h对细胞进行凋亡鉴定.发现一定剂量的TNF-α可诱导HL-60细胞凋亡,HL-60细胞bcl-2mRNA表达明显降低,且与药物呈时间、剂量上的依赖性;IL-2的同时应用,可减轻TNF-α诱导的DNA降解,但bcl-2mRNA的表达无明显改变.结果提示:在粒细胞发生过程中,一定量的TNF-α可直接诱导其产生凋亡,凋亡信号的传导与抑凋基因bcl-2基因的表达有密切关系.     

神经特异性转录因子DAT1 mRNA在大鼠中枢神经系统的定位

目的 观察神经特异性转录因子(DAT1)在大鼠中枢神经系统中的定位。方法 原位杂交组织化学染色。结果 在大鼠中枢神经系统中,DAT1 mRNA阳性反应产物主要位于胞质和突起内,DAT1 mRNA阳性神经元可见于大脑、小脑、丘脑、脑干、脊髓。强阳性信号主要出现于嗅球、梨状皮质、纹状皮质、内嗅皮质、海马CA1区、齿状回、丘脑后外侧核、红核、被盖背侧核、延髓中央网状核、三叉神经运动核、舌下神经核、迷走神经核、楔束外核、疑核等部位;中等强度着色主要分布于大脑皮质Ⅱ～Ⅵ层、海马CA2区和CA3区、杏仁核、苍白球、内侧膝状体、外侧膝状体、视上核、未定带、弓状核、室旁核、黑质、中脑网状核、脑桥网状核、巨细胞网状核、外侧网状核、斜方体内侧核、前庭神经核、蜗神经背核、楔束核、中缝背核;在隔核、乳头体前核、上丘浅灰质层、下丘中央核、下丘外侧核、中央灰质、三叉神经中脑核、三叉脊束核、孤束核、下橄榄核、中央上核、小脑顶核、小脑间置核、小脑外侧核、脊髓灰质及丘脑的大部分核团可见弱的阳性细胞。结论 DAT1广泛分布于大鼠中枢神经系统内,其分布与多巴胺能神经分布密切相关,提示该基因对大鼠中枢神经系统活动,尤其是多巴胺能神经递质活动具有重要的调节功能。     

肺癌细胞bcl-2的表达与凋亡的关系

目的:为研究bcl-2的表达与肺癌细胞凋亡发生的关系.方法:采用免疫组化及末端转移酶介导的切口末端标记法(TUNEL).分别对73例肺癌组织进行bcl-2免疫组化染色和细胞凋亡的检测.并对bcl-2表达强度和细胞凋亡指数(Al)进行相关分析,结果:(1)bcl-2在肺癌组织中总的阳性率为27,4%,其中小细胞癌的阳性率为54%;鳞癌阳性率23%;腺癌阳性率18.7%(2)肺癌细胞AI为0到17.6%,其中有13例AI<1%,29例AI在1%到5%之间,31例AI>5%.(3)bcl-2表达的强应与肺癌细胞凋亡指数呈显著负相关.r=0.6216,P<0.01.结论:肺癌组织中bcl-2的过表达可能与抑制细胞凋亡有关.     

人胎儿中枢神经系统星形胶质细胞形态发育的观察

为观察人胎儿中枢神经系统星形胶质细胞形态发育。用胶质原纤维酸性蛋白抗体进行免疫组织化学染色。结果表明；１．以颈段脊髓，脑干，海马和小脑蚓部于胚胎２５周其ＧＦＡＰ染色强度，细胞密度接近出生时水平。而此时期大脑皮层Ａｓｔ密度约为出生时的四分之一。２．在同一胎龄ＣＮＳ的不同部位，ＧＦＡＰ阳性Ａｓｔ分布不均匀。３．Ａｓｔ不仅在毛细胞血管周围，而且在小血管周围密度大染色深，环绕血管呈辐射状排列。     

凋亡抑制因子5在卵巢癌中的表达及临床意义

目的检测凋亡抑制因子5(API5)在卵巢癌中的表达,分析其与临床病理特征的关系。方法Western blotting方法检测2例正常卵巢组织及6例不同级别的卵巢癌组织的表达,组织化学方法检测10例正常卵巢、119例卵巢癌(浆液性腺癌61例、黏液性癌5例、内膜样癌10例、透明细胞癌10例及未分化癌33例)组织中API5的表达,分析其表达与临床病理参数及5年生存率之间的相关性,细胞转染及凋亡流式细胞分析检测API5及顺铂对卵巢癌细胞的作用。结果 API5在卵巢癌组织中的表达高于正常卵巢组织,API5的表达与临床病例特征之间密切相关,Kaplan-Meier生存曲线揭示API5表达高的卵巢癌患者较低表达患者生存率低,流式细胞术分析显示,抑制API5的表达能增加顺铂对卵巢癌细胞的敏感性。结论 API5的异常表达与卵巢癌的发生密切相关,提示API5在预后评价中具有潜在的临床应用价值,有可能成为卵巢癌治疗的潜在靶点。     

尘粒引起人支气管肺淋巴结巨噬细胞的凋亡和bcl-2表达

目的 研究人支气管肺淋巴结尘细胞和大鼠腹膜腔巨噬细胞吞噬碳粒后的凋亡和bcl-2表达,探讨巨噬细胞凋亡与淋巴结结构变化之间的关系。方法 取人支气管肺淋巴结,作石蜡切片和超薄切片,观察尘粒分布、凋亡尘细胞和组织结构变化。用TUNEL染色法和bcl-2抗体标记法观察淋巴结内尘细胞和碳粒处理后的巨噬细胞的凋亡变化和bcl-2表达。结果成人淋巴结巨噬细胞的尘粒明显沉积,淋巴组织减少,胶原纤维增生,血管密度增加。在超薄切片上,尘细胞的细胞核固缩,出现空泡。淋巴结尘细胞和巨噬细胞吞噬碳粒后24h都出现TUNEL染色和bcl-2抗体标记阳性细胞。分解尘粒和碳粒活跃的巨噬细胞,bcl-2表达特别明显。结论 尘粒沉积引起人支气管肺淋巴结巨噬细胞的凋亡和凋亡抑制基因bcl-2高表达,尘细胞凋亡与成人支气管肺淋巴结的结构变化有关。     

肝胆管癌中bcl-2、bax基因表达和细胞凋亡的研究

目的　探索细胞凋亡及相关基因在体内肝胆管癌发生中所起的作用。　方法　用mRNA原位杂交技术和免疫组织化学方法 ,检测了肝胆管癌中bcl 2和bax基因表达 ;利用TUNEL法检测其细胞凋亡 ;　结果　肿瘤中bcl 2表达增强 ,癌旁组织中bax表达增强 ,癌旁组织中凋亡细胞增加 ;　结论　bcl 2基因激活后 ,抑制bax的作用及基因表达 ,可能使细胞的增殖加快 ,肿瘤发生 ;而bax的表达可能引起癌旁组织的细胞凋亡加剧     

bcl-2家族促凋亡成员--bak与肿瘤

bcl-2基因家族是一类细胞凋亡的相关基因,参与细胞凋亡的调控.bak作为该家族的成员,虽发现较晚,但因其显著的促凋亡作用,现在已受到越来越多的关注.本文就bak基因及其蛋白产物BAK与肿瘤之间的关系做一综述.     

人胎儿晶状体上皮细胞增殖、凋亡及相关因子的研究

本实验应用TUNEL和免疫组化法，选用人正常胎儿晶状体，分为9，10，12，16，20，24，28，32周，每组6例，观察了晶状体发育过程中，晶状体上皮细胞凋亡率以及PCNA、EGF、TGF-β1的变化，以期为基础医学和临床医学对晶状体研究提供理论依据和形态学资料。PCNA、EGF、TGF-β1在人胎儿不同胎龄晶状体上皮细胞均有表达，     

抗凋亡基因bcl-2在大肠癌中的表达及意义

本研究采用免疫组织化学ＳＡＢＣ法对７７例大肠癌ｂｃｌ－２进行了检测。结果表明，在７７例大肠癌中，ｂｃｌ－２阳性６７例（８７．０１％）；在作为对照的５７例癌周组织中，ｂｃｌ－２阳性占４７例（８２．４５％）；在４６例高分化的大肠癌中，比１５例低分化腺癌表达要强（Ｐ＜０．０１）。结论：ｂｃｌ－２在绝大部分大肠癌中都有表达，这表明其参与了大肠癌的凋亡调控，其表达与大肠癌的分化有关。     

Selective central nervous system tropism of primary central nervous system lymphoma

Primary Central nervous system lymphoma (PCNSL) is most frequently a diffuse large B cell lymphoma (DLBCL), which is confined to the Central nervous system (CNS). We performed an experiment in which lymphoma cells from a PCNSL patient were implanted subcutaneously in an athymic mouse. The lymphoma cells were shown to home to the CNS with histologic evaluations of the brain showing multiple large B cells in blood vessels consistent with intravascular large B cell lymphoma (IVL). We did not find any evidence of lymphoma at the site of implantation or other locations. The findings are consistent with highly selective tropism of PCNSLforthe CNS and its vasculature.     

原发性中枢神经系统淋巴瘤bcl-2蛋白和CD56的表达

目的：探讨原发性中枢神经系统淋巴瘤中bcl-2蛋白的表达情况及bcl-2蛋白阳性表达与CD56表达的相关性。方法：应用免疫组织化学S－P方法对21例原发性中枢神经系统淋巴瘤组织中bcl-2蛋白和CD56进行检测。结果：21例原发性中枢神经系统淋巴瘤中17例bcl-2蛋白表达阳性（81．0％）,阳性瘤细胞多呈弥漫分布,以中心细胞样细胞表达为主。21例原发性中枢神经系统淋巴瘤中bcl-2蛋白有较高的表达率,提示由bcl-2基因产物介导的细胞凋亡障碍与中枢神经系统淋巴瘤的发生有关;而原发性中枢神经系统淋巴瘤亦有CD56的表达,其可能与肿瘤侵袭性有关。     

Apoptosis and Bcl-2 oncoprotein expression in the human fetal central nervous system

Apoptosis and the apoptosis-regulatory gene bcl-2 have been suggested from animal studies to be important during the development of the central nervous system (CNS), but information on apoptotic activities of the developing human CNS has been scarce. To establish spatial and temporal distributions of apoptotic cells and Bcl-2 oncoprotein expression, we examined sections taken from cerebral cortex, hippocampus and brainstem at weeks 14, 18, 27, and 32 of gestation. Terminal transferase-mediated nick end labelling (TUNEL), histological analyses, and immunocytochemical staining using monoclonal antibodies were employed. Except for layer I of the motor cortex and the molecular layer of the hippocampus, both at week 14 of gestation, TUNEL-positive cells with typical apoptotic appearance and apoptotic indices, ranging from 0.08 to 2.85, were found in all other brain regions examined including visual, sensory, frontal and motor cortices, hippocampus, dorsal raphé, locus coeruleus, and periaqueductal grey of the brainstem. No specific spatial or temporal distribution patterns of apoptotic cells were found in the cortices. However, the apoptotic index of the molecular layer of the hippocampus increased with the gestation age. The periaqueductal grey of the brainstem showed high apoptotic indices (ranging from 0.37 to 2.85) at all the gestation ages studied. An inverse correlation between apoptosis and Bcl-2 oncoprotein expression was found in visual, sensory, and motor cortices but not in the frontal cortex and hippocampus. Apoptosis and Bcl-2 oncoproteins are important for CNS development and, apart from being an apoptosis regulator, Bcl-2 oncoproteins may also have other roles to play during neural development. Anat. Rec. 252:165–175, 1998. © 1998 Wiley-Liss, Inc.     

极性蛋白与中枢神经系统发育

哺乳动物大脑神经元的形态多样性和突触连接的复杂性是极性细胞的典型例子,形成和维持神经元极性依赖多种极性蛋白的调节.从线虫受精卵发育到哺乳动物神经细胞的极性化通路中,许多极性蛋白存在进化保守机制.中枢神经系统发育的整个过程(包括神经元发生与移行、神经突生长以及突触联系的形成等)都有极性蛋白的直接或间接参与,是各种极性蛋白...     

清络通痹颗粒对胶原性关节炎大鼠滑膜细胞凋亡相关基因p53和bcl-2表达的影响

目的：观察清络通痹颗粒对胶原性关节炎大鼠滑膜凋亡相关基因的影响。方法：采用牛Ⅱ型胶原制备大鼠胶原性关节炎模型，提取滑膜细胞总RNA，采用RT—PCR技术，检测药物对滑膜细胞p53 mRNA、bcl-2 mRNA的影响。结果：胶原性关节炎大鼠滑膜细胞ps3mRNA、bcl-2mRNA表达较正常组明显升高（P〈0．01），清络通痹颗粒7．2g／kg可明显下调胶原性关节炎大鼠滑膜细胞p53 mRNA、bcl-2mRNA的水平（P〈0．05，P〈0．01）。结论：清络通痹颗粒可能通过下调滑膜细胞凋亡相关基因p53 mRNA、bcl-2 mRNA表达，促进滑膜细胞的凋亡而发挥治疗作用。     

Nestin in central nervous system cells

This literature review reflects current knowledge on the intermediate filament protein nestin, which most authors regard as a marker of “neural stem/progenitor cells.” The structural-functional characteristics of nestin and its presence in various central nervous system cells at different stages of ontogenesis in normal and pathological conditions are discussed. __________ Translated from Morfologiya, Vol. 131, No. 1, pp. 85–90, January–February, 2007. Director: Corresponding Member of the Russian Academy of Medical Sciences Professor V. A. Otellin     

Prevalence of human parechoviruses in central nervous system infections in children: A retrospective study in Shanghai,China

Human parechoviruses (HPeV) are associated with central nervous system (CNS) infections and sepsis‐like illnesses. However, data from China are not available. The aim of this study was to determine the prevalence, age, and seasonal distributions and genotypes of HPeV infections in children with CNS related disease in Shanghai, China. Of 776 cerebrospinal fluid (CSF) samples collected from children with CNS‐related diseases under the age of 16 years during the years 2008–2011, 68 (9%) were identified to be HPeV positive. The annual prevalence varied remarkably: 1% (2/153) in 2008, 7% (12/177) in 2009, 15% (23/153) in 2010, and 11% (31/293) in 2011. The virus was detected in all age groups of children ranging from 2 days to 13 years and the median age was 14 months. Of the 31 positive samples that were genotyped successfully, 28 were HPeV1 and 3 were HPeV3. This study provided data on the molecular epidemiology of HPeV infections in CNS‐related diseases in Shanghai, China and suggest that the screening for HPeV by PCR should be included in the routine viral testing of CSF. J. Med. Virol. 85:320–326, 2013. © 2012 Wiley Periodicals, Inc.     

氨茶碱对哮喘豚鼠嗜酸细胞凋亡及bcl—2 mRNA表达的影响

目的 观察氨茶碱对体外不同密度嗜酸细胞（Eos）凋亡及bcl-2 mRNA表达的影响,探讨其促进哮喘Eos凋亡的机制。方法 卵蛋白激发哮喘豚鼠动物模型48h后行支气管肺泡灌洗,分离不同密度Eos。氨茶碱（AM）（10^-6-10^-3mol/L)干预24h,原位杂交检测Eos的bcl-2 mRNA表达,TUNL法检测细胞凋亡。结果 AM干预24h,可见Eos凋亡增加,以低密度Eos(HEos)为明显,bcl-2 mRNA表达,呈剂量依赖性。bcl-2 mRNA的表达与Eos凋亡呈负相关。结论 AM可抑制bcl-2 mRNA表达,促进Eos凋亡。bcl-2参与了AM对Eos凋亡的调节。