针刺复合全麻对胆囊切除术患者围手术期血流动力学的影响

目的 观察针刺复合全身麻醉对腹腔镜胆囊切除术患者围术期血流动力学的影响。方法 选择择期腹腔镜胆囊切除手术ASAⅠ－Ⅱ级患者60例,随机分成两组,分别用全麻（对照组）和针刺复合全麻（针麻组）。两组均以咪达唑仑、芬太尼、丙泊酚、维库溴铵诱导,针麻组在全麻诱导前应用针麻仪刺激器（取双侧内关、合谷、曲池穴）进行电针刺激,针麻诱导15 min后与全麻组一样开始全麻诱导和维持。并运用胸电生物阻抗法无创连续血流动力监测仪监测麻醉诱导前基础值、麻醉诱导后意识消失时、气管插管即刻、手术开始时、术毕、拔管时、拔管后10 min的心功能和血流动力学变化。结果 两组患者手术持续时间和麻醉维持时间均无统计学意义。单位时间维库溴铵用药量针麻组比对照组明显减少（P＜0.05）。对照组平均动脉压（MAP）、心率（HR）、心率收缩压乘积（RPP）、心排血量（CO）、外周血管阻力（SVR）及血流加速度指数（ACI）在麻醉诱导期均显著低于麻醉诱导前（P＜0.05或P＜0.01）, 在插管即刻和拔管时均显著高于麻醉诱导前（P＜0.05或P＜0.01）,而针麻组无明显变化;插管即刻和拔管时,MAP、RPP、SVR和ACI对照组明显高于针麻组（P＜0.05或P＜0.01）。结论 针刺复合麻醉具有双向调节作用,使术中血流动力学波动小,血液循环趋于稳定,并且能减少麻醉药用量,具有广泛的应用价值。     

Clinical effect of additional electroacupuncture on thoracolumbar intervertebral disc herniation in 80 paraplegic dogs

The clinical efficacy of electroacupuncture and acupuncture in combination with medication for the treatment of thoracolumbar intervertebral disc herniation was investigated in paraplegic dogs with intact deep pain perception. To evaluate the additional effect of electroacupuncture, dogs treated with conventional medicines alone were compared to dogs treated with electroacupuncture and acupuncture and conventional medicine. Medical records of 80 dogs were reviewed for this investigation and classified into two groups undergoing different treatment methods: (1) treatment with conventional medicine alone (Group C, n = 37) and (2) treatment with conventional medicine combined with electroacupuncture and acupuncture (Group CE, n = 43). Prednisone was the conventional medicine and electroacupuncture was applied at GV07 and GV02-1 at 0.5-2.5 mV, mixed Hz of 2 and 15 Hz for 25-30 min. Acupuncture was performed locally at urinary bladder meridian points near the lesion, and bilaterally distantly at GB30, GB34, and ST36. Treatment efficacy was evaluated by post-operative neurologic function, ambulation, relapse, complication, and urinary function. Ambulation recovery was more prevalent in Group CE than Group C (p = 0.01) and recovery of ambulation and back pain relief time was shorter in Group CE compared to Group C (p = 0.011 and 0.001, respectively). Relapse rate was significantly lower in Group CE (p = 0.031). The results suggest that a combination of electroacupuncture and acupuncture with conventional medicine is more effective than conventional medicine alone in recovering ambulation, relieving back pain, and decreasing relapse. Electroacupuncture and acupuncture is thus a reasonable option for the treatment of intervertebral disc herniation in paraplegic dogs with intact deep pain perception.     

The effect of acupuncture at PC-6 on the electroencephalogram and electrocardiogram

The present study aims to examine the effect of acupuncture stimulation of an acupuncture point (PC-6) and nonacupuncture point on electroencephalograms (EEGs) and electrocardiograms (ECGs). We used EEG in 10 healthy subjects to investigate cortical activation during stimulation of acupuncture points (neiguan: PC-6) and nonacupuncture points. Our most interesting finding was the marked differences of amplitude of EEG power between acupuncture points and nonacupuncture points stimulation. Wavelet transform was used as the EEG signal processing method, because it has advantages in a time domain and frequency domain characteristics analysis. EEGs were collected from 16 channels, and the alpha-wave (8-13 Hz), beta-wave (13-30 Hz), theta-wave (4-8 Hz) and delta-wave (0.5-4 Hz) were used as standards for frequency bands. According to the experiment results, EEG signals increased considerably after acupuncture stimulation; in each frequency band, the average amplitude was higher after acupuncture stimulation; ECG heart rates were faster by at least 10% after acupuncture stimulation. Consequently, it will be possible to verify the function of acupuncture stimulation on neiguan (acupuncture points; PC-6) more effectively.     

Effects of acupuncture on nNOS and iNOS expression in the rostral ventrolateral medulla of stress-induced hypertensive rats

This study was to observe the changes of the neuronal and inducible nitric oxide synthase (nNOS & iNOS) as well as their mRNAs in the rostral ventrolateral medulla (RVLM) of stress-induced hypertensive rats before and after acupuncture, and thereby to infer the curative mechanism of acupuncture on hypertension. The result indicated that the systolic blood pressure (SBP) of stress group rats was increased significantly (P < 0.01), it was accompanied that the expression of nNOS in the RVLM, including the immunoreactive neuron number (P < 0.05), the optical density (OD) (P < 0.01), and the mRNA (P < 0.01) were obviously elevated, while those of iNOS (P < 0.05, P < 0.01 and P < 0.01) were evidently lowered in the stress-induced hypertensive rats. Electroacupuncture (EA) points at "Zusanli" (St. 36) and "Lanwei" (Extra 37) on the same hindlimb were stimulated by an EA apparatus (Type G6805-2) with dense sparse wave (4-20Hz) and 4mA intensity. EA application could return the SBP (P < 0.05), and the changes on the expression of both nNOS and iNOS (P < 0.05, P < 0.01 and P < 0.01). These results suggest that the curative mechanism of acupuncture on stress-induced hypertension is related to the changes of nNOS and iNOS in the RVLM of rats.     

快速老化痴呆小鼠海马、皮层p130表达的增龄变化及针刺的影响

目的:观察针刺对快速老化痴呆小鼠(SAMP10,简称P10)脑中p130表达的影响,探讨针刺治疗痴呆的可能机制。方法:随机将P10分为对照组、针刺组、非穴组,同源正常老化小鼠(SAMR1,简称R1)为正常对照。针刺组采用"益气调血,扶本培元"针法(简称"气血"针法),选穴"膻中"中脘"气海"、双侧"足三里"、双侧"血海"。"血海"用捻转泻法,余穴施捻转补法,每穴30s,每天1次,第7天暂停,连续13d。运用western blotting技术,观察2、4、6、8、10、12月龄P10海马和皮层中p130表达的增龄变化,以及"气血"针法对8月龄P10海马和皮层p130表达的影响。结果:①p130表达在正常老化R1海马和皮层均随增龄呈平稳上升趋势,6月龄后与2月龄存在明显差异(P<0.05,0.01)。P10则呈波动下降趋势,海马中4月龄后表达即下降,6月龄达谷值(P<0.01),6至8月龄回升,8月龄低于2月龄水平(P<0.05),8至12月龄又下降;皮层中2至4月龄下降,4月龄后开始上升,至6月龄达峰值(P<0.01),6至8月龄下降至低谷,8至12月龄又上升,但仍停留在较低水平。②与8月龄R1相比,p130在P10海马和皮层表达分别呈上调与下调。"气血"针法具有腧穴特异性,可良性调节皮层的蛋白表达,使之接近R1水平。结论:p130表达在R1与P10同脑区间随增龄存在差异,且p130的波动期与报道的P10生物学特征出现的时间段接近。"气血"针法可良性调节8月龄P10皮层p130的表达。     

Clinical observation on acupuncture treatment of functional dyspepsia

目的:观察针刺治疗功能性消化不良的临床疗效.方法:根据中医经络理论选择以足三里等穴位,用平补平泻手法,辅以电针治疗功能性消化不良,并以常用西药多潘立酮作为对照进行临床观察.结果与结论:针刺组总有效率93.8%,显效率为63.3%,药物组分别为82.3%和43.1%,针刺组治疗非特异型功能性消化不良疗效好于药物组(P＜0.05);治疗动力障碍型者,两者无明显差异(P＞0.05),疗效均较突出.     

电针夹脊穴对海洛因依赖者心理渴求及血浆β-EP、Dyn-A的影响*

目的：研究电针夹脊穴干预海洛因依赖者心理渴求的临床效应及对血浆β-EP、Dyn的影响。 方法：将120例海洛因依赖者随机分为4组：针刺1组（夹脊穴、肾俞）、针刺2组（四肢穴）、模拟组和对照组,采用心理渴求程度VAS量表评定心理渴求程度变化,采用放射免疫法检测血浆β-EP、Dyn治疗前后的变化。 结果：针刺1组、针刺2组心理渴求程度VAS积分明显低于对照组和模拟组(p<0.05, p<0.01),针刺1组与针刺2组之间具有统计学差异(p<0.05),针刺1组、针刺2组血浆β-EP、Dyn水平均有所上升,与对照组和模拟组相比均有显著性差异（p<0.01、p<0.05）。针刺1组和针刺2组的β-EP之间无显著性差异（p>0.05）,针刺1组和针刺2组的Dyn水平相比具有显著性差异（p<0.05）。 结论：电针夹脊穴可明显抑制心理渴求,降低复吸率。其机制可能与增加血浆β-EP的含量,尤其是促进血浆Dyn的增加有关。     

皮层体感Ⅱ区对丘脑腹后外侧核针刺效应的下行调制

<正> 近十余年,越来越多的证据表明丘脑腹侧基底核(VB)内确实含有感受伤害性信息的神经元。研究又证实大脑皮层不仅可感受痛信息,还参与痛调制,大脑皮层的下行调制作用参与针刺镇痛过程。在丘     

Hypothalamic effects of millimeter wave irradiation depend on location of exposed acupuncture zones in unanesthetized rabbits

On nine unanesthetized male rabbits, the frequency spectra of hypothalamic electrogram (EEG) were studied during low intensity (10 mW/cm2) millimeter wave (55-75 GHz) exposure to various acupuncture points (zone): auricular, cranial and corporal. The chances of occurrence of significant (p < 0.05) changes in the EEG spectra during irradiation versus, sham experiments were equal to 31, 21 and 5%, respectively. Exposure to auricular zone reduced the EEG power in narrow bands with central frequencies of 5.3, 15.9 Hz and increased ones of 2.6, 3.2, 6.9, 7.9, 11.5 and 25.6 Hz. The main effect of exposure to cranial zone was similar--changes at 15.9 and 25.6 Hz only. The data obtained demonstrate that the responsiveness of the central nervous system to low intensity millimeter wave radiation may depend on the location of the exposed acupuncture zone.     

Acupuncture for treating temporomandibular disorder: retrospective study on safety and efficacy

This study aimed to retrospectively examine the efficacy and safety of acupuncture for the relief of pain originating from temporomandibular joint disorder and trigeminal neuralgia. Participants included patients suffering from trigeminal neuralgia or temporomandibular disorder with osseous pathology ruled out by panoramic X-rays. Participants received a series of 8-10 weekly acupuncture treatments and rated their pain via a visual analogue scale. From assessment of a total of 39 patients, analysis of pain severity before and after treatment showed that acupuncture intervention was highly beneficial for patients with temporomandibular joint disorder (88.6%, p < 0.01), compared with patients with trigeminal neuralgia in which there was only a minor effect (25%). The data also demonstrated that acupuncture was both efficacious in acute patients (91%, p < 0.01) and chronic patients (70%, p < 0.05) and elicited no side effects during the course of treatment. Acupuncture treatment was a safe and efficient methodology for relieving the pain of patients suffering from temporomandibular disorder with no detectable osseous joint component. Based on these results, a randomized clinical trial is being initiated at the Stomatologic Clinic at the Tel Aviv Sourasky Medical Center to assess the role of acupuncture in treating temporomandibular joint disorder.     

Action mechanism of Yi Guan Jian Decoction on CCl4 induced cirrhosis in rats

Aim of study

To investigate action mechanism of Yi Guan Jian Decoction on cirrhosis induced by CCl₄ in rats.

Material and methods

CCl₄ (3 mL/kg) for the first time and then olive oil CCl₄ solution 50% (2 mL/kg) was administered hypodermically to rats twice each week for 12 weeks. At the end of 8th week, rats were randomly divided into CCl₄ control group (n = 10), Yi Guan Jian Decoction group (n = 9) and Xiao Chai Hu Decoction group (n = 9). Yi Guan Jian Decoction and Xiao Chai Hu Decoction were oral administrated per day respectively for 4 weeks, concomitantly continued CCl₄ administration. At 12th weekend, the rats were sacrificed for sampling and detection of liver function, histological changes of liver tissue, liver tissue hydroxyproline content and expression of α-SMA, CD68, MMP-13, TIMP-1, TIMP-2, Caspase-12, HGFα, MMP-2, MMP-9 and hepatocyte apoptotic index.

Results and conclusions

(1) Compared with that of normal rats, expression of α-SMA, CD68 and TIMP-1 in liver tissue of 8 week model group rats increases significantly (P < 0.01), moreover further increased in the 12 week of model group. However, MMP-13, HGFα, TIMP-2 content decreases gradually and the statistical difference is seen between each time point (P < 0.01). Activity of MMP-2, MMP-9, content of Caspase-12 and hepatocyte apoptotic index increased gradually at 4th, 8th, 12th week. (2) Compared to that of the same time point model group, activity of MMP-9 and contents of MMP-13, TIMP-2 and HGFα in Yi Guan Jian Decoction group improves significantly (P < 0.01), and activity of MMP-2 and contents of α-SMA, TIMP-1, Caspase-12 and hepatocyte apoptotic index decreases significantly (P < 0.01). This work suggests that Yi Guan Jian Decoction exerts significant therapeutic effect on CCl₄-induced cirrhosis in rats, through mechanism of inhibiting hepatocytes apoptosis and hepatic stellate cells activation, and regulating the function of Kupffer cell.

Ethnopharmacological relevance

This study investigates the mechanism of Yi Guan Jian against cirrhosis from aspect of heptocytes apoptosis and hepatic stellate cells activation. It suggest that although of unknown bioactive ingredients, mechanism of traditional Chinese medicine recipe against cirrhosis can be disclosed and of profound significance.     

针刺对健康大学生关脉脉图参数影响分析

运用脉像仪观察针刺前后脉图的变化。90例健康大学生配对分成3组，分别观察针刺曲泉、阴陵泉、阑尾穴前后两关脉脉图参数变化。结果：针刺曲泉穴后右关脉脉图主波波幅明显增高(P＜0．05)；针刺阴陵泉穴后左关脉脉图主波波幅显著增高(P＜0．01)，右关脉脉图降中波波幅明显增高(P＜0．05)；两组左右关脉脉图的脉动周期均明显延长，以左心室舒张期延长为主(P＜0．05)；左心室舒张期在前组明显延长，P＜0．05，而在后组则明显缩短，P＜0．05，且而组间有明显差异。针刺阑尾穴后两关脉脉图的参数无明显变化。提示针刺肝脾经腧穴在关脉部位上有不同表现，即支持寸口六部分候法，与传统的“左关候肝”，“右关候脾”不完全吻合。     

Observation of therapeutic effect of cervicogenic headache treated by acupuncture plus Tuina therapy

目的：观察电针配合推拿治疗颈源性头痛的疗效。方法；将200例颈源性头痛患者随机分为电针配合推拿组（针推组，n=100）、电针组（n=50）和推拿组（n=50），分别应用电针颈部夹脊穴、风池穴、阿是穴、患侧率谷、翳风、头维和外关穴和推拿治疗。结果：针推组有效率为100％；电针组总有效率为90．oH；推拿组总有效率为88．0％，差异有显著性意义（P〈0．05）。结论：电针结合推拿是治疗颈源性头痛的有效方法。     

Participation of local exosomes of acupoints in the initiation of acupuncture analgesic effect

Objective

To investigate the local initiation role of local exosomes of acupoints in acupuncture analgesic effect.

穴位埋线治疗不随意运动型脑瘫随机平行对照研究

[目的]观察穴位q埋线治疗不随意运动型脑瘫疗效。[方法]使用随机平行对照方法,将60例住院及门诊患儿按就诊顺序编号法随机分成两组。常规康复治疗：①运动疗法：Bobath法及Vojta法。②按摩。③作业疗法。④物理疗法：a水疗,b蜡疗,c小儿经络导平治疗仪。对照组30例传统针刺经穴法,取肩髃、曲池、外关、八邪、秩边、居髎、伏兔、足三里,1次/d。治疗组30例穴位埋线,取风池次、天髎次、臑会次、天宗次、承山次和伏兔次,1次/10d。连续治疗1个月为1疗程,观测临床症状、粗大运动功能、肌张力波动、不良反应。连续治疗3疗程,判定疗效。[结果]治疗组显效16例,有效8例,无效6例,总有效率为80.00%。对照组显效5例,有效15例,无效10例,总有效率为66.67%。临床疗效治疗组优于对照组（P〈0.05）。粗大运动功能均有改善（P〈0.01,P〈0.05）,治疗组优于对照组（P〈0.05）。缓解肌张力波动均有改善（P〈0.05）,治疗组优于对照组（P〈0.05）。[结论]穴位埋线治疗不随意运动型脑瘫能显著提高不随意运动型小儿脑瘫的粗大运动功能,缓解肌张力波动,值得在临床上广泛推广。     

曲马多加强针刺对关节炎大鼠的镇痛作用

目的 :观察不同剂量曲马多对关节炎大鼠热痛敏的镇痛作用及曲马多对针刺镇痛的影响。方法 :在完全弗氏佐剂关节炎大鼠模型上采用辐射热缩腿反射的方法 ,以痛敏分数作为观察指标 ,分别腹腔注射 (i.p .) 5mg·kg- 1 ·d- 1 、1 0mg·kg- 1 ·d- 1 、2 0mg·kg- 1 ·d- 1 曲马多和 1 0mL·kg- 1 ·d- 1 生理盐水 1 0天 ,记录每次给药 2 4hr后痛敏分数变化。继而选择 1 0mg·kg- 1 ·d- 1 曲马多与电针合用 ,观察单独应用 1 0mg·kg- 1 ·d- 1 曲马多、单独应用电针、曲马多与电针合用及1 0mL·kg- 1 ·d- 1 生理盐水四组 (均为 1 0天 ) ,每次给药或 /和电针 2 4hr后痛敏分数变化。结果 :i.p .5mg·kg- 1 ·d- 1 曲马多连续应用 1 0天时 ,大鼠痛敏分数无明显变化 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多 9天时大鼠痛敏分数显著提高 ;i.p .2 0mg·kg- 1 ·d- 1 曲马多 6天时大鼠的痛敏分数显著提高 ;单独应用电针 8天时大鼠痛敏分数显著提高 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多与电针合用 4天时可明显提高大鼠痛敏分数。结论 :长期应用曲马多对慢性炎症痛大鼠具有镇痛作用 ,呈剂量相关性 ;曲马多与电针合用可加强电针的镇痛作用 ,并降低曲马多使用剂量。提示曲马多与电针合用是一种治疗慢性痛的有效方法     

海马在针刺抑制内脏痛反应中的作用

<正> 针刺可以影响海马的自发电活动,也可以影响海马的诱发电活动。外周的伤害性刺激信号和针刺信号都可以到达海马,并且发生会聚和阻塞,提示海马可能参与针刺镇痛。针刺抑制内脏伤害性刺激反应(简称“内脏痛反应”)的作用已在多种动物模型上观察到,也为一些临     

Effects of different frequencies of electro-acupuncture at shuigou (GV 26) on recovery of motor function in rats with focal cerebral ischemic injury

Objective

To investigate the effects of different frequencies of electro-acupuncture at Shuigou (GV 26) on the latent period and wave amplitude of motor evoked potentials (MEPs) in rats with focal cerebral infarction.

Methods

Fifty healthy male Wistar rats were randomly divided into five groups: controls, model, 2 Hz Shuigou, 50 Hz Shuigou and 100 Hz Shuigou. There were 10 rats in each group. Using a modification of a technique for middle cerebral artery occlusion, focal cerebral ischemic injury was induced in all rats except those in the control group. The rats in the control group received no treatment. After behavioral deficit had been evaluated using the Zausinger 6-point neurological function score, the rats in the Shuigou groups underwent acupuncture and continuous wave stimulation at a frequency of 2 Hz, 50 Hz or 100 Hz (intensity 1 mA) for 10 min twice daily for 3 days. The control and model groups underwent no intervention. Zausinger 6-point neurological function score and MEPs were measured 72 h after the start of treatment.

Results

The neurological function scores of the three Shuigou groups were significantly higher than those of the model group (P<0.05). There was no significant difference between sides in the latency and amplitude of MEPs in the model group (P>0.05). The latency on the affected side in the model group was significantly longer than that in the control group (P<0.05) and the amplitude on affected side was significantly reduced (P<0.01). After 3 days of electro-acupuncture, the latency on the affected side in the 2 Hz Shuigou group was significantly shortened (P<0.05) and the amplitude was significantly increased (P<0.05) compared with the model group.

Conclusion

Low frequency electro-acupuncture at Shuigou (GV 26) can promote recovery of motor function after focal cerebral ischemic injury in rats.     

针灸内关、合谷、足三里穴在COPD合并消化不良中的治疗价值探讨

目的:观察在慢性阻塞性肺疾病(COPD)合并消化不良中应用针灸内关、合谷、足三里穴方案,对消化不良症状、临床疗效及肺功能的影响.方法:选择2018年12月-2019年12月我院收治的100例COPD合并消化不良患者的临床资料,50例单纯给予盐酸伊托必利胶囊治疗,作为对照组;50例除给予盐酸伊托必利胶囊外,另给予针灸内关...     

Effect of Electroacupuncture on Experimental Epilepsy： Roles of Different Acupoints and Stimulation Parameters

目的:确定电针对实验性癫痫的抑制效应,探索治疗实验性癫痫的最佳电针条件.方法:在雄性SD大鼠侧脑室注射红藻氨酸以诱导急性实验性癫痫.造模30分钟后,以1 mA/100 Hz和1 mA/10 Hz电针分别在水沟 大椎、筋缩 腰奇、内关 曲池、丰隆 涌泉四组穴位施治,以Racine行为学评分和脑电记录作为疗效评价的方法.结果:行为学评分结果表明:除1 mA/10 Hz电针内关 曲池外,低频(10 Hz)和高频(100 Hz)电针均可抑制实验性癫痫的发作(和对照组比较尸<0.05或0.01);2)在低频电针(10 Hz)治疗组中,丰隆 涌泉穴位的治疗效果优于其他穴位组(P<0.05或0.01);3)高频电针(100 Hz)治疗组中,不同穴位之间的疗效差异不明显;4)总体而言,除筋缩 腰奇穴位组外,高频电针(100Hz)的治疗效果优于低频电针(10 Hz)(P<0.05或0.01).与此同时,脑电图记录也显示出电针具有降低实验性癫痫大鼠脑电频率和幅度等的作用.结论:电针可以减轻实验性癫痫大鼠的癫痫发作,其效应与电针参数和穴位的选择有一定的相关性.