主动脉内球囊反搏术于急诊PCI中的应用

目的探讨主动脉内球囊反搏（IABP）支持下急性心肌梗死（AMI）合并心源性休克（CS）患者行急诊经皮冠状动脉介入术（PCI）治疗的临床疗效。方法回顾性分析28例急性心肌梗死并心源性休克患者,于发病0．5～12h内在IABP支持下行急诊冠状动脉造影,对梗死相关血管（IRA）直接行PTCA及支架置入。监测IABP置入对血流动力学参数的影响,并于4周内行心脏超声检查及心功能测定。结果冠状动脉造影示：单支血管病变2例,双支血管病变6例,三支血管病变18例,左主干病变2例。28例患者梗死相关动脉全部再通,23例患者血流达到TIMI3级,5例患者发生慢血流或无复流现象。急诊介入术中死亡2例,介入治疗术后住院期内死亡10例。住院期间总死亡率为42．9％（12／28）。16例存活患者4周时检查射血分数（EF）为0．43-0．67（0．53±0．10）。结论应用IABP可为急性心肌梗死合并心源性休克的患者在行急诊PCI中提供稳定的血流动力学支持。     

经桡动脉急诊PCI治疗急性前壁心肌梗死临床体会

目的:探讨经桡动脉途径行急诊冠状动脉介入(PCI)治疗对急性前壁心肌梗死(AMI)患者的临床疗效及安全性。方法:对35例急性ST段抬高性心肌梗死(前壁)在起病12h内进行急诊冠状动脉造影,对病变适合者行支架置入术,术后即刻行血管造影进行评价。结果:30例患者前降支动脉(LAD)开通后血流为TIMI3级,基本无残余狭窄,4例患者LAD开通后血流TIMI2级,1例患者因左主干闭塞术中死亡,1例患者术后1小时内因心脏破裂死亡,3例患者术后合并左心功能衰竭,5例患者择期行其它血管PCI术,住院期间33例患者无再次急性心肌梗死事件发生,出院患者随访1年,33例患者均无心血管事件发生。结论:经桡动脉途径急诊PCI治疗急性前壁心肌梗死临床疗效安全、可靠,相关并发症少,术后患者不受体位限制。     

急诊经皮冠脉腔内成形术及支架植入术治疗AMI的临床研究

目的：探讨急诊经皮冠状动脉腔内成形术(PTCA)及支架植入术(Stenting)治疗急性心肌梗死的有效性及安全性。方法：选择2001年4月至2003年1月期间75例急性心肌梗死患者，行急诊PTCA及支架植入术。结果：75例患者中74例(98．7％)支架被成功植入靶血管病变部位；1例急性广泛前壁心肌梗死合并心源性休克的病人经抢救无效死亡。结论：急诊经皮冠状动脉腔内成形术(PTCA)及支架植入术(Stenting)治疗急性心肌梗死安全、有效．应为首选。     

直接经皮冠脉内支架术治疗急性心肌梗死临床分析

目的：评价急性心肌梗死(AMI)患者梗死相关动脉行急诊冠状动脉内支架术的疗效及安全性。方法：对24例急性心肌梗死患者在发病12h内作梗死相关动脉直接经皮冠状动脉腔内形成术(PTCA)加冠状动脉内支架置入术。梗死相关动脉：前降支14例．回旋支4例，右冠状动脉6例。结果：24例患者中23例介入治疗成功(成功率95．8％)，梗死相关动脉共置人支架25个．TIMI血流3级且无围手术期严重并发症(死亡、急性心肌梗死、急诊冠状动脉旁路移植术)。随访1～30个月．1例术后2个月出现心绞痛．经冠脉造影证实为再狭窄，经再次PTCA治疗成功。结论：急诊冠状动脉内支架术对急性心肌梗死患者是一种安全有效的治疗措施。     

国产替罗非班对急性心肌梗死急诊介入术后近期疗效和安全性的研究

目的：研究国产血小板膜糖蛋白Ⅱb／Ⅲa受体拮抗剂替罗非班（欣维宁）在治疗急性心肌梗死（AMI）患者急诊经皮冠状动脉介入（PCI）术后的疗效和安全性。结果：30天主要不良事件发生率4．9％，术后即刻IRA的TIMI3级血流获得率95％，严重出血发生率0％，轻微出血发生率7．4％。结论：替罗非班在急性心肌梗死的标准治疗基础上能进一步减少心肌缺血事件，改善PCI术后的冠脉血流，同时又具有较好的安全性。     

急性心肌梗死介入治疗96例临床分析

目的:研究急诊介入治疗急性心肌梗死的有效性和安全性。方法:对发病<24h的96例急性心肌梗死患者行急诊经皮冠脉介入术(PCI)。结果:介入治疗获得成功90例,无心血管事件发生。术后住院期间死亡4例,病死率4.1%。术后再发心肌梗死2例,再次PCI成功。结论:急性心肌梗死行急诊PCI安全、有效、成功率高、并发症少,可改善预后。     

急诊经皮冠状动脉介入治疗急性心肌梗死28例

目的总结急诊经皮冠状动脉介入(PCI)治疗急性心肌梗死(AMI)的疗效。方法采用急诊PCI技术治疗AMI 28例,对临床疗效及安全性进行观察。结果 28例AMI患者均顺利完成急诊冠状动脉造影术,并同时行支架植入。支架植入后,残余狭窄均≤20%,TIMI血流Ⅲ级,计26例,占92.9%。围手术期无死亡或再梗死等严重心脏事件。结论 PCI能安全有效的开通梗死相关动脉(IRA)。     

急诊冠状动脉内支架术治疗ST段抬高急性心肌梗死

目的 评价急诊冠状动脉内支架术治疗急性心肌梗死(AMI)的安全性和临床疗效。方法 选择自1999年12月至2002年12月间我院69例急性心肌梗死患者，起病12小时内，所有病例均未行静脉溶栓治疗，即刻送导管室行冠状动脉造影术，明确梗死相关动脉(IRA)，球囊预扩张后，冠状动脉内置入支架。结果 69例急性心肌梗死患者中，左前降支病变刃例，左回旋支病变2例，右冠状动脉病变28例，原则上干预梗死相关动脉(IRA)，共置入支架72枚，手术成功率100％，所有患者支架置入后TIMI血流3级67例，2级2例。术后无心脏事件发生。结论 急性心肌梗死患者行急诊冠状动脉内支架术安全有效，且效果优于溶栓治疗。     

急性心肌梗死心肺复苏后急诊介入治疗12例临床分析

目的：探讨急性心肌梗死（acute myocardial infarction,AMI）、心肺复苏（Cardiopulmonary Resusci-tation CPR）后急诊经皮冠状动脉介入治疗（percutaneous coronary interventions,PCI）的疗效。方法：12例有心肺复苏史接受急诊经皮冠状动脉介入治疗急性心肌梗死的患者,对心肺复苏的发生时间、心肺复苏所需时间、冠状动脉造影结果、急诊经皮冠状动脉介入术后病人病情稳定时间、病人总住院时间、术后1周的左室射血分数（LVEF）进行回顾性分析。结果：心肺复苏所需时间212（5.2±2.0）min。梗死相关血管左前降支近、中段闭塞7例,右冠状动脉近、中段闭塞4例,回旋支近段闭塞1例,多支病变8例,单支病变4例,梗死相关动脉均置入支架1枚,前向血流TiMi 3级。急诊经皮冠状动脉介入术后病人病情稳定时间为术后112 h、病人总住院时间为（10.15±3.42）d、术后1周的左室射血分数为（60.35±10.03）。结论：急诊经皮冠状动脉介入治疗心肺复苏后急性心肌梗死是一种安全有效的方法。     

经桡动脉途径急诊PCI治疗的安全性临床分析

目的探讨经桡动脉途径急诊经皮冠脉介入治疗（PCI）的安全性及可行性。方法回顾性分析皖北煤电集团总医院2006年11月-2009年6月对76例急性心肌梗死患者采用经桡动脉途径行急诊PCI治疗的临床资料。观察造影成功率、入院-球囊扩张时间、梗塞相关动脉的开通率、血流情况、术中并发症,住院期间主要心血管事件。结果经桡动脉造影成功率为100%;入院-球囊扩张时间（55±18）min;梗塞相关动脉的开通率为97.4%;血流情况：TIMI3级93.4%;皮下瘀斑的发生率为3.8%;前臂血肿的发生率为2.9%;桡动脉闭塞的发生率为1.0%;平均术后住院天数（5.9±1.4）d;2例出现围手术期死亡。结论经桡动脉途径直接PCI治疗急性心肌梗死安全有效,在有条件的医院可首选经桡动脉途径PCI作为急性心肌梗死（AMI）治疗的方法。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.