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1.
以21月龄小鼠为自然衰老组,3月龄小鼠随机分为给药组和对照组,观察咖啡因对实验小鼠各组织脂褐质含量和血清过氧化脂质(Lpo)含量以及血清中超氧化物歧化酶(SOD)活力和脑组织B型单胺氧化酶(MAO-B)活力的影响。结果显示,咖啡因能显著提高3月龄小鼠脑、心、肝等组织脂褐质含量(P<0.01或P<0.05)以及血清Lpo含量(P<0.01),咖啡因使小鼠血清SOD活力下降(P<0.01或P<0.05),但使脑中MAO-B活力显著升高(P<0.01或P<0.05),还能使胸腺萎缩,重量减轻(P<0.01)。除胸腺外,上述指标均接近21月龄小鼠水平。结果提示,咖啡因有致衰老作用。  相似文献   

2.
参黄冲剂对小鼠SOD、MAO-B活性的影响黑龙江中医学院樊景坡,孙巧红参黄冲剂是以补肾填精、健脾益气为主,和现代医学相结合组成的复方制剂。实验证明,它能明显地延长果蝇的平均寿命及半数致死时间,并能降低老龄小鼠心、肝、肾过氧化脂质含量,减少脑脂褐素的沉...  相似文献   

3.
用^3H-TdR掺入法,测定受分次γ线照射的小鼠脾淋巴细胞转化功能,同时测定受照小鼠脑,肺,肝,脾SOD活力和LPO含量,结果表明,榄香烯提高了小鼠T,B淋巴细胞的辐射抗性(P〈0.05),以及脾,肝,肺SOD活力(P〈0.01,P〈0.05),降低脾LPO含量(P〈0.01),提示榄香烯的辐射免疫保护机理之一,是由于增强组织中SOD活力,降低LPO含量,从而减少自由基及有害物质对淋巴细胞的损伤。  相似文献   

4.
报道5Gy60Coγ射线照射后1、3、5、8d对小鼠心、脑、肝组织中LPO含量和SOD活力的影响,以及小鼠受照射后,立即由其腹腔注射外源性SOD,观察在上述4个时间点3种组织中LPO含量改变情况。结果表明:小鼠受照后,其肝脏中LPO含量最早发生变化,而心、脑LPO含量在照后第8天才出现明显改变。同时上述组织中的SOD活力,与对照组相比也明显下降(P<0.01)。注入SOD后,照后不同时间测定LPO含量、SOD活力与对照组相比,分别降低和升高,说明SOD酶具有明显的抗辐射作用。  相似文献   

5.
不同声强8Hz次声作用对大鼠GSH-PX,SOD活性及MDA含量的影响   总被引:9,自引:4,他引:5  
目的:观察不同声强的8Hz次声较长时间作用大鼠后,脑心肝肺的GSH-PX,SOD活性及MDA含量的变化以探讨次声作用对组织细胞的影响.方法:用8Hz90dB,120dB次声作用,每日2h,14d后观察大鼠脑肝心肺组织中GSH-PX活性,SOD活性及MDA含量的变化.结果:大鼠脑肝肺组织中GSH-PX活性较对照组均有显著降低(P〈0.05,P〈0.01).而心肌组织中GSH-PX活性变化不明显.大鼠  相似文献   

6.
本实验研究了连续服用茶多酚20天,对14月龄小鼠血及肝中SOD、GSH-Px和LPO的影响。结果表明茶多酚能提高血及肝中SOD及GSH-Px活性,与正常对照组比较P<0.01,同时能降低血及肝中LPO含量,与正常对照组比较P<0.01。提示茶多酚可通过降低机体自由基水平,起到抗衰老作用。  相似文献   

7.
本实验研究了连续服用茶多酚20天,对14月龄小鼠血及肝中SOD、GSH-Px和LPO的影响。结果表明茶多酚能提高血及肝中SOD及GSH-PX活性,与正常且比较P〈0.01,同时能降低血及肝中LPO含量,与正常对照组比较P,0.01。提示茶多酚可通过降低机体自由基水平,起到抗衰老作用。  相似文献   

8.
外源性SOD减轻大剂量照射对小鼠的损伤效应   总被引:2,自引:0,他引:2  
报道5Gy^60Coγ射线照射后1、3、5、8d对小鼠心,脑、肝组织加LPO含量和SOD活力的影响,以及小鼠受照后,立即由其腹腔注射外源性SOD含量最早发生变化,而、心、脑LPO含量在照后第8天才出现明显改变。  相似文献   

9.
合成衣原体属特异脂多糖(LPS)的四糖链α-2-酮基-3-脱氧辛酸(KDO)(2→8)-α-KDO(2→4)-α-KDO(2→6)-β-GlcNAc与牛血清白蛋白(BSA)共价结合,制成糖-BSA结合物抗原,用其免疫小鼠所制备的单克隆抗体(McAb)经间接血凝试验、血凝抑制试验、ELISA、免疫荧光试验和免疫印渍等试验分析,结果把这些McAb分为两组:一组与衣原体LPS和Re型LPS有交叉反应;另一组为衣原体特异性抗体,其中KDO3特异抗体与衣原体原体McAb有共同决定簇特异性,但亲和力却高于后者100多倍。表明合成糖-BSA结合物与衣原体LPS有相似的抗原性。  相似文献   

10.
作者测定了25例糖尿病微血管病变和12例无微血管病变患者血中SOD-T、SOD-Mn、SOD-Cu·Zn、VE、LPO的含量。结果显示,糖尿病患者血SOD-T、SOD-Mn、SOD-Cu·Zn含量明显减低(P<0.01),LPO明显增高(P<0.01),VE含量无明显变化。但微血管病变组与无微血管病变组血中SOD和LPO含量无明显差异。提示糖尿病微血管病变患者血中SOD、LPO的含量变化系糖尿病本身的变化特征,而不是糖尿病微血管病变的结果。  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

15.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.  相似文献   

17.
Objective:To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas(SPTP). Methods:Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin(HE) staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results:Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin(9/9 cases), AAT(9/9 cases), NSE(9/9 cases), ACT(7/9 cases), CK20(2/9 cases), CgA(1/9 cases), S-100(3/gcases), PR(4/gcases), Syn(3/9 cases) and CD56(5/9cases), negative in CEA and ER. Conclusion:SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.  相似文献   

18.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

19.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

20.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

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