The effect of cumin supplementation on metabolic profiles in patients with metabolic syndrome: A randomized,triple blind,placebo‐controlled clinical trial

Metabolic syndrome (MetS) is a cluster of interconnected serious disorders, which is a major health problem whose prevalence is increasing. Oxidative stress and inflammation contribute to the disease pathogenesis and its complications. The present study aimed to investigate the effect of Cuminum cyminum L. (which has antioxidant and anti‐inflammatory properties) essential oil (CuEO) supplementation on inflammatory and antioxidant status in patients with MetS. In this clinical trial, 56 patients with MetS aged 18–60 years received either 75‐mg CuEO or placebo soft gel, thrice daily, for 8 weeks. Data on anthropometric parameters, food consumption, tumor necrosis factor alpha, high‐sensitivity C‐reactive protein, superoxide dismutase (SOD), glutathione peroxidase, catalase, total antioxidant capacity (TAC), and malondialdehyde (MDA) were assessed at the beginning and at the end of the study. Compared with the placebo group, CuEO increased SOD (149.17; 95% CI, [67.93, 230.42]), TAC (0.24; 95% CI, [0.09, 0.38]) and decreased MDA (?0.36; 95% CI, [?0.66, 0.06]), (p < 0.01). In within‐group analysis, CuEO led to 13.3% decrease in MDA and 6.7% increase in TAC levels (p < 0.04). The results indicated that CuEO supplementation can improve some antioxidative indices, as SOD and TAC, while decreasing MDA in patients with MetS.     

The effect of sumac supplementation on insulin resistance,inflammation, oxidative stress,and antioxidant capacity in adults with metabolic syndrome: A randomized crossover clinical trial

The impact of sumac supplementation was not previously examined in patients with metabolic syndrome (MetS). So, we investigated the influence of sumac supplementation on insulin resistance, inflammation, oxidative stress, and antioxidant markers in adults with MetS. The current study was a single-center, triple-blind, randomized, placebo-controlled crossover clinical trial that was conducted on 47 patients with MetS. Participants were randomly divided into two groups of intervention and control in the first phase. They received capsules containing 500 mg sumac or placebo (lactose) twice a day, after lunch and dinner, for 6 weeks. After a 2-week washout period, the subjects crossed over to the alternate arm. In both arms, fasting venous blood samples were obtained at baseline and at the sixth week of the intervention to determine glycemic status, high-sensitivity C-reactive protein (hs-CRP), malondialdehyde (MDA), total antioxidant capacity (TAC), and antioxidant enzymes activity. The mean (±SD) age, weight, and body mass index of participants were respectively 58.7 ± 5.83 years, 79.9 ± 14.35 kg, and 31.6 ± 4.6 kg/m²; 80.9% of subjects were females (n = 38). Based on per-protocol analyses, the serum fasting insulin concentration was unexpectedly decreased in the placebo group (change in sumac vs. control: 0.95 ± 3.9 vs. −1.17 ± 3.9 mU/L; p = 0.04). Sumac supplementation, compared to control, has marginally enhanced catalase activity (change in sumac vs. control: 2.91 ± 9.5 vs. −1.07 ± 9.1 nmol/min/ml; p = 0.06). This supplementation has also increased serum TAC and the activity of superoxide dismutase, but these changes were not significant. This intervention did not affect other insulin resistance, inflammatory, or oxidative stress markers. We found that 1 g/day sumac consumption for 6 weeks, as adjuvant therapy, could improve the antioxidant defense system in adults with MetS but did not affect insulin resistance, inflammation, or oxidative stress.     

复方丹参片对维持性血液透析患者氧化应激和微炎症状态的干预作用

目的 研究复方丹参片对维持性血液透析（MHD）患者氧化应激和微炎症的影响。方法 36例MHD0．5年以上的患者随机分为常规透析组和透析加丹参组,同时18名健康成人作为正常对照组。分别测定各组基础以及透析组1、2、3个月的血浆氧化应激指标（AOPP、MDA、S（）D）和微炎症指标（CRP、IL6、TNF-α）的水平。结果透析患者的CRP、IL-6、TNF-α、AOPP、MDA明显高于正常对照组（P〈0．01）、SOD显著低于正常对照组（P〈0．01）。常规透析组3个月时AOPP、MDA较服药前增加（P〈0．05）,EC）D降低（P〈0．05）,CRP、IL-6、TNF-α增加但无显著性差异（P〉0．05）。透析加丹参组在1、2、3个月时MDA、AOPP明显低于服药前以及同时点常规透析组（P〈0．01）,而SOD则显著增高（P〈0．01）,第3个月所测CRP低于服药前（P〈0．05）以及同时点常规透析组（P〈0．01）,但IL-6、TNF-α降低不明显（P〉0．05）,透析加丹参组各时点的CRP、IL-6、TNF-α、AOPP、MDA仍高于正常对照组（P〈0．01）,SOD低于正常对照组（P〈0．01）。结论 血液透析可能加重MHD患者的氧化应激和微炎症,复方丹参片能有效地改善MHD患者的氧化应激和微炎症状态。     

人参皂苷Rb1对自发性高血压大鼠氧化应激指标的影响

目的 观察人参皂苷Rb1对自发性高血压大鼠（SHR）血压及氧化应激指标的影响.方法 选择12周龄雄性SHR 36只,随机分为4组,人参皂苷Rb1大剂量和小剂量组、空白组（生理盐水）、阳性对照组（氨氯地平）.血压正常的京都Wistar Kyoto（WKY）大鼠9只为正常对照组.于治疗第0周、12周分别用尾袖法测定大鼠动脉收缩压,并分别采血,检测超氧化物歧化酶（SOD）、丙二醛（MDA）及氧化低密度脂蛋白（ox-LDL）等指标.结果 人参皂苷Rb1作用12周后,大、小剂量组血压均较治疗前明显下降,但较氨氯地平组差;Rb1高剂量组及氨氯地平组分别与生理盐水对照组比,差别均有统计学意义（P＜0.05）.针对氧化应激指标的检测发现,人参皂苷Rb1作用后,MDA,SOD以及ox-LDL水平都有一定程度的改善,尤其以MDA和ox-LDL改变最为显著,与生理盐水组比,差别具有统计学意义（P＜0.05）.结论 人参皂苷Rb1具有较为显著的抗氧化作用,其降血压效应部分与其对抗氧化应激作用有关.     

黄芪注射液对高果糖饮食诱导的大鼠胰岛素抵抗和氧化应激的影响

目的:探讨黄芪注射液对高果糖饮食大鼠胰岛素抵抗和氧化应激的抑制作用.方法:SD大鼠经高果糖饲料喂养4周后,随机分为模型组,黄芪注射液高、低剂量(相当于生药量ip 5,2.5 g·kg-1)组、罗格列酮(3 mg·kg-1,ig)组,连续给药4周,继续给予高果糖饲料4周.同时设立正常组,普通饲料喂养.测定体重、空腹血糖、血浆胰岛素、胰岛素抵抗指数、氧化应激指标.结果:模型组体重与正常组无明显区别;空腹血糖、血浆胰岛素水平、胰岛素抵抗指数、丙二醛(MDA)含量明显高于正常组(P＜0.05);过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性显著低于正常组(P＜0.05).与模型组相比,黄芪注射液组、罗格列酮组空腹血糖、血浆胰岛素水平、胰岛素抵抗指数、MDA含量降低,CAT,SOD,GSH-Px活性升高,高剂量组与模型组比较有显著性差异(P＜0.05).结论:黄芪注射液可减轻高果糖饮食所引起的胰岛素抵抗及氧化应激.     

Anti-inflammatory activity of methanol extract of Kalopanax pictus bark and its fractions

Dried pulverized bark of Terminalia arjuna Linn (TA) was administered orally to Wistar albino rats (120-150 g) in two doses [500 and 750 mg/kg in 2% carboxy methyl cellulose (CMC)], 6 days per week for 12 weeks. Thereafter, rats were sacrificed either for determination of baseline changes in cardiac endogenous antioxidant compounds [superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT)] or the hearts were subjected to oxidative stress associated with in vitro ischemic-reperfusion injury (IRI). There was significant increase in the baseline contents of thiobarbituric acid reactive substance (TBARS) (a measure of lipid peroxidation) with both doses of TA. However, only in the 500 mg/kg treated group, this was accompanied by a simultaneous increase in SOD, GSH and CAT levels, but not in the 750 mg/kg treated group, where only CAT was raised. Significant rise in myocardial TBARS and loss of SOD, CAT and GSH (suggestive of increased oxidative stress) occurred in the vehicle-treated hearts subjected to in vitro IRI. Only hearts, harvested from the 500 mg/kg rats treated rats, were significantly protected from oxidative stress, when subjected to in vitro IRI. The results suggest that crude bark of TA augments endogenous antioxidant compounds of rat heart and also prevents oxidative stress associated with IRI of the heart.     

大蒜素对2型糖尿病大鼠氧化应激反应的影响

目的观察大蒜素对2型糖尿病大鼠氧化应激反应的影响,并探讨其发生机制。方法取健康雄性Wistar大鼠40只,随机分为正常组10只和糖尿病造模组30只。以高热量饮食+链脲佐茵素法建立糖尿病大鼠模型,将造模成功的26只再随机分为2组,即单纯糖尿病组13只,糖尿病+大蒜素组13只。糖尿病+大蒜素组喂高脂高糖饲料加大蒜素3周。测定大鼠FPG、血清XOD、SOD、MDA和TNF-α、CRP、PAI-1及IL-6水平的变化,观察大蒜素对2型糖尿病大鼠氧化应激反应的干预作用。结果与正常组比较,糖尿病大鼠空腹血糖升高,血浆SOD活性降低而MDA、XOD含量增加,血浆TNF-α、PAI-1及IL-6水平升高,均具有显著性差异(P0.05或0.01)。服用大蒜素后,空腹血糖的升高、血浆SOD的减少和MDA、XOD含量的增加均受到一定程度抑制,血浆TNF-α、PAI-1及IL-6水平也有所降低,具有显著性差异(P0.05或0.01)。结论大蒜素对2型糖尿病大鼠有一定降血糖效应,其机制可能是通过抗氧化、抑制炎症反应而减轻氧化应激损伤的程度。     

解痉通络活血方联合西药对高血压左心室肥厚患者氧化应激及血管内皮细胞功能的影响

目的:观察解痉通络活血方联合西药对高血压左心室肥厚(left ventricular hypertrophy,LVH)患者氧化应激及血管内皮细胞功能的影响.方法:将117例LVH患者按照随机数字表法分为对照组57例和观察组60例.对照组采用常规西药治疗,观察组在对照组的基础上联合解痉通络活血方治疗.比较两组患者治疗前后...     

血管紧张素Ⅱ-NADPH氧化酶-活性氧通路在慢性心肌缺血血瘀证中作用的实验研究

目的:观察血管紧张素Ⅱ-NADPH氧化酶(NADPH oxiase)-活性氧通路在慢性心肌缺血血瘀证发生发展中的作用。方法:采用中华小型猪左冠状动脉前降支放置Ameroid缩窄环的方法制备慢性心肌缺血血瘀证模型。小型猪随机分为假手术组、模型组,动态观察模型动物心肌缺血(0-4周)四诊体征与血液流变学指标,采用冠脉造影测定心肌缺血的程度;Elisa方法测定血浆血管紧张素Ⅱ(AngⅡ)、醛固酮(Ald)含量以及氧化应激关键分子NADPH氧化酶、活性氧(ROS)、超氧物歧化酶(SOD)的浓度变化。结果:该模型在4周确定为稳定的病证结合慢性心肌缺血血瘀证模型,血瘀证形成时肾素-血管紧张素-醛固酮系统(RAAS)中,AngⅡ和Ald分别上升了14.10%和56.25%。NADPH氧化酶和代表ROS含量变化的丙二醛(MDA)分别上升了43.75%和9.24%,SOD下降了11.55%(P<0.05),相关性分析发现,AngⅡ与NADPH氧化酶呈正相关(P<0.05),NADPH氧化酶与MDA呈正相关(P<0.05),AngⅡ与MDA呈正相关(P<0.01)。结论:该模型制备的慢性心肌缺血血瘀证,早期的病理机制包括RAAS系统的激活以及氧化应激损伤反应。其中,AngⅡ-NADPH氧化酶-ROS之间存在着正相关关系。AngⅡ介导NADP氧化酶浓度的升高,进一步增加NADPH氧化酶来源的ROS生成,RAAS系统的激活与氧化应激反应互为因果,进而导致了血瘀证的临床改变,及早干预该环节可提高慢性心肌缺血血瘀证的临床疗效。     

麝香保心丸对冠心病不稳定型心绞痛患者氧化应激的影响

目的:探讨麝香保心丸对冠心病不稳定型心绞痛患者的疗效及对氧化应激的影响.方法:84例冠心病不稳定型心绞痛患者随机分为对照组和观察组各42例.两组均采用常规抗栓和稳定斑块等基础治疗,对照组服用单硝酸异山梨酯片,10 rg/次,3次/d;观察组在此基础上加服麝香保心丸,2粒/次,3次/d.疗程均为8周.若仍有心绞痛发作者,两组均临时含服硝酸甘油片0.5 mg/次.检测治疗前后血清超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(TAC)、脂质过氧化物(LPO)水平,记录心绞痛发作次数、硝酸甘油用量.结果:治疗后观察组总有效率为95.24％,优于对照组的80.95％(P＜0.05);治疗后观察组平均每天心绞痛发作次数及硝酸甘油用量均明显低于对照组(P＜0.05);两组治疗后SOD及TAC均较治疗前上升,观察组上升更明显(P＜0.01);两组治疗后MDA及LPO均较治疗前下降,观察组下降更明显(P＜0.01).结论:麝香保心丸对冠心病不稳定型心绞痛有较好的临床疗效,其作用机制可能与调节心肌细胞的氧化和抗氧化平衡有关.     

银杏叶注射液调节异氟烷所致高龄大鼠认知功能障碍的实验研究

目的:探讨银杏叶注射液对异氟烷所致高龄大鼠认知功能障碍及其对海马组织炎症、氧化应激的调节作用。方法:将36只SD大鼠按照随机数字表法分为对照组、异氟烷组和银杏叶组,每组各12只。异氟烷组及银杏叶组予以30%氧气和70%氮气作为载体气体,给予1.4%异氟烷6 h进行麻醉,对照组仅给予30%氧气和70%氮气。银杏叶组在麻醉前3 d给予银杏叶注射液2 mL·kg^-1腹腔注射,对照组、异氟烷组给予2 mL·kg^-1生理盐水腹腔注射。采用Morris水迷宫评价认知功能,检测海马组织中炎症指标[白细胞介素(interleukin,IL)-1β、IL-6、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)]、氧化应激指标[丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)和总抗氧化力(total antioxidant capacit,TAC)]的含量及p38 MAPK的蛋白表达。结果:异氟烷组大鼠的逃避潜伏期明显长于对照组,在原平台范围的停留时间明显短于对照组,穿过原平台所在位置的次数及海马组织中SOD、GPx、TAC的含量明显低于对照组,海马组织中IL-1β、IL-6、TNF-α、MCP-1、ROS、MDA的含量及p38 MAPK的蛋白表达水平均明显高于对照组,差异均有统计学意义(P<0.05)。银杏叶组大鼠的逃避潜伏期明显短于异氟烷组,在原平台范围的停留时间明显长于异氟烷组,穿过原平台所在位置的次数及海马组织中SOD、GPx、TAC的含量明显高于异氟烷组,海马组织中IL-1β、IL-6、TNF-α、MCP-1、ROS、MDA的含量及p38 MAPK的蛋白表达水平均明显低于异氟烷组,差异均有统计学意义(P<0.05)。结论:银杏叶注射液能够减轻异氟烷所致高龄大鼠认知功能障碍及海马炎症、氧化应激反应。     

Ameliorative effects of pycnogenol on carbon tetrachloride-induced hepatic oxidative damage in rats

This study evaluated the putative antioxidant activity of Pycnogenol (PYC) against CCl4-induced hepatic oxidative damage in Sprague-Dawley rats. A single oral dose of CCl4 (1.25 mL/kg) produced significantly increased levels of serum aminotransferase (AST) and alanine aminotransferase (ALT) activities. In addition, increased malondialdehyde (MDA) concentration, reduced glutathione (GSH) content, and decreased catalase, superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities were observed in the hepatic tissues. However, concomitant administration with PYC (10 or 20 mg/kg) significantly improved CCl4-induced hepatic injury, as evidenced by the decline of serum AST and ALT activities in a dose dependent manner. Moreover, PYC reduced MDA concentration and increased GSH levels and catalase, SOD and GST activities in hepatic tissues, indicating that concomitant administration with PYC efficiently prevent the CCl4-induced oxidative damage in rats. The free radical scavenging assay showed that PYC has a dose-dependent scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. These results indicate that PYC has an antioxidant effect against CCl4-induced hepatic oxidative damage and is useful as a hepatoprotective agent against various liver diseases induced by oxidative stress.     

Hepatoprotective effect of the aqueous extract of Flemingia macrophylla on carbon tetrachloride-induced acute hepatotoxicity in rats through anti-oxidative activities

This study investigated the protective effect of the aqueous extract of Flemingia macrophylla (AFM) against hepatic injury induced by CCl(4). Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected as biomarkers in the blood to indicate hepatic injury. Product of lipid peroxidation (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and reduced glutathione (GSH) contents were evaluated for oxidative stress in hepatic injury. Moreover, histopathological observation was assayed for the degree of hepatic injury. After oral administration of AFM, 0.5 g/kg and 1.0 g/kg doses significantly decreased ALT and AST, attenuated the histopathology of hepatic injury, ameliorated oxidative stress in hepatic tissue, and increased the activities of CAT, SOD and GSH-Px. The hepatoprotective effect of daidzein and genistein were consistent to that of AFM. This study demonstrated for the first time that AFM has hepatoprotective effect on acute liver injuries induced by CCl(4), and the results suggested that the effect of AFM against CCl(4)-induced liver damage was related to antioxidant properties.     

Antioxidant effect of indirect moxibustion on healthy subjects: a pilot study

Objective

Moxibustion has long been thought to promote vital energy or immunity through clinical experience. This study aimed to investigate the clinical effects of indirect moxibustion on antioxidant and immunomodulation in a normal population.

Methods

Twenty-five healthy volunteers with no objective or subjective disorders were recruited. The participants were treated with indirect moxibustion on acupoints Guanyuan (CV 4) and Shenque (CV 8) three times per week for 4 weeks. The serum levels of reactive oxygen species (ROS) and malondialdehyde (MDA), the total antioxidant capacity (TAC), the activities of catalase and superoxide dismutase (SOD), and the total glutathione content were determined before and after the 12th moxibustion. Lymphocyte subpopulations and 42 cytokines in the peripheral blood were analyzed using flow cytometry and antibody array, respectively.

Results

Compared with the initial time point, the serum levels of ROS and MDA were significantly lowered by moxibustion, while TAC was increased (P<0.01 for all). A significant increase was observed in catalase activity (P<0.05), but not in SOD or total glutathione. There were no significant changes in lymphocyte subpopulations or cytokines in the peripheral blood. Fifteen of 25 participants reported at least one symptom in which they felt subjective improvement after moxibustion.

Conclusion

Indirect moxibustion on acupoints CV 4 and CV 8 improved the antioxidant defense system, which may be a mechanism explaining the clinical effects of moxibustion.     

八味沉香散对缺血再灌注损伤心肌细胞SOD、MDA和凋亡影响的研究

目的观察八味沉香散对缺血再灌注损伤大鼠心肌细胞H9C2 SOD活力、MDA浓度以及细胞凋亡的影响。方法H9C2细胞随机分为6组:正常组、缺血再灌注组(I/R组)、八味沉香散提取物(水提)高、中、低剂量组(240、120、60μg/mL)、曲美他嗪组(240μg/mL)。以体外缺糖缺氧再复糖复氧建立缺血再灌注损伤模型,WST-1法检测细胞内SOD活力,TBA法检测MDA浓度,Hoechst 33258染色法检测细胞凋亡水平。结果模型组细胞受到缺血再灌注损伤刺激后,细胞内MDA浓度明显增高,SOD活力下降,荧光显微镜下有大量致密或碎块状的浓染细胞核,半定量图像分析平均光密度增高(P<0.01),与正常组比较有显著差异。与模型组比较,八味沉香散提取物中的MDA含量降低(P<0.01),而SOD活力显著增高(P<0.05,P<0.01),荧光显微镜下的致密浓染细胞核明显减少、半定量图像分析平均光密度显著降低(P<0.01)。结论八味沉香散能够减少脂质过氧化物(MDA)生成,提高具有抗氧化能力SOD的活力,抑制细胞凋亡。推测八味沉香散抗缺氧再灌注损伤是通过减少应激状态下活性氧化自由基的形成,从而阻断活性氧化自由基诱发细胞凋亡的直接和间接途径,达到保护心肌细胞的作用。     

青钱柳对2型糖尿病大鼠早期肾损伤标志物以及氧化应激的影响

目的探究青钱柳对2型糖尿病大鼠的中性粒明胶酶脂质运载蛋白(neutral gelatinase lipoprotein,NGAL)、肾损伤因子1(kidney damage factor 1,KIM-1)、铜蓝蛋白(ceruloplasmin,CP)以及氧化应激的影响。方法雄性SD大鼠100只,适应性喂养1周,将大鼠随机分为空白组10只和造模组90只。其中空白组给予普通饲料,造模组给予高脂饲料喂养8周。第10周,以30 mg/kg的剂量一次性腹腔注射2%链脲佐菌素(streptozotocin,STZ)(现配现用,避光取适量STZ,在0.1 mol/L柠檬酸-柠檬酸钠缓冲液冰浴中配置,pH=4.5)。72小时后选择空腹血糖>11.1 mmol/L的成模大鼠随机分为模型组,二甲双胍组,青钱柳水提物(Cyclocarya paliurus water extract,CPE)低、中、高剂量组,每组10只。空白组和模型组给予0.9%氯化钠溶液,二甲双胍组给予二甲双胍混悬液(0.3 g/kg),CPE低、中、高剂量组分别给予青钱柳叶水提取物[CPE(0.25、0.5、1 g/kg)]。连续给药4周,期间检测空腹血糖(fasting blood glucose,FBG)。给药4周后,检测肌酐(creatinine,CRE)、尿素氮(urea nitrogen,UREA)、NGAL、KIM-1、CP、丙二醛(malondialdehyde,MDA)、活性氧(reactive oxygen species,ROS)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、过氧化氢酶(catalase,CAT)、超氧化物歧化酶(superoxide dismutase,SOD)的含量。结果与模型组比:(1)CPE低、中、高剂量组FBG显著降低(P<0.05);(2)CPE低、中、高剂量组CRE、UREA、NGAL、KIM-1、CP显著降低(P<0.05);(3)CPE低、中、高剂量组MDA、ROS显著降低,GSG-PX、CAT、SOD显著升高(P<0.05)。结论青钱柳可有效缓解氧化应激损伤,从而防治2型糖尿病早期肾损伤。     

Concentration‐dependent effect of (−) epicatechin in hypertensive patients

Non‐vitamin polyphenolic compounds are ubiquitous in food plants and therefore potentially present in human plasma in a diet‐dependent concentration. The aim of this study was to evaluate the concentration‐dependent effect of (?) epicatechin, a polyphenol present in green tea with antioxidant activity, on various biomarkers of oxidative stress. The current study examined the in vitro concentration‐dependent (10^?4 M to 10^?7 M) effects of (?) epicatechin on biomarkers of oxidative stress viz. malondialdehyde (MDA), reduced glutathione (GSH), membrane sulfhydryl (‐SH) group and protein carbonyl content in hypertensive patients and normal ones. This effect seems to be due to ability of (?) epicatechin to reduce MDA and protein carbonyl content while increase in GSH and membrane ‐SH group in hypertensive patients. It can be concluded that (‐) epicatechin exerts an antioxidant action inside the cell, responsible for the observed modulation of cellular response to oxidative challenges. Copyright © 2010 John Wiley & Sons, Ltd.     

冠心康颗粒剂对镉中毒大鼠氧化损伤的保护作用

[目的]通过观察冠心康颗粒剂对镉中毒大鼠氧化损伤的影响,为研究环境毒邪所致衰老的机制以及中药防护作用,从而为衰老的预防和控制提供理论依据。[方法]选择72只健康Wistar雄性大鼠,随机分为空白组、模型组、西药组、中药低、中、高剂量组,每组12只,每只体重250±50g。除了空白组以外,均给0.05mg/Kg镉溶液,连续2周后停镉,同时西药组给2mL硫酸锌溶液,中药低、中、高剂量组分别给1.5g/(Kg.d)、3g/(Kg.d)、6g/(Kg.d)的冠心康颗粒,连续至60天,实验结束后结果模型组大鼠死亡2只。测定端粒酶(Telomerase)活性、丙二醛(MDA)和超氧化物歧化酶(SOD)含量。[结果]与空白组比较,各组的端粒酶活性和SOD活力明显下降,MDA含量明显增高。(P<0.05)西、中药处理组与模型组比较,端粒酶活性和SOD活力增高,MDA含量降低(P<0.05)。[结论]镉中毒可引起氧化性损伤导致促进衰老,硫酸锌和冠心康颗粒剂可以拮抗镉的毒性,提高抗氧化能力,从而可能延缓衰老。     

Chronic treatment of silymarin improves hyperalgesia and motor nerve conduction velocity in diabetic neuropathic rat

The effect of chronic silymarin (SM) treatment on hyperalgesia, sciatic motor nerve conduction velocity (MNCV) and oxidative stress in streptozotocin (STZ)‐diabetic neuropathic rat was evaluated. Rats were divided into control, diabetic, SM‐treated control and diabetic, and sodium salisylate (SS)‐treated control and diabetic. SM was administered daily at a dose of 100 mg/kg for two months. Finally, hyperalgesia and sciatic MNCV and oxidative stress markers were assessed. Diabetic rats showed a significant deficit in MNCV and markedly exhibited chemical and thermal hyperalgesia, indicating development of diabetic neuropathy. Antioxidant enzyme superoxide dismutase (SOD) level significantly reduced and malondialdehyde (MDA) level significantly increased in diabetic rats compared to control rats; SM treatment significantly ameliorated the alteration in MNCV, hyperalgesia, MDA level and antioxidant enzyme SOD in diabetic rats. These results clearly suggest the potential effect of SM in prevention and treatment of diabetic neuropathy. Copyright © 2009 John Wiley & Sons, Ltd.     

The effects of ginger supplementation on markers of inflammatory and oxidative stress: A systematic review and meta‐analysis of clinical trials

The present systematic review and meta‐analysis was conducted to investigate the effects of ginger supplementation on markers of inflammatory and oxidative stress. PubMed, Embase, Scopus, and Web of Science were systematically searched to identify relevant clinical trials evaluating the effects of ginger on serum CRP (C‐reactive protein), TNF‐α (tumor necrosis factor‐alpha), IL‐6 (interleukin‐6), PGE2 (prostaglandin E2), TAC (total antioxidant capacity), and MDA (malondialdehyde) from inception up to September 2019. Mean difference and 95% confidence intervals were pooled using a random‐effects model. Potential publication bias was assessed using visual inspection of funnel plot and Egger's weighted regression tests. After excluding irrelevant records, 20 full‐text articles that included 25 separate studies were included to the meta‐analysis. Pooled results of this study indicated a statistically significant effect of ginger on serum CRP, TNF‐α, IL‐6, TAC, and MDA levels following ginger supplementation in compared to the controls. Also, the effects of ginger on serum PGE2 was marginally significant. Moreover, the high heterogeneity was disappeared in subgroup analysis performed by age, duration, dosage, and quality. This current analysis indicates that ginger supplementation has a significant effects on serum inflammatory and oxidative stress markers.