微波固定对琥珀酸脱氢酶细胞化学影响的电镜观察

对浸泡在四种不同液体（生理盐水、０．２５％戊二醛、２．５％戊二醛、５％戊二醛）中的鼠肝组织进行微波照射固定，并按琥珀酸脱氢酶（ＳＤＨ）常规孵育、制片、电镜观察。结果表明：四个组都皮照射时间增加，液体温度不断高，肝细胞内一粒体ＳＤＨ酶活性由强膛减弱，定位趋于稳定；其中生理盐水组ＳＤＨ酶活性存最好，且以微波４０ｓ时，ＳＤＨ酶活性及定位最佳，超微结构清晰。     

应用ELISA和滴金免疫测定法检测血清肌红蛋白

从人心肌提取肌红蛋白（ｍｙｏｇｌｏｂｉｎ，Ｍｂ）抗原，制备抗Ｍｂ血清和单克隆抗体，在此基础上建立了检测血清Ｍｂ的ＥＬＩＳＡ和滴金免疫测定法（ｄｏｔｉｍｍｕｎｏｇｏｌｄｆｉｌｔｒａｔｉｏｎａｓｓａｙ，ＤＩＧＦＡ）。ＥＬＩＳＡ法检测范围２５～８００ｎｇ／ｍｌ。批内ＣＶ＜５％，批间ＣＶ＜７％。健康男、女献血员各３２例和７４例老年人，血清Ｍｂ测定结果，参考值上限分别为５５．４０、５０．６７和６５．７５ｎｇ／ｍｌ。１５例急性心肌梗塞（ＡＭＩ）患者血清Ｍｂ含量均大于１３０ｎｇ／ｍｌ．滴金免疫测定法可在５ｍｉｎ内完成检测，Ｍｂ含量＞１００ｎｇ／ｍｌ为阳性。７例ＡＭＩ患者检测结果均为阳性。两种方法结合应用对ＡＭＩ的早期诊断及疗效观察极有价值。     

散发性错配修复缺陷大肠癌的临床及分子生物学特征

目的：探讨散发性大肠癌中表现微卫星不稳定性（ｍｉｃｒｏｓａｔｅｌｌｉｔｅ ｉｎｓｔａｂｉｌｉｔｙ，ＭＳＩ）大肠癌的临床病理和分子生物学特征。方法：７９例未接受术前抗肿瘤治疗的大肠癌根治的大肠癌根治术组织收入研究，分析１４个微卫星位点，Ｋ－ｒａｓ基因和ＴＧＦβ－ＲⅡ基因异常。结果：３０／７９例（３８％）大肠癌显示不同程度ＭＳＩ。１１／７９（１４％）显示高度ＭＳＩ（≥３个位点），其中９例（８１％）显示     

~（131）I－MGb2单克隆抗体对荷胃癌裸鼠放射免疫治疗效应

本文通过对６６只荷胃癌裸鼠的实验研究探讨了１３１标记的抗胃癌单抗ＭＧｂ２对人胃癌移植物的放射免疫治疗效应．结果表明：（１）在给予９．２５ＭＢｑ／５０μｇ１３１Ｉ－ＭＧｂ２时，从给药后第二周起，肿瘤体积即明显小于对照，其肿瘤生长抑制率在给药后第三月达８４％．（２）在给予９．２５，１８．５，３７ＭＢｇ等不同剂量时，１３１Ｉ－ＭＧｂ２对肿瘤的生长抑制效应随着剂量的增加而增加，但１８．５ＭＢｑ为安全有效的治疗剂量。此外还观察了各组动物主要脏器和肿瘤组织的病理改变及１３１Ｉ—ＭＧｂ２的体内分布特点，并计算出１３１Ｉ－ＭＧｂ２在小鼠体内的累积吸收剂量。以上结果显示了１３１Ｉ－ＭＧｂ２对人胃癌放射免疫治疗的潜在价值。     

微波局部照射小鼠睾丸对精子染色体的影响

精细胞是男性遗传物质代代相传的唯一联系，了解微波辐射对生精细胞遗传物质的影响，将有益于微波的应用和防护。本实验应用２４５０ＭＨ：微波局部照射ＢＡＬＢ／ｃ小鼠睾丸，在睾九内温度达４１±０．５℃后维持１５分钟。对动物分别照射１或３次，于东次照射后二个月观察精子单倍染色体。结果显示，精子染色体的改变主要为非整倍体的增加，但和对照间无显著性差异（Ｐ＞０．０５）．该结果说明，一定剂量的微波照射，对生殖细胞的遗传物质无显著的远后效应。     

微波照射家兔睾丸抑制精子生成的有效温度阈值

利用微波的热效应原理,对实验动物睾丸进行局部照射,以提高睾丸内部温度,改变精子生成的物理环境,从而抑制精子产生,达到节育目的。本文在相同的实验照射条件,和用睾丸中心与阴囊表面同时进行对比性测温的情况下,对实验规定的不同温度组动物的实验结果进行比较,目的在于探讨微波用于节育时的有效温度阈值。实验用微波源为WL-200型医用微波理疗机。动物用体重2.5公斤以上、照前精子密度在2亿/毫升以上的雄性家兔共60只,随机分为4个大组。各组实验规定温度是以微波照射后,睾丸中心温度上升至实验额定温度为准。4个实验组照射时睾内温度分别确定为:41℃,43℃,44℃,46℃。各组动物照后定期分别进行精子密度动态观察和睾丸组织切片检查。实验结果表明,经微波一次照射20分钟后,睾丸内温度升至41℃时,受照动物精子密度并不下降,为节育无效温度。睾内温度43℃组中一半动物精子密度明显下降,但维持时间较短,节育效果不理想。睾内温度44℃组,在20分钟照射下,无论照射1次或2次,所有实验动物精子密度均有明显下降,于照射后15—19天即可降低至0.3亿/毫升以下,维持低水平时间一次照射亚组在23天左右,两次照射亚组约37天。本组在实验结束时,平均精子密度及睾丸组织学改变均能较好恢复,属于节育有效温度。睾内温度46℃组     

雀鹰舌肌的神经支配

鸟类的鸣叫发自气管与支气管交界处的特殊装置一鸣管。但需要舌的配合才能发出变化多样的鸣声。本文研究雀鹰（Ａｃｃｉｐｉｔｅｒ ｎｉｓｕｓ）舌肌的神经支配。１材料和方法用成年雀鹰５只,体重１７０～２５０克,雌雄不拘。经２５％乌拉坦腹腔麻醉（ｉｇ／ｋｇ）,将ＣＢ－ＨＲＰ溶液（中国医学科学院提供）０,５μl分别注入一侧舌失或舌体,动物存活 ４８ ｈ,麻醉后经颈动脉相继灌注;（１）生理盐水２００ ｍｌ;（２）固定液（１％多聚甲醛,１．２５％戊二醛,０． ｌｍｏｌ／Ｌ磷酸缓冲液）２００～２５０ ｍｌ;（３）１０％蔗…     

2450MHz微波辐射对离体培养人肝癌细胞系(BEL—7402)的生物效应

本实验应用2450MHz微波进行了对体外培养人肝癌细胞系生物效应的研究。采用一循环液体散热装置控制介质温度,观察了在一定照射条件下对BEL-7402细胞系形态和增殖的影响。 在实验中观察到,50mW/cm~2～100mW/cm~2连续波一次照射30分钟对细胞的损伤程度与功率密度呈平行关系。其中75mW/cm~2照射30分钟,介质温度维持在43±0.57℃细胞生长抑制率为41％～65％,对细胞分裂和DNA合成均有明显的抑制作用,但这一作用在解除微波照射48小时后逐渐恢复。     

细胞粘附分子在狼疮性肾炎及膜增殖性肾炎肾组织中的表达

用免疫组化方法和计算机图像分析系统，对２５例狼疮性肾炎（ＬＮ）ＩＶ型及１５例膜增殖性肾炎（ＭＰＧＮ）Ｉ型患者肾组织内ＩＣＡＭ－１和ＶＣＡＭ－１的表达进行了定量研究。结果显示：ＭＰＧＮ及ＬＮ患者肾组织中ＩＣＡＭ－１及ＶＣＡＭ－１表达均显著增加，ＬＮ患者ＩｃＡＭ－１在肾小球内皮细胞表达最强，且与内皮细胞增殖程度显著正相关。     

热源植入物规则排列组织的温度分布:分析估计〔英〕

热源植入物规则排列组织的温度分布：分析估计〔英〕／ＨａｉｄｅｒＳＡ…／／　ＩＥＥＥＴｒａｎｓＢＭＥ．－１９９３，４０（５）．－４０８我们在生物热传递等式的基础上取得厂近似分析模型，可用来计算规则分布于电介表层热源植入物（例热水试管、电热棒或感应热铁磁...     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应

背景：局部注射具有成骨作用的辛伐他汀,可显著增加骨质疏松大鼠股骨颈及股骨髁部的骨密度及力学强度,分析局部注射辛伐他汀对股骨髁骨小梁的影响。 目的：进一步研究骨质疏松大鼠股骨内局部注射辛伐他汀对股骨髁骨小梁的影响。为将辛伐他汀应用于临床骨质疏松局部治疗提供实验基础。 方法：18只雌性SD大鼠双侧卵巢切除后3个月,制备大鼠骨质疏松模型。实验大鼠随机数字表法均分为3组,分别在实验大鼠的右侧股骨髓腔内单次注射辛伐他汀溶液5 mg、10 mg,对照组单纯注射空白载体。分别在注射后1个月处死大鼠并取材。Micro-CT扫描并定量分析骨组织形态变化。 结果与结论：给药后1个月,Micro-CT扫描结果显示,辛伐他汀治疗组的骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组。说明疏松骨骼单次注射小剂量辛伐他汀可显著促进股骨髁部骨小梁改建,改善骨骼微结构,可为强化局部、防治骨质疏松骨折的新选择进一步提供实验基础。     

石斛属民族药用植物的分类及生药学研究

中药石斛早在《神农本草经》中就被列为上品,其应用历史悠久,具有养阴生津、补肾益气、润喉护嗓、活血明目、抗癌防老等功效。本文对国内外石斛属民族药用植物的分类及生药学研究进行了综述。     

膝关节骨性关节炎的关节镜治疗体会

目的我科自2001～2005年4月对59例67膝的骨性关节炎（0A），进行关节镜检查及镜下清理术。方法镜检：膝关节骨性关节炎伴不同程度滑膜炎，滑膜皱壁粘连纤维片，关节软骨不同程度破坏。镜下清除增生滑膜，松解粘连，去除剥脱软骨、修复关节面。结果随访2月～4年，平均1.5年，术后综合评估2月～1年，优良率94％；1～2年，优良率79.2％；2年以上优良率54.8％。结论关节镜对膝关节骨性关节炎诊断能提供了比较全面的资料，并对骨性关节炎早期有良好疗效，具有创伤小、恢复快、并发症少和重复治疗等优点。     

Anatomical study of distribution of valves of the cutaneous veins of adult''s limbs

Since the cutaneous veins of the four limbs have been used as autogenous grafts in the treatment of cardiovascular disorders, knowledge of distribution of the valves is increasingly required depending upon the use. In the gross anatomical study of distribution of valves of the trunci venae, there has been argument about locational relationships between the venous roots and the valves in the vicinity of the roots and the inter-valvular distance. However, there have been only few reports discussing detailed information about valves of the cutaneous veins of the four limbs. The authors observed patterns of distribution of the cutaneous venous valves of the four limbs of cadavers prepared for practice in anatomy. The following parts were excised from each cadaver: the cephalic, basilic, and the great saphenous veins, which originate from the acral venous network and flow into the proximal deep veins, and the venous roots communicating with these veins. An incision was made on each excised vein in the direction of the long axis under observation with a stereoscopic microscope, and the inter-valvular distance and the distance between the valve and the orifice of venous root in the vicinity of the valve were measured. The inter-valvular distance varied with type of the truncus venae, and it varied according to area even in the same truncus venae.     

Pathways of the realization of immunomodulating effects of neurotransmitters

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 401–404, April, 1993     

Characterization of sterols of three digenetic trematodes of buffalo

Sterols of three digenetic trematodes were isolated and characterized by infrared and 1H nuclear magnetic resonance spectroscopies, and gas-liquid chromatography and gas chromatography-mass spectrometry. Sterols identified were cholesterol, cholestanol, 24-methylcholesterol, 24-methylcholestanol, 24-ethyl-22-dehydrocholesterol, 24-ethyl-22-dehydrocholestanol, 24-ethylcholesterol and 24-ethylcholestanol.     

Mechanisms of generation of slow components of bioelectric activity in diagnostics of functional state of the gastrointestinal tract

New electrophysiologic devices were used for the diagnostics of the state of excitable structures in the gastrointestinal tract and correction of their motor functions. Bioelectrical and biomechanical activities form the basis of functioning of internal organs. The mechanisms of generation of slow bioelectrical activity that are important for clinical and physiological studies are described. One of these mechanisms is a capacitance parametric transducer converting the energy of contractions into specific electric signals reflecting muscle functions. Another mechanism results from slow oscillations of resting potentials of interrelated excitable cells in large neuromuscular structures of internal organs. The elaborated procedure is efficient for preventing early postoperative paresis of the gastrointestinal tract. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 10, pp. 448–452, October, 1999     

胃小弯侧血管弓的MDCT解剖

目的：研究正常胃小弯侧血管弓及其属支血管的多排螺旋CT表现。方法：回顾性分析51例正常成人的上腹部CT资料,观察胃小弯侧血管弓及其属支血管的显示情况、血管弓形态及其与胃壁的关系。结果：51例均显示胃小弯侧血管弓,血管弓贲门部属支、胃体部属支和幽门部属支分别显示46例、15例和10例;血管弓与胃壁紧邻41例。结论：MDCT检查可良好显示正常胃小弯侧血管弓及其属支血管。     

Assessment of the efficacy of treatment of hemangiomas

Assessment of the aftereffects of cryoexposure and ultrahigh-frequency cryoexposure on hemangioma tissue of various types, cavernous and squamous, showed a higher cryogenic effect in hemangiomatous tissue preexposed to ultrahigh-frequency waves. A quantitative criterion is proposed for assessing the efficacy of the studied methods of exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 669–672, June, 1995