内源性高甘油三酯血症患者血浆VLDL、LDL及HDL对血小板聚集功能的影响

目的　研究内源性高甘油三酯血症 (HTG)患者血浆极低密度脂蛋白 (VL DL )、低密度脂蛋白 (L DL )及高密度脂蛋白 (HDL )对血小板聚集功能的影响。方法　对 2 1例内源性高甘油三酯血症患者与 2 1例正常对照者用一次性密度梯度超速离心法分离血浆 VL DL、L DL及 HDL。测定这三种脂蛋白的 2 34nm吸光度 (A2 34 )、电泳迁移率 (REM)及硫代巴比妥酸反应物质 (TBARS)含量。分别将这三种脂蛋白加入由正常人新鲜血浆构成的反应系统中 ,用血小板聚集仪分别测定 ADP诱导的血小板 5 min最大聚集率。结果　内源性 HTG患者血浆 TG含量平均升高 2 .73倍 ,HDL - C下降 1.71倍 ,同时 L PO升高 1.2 2倍 ;HTG组 VL DL、L DL及 HDL的 REM、A2 34 、TBARS含量均较对照组显著增加 (P<0 .0 1) ,表明内源性 HTG患者血浆 VL DL、L DL及 HDL均发生了氧化修饰生成Ox- VL DL、Ox- L DL及 Ox- HDL。血小板聚集率在分别加入 HTG组患者的 VL DL、L DL及 HDL后均比加入正常组相应脂蛋白明显增加 (P<0 .0 5及 P<0 .0 1)。相关分析表明 ,HTG- L DL及 HTG- HDL A2 34 、REM及 TBARS含量与血小板聚集率呈正相关 (P<0 .0 1)。结论　 HTG患者血浆 VL DL、L DL及 HDL发生了氧化修饰 ,并使血小板聚集增加     

高淀粉膳食诱导高甘油三酯血症大鼠血浆及肝脏磷脂含量变化的研究

目的 研究高淀粉膳食诱导高甘油三酯血症(HTG)大鼠血浆及肝脏磷脂 (PL) 含量的变化.方法 实验组大鼠喂饲高淀粉膳食,6 d后氧化酶法测定血浆甘油三酯(TG)、胆固醇(TC)、血糖,抗坏血酸还原法测定PL含量.磷钨酸沉淀法分离高密度脂蛋白(HDL),测定HDL-TG、HDL-C、HDL-PL,计算NHDL-TG、NHDL-C和NHDL-PL浓度.结果 实验组大鼠较普通膳食喂饲的对照组血糖、TG、NHDL-TG、PL升高(P＜0.05),血浆HDL-C、HDL-PL浓度和HDL-PL/HDL-C比值降低(P＜0.05).实验组大鼠肝脏TG、PL含量升高(P＜0.05).相关性分析发现,实验组血浆PL与TG呈正相关,与HDL-PL呈负相关,与NHDL-TG正相关性较对照组增强(P＜0.05);实验组HDL-PL与血糖、血浆PL、肝脏PL呈负相关,与TG 、NHDL-TG负相关性较对照组增强(P＜0.05);实验组肝脏PL与肝脏TG相关性较对照组增强(P＜0.05).结论 高淀粉膳食诱导HTG模型大鼠伴血浆及肝脏PL含量增加,HDL中PL含量减少,血浆及肝脏PL含量与其他脂质含量的相关性状态也发生改变.     

142例冠心病患者血脂及载脂蛋白AI,B100,CI,CII及CIII的研究

Serum triglycerides (TG), cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and apolipoproteins AI, B100, CI, CII and CIII were analyzed in 142 patients with coronary heart disease (CHD) and 64 age-matched healthy subjects in the Chengdu area. Significantly increased TG and decreased HDL-C were found in the CHD patients (P less than 0.001). Serum apo AI and CII were significantly decreased in the CHD patients compared with the normal subjects (P less than 0.01 or P less than 0.001). Serum apo B100 and CIII were slightly higher in the CHD group than in the normal group (P less than 0.001 or P less than 0.05). No significant differences were observed in apo CI levels in the CHD patients when compared with the normal subjects. The results were discussed.     

内源性高甘油三酯血症患者血浆高密度脂蛋白亚类分析

内源性高甘油三酯血症常伴血浆ＨＤＬ－Ｃ含量下降及ＨＤＬ组成异常。为了研究ＨＴＧ患者血浆ＨＤＬ亚类的改变,作者建立了密度梯度聚丙烯酰胺梯度胶电泳法,对２５例ＨＴＧ患者及２５例年龄、性别匹配的血脂正常者。     

高甘油三酯血症中对氧磷酯酶-1活性的研究

目的:观察高甘油三酯血症(HTG)状态下高密度脂蛋白(HDL)相关抗氧化酶——对氧磷酯酶-1(PON-1)的活性变化。方法:收集湖北武汉地区40例单纯性HTG患者和65例正常人群的血样,比较血脂分布特征,利用酶水解底物对氧磷的原理测定血浆HDL特征性PON-1的活性;同时以脂蛋白脂肪酶基因敲除(LPL-/-)鼠为HTG动物模型,检测血清中甘油三酯(TG)、总胆固醇(TC)含量和PON-1的活性改变。结果:①HTG组与对照组相比,血浆载脂蛋白(apo)B100水平显著升高(P<0.01),HDL-C、apoA-Ⅰ水平降低(P<0.05);PON-1酶活性显著降低(P<0.001);②HTG合并正常HDL-C组与低HDL-C对照组比较,PON-1活性无明显差别,但PON-1/apoA-Ⅰ比值显著降低(P<0.05);③LPL-/-鼠与对照组比较TG明显升高(P<0.001),PON-1活性显著下降(P<0.001)。结论:血清PON-1活性降低反映HDL抗氧化功能的异常,这可能与HTG易致动脉粥样硬化的发生密切相关。     

大鼠肝实质及非实质细胞VLDL和HDL受体的研究

Saturable high-affinity VLDL and HDL receptor on parenchymal cells (PC), and non-parenchymal cells (NPC) freshly isolated from rat liver were studied. The VLDL- and HDL-receptor could mediate liver PC and NPC to bind, uptake, and degrade 125I-labeled human VLDL and apoE-deficient HDL3, and the activities of these two receptors (expressed as ng/mg cell protein) on NPC were about 10- and 4-fold higher than those on PC, respectively. VLDL receptor on NPC with kd 15.0-34.2 micrograms/ml and Bmax 2170-2607 ng/mg cell protein could be inhibited by EDTA, and down-regulated by cell cholesterol content. HDL receptor on NPC with kd 10.1-17.7 micrograms/ml and Bmax 1004-2738 ng/mg cell protein could not be inhibited by EDTA, but could be up-regulated by cell cholesterol content. Competitive inhibition assay showed that VLDL receptor could not only bind VLDL and LDL, but also bind HDL3 to some extent. Unlabeled purified apolipoprotein CIII-1, but apoAI, CI, CII, could effectively inhibit 125I-labeled VLDL binding to NPC. These results suggest that liver NPC may be more active than PC in clearing VLDL and HDL from circulation, and apolipoprotein CIII play an important inhibitory role in these receptor-mediated processes.     

血脂水平对无症状高血压患者ST-T改变的影响

目的 探讨无症状高血压患者ST-T改变的影响因素。方法 以无症状高血压患者为研究对象,按心电图表现分为ST-T改变组(28例)和无ST-T改变组(20例),检测两组患者的血糖、血脂水平。结果 与无ST-T改变组相比,ST-T改变者血清总胆固醇水平明显增高,而高密度脂蛋白水平则明显降低(P<0.05),其他检测指标在两组无显著性差异。结论 在血脂正常的无症状高血压患者中,相对较高的总胆固醇水平促使或加重心肌缺血,而高密度胆固醇对心肌损害起着保护作用。     

血脂水平对无症状高血压患者ST—T改变的影响

OBJECTIVE: To identify the factors affecting ST-T segment in asymptomatic hypertensive patients. METHODS: The asymptomatic patients with hypertension were divided into abnormal ST-T group (n=28) and normal ST-T group (n=20), in whom the serum levels of glucose, total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), very-low-density lipoprotein cholesterol (VLDL) were measured. RESULTS: Compared with patients with normal ST-T segment, patients with abnormal ST-T segment had significantly higher serum levels of TC, but significantly lower HDL. The serum levels of glucose, TG, LDL, VLDL differed little between the 2 groups of patients. CONCLUSION: In asymptomatic patients with hypertension, higher serum TC level may cause or worsen ischemic myocardial damage, for which HDL plays a protective role.     

严重高三酰甘油血症对小鼠生育及其后代糖脂代谢的影响

目的 严重高三酰甘油血症（hypertriglyceridemia,HTG）伴发于妊娠可能诱发急性胰腺炎,不良妊娠结局明显增高,但关于HTG对后代的远期影响如糖脂代谢的影响未见报道.本研究旨在探讨载脂蛋白CIII（apolipoprotein CIII,Apo CIII）转基因严重HTG的雌性小鼠对后代糖脂代谢的影响.方法应用Apo CIII转基因的严重HTG雌性及正常雌性小鼠分别与正常雄性小鼠交配,观察严重HTG雌性小鼠生育能力,并得到3组后代：母系为转基因的正常（maternal control,MC）和转基因后代（maternal transgenic,MT）及正常对照组（normal control,NC）.比较了上述3组小鼠出生体质量及生长速度、血浆三酰甘油（triglycerides,TG）、总胆固醇 （total cholesterol,TC）、糖代谢的差异.结果 Apo CIII转基因导致的严重HTG对雌性小鼠生育能力无影响,Apo CIII转基因的雌性小鼠所生育后代小鼠出生体质量及生长速度均与对照组差异常无统计学意义,MT组小鼠血浆TG及TC浓度于出生14及28 d均较MC及NC组显著升高（P〈0.01）,并出现轻度糖耐量受损,空腹胰岛素（fasting insulin,FINS）水平及稳态模型评估胰岛素抵抗指数（homeostasis model assessment-insulin resistant index,HOMA-IR）也较其他2组增高（P〈0.05）.结论Apo CIII转基因严重HTG的雌性小鼠可正常妊娠、分娩及哺乳.严重HTG的Apo CIII转基因小鼠可以通过母系遗传而导致转基因后代发生糖耐量受损,高胰岛素血症及胰岛素抵抗指数增高.