毛细管电泳技术在核酸分析中的应用

毛细管电泳技术以其高效、快速、所需样品少等特点而广泛应用于核酸的分析。本文综述了近5年来毛细管电泳技术在核酸定性、定量和筛选研究中的应用,包括反义寡核苷酸的分析,适配体的筛选,基因突变的分析,此外对微芯片毛细管电泳技术及其在核酸分析研究中的应用进行了介绍,并对其前景进行了展望。     

高效毛细管电泳法在体内药物分析的应用

目的:介绍高效毛细管电泳技术近年来在体内药物分析领域的应用情况。方法:查阅相关文献,进行综合、分析和归纳。结果:高效毛细管电泳技术在体内化学药物、滥用药物、抗菌药物、生化药物和对映体药物分析中得到了广泛的应用。结论:高效毛细管电泳技术具有高效、快速、分离模式多等特点,在体内药物分析中具有广阔的应用前景。     

亲和毛细管电泳及其在测定药物-蛋白质结合常数中的应用

药物与蛋白质的结合会影响药物在体内的过程及活性。平衡透析法和超滤法是测定药物—蛋白质结合常数的传统方法，但各有不足。近年来，随着毛细管电泳技术的不断发展，亲和毛细管电泳(ACE)以其独特的优势，已被用于测定药物—蛋白质结合常数。本文阐述ACE定义、分类、原理及其在测定药物—蛋白质结合常数中的应用。     

电泳技术在药物分析中的应用

本文概述了电泳技术产生的背景以及发展历程,简要介绍了几种常用的电泳技术的原理、特点以及在药物分析中的应用,尤其是高效毛细管电泳法在许多科学试验中被广泛应用并取得了卓越的成效.     

毛细管电泳技术在黄酮类化合物分析中的应用进展

综述植物药和中药中黄酮类化合物的电迁移分析方法,对毛细管电泳技术的分离原理进行简要的阐述,列举2005～2011年部分黄酮化合物的毛细管电泳分析实例.天然药物黄酮化合物毛细管电泳技术主要有毛细管区带电泳、胶束电动毛细管电泳和毛细管电色谱3种分离模式.     

高效毛细管电泳技术在药物分析中的应用

高效毛细管电泳技术(HPCE)是80年代以来迅速发展的一种新型分离分析方法,具有高灵敏度、高分辨率、高速度、样品用量低、成本低的优点[1],目前已广泛应用于生物工程、医学临床检验、法庭科学、药物分析等领域.本文重点介绍它在药物分析方面的应用.     

毛细管电泳技术在研究生物分子间相互作用和药物筛选中的应用

毛细管电泳技术是八十年代初发展起来的一种新型分离分析技术。由于其分析时间短，样品消耗少，分离度高，应用灵活，能够在生理条件或者接近生理条件的缓冲液中运行等优点，广泛应用于研究生物分子间的相互作用。本文就毛细管电泳研究生物分子间相互作用的基本原理，及其在生物分子间相互作用和药物筛选中的应用进行了综述。对不同的分子间相互作用体系，如蛋白质-蛋白质／多肽，蛋白质-DNA／RNA，蛋白质-糖，抗原-抗体，蛋白质-药物，脂质体-蛋白质／药物等进行了论述。同时也探讨了毛细管电泳从组合化学库中筛选先导药物及其在药物筛选中的应用。     

高效毛细管电泳在临床药理中的应用

目的：总结近年来高效毛细管电泳技术在临床药理学中的应用。方法：从高效毛细管电泳的类型、特点、原理及在治疗药物监测及样品分析等方面进行综述。结果：介绍了该项技术在治疗药物监测和样品分析中的优势、前景及诊断治疗方面的价值。结论：该项技术值得在临床药理工作中大力推广。     

芯片电泳的发展及其在医药学中的应用

介绍近几年来兴起的芯片电泳技术,以及其在医药学方面的应用。芯片电泳是基于传统毛细管电泳发展起来的一种新型的微全分析系统,具有高速、高效、需样品量极小、低耗等特点,在药物、DNA、蛋白质等复杂样品的分离分析方面有巨大潜力。     

毛细管电泳在中药研究中的应用

简要的综述了毛细管电泳技术在中药研究的各个方面的应用.     

Capillary Electrophoresis in Pharmaceutical Analysis

Capillary electrophoresis (CE) is a separation technique particularly suited to the analysis of pharmaceutical compounds. This review offers a detailed discussion of the four common modes of detection coupled to CE—UV absorption, fluorescence, electrochemical, and mass spectrometry—and gives examples of the use of these methods in pharmaceutical analyses. Sample preparation and pretreatment techniques used for CE separations are described, as well as methods of preconcentration including hydrophobic retention, affinity concentration, sample stacking, and isotachophoresis. The use of affinity CE, chiral CE, and capillary gel electrophoresis for analysis of pharmaceuticals is covered in detail, and recent advances in capillary electrochromatography and CE on a chip are also discussed.     

毛细管电泳法和分光光度法测定有机氮源中可溶性无机磷的比较

通过优选缓冲液体系，建立了阴离子的毛细管电泳（CE）检测法，以测定天然有机氮源中可溶性无机磷的含量，并与分光光度法进行了比较。结果显示，分光光度法检测限较低，但CE法线性范围更广，结果准确，在自动化快速分析方面亦有较大优势，更适于微生物培养过程中离子含量的动态分析。     

Enantiomeric separation of antimalarial drugs by capillary electrophoresis using neutral and negatively charged cyclodextrins

Capillary electrophoresis (CE) methods for chiral resolution of five antimalarial drugs (primaquine, tafenoquine, mefloquine, chloroquine and quinacrine) were developed by using a wide selection of neutral and anionic cyclodextrin (CD) derivatives. The use of sulfobutyl-β-CD and carboxymethyl-β-CD (CMBCD) resulted in good resolution of quinacrine and tafenoquine, respectively. New results are presented for resolutions of chloroquine and mefloquine. Application of carboxyalkyl- and sulfobutyl-CD derivatives provided improved resolution for primaquine. The impurity in primaquine sample detected by CE was identified as quinocide by MS and NMR. CMBCD provided not only the best separation of primaquine from quinocide but also the simultaneous complete resolution of both compounds.     

The Electrophoretic Mobility of Tripeptides as a Function of pH and Ionic Strength: Comparison with lontophoretic Flux Data

Capillary electrophoresis (CE) is an extremely efficient separations tool which can also be used to determine fundamental molecular parameters, e.g., the electrophoretic mobility of a molecule. We have studied the changes in the CE estimated electrophoretic mobility of thyrotropin releasing hormone (TRH) as a function of pH and ionic strength. Further, we have used CE to estimate the mobilities of two synthetic analogs of TRH to examine the behavior of positive (basic) and negative (acidic) peptides under the conditions of this work. These data were then compared with literature values of iontophoretic flux of these molecules under similar formulation conditions. Our results suggest that CE could potentially assist formulation optimization for the iontophoretic delivery of peptides.     

Simultaneous separation and confirmation of amphetamine and related drugs in equine plasma by non-aqueous capillary-electrophoresis-tandem mass spectrometry

A non-aqueous capillary electrophoresis-mass spectrometry (NACE-MS) method was developed for simultaneous separation and identification of 12 amphetamine and related compounds in equine plasma. Analytes were recovered from plasma by liquid-liquid extraction using methyl tertiary butyl ether (MTBE). A bare fused-silica capillary was used for separation of the analytes. Addition of sheath liquid to the capillary effluent allowed the detection of the analytes by positive electrospray ionization mass spectrometry using full scan data acquisition. The limit of detection (LOD) for the target analytes was 10-200 ng/mL and that of confirmation (LOC) was 50-1000 ng/mL in equine plasma. Capillary electrophoresis (CE) and mass spectrometry (MS) parameters were optimized for full CE separation and MS detection of the analytes. Separation buffer comprised 25 mM ammonium formate in acetonitrile/methanol (20: 80, v/v) plus 1 M formic acid. Sheath liquid was isopropanol-water-formic acid (50:50:0.5, v/v/v). Samples were hydrodynamically injected and separated at 25 kV. Analytes were electrokinetically separated and mass spectrometrically identified and confirmed. This simple, fast, inexpensive and reproducible method was successfully applied to post race equine plasma and research samples in screening for amphetamine and related drugs.     

Liquid chromatography and capillary electrophoresis analysis of polyanionic quinobene

Quinobene is the tetrasodium salt of an organic tetrasulphonic acid. Its unusual solubility characteristics makes the development of LC analysis difficult. However, a specific, precise and accurate LC assay was eventually achieved for quinobene. The assay required gradient elution and was not efficient for quinobene with respect to plate number. As an alternative, a capillary electrophoresis (CE) assay was also developed for quinobene. The CE assay was comparable to the LC assay in precision and accuracy. It was unaffected by the unusual solubility characteristics of quinobene and was more specific, efficient and rugged than the LC assay.     

2-甲基-β-环糊精作为手性选择剂对5种药物对映体的毛细管电泳拆分研究

目的以2-甲基-β-环糊精(2-O-methylated-β-cyclodextrin,2-O-M-β-CD)为手性添加剂,利用毛细管电泳法(capillary electrophoresis,CE)对消旋体药物甲溴后马托品、氧氟沙星、酮康唑、利阿唑和卡维地洛进行拆分研究。方法考察了背景电解质溶液的pH值、2-O-M-β-CD浓度、缓冲盐浓度对分离的影响,优化了分离条件。结果在最佳分离条件下,甲溴后马托品、氧氟沙星、酮康唑、利阿唑对映体达到完全分离,分离度分别为3.6、2.8、1.7、1.5;卡维地洛对映体分离度达到1.4。结论所建立的毛细管电泳法适于除卡维地洛外其余4种药物的对映体分离。     

小檗碱与大黄酸沉淀作用的毛细管电泳法

目的研究大黄酸和小檗碱沉淀反应的热力学和动力学性质，为深入研究中药复方泻心汤和芍药汤提供依据。方法用毛细管电泳法测定在不同条件下沉淀反应溶液中两组分的平衡浓度，研究沉淀反应的热力学和动力学。结果沉淀中两组分摩尔比1∶1，20 ℃时溶度积常数k_sp=［B］［R］=(3.29±0.19)×10^-9mol²·L^-2。沉淀反应为吸热过程，k_sp受温度的影响不大。沉淀反应在混合时很快发生，然后是沉淀的陈化过程。结论中药复方泻心汤和芍药汤在煎煮过程中产生的来自于大黄蒽醌和生物碱的沉淀有一定的溶解度。毛细管电泳采用加大进样量后用峰高定量的方法有助于提高简单样品测定结果的重现性。     

毛细管电泳法测定黄酮类化合物(英文)

黄酮类化合物由于其在生物学及生理学中的重要作用受到了广泛的关注。本文综述了毛细管电泳法测定黄酮类化合物的应用,包括手性分离以及毛细管电泳-质谱联用技术。此外,还讨论了提高毛细管电泳法灵敏度的技术,如样品堆积法、扫集法和等速电泳法等。