丙型肝炎患者血清IL-1α、IL-6、TNF-α、IFN-γ的检测及意义

为探讨丙型肝炎血清中ＩＬ－１α、ＩＬ－６、ＴＮＦ－α、ＩＦＮ－γ的含量及与临床关系,ＥＬＩＳＡ法对３３例各丙肝患者检测血清中ＩＬ－１α、ＩＬ－６、ＴＮＦα并与健康人群对照,结果发现,丙型肝炎临床各型患者血清中ＩＬ－１α、ＩＬ－６、ＴＮＦ－α与患者病情轻重呈正相关,慢性丙肝ＩＬ－６含量高;ＩＦＮ－γ氏,ＩＬ－１α与ＩＬ－６呈正相关,认为ＩＬ－１α、ＩＬ－６、ＴＮＦ－α、ＩＦＮ－γ对丙肝患者病情观察和     

Expression of ICAM-1, HLA-DR, and CD80 on peripheral circulating CD_1αDCs induced in vivo by IFN-αin patients with chronic hepatitis B

AIM: To explore the effects of interferon-α(IFN-α) application on peripheral circulating CD1αdendritic cells (DCs) in patients with chronic hepatitis B, and the expression of HLA-DR, CD80, and ICAM-1 on CD1αDCs in order to explore the mechanism of immune modulation of IFN-α. METHODS: By flow cytometry technique, changes of CD1αDCs were monitored in 22 patients with chronic hepatitis B treated with IFN-αand in 16 such patients not treated with IFN-αwithin three months. Meanwhile, the expression of HLA-DR, CD80, and ICAM-1 on CD1αDCs was detected. RESULTS: In the group of IFN-αtreatment, the percentage of CD1αDCs in peripheral blood mono-nuclear cells was increased after three months of therapy. In patients who became negative for HBV-DNA after IFN-αtreatment, the increase of DCs was more prominent, while in control, these changes were not observed. Increased expression of HLA-DR, CD80, and ICAM-1 on CD1αDCs was also observed. CONCLUSION: CD1αDCs can be induced by IFN-αin vivo, and the immune related molecules such as HLA-DR, CD80, and ICAM-1 are up-regulated to some degree. This might be an important immune related mechanism of IFN-αtreatment for chronic hepatitis B.     

The Correlation between TNF-α, IFN-γ, IL-8 and Coronary Heart Disease

Objectives To elucidate the potential role of cytokines in the pathogenesis of coronary heart disease (CHD). Methods TNF-α and IFN-γ activity , IL-8 levels of plasma and supernatants were measured in 62 patients with CHD and 30 health controls by methods of direct cytotoxicity assay, cytopathic effect inhibition test and ELISA respectively.Results Both TNF-α activity and IL-8 levels of plasma in CHD patients were higher and IFN-γ activity of supernatants in CHD patients were lower than those of healthy controls ( P ＜ 0. 001 ) , There have significant differences between healthy controls and the subgroups of CHD ( P ＜ 0.01 ). IL-8 levels of plasma increased with the advancing of the disease and there have obvious differences among subgroups of the illness ( P ＜ 0.05 ).TNF-α activity of plasma in stable angina pectoris ( SAP ) subgroup was lower than those of unstable angina pectoris ( UAP )and acute myocardial infarction (AMI) subgroups, the differences between SAP and UAP or AMI were significant ( P ＜ 0.05 ), But there have no significant differences between UAP and AMI ( P ＞ 0.05 ). However, IFN-γ activity of supernatants showed no difference among any subgroups. Conclusions there have close relations between TNF-α,IFN-γ,IL-8 and CHD.     

病毒性肝炎患者血清TNF—α,IL—6和IL—8水平动态研究

病毒性肝炎病理机制十分复杂，细胞因子在其中的作用越来越受到重视，我们对３８９例各型病毒性肝炎患者进行了血清ＴＮＦα、ＩＬ６和ＩＬ８水平检测和动态变化的研究，以探讨其在病毒性肝炎发病中的作用。材料与方法一、研究对象从泰山医学院附属医院、山东省泰安...     

IL-1β, IL-6, IL-8, IL-10, IFN-γ, TNF-α and its relationship with lipid parameters in patients with major depression

There is some evidence that an immune response with an increased production of proinflammatory cytokines frequently accompanies major depression. The aim of this study was to determine the serum levels of interleukines (IL-1??, IL-6, IL-8, IL-10), tumor necrosis factor-alpha (TNF-??), interferon-gamma (IFN-??) and immonuglobulines (IgG, IgA and IgM) levels and to examine the relationships between all above parameters and lipid parameters. The study group included 30 patients and 30 healthy volunteers. Although total cholesterol, HDL-cholesterol, and IgM levels were increased significantly (p?<?0.05) in patients and compared to those of the controls, no statistically significant differences (p?>?0.05) were observed with other parameters. IFN-?? were positively correlated with total cholesterol (r?=?0.425; P?=?0.019) and LDL-cholesterol (r?=?0.391; P?=?0.032) levels in patients. Other cytokines and immunoglobulins did not show any correlation with lipid parameters. It was concluded that although no differences was observed in cytokines and immunoglobulin levels in the present study, the dysregulation of the lipids and immune system including the cytokine network is associated with the etiology and pathophysiology of major depressive disorders.     

The role of endotoxin,TNF—α,and IL—6 in inducing the state of growth hormone insensitivity

AIM：Critical illnesses such as sepsis,trauma,and burns cause a growth hormone insensitivity,wehich leads to an increased negative nitrogen balance.Endotoxin is generously released into blood under these conditions and stimulates the production of proinflammatory cytokines such as TNF-α,IL-6,and IL-1 which may play a very important role in inducing the growth hormone insensitivity,The objective of this current study was to investigate the role of endotoxin,TNFα and IL-6 in inducing the growth hormone insensityvity at the receptor and post-receptor levels.METHODS:Spageu-Dawley rats were injected with endotoxin,TNF-α,and IL-6,respectively and part of rats injected with endotoxin was treated with exogenous somatotropin simultaneously,All rats were killed at different time points,The expression of IGF-I,GHR,SOCS-3 and β-actin mRNA in the liver was detected by RT-PCR and the GH levels were measured by radioimmunoassay,the levels of TNF-α and IL-6 were detected by ELISA.RESULTS:There was no significant difference in serous GH levels between experimental group and control rats after endotoxin injection,However,liver IGF-1 mRNA expression had been obviously down-regulated in endotoxeminc rats.Liver GHR mRNA expression also had a predominant downregulation after endotoxin injection,The lowest regulation of liver IGF-I mRNA expression occurred at 12h after LPS injection,being decreased by 53% compared with control rats.For GHR mRNA expression,the lowest expression occurred at 8h and had a 81% decrease.Although SOCS-3 mRNA was weakly expressed in control rats,it was strongly up-regulated after LPS injection and had a 7.84 times increase compared with control rats.Exogenous GH could enhance IGF-1 mRNA expression in control rats,but if did fail to prevent the decline in IGF-1 mRNA expression in endotoxemic rats,Endotoxin stimulated the production of TNF-α and IL-6,and the elevated IL-6 levels was shown a positive correlation with increased SOCS-3 mRNA expression.The liver GHR mRNA expression was obviously down-regulated after TNF-α iv injection and had a 40 decresase at 8 h,but the liver socs-3 mRNA expression was the 4.94 times up-regulation occurred at 40 min after IL-6 injection.CONCLUSION:The growth homone insenstivity could be induced by LPS injection,which was associated with down regulated GHR mRNA expression at receptor level and with up-regulated SOCS-3 mRNA expression at post-receptor level The in vivo biological activities of LPS were mediated by TNF-α and IL-6 indirectly,and TNF-αand IL-6 may exert their effects on.the receptor and post-receptor levels respectively.     

Graves' disease and gene polymorphism of TNF-α, IL-2, IL-6, IL-12, and IFN-γ

The role of genetic factors in the pathogenesis of Graves' disease (GD) is not clear. The purpose of this study was to investigate the association between single nucleotide polymorphisms in pro-inflammatory cytokine genes and GD in Iranian patients. A case-control hospital-based study was carried out on 107 GD patients and 140 healthy controls. Cytokine typing was performed by polymerase chain reaction with sequence-specific primers (PCR-SSP) assay. The allele and genotype frequencies of the following cytokine genes were determined: TNF-α (-308A/G, -238A/G), IL-2 (-330T/G, +166G/T), IL-6 (-174C/G, A/G nt565), IL-12 (-1188A/C), and IFN-γ (UTR 5644A/T). The following alleles and genotypes were significantly overrepresented in patients: TNF-α -308A allele (P < 0.01) and AA genotype (P < 0.05), IL-2 -330G allele (P < 0.01) and GG genotype (P < 0.01), IL-6 -174C allele (P < 0.01) and CC genotype (P < 0.01), IL-12 -1188C allele (P < 0.01) and CC genotype (P < 0.01), IFN-γ UTR5644T allele (P < 0.01) and TT genotype (P < 0.01). In conclusion, this is the first study to show a significant association between GD and IL-2 -330G, IL-12 -1188C, and IFN-γ UTR 5644T alleles. Our results support the hypothesis that polymorphism in pro-inflammatory cytokines might be involved in predisposition to GD.     

IL-10, TNF-α and IFN-γ levels in serum and stomach mucosa of Helicobacter pylori-infected patients

H. pylori is a human pathogen that colonizes the epithelium of the stomach. The host immune response may influence the disease process, where cytokines play important roles in the development of disease. In this study, the concentrations of selected cytokines in the gastric antrum and stomach body mucosa and also in the serum were evaluated. Eighty patients according to their rapid urease test were divided into two groups: H. pylori positive (n=39) and H. pylori-negative (n=41). The concentrations of cytokines in biopsies and serum were determined by ELISA method. The mean TNF-α and IFN-γ levels in the infected group were significantly higher than that of uninfected patients. In contrast, IL-10 level in most patients was undetectable. The mean antral of stomach TNF-α and IFN-γ levels were significantly higher than that of the stomach body. IFN-γ serum level showed positive correlation with antrum and stomach body levels, whereas no correlation was found in TNF-α in different samples. Higher levels of TNF-α and IFN-γ in antral indicate that the colonization of bacteria in the antrum may be higher than stomach body (culture results from two sites support this statement). Increased serum level of IFN-γ indicates the activation of circulating-T cells against infection. Induced H. pylori-related TNF-α is concentrated is gastric mucosa and this pathogen does not cause any significant change in the serum level of this cytokine. Therefore H. pylori by inducing certain inflammatory cytokines but not IL-10 may contribute the process of disease development.     

Serum IL-6, TNFα and CRP levels in Greek colorectal cancer patients： Prognostic implications

AIM: The significance of preoperative serum IL-6, TNFα and CRP levels in the progression of colorectal cancer (CRC) has not been fully elucidated. Our intention was to investigate their role and identify their prognostic significance.METHODS: The IL-6, TNFα and CRP levels were measured in 74 CRC patients and the relationships between their elevations and both the clinicopathological factors and prognosis of patients were investigated. Serum concentrations of human IL-6 and TNFα were determined by enzyme-linked immunosorbent assay (ELISA). CRP was measured by an immunoturbinometric method. RESULTS: Median IL-6, TNFα and CRP levels were significantly higher in CRC patients than in normal controls. High levels of serum IL-6, TNFα and CRP were correlated with larger tumor size. Furthermore, high IL-6 and high CRP levels were associated with reduced overall survival. CONCLUSION: Serum IL-6, TNFα and CRP levels definitely increase in CRC patients. Pre-operative serum elevation of IL-6 and CRP was thus found to be predictor of the prognosis of CRC patients. The clinical value of TNFα in CRC needs to be further investigated.     

IL-2R、IL-6、IL-8、TNF-α在弥漫性大B细胞淋巴瘤中的变化及其意义

目的:探究细胞因子IL-2R、IL-6、IL-8、TNF-α在弥漫性大B细胞淋巴瘤(DLBCL)中的变化及其意义。方法:收集上海交通大学医学院附属瑞金医院收治的155例R-CHOP(利妥昔单抗、环磷酰胺、阿霉素、长春新碱和泼尼松)方案治疗后达完全缓解且无进展生存期≥24个月的DLBCL患者(持续缓解组)和45例R-CHOP方案治疗后达完全缓解但是在2年内复发的DLBCL患者(复发组)。持续缓解组患者于治疗前、第6个周期治疗开始前以及持续缓解治疗结束1年后采血,复发组患者于治疗前、第6个周期治疗开始前及复发时间点采血。采用IMMUNITE 1000化学发光分析仪检测细胞因子IL-2R、IL-6、IL-8、TNF-α的含量。结果:IL-2R、IL-6及TNF-α含量在Ⅲ～Ⅳ期、ECOG评分≥2分、结外受累个数≥2个、IPI评分≥3分的DLBCL患者中显著升高(P<0.05);IL-8在IPI评分≥3分和双表达淋巴瘤(DEL)的患者中显著升高(P<0.05);IL-6在non-GCB亚型中显著升高(P<0.05)。复发组患者治疗前血清细胞因子IL-2R、IL-6、TNF-α...     

胃癌患者血清IL-6、IL-8和TNF-α水平变化

目的观察胃癌患者血清IL-6、IL-8和TNF-α水平变化。方法采用ELISA法测定80例胃癌（胃癌组）患者、30例胃良性病变（良性组）和20例健康者（正常组）血清IL-6、IL-8和TNF-α水平。结果胃癌患者血清IL-6、IL-8和TNF-α水平明显高于良性组和正常组（P均〈0.05）;Ⅲ、Ⅳ期胃癌患者血清IL-6、IL-8和TNF-α水平明显高于Ⅰ、Ⅱ期（P均〈0.05）;肿瘤根治手术后血清IL-6、IL-8和TNF-α水平较术前明显降低（P均〈0.05）,但仍高于正常对照组（P均〈0.05）。良性组血清IL-6、IL-8和TNF-α水平与正常组差异无显著性（P均〉0.05）。结论胃癌患者IL-6、IL-8和TNF-α水平增高,且增高程度与临床分期有关。     

Serum IL-6, TNFα and CRP levels in Greek colorectal cancer patients: Prognostic implications

AIM: The significance of preoperative serum IL-6, TNFα and CRP levels in the progression of colorectal cancer (CRC) has not been fully elucidated. Our intention was to investigate their role and identify their prognostic significance. METHODS: The IL-6, TNFa and CRP levels were measured in 74 CRC patients and the relationships between their elevations and both the clinicopathological factors and prognosis of patients were investigated. Serum concentrations of human IL-6 and TNFα were determined by enzyme-linked immunosorbent assay (ELISA). CRP was measured by an immunoturbinometric method. RESULTS: Median IL-6, TNFα and CRP levels were significantly higher in CRC patients than in normal controls. High levels of serum IL-6, TNFα and CRP were correlated with larger tumor size. Furthermore, high IL-6 and high CRP levels were associated with reduced overall survival. CONCLUSION: Serum IL-6, TNFα and CRP levels definitely increase in CRC patients. Pre-operative serum elevation of IL-6 and CRP was thus found to be predictor of the prognosis of CRC patients. The clinical value of TNFα in CRC needs to be further investigated.     

胃癌患者血清IL-6、IL-8、TNF-α水平变化及意义

目的观察胃癌患者血清IL-6、IL-8和肿瘤坏死因子α（TNF-α）水平变化,并探讨其临床意义。方法采用ELISA法测定50例胃癌患者（观察组）和20例健康对照者（对照组）血清IL-6、IL-8和TNF-α水平。结果观察组患者血清IL-6、IL-8和TNF-α水平明显高于对照组（P均〈0.05）。观察组Ⅲ、Ⅳ期胃癌患者血清IL-6、IL-8和TNF-α水平明显高于Ⅰ、Ⅱ期患者（P均〈0.05）。结论 胃癌患者IL-6、IL-8和TNF-α水平增高。这可能与胃癌的发生、发展有关。     

Sequential monitoring of serum IL-6, TNF-α, and IFN-γ levels in a CAEBV patient treated by plasma exchange and immunochemotherapy

We report the case of a female patient with chronic active Epstein?CBarr virus infection (CAEBV) accompanied by hemophagocytic syndrome (HPS). On admission, she presented with severe liver dysfunction and disseminated intravascular coagulation with elevation of serum IL-6, TNF-??, and IFN-?? levels. Plasma exchange (PE) followed by immunochemotherapy with prednisolone, cyclosporine A, and VP16 was performed. PE decreased serum cytokine levels dramatically and improved liver function. Following immunochemotherapy, CAEBV became inactive. Four months after discharge, however, CAEBV relapsed with HPS, and serum cytokine levels were extremely elevated again. There was no response to immunochemotherapy, and the patient died 1?day after admission. We examined the cytokines in five additional untreated-CAEBV patients and determined that they were elevated above the normal level in all patients. These results suggest that inflammatory cytokines may have roles in the development of CAEBV, and that their depletion can be an effective treatment for this disease.     

血清IL-6、IL-8、TNF-α表达与急性脑梗死损伤的相关性

目的 探讨急性脑梗死患者血清白细胞介素-6(IL-6)、IL-8、肿瘤坏死因子(TNF-α)水平的动态变化在急性脑梗死发病机制中的作用以及临床意义.方法 应用双抗体夹心酶联免疫吸附法检测49例急性脑梗死患者发病1、4、7、14 d的血清IL-6、IL-8、TNF-α水平,并与对照组进行比较分析.结果 观察组患者发病第1天血清IL-6、IL-8水平即明显高于对照组(P＜0.01),第4天达峰值[(19.53±5.25)、(3.20±1.05) μg/L],之后逐渐下降,第14天水平仍明显高于对照组(P＜0.05).血清TNF-α水平在第1天即达峰值[(59.38±14.37)μg/L],在1-14d始终明显高于对照组(P＜0.05).发病后第1天及第4天血清IL-6、IL-8、TNF-α水平均与梗死体积呈正相关(r=0.373,P＜0.05;r=0.541,P＜0.01;r=0.646,P ＜0.01;r =0.305,P＜0.05).结论 IL-6、IL-8、TNF-α水平的增高程度与神经功能缺损程度及梗死体积大小密切相关,临床检测有助于急性脑梗死的临床诊断及脑损害程度的评估.     

Clinical significance of dynamic detection for serum levels of MCP-1,TNF-α and IL-8 in patients with acute pancreatitis

Objective:To observe dynamic changes of levels of monocyte chemotactic protein-1(MCP-1),tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in patients with acute pancreatitis and to investigate its evaluation value on the severity of acute pancreatitis.Methods:A total of 109 patients with acute pancreatitis admitted were divided into mild acute pancreatitis group(MAP group,42 cases),moderately severe acute pancreatitis(MSAP group,35 cases)and severe acute pancreatitis(SAP group,32 cases).ELISA was used to detect the serum levels of MCP-1,TNF-α and IL-8 of patients at day 1,day 4 and day 7 of admission to hospital.Results:The serum levels of MCP-1,TNF-α and IL-8 from MAP group,MSAP group and SAP group at day 1 of admission to hospital all significantly increased.There was a significant difference between MAP group and control group,MSAP group and MAP group,SAP group and MSAP group(P0.05).The serum concentrations of IL-8 from MASP group and SAP group obviously increased at day 1,and there was significant difference between MASP group and MAP group,SAP group and MSAP group(P0.05),while the difference between MAP group and control group was not obvious(P0.05);The serum concentrations of MCP-1,TNF-α and IL-8 from MAP group all reached the highest level at day 4,which were significantly higher than the detection levels at day 1.In MSAP group and SAP group,the serum concentrations of MCP-1,TNF-α and IL-8 were the highest at day 1,which were significantly higher than the detection levels at day 4 and 7.At each detecting timing,the serum concentrations of MCP-1,TNF-α and IL-8 from MSAP group and SAP group were all higher than those of MAP group and MSAP group,respectively.Conclusions:The dynamic changes of serum levels of MCP-1,TNF-α and IL-8 in patients with acute pancreatitis have their rules,and the change rule of MAP group was different with that of MSAP and SAP group,which showed the reference value for the diagnosis and illness severity evaluation of acute pancreatitis.     

HSP患儿血清LTB4、IL-6、IL-8与TNF-α水平测定及分析

目的探讨血清白三烯B4（LTB4）、IL-6、IL-8及TNF-α水平变化在过敏性紫癜（HSP）发病中的作用。方法选择42例HSP患儿和20例健康儿童（对照组）,采用双抗体夹心ELISA法测定血清LTB4、IL-6、IL-8与TNF-α水平。结果HSP组血清LTB4、IL-6、IL-8与TNF-α水平均显著高于对照组,且急性期显著高于恢复早期（P均〈0．05）;HSP组并肾损害者血清TNF-α水平显著高于无肾损害者（P〈0．05）。结论LTB4、IL-6、IL-8、TNF-α水平升高及其相互作用所致免疫病理损伤可能是HSP发病的重要因素,且TNF-α可能参与了继发性。肾脏损害。