口腔白色念珠菌病的临床分析

目的 进一步探讨口腔念珠菌病的易感因素,加深对念珠菌病的认识,为临床正确合理治疗奠定基础。方法 对134例白色念珠菌感染的病例进行口腔粘膜病变区及义齿承找区唾液细菌培养。结果 白色念珠菌感染的病人常与全身和局部因素的密切关系。结论 白色念珠菌感染的病人多见于老年人,尤其是戴义齿修复者占多数,其次为年老体弱、婴幼儿、长期应用广谱抗生素以及颈部放疗、肿瘤化疗者。     

口腔念珠菌病的治疗体会

我们在临床上分别使用氟康唑和制霉菌素治疗口腔白色念珠菌病 ,并注意改善口腔局部环境 ,调理全身状况 ,均取得较好的疗效 ,现报道如下 :1　材料与方法1.1　一般资料选取我院黏膜科门诊收治的口腔白色念珠菌病患者 90例 ,均经涂片镜检查见念珠菌菌丝或Ｓａｂｏｕｒａｕｄ培养基培养菌落多于 2 0 0个 ,并有临床症状者。其中急性假膜型 15例 ,急性红斑型 2 7例 ,慢性红斑型 48例。年龄 2～ 78岁 ,平均 49.4岁 ,男 5 4例 ,女 3 6例。1.2　分组按随机数字表分为 2组 ,Ａ组 45例使用制霉菌素治疗 ,Ｂ组 45例使用氟康唑治疗。1.3　临床资料Ａ组　…     

铁剂在治疗口腔白色念珠菌病中作用的研究

目的 探讨缺铁性贫血与口腔白色念珠菌病间的关系，以及补铁在治疗口腔白色念珠菌病中的作用。方法对145例确诊为口腔白色念珠菌病的患者行血液学检查，将其中46例患有缺铁性贫血者随机分为两组，对照组采用常规抗真菌治疗，实验组采用抗真菌联合补铁治疗。结果145例口腔白色念珠菌病患者中有46例合并缺铁性贫血（31．7％）。实验组近期疗效与对照组无显著性差异（P〉0．05），远期疗效明显优于对照组（P〈0．05）。结论缺铁性贫血是口腔白色念珠菌病的一个重要诱因，对于合并缺铁性贫血的口腔白色念珠菌病患者，联合补铁治疗能够显著提高远期治愈率。     

口腔黏膜病临床治疗Ⅴ.口腔念珠菌病诊断与治疗进展

口腔念珠菌病（oral eandidiasis）是念珠菌属真菌引起的口腔黏膜感染性疾病。近年来，由于抗生素和免疫抑制剂在临床上的广泛使用，发生菌群失调或免疫力降低，导致内脏、皮肤、黏膜被真菌感染的病例日益增多，口腔念珠菌病的发病率也相应增高。     

艾滋病伴发口腔白色念珠菌感染1例

临床病例资料 患者,女,38岁。因舌部白色斑块7个月来我院口腔内科就诊。6^＋月前,患者曾就诊于当地医院,给予抗炎治疗（具体不详）半年,未见好转,故来我院就诊。既往体健,1年前离异,否认有输血史、吸毒史和冶游史,无肝炎、结核病史。     

口腔念珠菌病8例临床病理分析

目的:探讨口腔念珠菌病的临床诊断与病理诊断,以减少误诊.方法:对8例病理诊断为口腔念珠菌病的患者的临床资料进行回顾性分析,并对其标本进行组织病理学HE和PAS染色研究.结果:8例患者病理诊断为口腔念珠菌病,而临床上均未考虑为本病.常规组织病理切片均见上皮内微小脓肿,PAS染色可见真菌菌丝侵入上皮表层.结论:临床上遇到长期不愈的增生或溃疡型病变时,应考虑是否有念珠菌感染;组织病理学检查发现病变上皮内出现微小脓肿时,应高度怀疑本病,PAS染色有助于确诊.     

艾滋病合并口腔念珠菌病31例临床分析

目的：探讨艾滋病合并口腔念珠菌病的临床特点和诊治转归。方法：回顾性研究从2012-09～2013-03北京地坛医院收治的31例艾滋病合并口腔念珠菌感染者的临床资料,包括一般资料、临床特征、口腔表现、CD4细胞计数、机会性感染状况、抗真菌治疗转归等。结果：31例患者中30例 CD4细胞计数小于200个/μl,有29例合并多种机会性感染。临床表现30例为假膜型,1例为红斑型,2例假膜型合并口角炎型。抗真菌治疗后,8例病损缩小,23例病损完全消失,3例停药后复发。结论：艾滋病合并口腔念珠菌病临床上多见于 CD4细胞＜200个/μl 的患者,临床表现以假膜型为主,常合并有其他部位的多种机会性感染。这类患者抗真菌治疗有效。     

老年人口腔念珠菌病的临床观察

口腔念珠菌病是较为常见的病,其致病菌主要是白色念珠菌,正常人口腔带菌情况,各文献报告颇不一致,国内资料约6.4～8.83%,国外报告高达50%,但不致病,必须在某些全身、局部诱因条件下可转化为致病菌而导致感染,老年体弱病人具有发病诱因条件较多。本文总结1986年以来确诊为念珠菌感染的病例47例,分析其诱因、     

白色念珠菌与口腔上皮癌变的关系

白色念珠菌是口腔内常驻菌和机会致病菌,它作为口腔粘膜多种感染的致病因子的作用已得到肯定,但它在口腔上皮癌发生发展中的作用尚存在争议,有部分学者认为它起到了促进的作用,而又有学者认为它仅仅是继发于癌前损害或肿瘤的感染。本文就白色念珠菌与口腔上皮癌发生发...     

口腔白色念珠菌感染相关因素分析

本资料总结了1995年以来经临床检查和细菌培养确诊为口腔白色念珠菌感染的病例334例,其中男性238例,女性96例,年龄10～74岁,平均65岁。分析其发病相关因素,结果显示:334例病人中,以口腔局部义齿修复、年老体弱、婴幼儿、全身应用抗生素、头面部放化疗为多,其次是口腔卫生不良、大手术后、严重血液病等(表1)。口腔白色念珠菌感染相关因素分析@胡艳$西安陆军学院干休所!陕西西安710065 @李丽$西安陆军学院干休所!陕西西安710065     

白色念珠菌在义齿软衬材料表面上发育的研究

目的 探讨白色念珠菌在义齿软衬材料上的发育变化。方法 采用日本临床常用的７种软衬材料。通过测定培养基ｐＨ值变化讨论了白色念珠菌的发育情况。结果 虽然有一些材料固有成分对白色念珠菌有一定的抑菌作用,但会随长时间的使用而显著劣化。结论 攻期使用软衬材料必须做正确的义齿菌斑清除。     

新生儿口腔中白色念珠菌来源的分子遗传学初探

目的 :比较新生儿口腔中与其母亲口腔和阴道白色念珠菌基因型的差异 ,探讨新生儿口腔中白色念珠菌的来源。方法 :采集 2 0 8例产妇产前 48h以内的口腔含漱液标本和阴道拭子标本及其足月新生儿 1、3、5d和 1月的口腔拭子标本 ,进行白色念珠菌的培养和鉴定。再选取母子均分离出白色念珠菌的标本进行DNA提取和随机引物扩增DNA多态性分析法 (RAPD)分析 ,比较基因型是否具有差异。结果 :产后 5d内仅有不到 1%的新生儿口腔中检出了念珠菌 ,产后 1月新生儿口腔中白色念珠菌的检出率增高为 2 1.3 9%。并且 ,从新生儿分离得到的白色念珠菌的基因型与从其母亲口腔或阴道分离得到的白色念珠菌的基因型均不相同。结论 :母婴垂直传播可能并非新生儿口腔中的白色念珠菌的主要定植来源     

The in vitro Iysozyme susceptibility of Candida albicans cultured in carbohydratesupplemented media

The in vitro lysozyme susceptibility of three oral isolates of Candida albicans cultured in carbohydrate-supplemented media was studied. Lysozyme was shown to have a dose- and time-dependent killing effect on C. albicans isolates. Fungieidal activity persisted to varying degrees when yeast isolates were cultured in a variety of carbohydrates (glucose, galascrose. sucrose, maltose. xylitol and laelose) before exposure to 20 μg/ml lysozyme. Sucrose and galactose grown yeasts exhibited increased resistance to iysozyne conipared with (in decreasing order) those grown in glucose, maltose, xylnol or laelose. Further, the C albicans isolates tested demonstrated strain variations in their susceptibility to lysozyme. These results suggesl that dietary carbohydrate may play a role in modulating the yeast cell populations in the oral —– by altering the fungal susceptibility to salivary lysozyme.     

Coaggregation of Candida albicans oral Actinomyces species

Eight strains of Actinomyces were examined for their ability to coaggregate in vitro with four strains of Candida albicans. The Actinomyces coaggregated to various degrees with all of the Candida strains. Exposure of the Candida but not the Actinomyces to heat, trypsin, proteinase K, amphotericin B or trichodermin abolished Coaggregation. All sugars tested did not inhibit any of the reactions. All coaggregating pairs were disaggregated by the addition of SDS, but nonionic detergents had no effect. The addition of urea or EDTA completely reversed Coaggregation. Actinomyces strains were sensitive to periodate oxidation, whereas the Candida strains were unaffected. These data suggest that the coaggregations involve a protein on the Candida surface that may interact with carbohydrates or carbohydrate-containing molecules on the surface of the Actinomyces. These observations expand the known range of intergeneric coaggregations occurring between human oral microbes and indicate that Coaggregation of C. albicans and Actinomyces may be an important factor in oral colonization by this yeast.     

人唾液富组蛋白抗白色念珠菌芽生孢子作用的实验研究

本研究收集健康成年人（２０－３０岁）刺激性腮腺唾液，用生物素凝胶Ｐ－２层析分离出一族纯化的唾液富组蛋白。光密度监测和细胞活力分析证明富组蛋白能抑制或杀灭白色念珠菌芽生孢子。透射电子显微镜观察提示富组蛋白能破坏白色念珠菌细胞膜，诱导胞内物质丧失。从而导致该生物体死亡。     

The effect of two artificial salivas on the adhesion of Candida albicans to heat-polymerized acrylic resin

PURPOSE

Xerostomia can diminish the quality of life, leads to changes in normal chemical composition of saliva and oral microbiata, and increases the risk for opportunistic infections, such as Candida albicans. Various artificial salivas have been considered for patients with xerostomia. However, the knowledge on the antifungal and antiadhesive activity of artificial saliva substitutes is limited. The aim of the present study was to evaluate influence of two artificial salivas on the adhesion of Candida albicans to the polymethylmethacrylate disc specimens.

MATERIALS AND METHODS

Two commercial artificial salivas (Saliva Orthana and Biotene Oral Balance Gel) were selected. 45 polymethylmethacrylate disc specimens were prepared and randomly allocated into 3 groups; Saliva Orthana, Biotene-Oral Balance gel and distilled water. Specimens were stored in the artificial saliva or in the sterile distilled water for 60 minutes at 37℃. Then they were exposed to yeast suspensions including Candida albicans. Yeast cells were counted using ×40 magnification under a light microscope and data were analysed.

RESULTS

Analysis of data indicated statistically significant difference in adhesion of Candida albicans among all experimental groups (P=.000). Findings indicated that Saliva Orthana had higher adhesion scores than the Biotene Oral Balance gel and distilled water (P<.05).

CONCLUSION

In comparison of Saliva Orthana, the use of Biotene Oral Balance Gel including lysozyme, lactoferrin and peroxidase may be an appropriate treatment method to prevent of adhesion of Candida albicans and related infections in patients with xerostomia.     

Clinical and microbiological efficacy of chlorine dioxide in the management of chronic atrophic candidiasis: an open study

OBJECTIVE: To assess the clinical and microbiological efficacy of chlorine dioxide (ClO2) as a topical antiseptic for the treatment of chronic atrophic candidiasis in geriatric patients. PARTICIPANTS: Thirty patients with chronic atrophic candidiasis. METHODS: Patients were instructed to rinse the mouth with 0.8% ClO2 mouth rinse (DioxiDent) twice daily for one minute and to soak their dentures overnight in the ClO2 for 10 days. Patients were evaluated both clinically and microbiologically at baseline and after 10 days, and any significant side effects were recorded. The clinical appearance of the oral soft tissues was scored on a scale of 0-3 (0 indicating no clinical signs, 1 indicating involvement of < 25% of the palatal mucosa, 2 indicating involvement of 25-50% of the palatal mucosa, and 3 indicating marked erythema involving > 50% of the palatal mucosa). Microbiological testing was undertaken to determine the number of colony forming units (CFUs) of Candida albicans. RESULTS: ClO2 significantly improved the clinical appearance and microbial count (p < 0.001) after treatment, without significant side effects. Results showed marked improvement in the clinical appearance of the tissues after 10 days, with total resolution in the majority of cases. The total CFU/ml ranged from 15,000-53,000 at baseline and was reduced to < or = 500 after 10 days of treatment (p < 0.001). The mean clinical score was 2.50 at baseline, and was reduced to 0.17 after 10 days of treatment (p < 0.001). CONCLUSIONS: Within the limitations of this pilot study, the effectiveness of topical chlorine dioxide (0.8%) in the management of chronic atrophic candidiasis was demonstrated. ClO2 provided a safe and clinically effective option in the management of chronic atrophic candidiasis.