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目的 分析非小细胞肺癌(NSCLC)癌组织和癌旁组织的环状核糖核酸(circRNA)表达水平与诊断价值。方法选取2020年6月至2021年5月江西省胸科医院收治的12例NSCLC患者的病理组织标本,筛选出癌组织和癌旁组织中表达差距明显的circRNA,制作火山图,同时予以聚类分析。收集同期47例NSCLC患者与47例健康体检者血清,均予以circRNA检测,制作受试者工作特征(ROC)曲线,探究NSCLC的circRNA诊断价值。标注曲线下面积(AUC),探究血清circRNA表达水平与临床指标相关性。结果 筛选出癌组织和癌旁组织中表达差距明显的circRNA共194个,当中121个显示上调,73个显示下调,通过火山图及聚类分析结果显示,circRNA能够清晰分辨NSCLC癌组织和癌旁组织。不同性别、年龄、吸烟情况、肿瘤分化、病理类型、癌症分期NSCLC患者的circRNA表达水平比较,差异无统计学意义(P>0.05)。ROC曲线研究结果 显示,血清circRNA诊断NSCLC的灵敏度为82.98%,特异度为61.70%,AUC为0.795。结论 circRNA在NSCLC癌组织... 相似文献
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结肠癌组织MRP表达及意义 总被引:1,自引:0,他引:1
目的 研究MRP在结肠癌组织、癌旁组织、正常组织中的表达情况,并分析其意义。方法 应用S—P免疫组化方法,检测MRP在上述三种组织中的表达,并进行统计分析。结果 结肠癌组织MRP有显著高表达,其表达率随结肠癌分化程度呈负相关而与结肠癌分期及性别等无关;癌旁组织中MRP表达率亦显著高于正常组织。结论 结肠癌存在MRP介导的化疗耐药机制,并提示其与结肠癌细胞转变分化有关,临床上可用于预测患者化疗敏感性及迫切性,从而使化疗个体化,并估计预后。 相似文献
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目的:探讨直肠癌,直肠腺瘤和癌旁组织中P^21的表达及意义。方法:应用ABC免疫组化方法检测21例直肠腺癌和癌旁组织,14例直肠腺瘤的P^21表达。结果:P^21在直肠腺癌,直肠腺瘤和癌旁组织中的阳性表达分别为76.2%、50%和19%。腺癌与癌旁组织的表达有明显差异(P〈0.05),腺癌的增强表达与分化程度,Duke‘s分期,淋巴结转移无关,腺瘤组织中不典型增生区域有表达。结论:P^21表达与直 相似文献
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目的探讨肝细胞癌(HCC)癌旁组织中nm23-H1表达强度与HCC门静脉转移的关系。方法选择HCC111例,其中门静脉转移、无肝内外转移者各33例,肝外转移45例,另选肝血管瘤(HH)患者30例设为对照,用免疫组化方法检测上述患者癌旁或瘤旁组织的nm23-H1蛋白的表达,采用等级相关分析探讨nm23-H1蛋白表达与门静脉转移之间的关系。结果HCC伴门静脉转移组nm23-H1蛋白表达程度明显低于HCC无肝内外转移组及伴肝外转移组(P<0.05),HCC无肝内外转移组及伴肝外转移组nm23-H1蛋白表达程度明显低于对照组(P<0.05),nm23-H1蛋白表达程度在HCC无肝内外转移组与伴肝外转移组之间无显著性差异(P>0.05)。结论HCC癌旁组织nm23-H1表达程度与门静脉转移有关,nm23-H1表达程度低发生门静脉转移的可能性大。 相似文献
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Fan ZHANG Zhuo-min WANG Hong-yu LIU Yun BAI Sen WEI Ying LI Min WANG Jun CHEN Qing-hua ZHOU 《Acta pharmacologica Sinica》2010,31(1):111-117
Aim:
To analyze gene expression in formalin-fixed, paraffin-embedded lung cancer tissues using modified method.Methods:
Total RNA from frozen tissues was extracted using TRIZOL reagent. RNA was extracted from formalin-fixed, paraffin-embedded tissues by digestion with proteinase K before the acid-phenol:chloroform extraction and carrier precipitation. We modified this method by using a higher concentration of proteinase K and a longer digestion time, optimized to 16 hours. RT-PCR and real-time RT-PCR were used to check reproducibility and the concordance between frozen and paraffin-embedded samples.Results:
The results showed that the RNA extracted from the paraffin-embedded lung tissues had high quality with the most fragment length between 28S and 18S bands (about 1000 to 2000 bases). The housekeeping gene GUSB exhibited low variation of expression in frozen and paraffin-embedded lung tissues, whereas PGK1 had the lowest variation in lymphoma tissues. Furthermore, real-time PCR analysis of the expression of known prognostic genes in non-small cell lung carcinoma (NSCLC) demonstrated an extremely high correlation (r>0.880) between the paired frozen and formalin-fixed, paraffin-embedded specimens.Conclusion:
This improved method of RNA extraction is suitable for real-time quantitative RT-PCR, and may be used for global gene expression profiling of paraffin-embedded tissues. 相似文献12.
目的探讨survivin、p53在卵巢上皮性癌组织中的表达及其临床意义。方法应用SP免疫组化法测定46例卵巢上皮性癌组织中survivin、p53的表达强度,并与临床指标进行对照分析,同期44例卵巢上皮性良性肿瘤为对照组。结果①survivin在卵巢上皮性良性肿瘤组织中的表达率为11.4%(5/44),在卵巢上皮性癌组织中的表达率为88.6%(39/46),两组比较有统计学差异(P<0.01);survivin表达强度与癌细胞分化程度以及是否有淋巴结转移存在相关性,细胞分化程度低、有淋巴结转移者表达强度高于细胞分化程度高、无淋巴结转移者;②p53在卵巢上皮性良性肿瘤组织中的阳性表达率为11.4%(5/44),在卵巢上皮性癌组织中的表达率为78.3%(36/46),两组比较有统计学差异(P<0.01);p53表达强度与卵巢癌的临床分期以及是否有淋巴结转移存在相关性,临床分期晚、有淋巴结转移者表达强度高于早期、无淋巴结转移者;③survivin、p53在卵巢上皮性癌组织中的表达存在正相关。结论 survivin、p53在卵巢上皮性癌组织中呈高表达,二者表达存在正相关,并且提示恶性程度高,预后不良。 相似文献
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survivin基因在化疗药物诱导的肺癌细胞凋亡中的作用 总被引:7,自引:0,他引:7
目的 探讨survivin基因在化疗药物顺铂 (DDP)和足叶乙苷 (VP16 )诱导的肺癌细胞凋亡中的作用。方法 用肺腺癌A5 4 9细胞株进行培养传代 ,MTT试验检测DDP和VP16不同药物浓度对肺癌细胞生长的抑制作用 ,实验分为 :DDP、VP16和对照组。DDP和VP16组的肺癌细胞又分为高浓度和低浓度化疗药物处理组。用逆转率PCR检测sur vivin基因的表达。同时利用流式细胞术定量检测细胞凋亡。结果 不同浓度VP16和DDP对肺癌细胞生长均有明显的抑制作用 ,并有浓度和时间的依赖性。化疗药物组细胞凋亡率和survivin基因表达抑制率高于对照组 ,也有时间依赖性。结论 survivin基因的抑制在DDP和VP16诱导肺癌细胞凋亡中起重要作用 相似文献
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《Expert opinion on therapeutic patents》2013,23(12):1723-1737
Importance of the field: Survivin is a prominent anti-apoptotic molecule expressed widely in the majority of cancers. Overexpression of survivin leads to uncontrolled cancer cell growth and drug resistance. Efficient downregulation of survivin expression and its functions can sensitise the tumour cells to various therapeutic interventions such as chemotherapeutic agents leading to cell apoptosis. Areas covered in this review: The article thoroughly analyses up-to-date information on the knowledge generated from the survivin patents. Various key areas of research in terms of understanding survivin biology and its targeting are discussed in detail. What the reader will gain: The article clearly gives an insight on the recent developments undertaken to understand the roles of survivin in cancer and in validating various treatment paradigms that suppress survivin expression in cancer cells. Take home message: Most recent developments are helpful for effectively downregulating survivin expression by using various therapeutic platforms such as chemotherapeutic drugs, immunotechnology, antisense, dominant negative survivin mutant, RNA interference and peptide-based methods. However, selective and specific targeting of survivin in cancer cells still poses a major challenge. Nanotechnology-based platforms are currently under development to enable site-specific targeting of survivin in tumour cells. 相似文献
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原发性非小细胞肺癌中PCNA及survivin的表达及临床意义 总被引:2,自引:1,他引:1
目的探讨非小细胞肺癌(Non—small cell lung cancer,NSCLC)中增殖细胞核抗体(Proliferating Cell Nuclear Antigen,PCNA)及凋亡抑制基因survivin的表达对患者预后的影响。方法采用免疫组织化学SP法,检测92例非小细胞肺癌组织中PCNA基因及survivin基因的表达,分析PCNA及survivin的表达与非小细胞肺癌临床病理特征之间的关系及其与患者预后的关系。结果92例非小细胞肺癌中PCNA阳性表达率为60.9(56/92),其表达与肿瘤大小、组织学类型、有无淋巴。结转移、临床病理分期等无关,而与NSCLC的组织学分级及原发肿瘤T分期有关,在组织学分级为Ⅲ级的肿瘤中的表达明显高于Ⅰ+Ⅱ级的肿瘤中的表达,差异有统计学意义(66.7%vs52.6%,x^2=4.269,P=0.039);在T3+T4期中的阳性表达率明显高于在T1+T2期中的表达率,差异有统计学意义(75.8%vs52.5%,x^2=4.789,P=0.029)。survivin阳性表达率为46.7%(43/92),其表达与肿瘤大小、有无淋巴结转移、临床病理分期以及组织学类型等无关,而与NSCLC的组织学分级有关,在组织学分级为Ⅲ级的肿瘤中的表达明显高于Ⅰ+Ⅱ级的肿瘤组织中的表达,差异有统计学意义(52.1%vs37.5%,x^2=3.975,P=0.046)。单因素生存分析显示PCNA阳性组及survivin阳性组的总生存时间均低于PCNA阴性组及survivin阴性组,差异有统计学意义(P=0.025及0.033)。结论PCNA和survivin表达是NSCLC患者预后不良因素之一,联合检测PCNA和survivin并结合临床病理分期、组织学分级可以更准确地评价非小细胞肺癌患者的预后。 相似文献
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存活素、血管内皮生长因子-C在结肠癌中的表达及其临床意义 总被引:2,自引:0,他引:2
目的探讨结肠癌组织中存活素(survivin)、血管内皮生长因子-C(VEGF-C)的表达,及其相互关系和临床意义。方法应用免疫组化法检测35例结肠癌组织和15例癌旁组织中survivin及VEGF-C的表达水平。结果结肠癌组织中survivin、VEGF-C阳性表达率分别为60.0%、65.7%显著高于癌旁非癌组织的13.3%(P〈0.05)。survivin、VEGF-C蛋白的表达与结肠癌的浸润深度、淋巴结转移及TNM分期有关(P〈0.05或P〈0.01),与患者性别、年龄、结肠癌分化程度、远处转移无关(P〉0.05)。survivin、VEGF-C的表达呈正相关(P〈0.01)。结论结肠癌组织中survivin、VEGF-C表达均增高并且呈正相关。两者在结肠癌淋巴结转移中有协同效应,共同促进结肠癌的淋巴转移。 相似文献
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目的探讨凋亡相关基因survivin在NSCLC中的表达及其与p53的关系。方法选取52例NSCLC患者组织。检测survivin及p53蛋白的表达。结果正常肺组织中survivin的阳性表达率为0.00%,NSCLC中表达率为65.39%;survivin在p53阳性表达组和阴性表达组中的表达率分别为80%和35.29%,二者比较差异有统计学意义。结论 survivin过度表达提示NSCLC预后不良;p53的失活和survivin的过度表达可能在NSCLC癌变中起协同作用。 相似文献
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目的 探讨微小RNA-205(miRNA-205)在非小细胞肺癌(NSCLC)组织和血浆中的表达和临床意义.方法 用实时定量PCR法测定31例NSCLC患者(试验组)及10例肺良性病变患者(对照组)支气管镜活检组织和血浆中miRNA-205的表达量 ,分析其与各临床病理资料之间关系 ,评价miRNA-205在NSCLC的诊断价值.结果 试验组活检组织及血浆miRNA-205表达量均高于对照组(P<0 .05).miRNA-205在鳞癌组织中的表达高于腺癌(P<0 .05).miRNA-205的表达量与NSCLC患者临床病理特征无明显相关( rs=0 .255 ,P=0 .113 ).ROC曲线分析显示 ,取界值0.932时,组织miRNA-205表达诊断NSCLC的灵敏度和特异度分别为81.3% 和83.3% ;取临界值0.610时 ,血浆miRNA-205表达诊断NSCLC的灵敏度和特异度分别为81.3% 和84.2% .结论 miRNA-205在NSCLC组织和血浆均有较高的表达 ,可能会成为诊断NSCLC ,尤其是鳞癌的参考指标. 相似文献
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目的对肺癌组织中5种微量元素检测指标进行综合分析,探讨其在肺癌发生发展中的作用,为肺癌的早期诊断和治疗提供一定的理论基础。方法以Pubmed、Embase、CNKI、维普数据库和万方等数据库全面检索1985-2012年收录的已发表的相关文献,纳入的研究应包括肺癌组织和癌旁组织,微量元素的检测至少包括cd、cr、Pb、As和se中的一个,使用Review anger5.0软件和Statal2.0软件进行分析。结果共纳入11篇文献,与癌旁组织相比,肺癌组织中cd的含量降低了0.25μg·g^-195%CI:-0.47,-0.03;P=0.03),Se的含量升高了0.17μg^-195%CI:0.01,0.32;P=0.03),Cr、As和Pb的含量在两组间无统计学差异(P〉0.05)。结论肺癌患者组织内微量元素含量的差异,对肺癌的早期诊断和治疗具有一定的参考价值。 相似文献
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Survivin was firstly separated in hybridization of Effector Cell Protease Receptor-1(EPR-1)cDNA in human genome by Yale University's Ambrosini in 1997,which is member of the inhibitor of apoptosis proteins(IAPS).Unlike other IAP protein,found during embryonic and fetal development,survivin wascompletely down-regulated and undetectable in normal adult tissues,and became prominentlyre expressed in all of the most common cancers.It through includes the cysteine/histidine rod-shaped viral IAP repetition sequence baculoviral IAP repeats(BIRs)the structure territory directly or intervenes Caspases the function to display indirectly its anti-perishes weakly the function,simultaneously it also is in the cell division process the chromosome traveler protein(chromosome passenger protein).There are three approaches by which survivin inhibits the processing of apoptosis:(1)inhibits processing of down stream effector caspase-3,caspase-7and caspase-9 in cell receiving apoptotic stimuls;(2)with the Smac/DIABLO function,sends the XIAP activeness to increase,XIAP through directly affects and restrains its function with caspases,achieved restrains function which perishes weakly;(3)through restrains p53 the function to block perishes weakly the process.Survivin expressed specificity and its function multiplicity.Survivin only expresses in tumor tissues and cannot be found in normal terminally differentiated tissues.This kind of expression is been extremely low the cell cycle strict regulation in the G1 time expression,the S time is G1 time 6 times,the G2/M time advances to 40 times,demonstrated its expression has the G2/M time dependence specificity.It has bi-function of inhibiting apoptosis and involving in cell cycle control.Survivin has found in most of tumor cells in recently researches.Survivin expresses generally in all tumor cell line in the American State-run Cancer Research institute anti-tumor medicine screening procedure 60 different tumor cells,in which expresses in the mammary gland cancer cell and the lung cancer cell highly,expresses in the kidney cancer lowly.Survivin except in tumor cell high expression,at breast cancer,tumor in front of and so on endometrium cancer,cervical cancer cancers in the pathological change has the expression.Survivin is over expressed in early malignant,which brokes the balance of proliferation and apoptotic,causes the occurrence of tumor.Obviously,survivin is closely related to the uncontrolled growth carcinogenesis,development and tumorigenesis.Because Survivin in tumor organization's special expression and perishes weakly in the suppression cell and adjusts in the cell division the vital role,causes it to become one to have the latent application value tumor designated object and the tumor treatment new target spot.The recent research of survivin in the development,treatment and situation of tumor is introduced. 相似文献