高效液相色谱法同时测定大豆异黄酮多种组分

目的建立同时测定保健食品中大豆异黄酮多种组分的高效液相色谱法。方法样品中的大豆异黄酮采用甲醇＋水提取,经C18柱分离,在紫外检测器254nm波长处检测。结果此方法的回收率为91．9％～102．0％,RSD为2．5％。结论此方法操作简单,准确可靠,为保健食品中大豆异黄酮含量的检测提供参考。     

高效液相色谱法测定保健食品中大豆异黄酮

目的：建立测定保健食品中大豆异黄酮含量的高效液相色谱法。方法：通过对流动相的优选，以甲醇：水=3：2为流动相，使染料木素、大豆苷元、大豆甙、染料木甙4种大豆异黄酮主要成分达到较好的分离效果，可分别测定4种成分含量及以此4种成分为主要成分的保健食品中大豆异黄酮含量。结果：方法的最低检出浓度分别为大豆甙1．9μg／ml、染料木甙1．2μg／ml、大豆苷元1．0μg／ml、染料木素0．8μg／ml；标准曲线线性范围分别为1．9—100μg／ml、1．2—100μg／ml、1．0—170μg／ml、0．8～140μg／ml；方法平均回收率分别为99．1％、98．5％、97．5％、98．8％；相对标准偏差均（10％。结论：建立的方法可准确、快速的测定大豆异黄酮的含量。     

高效液相色谱法测定保健食品中大豆异黄酮含量的方法改进

目的建立以改进的高效液相色谱法测定保健食品中大豆异黄酮含量的方法。方法固体样品经70%乙醇超生提取30 min,过滤膜后进行液相色谱测定,液体样品过滤后直接进行液相色谱测定。色谱柱：Agilent ZORB-AX SB-C18（250 mm×4.6 mm,5μm）;流动相：甲醇和1%磷酸水溶液梯度洗脱;流速：1.0 ml/min;柱温：30℃;二极管阵列检测器,定量波长254 nm。结果大豆苷、大豆黄素、染料木苷、大豆素、大豆黄苷、染料木素6种组分的回收率在97.5%～105.0%之间,RSD在0.46%～3.33%之间,6种组分检出限分别为0.60、0.57、0.26、0.28、0.55、0.27μg/ml。结论该方法简便、快速、重现性好。     

大豆异黄酮的测定方法研究

目的:检测大豆及其制品中黄豆甙、大黄豆甙、染料木甙、黄豆甙元、大黄豆素和金雀异黄素,建立大豆及其制品中6种大豆异黄酮的高效液相色谱-二极管阵列检测器(HPLC-PDA)检测方法。方法:保健食品、豆腐等样品中的大豆异黄酮直接用甲醇水(80+20,v/v)超声提取30 m in,定容过0.45μm滤膜后测定;豆粉和豆奶粉等样品中的大豆异黄酮及其酯类用甲醇水(80+20,v/v)超声提取,提取液经碱性皂化,将某些酯类转化成对应的大豆异黄酮,经盐酸中和,甲醇水(80+20,v/v)定容,过滤膜后测定;酱油和豆豉等样品超声提取30 m in,提取液经OASIS HLB柱去杂质,过0.45μm滤膜,进行液相色谱分析。样品中的6种大豆异黄酮用甲醇和醋酸铵(0.025 mol/L,pH 4.1)作流动相,流速2.0 m l/m in进行梯度洗脱,经250×4.6 mm粒径10μm C18柱分离,二极管阵列检测器(210~400 nm)测定。30 m in内6种大豆异黄酮可得到良好分离。结果:测定6种大豆异黄酮的平均加标回收率在86.7%~116%,加标回收相对标准偏差均小于8%,最底检出限量小于0.04μg。结论:本方法适用于大豆及其制品中大豆异黄酮的测定。     

高效液相色谱法测定葛根类保健食品中葛根素、大豆苷、大豆苷元及染料木素

目的建立同时测定保健食品中葛根素、大豆苷、大豆苷元和染料木素含量的高效液相色谱测定法。方法选用PhenomenexLunaC18色谱柱（250mm×4．60mm,5μm）,甲醇一0．1％三氟乙酸（TFA）水溶液为流动相梯度洗脱,流速1．0ml／min,检测波长250nm。液相色谱仪测定。结果葛根素、大豆苷、大豆苷元和染料木素的质量浓度在0．1μg／ml～100μg／ml时与峰面积有良好线性关系,方法的回收率在90％一107％。结论高效液相色谱测定法简便、准确,重现性好,适用于含葛根素、大豆苷、大豆苷元和染料木素保健食品的质量控制。     

大豆及其制品中12种大豆异黄酮的HPLC及HPLC-MS法测定研究

目的:建立简便、灵敏、准确的测定大豆及其制品中12种大豆异黄酮的高效液相色谱及液-质联用检测方法,并用于大豆及其制品中大豆异黄酮的检测。方法:大豆及其制品经乙腈/水室温下超声辅助提取后,采用HypersilAA-ODS柱(200mm×2.1mm×5μm),以含0.05%三氟醋酸的乙腈/水梯度洗脱,DAD260nm检测,质谱辅助定性,内标法定量。结果:各组分峰面积和内标峰面积的比值与其相应浓度均呈良好的线性关系,r>0.9990。以豆奶粉为基质,各种大豆异黄酮的加标平均回收率均大于90%,RSD在0.35%～14.5%之间。结论:该法操作简便、快速、结果准确、可靠,适用于实际样品的测定。     

HPLC法测定保健食品中虫草素和腺苷含量

目的：应用高效液相色谱法一次进样同时测定保健食品中的腺苷和虫草素。方法：样品用水超声提取，色谱柱为ZORBAX XDB-C18柱，以磷酸盐缓冲液（0、125％磷酸氢二钾与磷酸二氢钾混合）-甲醇（92：8）为流动相，流量1.0ml／min，检测波长为260nm。结果：腺苷和虫草素在4～350ng范围内呈良好线性，相关系数分别为0.999994和0.999992；检出限均为0．5ng；回收率在99．5％-98．8％之间。结论：该方法操作简便、快速、结果准确、可靠，可作为保健食品中腺苷和虫草素的质量控制监测。     

应用高效液相色谱法测定保健食品中大豆异黄酮的含量

大豆异黄酮是一种从大豆中分离提取的植物雌性激素,具有抗氧化、改善心血管功能等功效。目前发现的大豆异黄酮分为游离型的苷元和结合型的糖苷2类,其中以染料木素和大豆苷元为主要活性成分。本实验通过研究用80％乙醇超声提取后,用水定容,采用高效色谱法测定大豆制品类保健食品中大豆异黄酮含量,方法回收率高,易于操作,满足检测要求。     

HPLC法测定食品中的大豆异黄酮

目的为保证保健食品的质量,在比较了现有大豆异黄酮的检测方法的基础上,建立了长期用于日常测定食品中大豆异黄酮的HPLC方法.方法采用Waters Spherisor(R) ODS2色谱柱,柱温50℃,以甲醇-冰醋酸水溶液(4060)为流动相,1.2ml/min的流速,在254nm用紫外检测器进行检测.结果其重复测定结果的相对偏差在2.25%,回收率在85%-110%范围内.结论该法简便、快速、灵敏度高、重现性及稳定性均较好,适用于保健食品中大豆异黄酮的测定.     

高效液相色谱定量分析发酵豆制品中大豆异黄酮和Monacolin K

目的建立高效液相色谱定量分析发酵豆制品中大豆异黄酮和Monacolin K的方法。方法采用polaris C1(82.0mm×100mm,3μm),水(含0.3%H3PO4)、乙腈为流动相,梯度洗脱,流速:0.3ml/min,柱温50℃,测定波长(λ):大豆异黄酮260nm;Monacolin K为237nm。分析了发酵豆制品中大豆异黄酮和Monacolin K。结果在选定色谱条件下大豆异黄酮和Monacolin K其线性范围在0.2～45mg/L,样品加标回收率88.9%～101.1%,相对标准偏差为0.89%～3.19%.结论本方法精密度好,结果可靠.适合于发酵豆制品中大豆异黄酮和Monacolin K的定量分析。     

Trends in meningococcal disease in Italy in 1988

Meningococcal disease in Italy decreased 15% in 1988 from the previous year (290 vs. 342 cases). The decline was particularly evident in military cases (1.7/100,000 in 1988 vs. 5/100,000 in 1987) reflecting the full coverage of bivalent serogroup (A + C) meningococcal polysaccaride vaccine in army recruits, achieved since January 1988. The highest proportion of cases was seen in people older than 25 years of age (25%). Serogroup C constituted 60% of the isolates, while 19% belonged to serogroup B. The proportion of strains resistant to sulphonamides was 45%, while 15% were resistant to Minocycline and none to Rifampin. Out of the five military cases, only one (due to serogroup C) was attributable to the vaccine failure. A single coprimary case, but no secondary cases occurred among civilians. These findings are consistent with the trends reported in Italy in the previous years.     

Benzene in blood and phenol in urine in monitoring benzene exposure in industry

Determinations of benzene concentration in blood and of phenol in urine were made by head-space gas chromatography techniques on samples taken near the end of the work day from two groups of workers potentially exposed to low levels of benzene in the work-place atmosphere. Preliminary results suggest that benzene in blood is more reliable than phenol tests for assessing both exposure and uptake of benzene. Normal values of phenol in urine (10 mg/liter or less) were found in nearly all those cases in which benzene was detected in the blood.     

2015年郑州市饮用水放射性水平状况调查

目的了解郑州市不同地区、不同季节、不同水源类型的放射性水平。方法分别于丰水期和枯水期采集郑州市区黄河水源水、井水源水、丹江口水源水、出厂水和末梢水各一份;以县为单位,每单位采集出厂水、末梢水、水库水、河水、井水各1份,按《生活饮用水生活标准检验方法》(GB/T 5750.13-2006)检测饮用水中总α和总β放射性水平,依据《生活饮用水卫生标准》(GB 5749-2006)进行判定。结果丰水期、枯水期水体总α、总β放射性水平均低于国家标准限值。结论郑州市不同水源类型均处于正常的天然放射性本底水平。     

Occupational stress in nurses in psychiatric institutions in Taiwan

Nurses are known to be exposed to occupational stress. However, occupational stress is not well documented for nurses in psychiatric institutions in Taiwan. A cross-sectional study was conducted to explore the work-related stress and risk factors of nurses in psychiatric institutions in Taiwan. A structured questionnaire was distributed to nurses at five state-owned psychiatric hospitals in Taiwan in 2001. Demographic information, working environment, and personal health status were inquired. Occupational stress was assessed based on the Chinese version of Job Content Questionnaire (JCQ). General health status and mental health were evaluated by the International Quality of Life Assessment Short Form-36 (IQOLA SF-36). A total of 573 questionnaires were disseminated to nurses and 518 (90.4%) were satisfactorily completed by nurses, including 408 female full-time nurses who had been in their current work for more than 6 months. In the past one month, 17.2% of nurses reported being under significant stress often or always. Assault episodes were reported by 45.1% of nurses in the past 6 months. Among the nurses, 16.9%, 25.2%, 50.0%, and 7.8% belong to the "High strain", "Low strain", "Active", and "Passive" groups, respectively. Perceived occupational stress was associated with young age, widowed/divorced/separated marital status, high psychological demand, low workplace support, and threat of assault at work. Lower general health score was associated with low job control, high psychological demand, and perceived occupational stress. A lower mental health score was associated with low job control, high psychological demand, low workplace support, and perceived occupational stress. We concluded that nurses in psychiatric institutions are under significant stress related to work factors.     

Changes in nursing administration in supporting transplantation in Brazil

This historical and bibliographic study aimed to understand how Nursing was organized to support care in transplantation. The HISA, LILACS, BDENF, PERIENF and DEDALUS databases were consulted, and thirteen references were found, ten of which were scientific articles, two were master's dissertations and one was a doctoral thesis. The span of time chosen for study ranges from the date of the first kidney transplant in Brazil (1965), to the date of publication of the last scientific article found in the databases mentioned above (2003). After reading these articles, the ones that were similar in topic were grouped together, thus creating the thematic axis for the presentation of the results. The results showed that the Nursing profession has played an important and active role in transplants ever since the first procedure in 1965.     

2019年中国肿瘤流行病学研究进展

恶性肿瘤已成为中国居民的主要死因之一。近些年来,中国政府积极推进肿瘤预防和控制领域的研究工作,取得了很大的进步。本文简要汇总中国在肿瘤流行病学领域的研究进展,具体包括2019年的肿瘤负担、癌症危险因素及其干预、筛查和早期发现、癌症防治专项行动(2019-2022年)方案等方面,以期为我国肿瘤防治工作的有效开展提供技术支撑和理论依据。     

2012年绵阳市蔬菜中农药残留状况

目的 了解绵阳市2012年市售蔬菜中农药残留情况,为食用蔬菜监管提供依据.方法 全部样品均按照GB/T 5009-2003的方法进行农药的残留检测.依据GB 2763-2005《食品中农药最大残留限量》进行判定.结果 2012年共检测蔬菜165份,农药检出率和超标率分别为72.12％和23.64％.豆类、根茎类、叶菜类、瓜果菜类和食用菌类蔬菜检测农药残留超标率分别为8.00％、23.53％、36.00％、5.26％和27.03％,差异有统计学意义(x2=11.41,P＜0.01).不同种类农药超标率差异有统计学意义(x2 =62.42,P＜0.01).夏季和冬季蔬菜农药超标率差异无统计学意义(x2=3.44,P＞0.05).结论 绵阳市叶菜类蔬菜中农药残留情况较为严重,应采取有效措施,加强蔬菜的监督管理,从源头禁止高毒农药的使用,加大蔬菜种植、销售环节的监测,确保市民食用蔬菜的安全.