应用SDH,LDH检测方法对Ca9—22,MEC—1,Tca8113细胞冷冻损伤…

对体外培养的三种肿瘤细胞进行冷冻处理，并对冷冻后细胞琥珀酸脱氢酶（ＳＤＨ）活力与乳酸脱氢酶（ＬＤＨ）释放量在不同冷冻温度和冻融周期条件下的变化，进行了测定。结果显示：冷冻对三种体外培养的癌细胞有明显的杀伤作用，其有效杀伤的临界温度为－４０℃～－５０℃。两次冻融的细胞杀伤率较高，继续增加冻融次数，细胞死亡率未见明显增加。ＬＤＨ释放测定结果表明，细胞膜的损伤是冷冻后立即发生的，在－４０℃冷冻复温后，９     

MEC—1、Ca9—22、Tca8113细胞的冷冻损伤及胞核变化的研究

本研究对体外培养的三种癌细胞进行冷冻杀伤实验,用MTT法、Feulgen染色、细胞核DNA含量和核面积显微分光光度仪测定等手段,观察细胞冷冻后和损伤变化,及其与冷冻温度和冻融周期的关系。实验的结果显示:冷冻对三种体外培养的癌细胞有明显的杀伤作用,其有效杀伤的临界温度为-40℃～-50℃。两次冻融的细胞杀伤率较高,再增加冻融次数,细胞死亡率未见明显增加。胞核FeuIgen染色和对其用显微分光光度仪测量的结果显示:冷冻能使细胞核内容物DNA产生凝聚、变性、胞核面积缩小,Ca9-22、Tca8113细胞的DNA含量减少。核的这些变化与冷冻溫度有一定相关性,温度越低变化越明显。     

口腔颌面部癌瘤细胞和口腔纤维细胞冷冻敏感性的研究

利用体外培养的６种肿瘤细胞和两种口腔纤维细胞对其进行冷冻处理，并对在不同冷冻条件下冷冻后细胞活力的变化进行了测定。结果显示，冷冻对６种体外培养的癌细胞有明显的杀伤作用，其有效杀伤的临界温度为－４０－－５０℃。正常口腔纤维细胞与癌细胞相比对冷冻有一定的耐受性，这可能是冷冻治疗后创面愈合时瘢痕的组织形成较少原因。     

8—甲氧基补骨脂素对粘液表皮样癌细胞的抑制作用

目的：研究８－甲氧基补骨脂素（８－ＭＯＰ）对粘液表皮样癌细胞（ＭＥＣ－１）的抑制作用。方法：应用常规ＭＴＴ法、细胞计数法和流式细胞术研究８－ＭＯＰ作用于粘液表皮样癌细胞的剂量－效应关系和时间－效应关系以及３０％抑制浓度的药物对细胞群体倍增时间和细胞周期的影响。结果：１）小剂量８－ＭＯＰ在短时间内对ＭＥＣ－１细胞没有抑制作用，当剂量大于２５ｍｇ／Ｌ时，药物的抑制作用呈剂量依赖性；作用时间在２４ｈ～７２ｈ之间时表现出时间依赖性关系；药物的抑制作用有延迟性。２）１２０ｈ作用组３０％抑制浓度ＩＣ３０＝３３．８６ｍｇ／Ｌ，５０％抑制浓度ＩＣ５０＝４５．３ｍｇ／Ｌ，ＲＡＡ值＝２．９。３）它阻止细胞进入Ｓ期完成细胞分裂。结论：ＭＥＣ－１细胞对８－ＭＯＰ较敏感，８－ＭＯＰ在人粘液表皮样癌的防治方面可能有一定意义。     

加温联合化疗对人舌癌SCC—25细胞杀伤作用的实验研究

采用ＭＴＴ法评价了人舌鳞状细胞癌ＳＣＣ－２５细胞对几何常用抗癌药物联合加温的敏感性，正常温度（３７℃）时ＳＣＣ－２５细胞对化疗药物敏感性顺序为：阿霉素，顺铂，丝列霉素Ｃ，博莱霉素，氨甲喋呤，匹莱霉素，５－氟脲嘧啶，４０～４５℃温度范围内，不同抗癌药物联合加温对ＳＣＣ－２５细胞杀伤作用及表现出各自的最佳作用温度，在有效药物浓度条件下，药物浓度增加不能显著地改变的药物联合加温的综合癌细胞杀伤效应。     

加温联合化疗对人舌癌SCC─25细胞杀伤作用的实验研究

采用ＭＴＴ法评价了人舌鳞状细胞癌ＳＣＣ－２５细胞对几种常用抗癌药物联合加温的敏感性，正常温度（３７℃）时ＳＣＣ－２５细胞对化疗药物敏感性顺序为：阿霉素、顺铂、丝列霉素Ｃ、博莱霉素、氨甲喋呤、匹莱霉素、５－氟脲嘧啶；４０～４５℃温度范围内，不同抗癌药物联合加温对ＳＣＣ－２５细胞杀伤作用表现出各自的最佳作用温度，在有效药物浓度条件下，药物浓度增加不能显著地改变药物联合加温的综合癌细胞杀伤效应。     

不同稀释剂及其比例对封闭剂性能的影响

选用３Ｇ（二甲基丙烯酸二缩三乙二醇酯）、ＥＤＭＡ（甲基丙烯酸乙二醇酯）两种稀释剂，并设计４０％、５０％、５５％、６０％、７０％、８０％６种不同重量比例封闭剂配方，测定粘结性能（包括粘度值、渗透性、抗剪粘结强度）、机械性能（包括表面硬度、抗压强度、径向抗张强度）、吸水性和耐磨性。结果表明：３Ｇ作稀释剂优于ＥＤＭＡ，不同稀释剂比例（在４０％～６０％范围内）对封闭剂性能影响不大，总体评价，以４０％～５０％配方比例为最好。     

三氧化二砷诱导舌鳞状细胞癌Tca8113细胞凋亡的实验研究

目的：观察三氧化二砷对舌癌细胞系Tca8113的诱导凋亡作用。方法：以人舌鳞癌细胞Tca8113为研究对象，使用荧光染色光镜、透射电镜观察三氧化二砷不同浓度、不同时间作用后的肿瘤细胞的形态学变化，流式细胞仪检测细胞凋亡率。结果：一定浓度的三氧化二砷作用Tca8113细胞24h、48h后，细胞形态学观察可见有明显的细胞凋亡发生；流工细胞仪检测Tca8113细胞的基础凋亡率为0．5％－1．2％，三氧化二砷作用后肿瘤细胞的凋亡率可提高到15％－20％。结论：三氧化二砷可诱导人舌鳞癌细胞系细胞凋亡。     

低温生物介电谱在冷冻外科的应用研究

作者们在冷冻治疗裸鼠移植瘤过程中测量了生物组织的介电常数。低温生物介电谱揭示：组织的相变过程是连续的，从０℃～－８０℃。第三次冻融周期的相变温度明显高于首次冻融。作者相信，介电常数测量对于监测生物膜系统在冷冻过程中的动态变化是很有用的。     

Cariostat测试法中菌斑孵育时间选择的探讨

目的：了解机体龋病活跃性与其患龋状况的关系；分析Ｃａｒｉｏｓｔａｔ法２４ｈ、４８ｈ两种菌斑孵育时间所获的结果及其间的转换状态，确定合适的孵育时间。方法：对３９１名３岁～５岁幼儿进行龋病检查和Ｃａｒｉｏｓｔａｔ法龋病活跃性测试，观察菌斑孵育２４ｈ、４８ｈ的检测结果。结果：Ｃａｒｉｏｓｔａｔ法中菌斑孵育２４ｈ、４８ｈ的各度和患龋率、ｄｆｔ、ＣＳＩ均有高度相关性（Ｐ＜０．００１），４８ｈ的相关数值更大；孵育４８ｈ者各度患龋状况的差异分析亦比孵育２４ｈ者明显；孵育２４ｈ呈１度者有４６．５０％在４８ｈ时呈２度，４８ｈ呈２度者有６３．８５％在２４ｈ时呈０度或１度。结论：Ｃａｒｉｏｓｔａｔ能反映机体之患龋现状，其４８ｈ的菌斑孵育结果更能确切地体现机体的龋病活跃性     

柠檬精油对牙周致病菌抑制作用及其安全性初步研究

目的:探讨柠檬精油对牙周致病菌的体外抗菌活性及对细胞增殖的影响。方法:采用微量液体稀释法测定柠檬精油对Pg、Fn、Aa、Pi的最小抑菌浓度(minimal inhibitory concentration,MIC)及最小杀菌浓度(minimum bactericidal concentration,MBC);以较低浓度的MIC为标准稀释LEO作为实验组,采用MTT法测定柠檬精油对HUVECs的毒性作用,明确抑菌浓度下LEO的安全性。结果:柠檬精油对牙周主要致病菌均有抑菌作用,Pg、Fn、Aa、Pi的MIC分别是9.0 g/L、4.5 g/L、4.5 g/L、9.0 g/L,Aa、Fn的 MBC是9.0 g/L,Pg、Pi的MBC未测得。1/2MIC、1/20MIC浓度的LEO能够抑制人脐静脉内皮细胞的生长,而低于1/200MIC浓度的LEO则对人脐静脉内皮细胞的生长没有影响,其中1/200MIC浓度的LEO作用明显优于0.02%的CHX。结论:体外环境中,柠檬精油对牙周致病菌Pg、Fn、Aa、Pi具有抗菌活性,低浓度应用对机体相对安全。     

Oral cancer: molecular technologies for risk assessment and diagnosis

Purpose: The effective biomarkers related to diagnosis, metastasis, drug resistance and irradiation sensitivity of oral cancers will help the pathologist and oncologist to determine the molecular taxonomy diagnosis and design the individualization treatment for the patients with oral cancers.     

Computer-assisted planning for maxillofacial prosthodontic rehabilitation

Computerized tomography (CT) planning and the use of CT derived surgical templates for implant placement have shown promise for restoring function within months after surgical reconstruction of acquired post-oncologic defects.     

Stimulated whole saliva components in children with Down syndrome

The authors report on the components of stimulated whole saliva from children with Down syndrome—including pH, flow rate, sialic acid and protein concentrations, and amylase and peroxidase activity. Saliva samples were collected from 35 children aged 6–10 years. Of the participants, 17 had Down syndrome and 18 did not. To stimulate saliva production, the children chewed a piece of parafilm for 10 minutes before the sample was collected. Soon after collecting the saliva sample, the authors measured pH using a portable pH-meter. Sialic acid levels were determined with a thiobarbituric acid assay. Protein content was determined with Folin's phenol reagent. Amylase was assayed and the authors measured the maltose produced by the breakdown of starch and peroxidase using ortho-dianisidine.
No statistically significant difference was observed in levels of sialic acid (free and total) between the two groups. Protein concentration was about 36% higher in the group with Down syndrome. However, the salivary flow rate, pH, and amylase and peroxidase activities were lower among the children with Down syndrome.     

Effect of Pingyangmycin on human venous malformation endothelial cells

Purpose: Venous malformations are common vascular anomalies with a propensity of the head and neck. Intralesional injection of Pingyangmycin (PYM, bleomycin A5 hydrochloride) is a widely used sclerotherapy method for the treatment of venous malformation.     

牙髓再生的研究现状

目前,根管治疗术在临床上已得到了广泛的应用并成功的保存了患牙,但其仍有许多术后并发症。随着组织工程技术在口腔领域的应用,构建一种组织工程化牙髓进行牙髓-牙本质复合体的再生治疗成为可能。近年来国内外已有很多关于牙髓再生的研究,并且已经取得了一定成果。本文将对目前国内外牙髓再生的研究进展现状作一综述。     

Oropharyngeal dysphagia in amyotrophic lateral sclerosis alters quality of life

Dysphagia is one of the most important complications encountered in amyotrophic lateral sclerosis (ALS). Our aim was to determine whether oropharyngeal dysphagia impacted the quality of life (QoL) of patients with ALS. Thirty consecutive patients were recruited (31–82 years, 18 men). Swallowing function was evaluated using a standardised videofluoroscopic barium swallow. All the patients completed a specific questionnaire on quality of life in dysphagia (SWAL‐QoL) immediately after the videofluoroscopy. The results of dysphagia outcome severity scale separated 14 patients with oropharyngeal dysphagia and 16 with normal swallowing function. There was no difference in the average age, weight and body mass index of the two groups (dysphagic patients: 68 ± 11 kg versus non‐dysphagic patients: 69 ± 14 kg). Most of the dysphagic patients had a bulbar affection based on their Norris scores which determine the importance of cranial nerves illness (20 ± 8), significantly lower than those of the non‐dysphagic patients (35 ± 5) (P < 0·0001). There was no difference in the neurological peripheral symptoms evaluated by Amyotrophic Lateral Sclerosis Functional Rating Scale scores (dysphagic patients: 26 ± 7 versus non‐dysphagic patients: 27 ± 8) (ns). The swallowing quality of life questionnaire revealed that the dysphagic patients had significant burden (P < 0·001). They were affected by the necessity to applied a food selection (P < 0·01), by the increase in eating duration (P < 0·05) and described a decrease in eating desire (P < 0·05). They complained of fear regarding the risk of dysphagia (P < 0·05). They also described difficulties with oral communication (P < 0·001). All of those complained about dysphagia which impacted directly mental health (P < 0·05) and social life (P < 0·05). In conclusion, oropharyngeal dysphagia is a common symptom accompanying ALS, which alters the patient's QoL, especially social health.     

拔牙位点保存用复合温敏凝胶的构建及体外表征

目的:制备多壁碳纳米管/羟基磷灰石/壳聚糖复合温敏凝胶,探讨其作为拔牙位点保存材料的理化性能。方法:采用原位沉积方法制备多壁碳纳米管/羟基磷灰石(MWNT/ HA简称HAC)复合无机材料,将其分散至壳聚糖温敏凝胶中,制备壳聚糖/多壁碳纳米管/羟基磷灰石(CS /MWNT/HA简称CS/HAC)复合温敏凝胶,以成胶时间、孔径、孔隙率、机械强度及降解性能为指标优化配方设计,并表征复合温敏凝胶的微观形貌及理化性能。结果:以10 g/L的HAC作为补强成分加入到壳聚糖溶液中,能够在不影响成胶并保证一定孔隙率(84%)的前提下,提高材料的机械性能(抗压强度0.441 MPa),所获复合温敏凝胶具有适宜的临床操作时间(12 min),并具有可控的降解速率。结论:负载HAC的复合温敏凝胶具有良好的成型性,并具有较好的机械性能,在拔牙位点保存领域具有良好的应用前景。     

Interventional neuroradiologic procedures in the head and neck

Interventional radiology and interventional neuroradiology are evolving specialties which can aid the head and neck surgeons in the clinical management of patients with complex vascular lesions.