藏红花素对人宫颈癌Hela细胞顺铂耐药性的影响

目的 探讨藏红花素对人宫颈癌Hela细胞顺铂耐药性的影响.方法 将对数生长期耐顺铂人宫颈癌Hela/DDP细胞随机分为空白对照组、藏红花素组、顺铂组及联合组.藏红花素组细胞以含终质量浓度400 mg·L-1藏红花素的培养基培养,顺铂组细胞以含终质量浓度5 mg·L-1顺铂的培养基培养,联合组细胞以含终质量浓度400 m...     

NF-κB活性变化对HL-60细胞凋亡及WT1、Bcl-2基因表达的影响

目的为探讨NF-κB变化对白血病细胞凋亡的影响及其机制。方法 用PDTC抑制HL-60细胞NF-κB的活性，观察NF-κB活性抑制前后阿糖胞苷诱导的HL-60细胞凋亡的变化及WT1、Bcl-2表达水平的变化。结果 NF-κB活性抑制能明显增强阿糖胞苷诱导的HL-60细胞凋亡作用，同时明显下调WT1、Bcl-2的表达。结论 NF-κB活性抑制使白血病细胞凋亡增加，其作用可通过直接下调WT1的表达和间接下调Bcl-2的表达来实现.     

脂质体介导hTERT反义寡核苷酸对HL60细胞增殖及凋亡的影响

目的 研究经脂质体介导转染端粒酶逆转录酶(hTERT)反义寡核苷酸(ASODN)对HL-60细胞增殖、凋亡的影响.方法 运用四唑盐比色(MTT)法检测HL-60细胞的增殖、Annexin VFITC/PI双标记法细胞早期凋亡流式检测法检测HL-60细胞的凋亡.结果 hTERT ASODN组(终浓度10 μmol/L)、脂质体介导hTERT ASODN组(终浓度0.5 μmol/L)均能抑制HL60细胞增殖,诱导HL-60细胞凋亡,两组比较差异无显著性.各ASODN组对HL-60的抑制作用及诱导凋亡作用均弱于阿糖胞苷(终浓度10 μmol/L)(P<0.01).结论 脂质体的介导增强了hTERT AS0DN抑制HL-60细胞增殖及诱导凋亡的作用效果.脂质体介导hTERT ASODN有可能成为化疗外治疗急性白血病的有益补充.     

吡柔比星对HL-60细胞NF-κB p65活性及细胞凋亡的影响

目的研究吡柔比星(perarubicin,THP)对髓系白血病细胞系HL-60细胞凋亡与核因子-κB p65(nucle-ar factor kappa B p65,NF-κB p65)活性的影响。方法指数生长期HL-60细胞置于96孔培养板内RPMI 1640培养液培养2 h后,分为两部分,第1部分又分为A、B 2组。A组加PBS液继续培养12 h,B组分别加1、10、100μmol/L(终浓度)的THP继续培养12 h;用DNA梯状凝胶电泳法(DNA ladder electrophoresis)和流式细胞检测技术(flow cy-tometry,FCM)分别检测HL-60细胞的凋亡率。第2部分也分为A、B 2组,A组加PBS液继续培养3 h,B组分别加1、101、00μmol/L(终浓度)的THP继续培养3 h;用FCM检测NF-κB p65活化率。结果1、10、100μmol/L的THP诱导HL-60细胞的凋亡率分别为(31.78±4.31)%(、47.25±5.27)%(、56.49±1.59)%,组间及与对照组(6.46±1.45)%比较差异有统计学意义(P<0.05);诱导的HL-60细胞NF-κB p65活化率分别为(16.21±1.20)%、(23.98±3.21)%(、32.44±2.89)%,组间及与对照组(8.44±2.20)%比较差异有统计学意义(P<0.05)。DNA梯状凝胶电泳Ladder出现从多到少的顺序为:100μmol/L THP1、0μmol/L THP、1μmol/L THP、对照组。结论THP在促进HL-60细胞凋亡的同时有诱导其NF-κB p65活化的作用,均存在剂量依赖。     

低浓度H2O2对HL-60细胞增殖的影响

目的探讨低浓度H2O2对同步化处理或未经处理的HL-60细胞作用的时间效应关系. 方法将培养的HL-60细胞进行同步化处理或取对数生长期细胞,施加不同浓度梯度的H2O2影响,作细胞计数,四唑盐（MTT）比色试验检测细胞生存率,流式细胞技术测定细胞周期分布. 结果低浓度H2O2作用组HL-60活细胞数明显高于未施加影响组,尤以H2O2终浓度为2 μmol*L-1组作用明显（P<0.01）,细胞周期也有轻度改变. 结论低浓度H2O2作用HL-60细胞可使细胞数增加,其影响有时间效应关系,机制可能同其影响细胞周期有关.     

辛伐他汀对急性早幼粒细胞白血病NB4细胞NF-κB信号通路的影响

目的 探讨辛伐他汀对人急性早幼粒细胞白血病细胞株(NB4细胞)增殖与凋亡的影响以及对凋亡信号转导通路NF-κB通路相关基因mRNA表达的影响,以探讨辛伐他汀抗白血病效应及可能机制.方法 以不同浓度辛伐他汀处理NB4细胞,取对数生长期细胞进行实验.实验分为阴性对照组和辛伐他汀处理组(终浓度分别为5、10、15 μmol/L),培养24、48、72 h.采用MTT法观察NB4细胞增殖能力;流式细胞术测定NB4细胞凋亡指标AnnexinⅤ/propidium iodide变化;PCR芯片研究NB4细胞NF-κB通路相关基因mRNA的差异表达.结果 辛伐他汀对NB4细胞的生长有明显抑制作用和促凋亡作用(均P＜0.01),且呈时间与剂量依赖性.15 μmol/L辛伐他汀处理NB4细胞24、48、72 h细胞抑制率分别达到45.75％、92.91％、96.46％,而细胞早期凋亡率分别为30.25％、64.34％、87.38％.与对照组相比,15 μmol/L辛伐他汀处理NB4细胞48 h组中NF-κB通路有56个基因表达发生改变,其中47个基因表达下调、9个基因表达上调.结论 辛伐他汀能抑制NB4细胞增殖并诱导其凋亡,其诱导凋亡机制可能与辛伐他汀调节NF-κB通路相关基因的表达有关.     

罗格列酮抗人HL-60细胞增生作用及其作用机制

目的 探讨罗格列酮(rosiglitazone)对人急性髓性白血病细胞系(HL-60)细胞增生抑制及作用机制.方法 MTT法观察对HL-60细胞增生的影响,流式细胞仪检测细胞周期分布和凋亡率,免疫组化和Western blot检测分析PPARγ、Bax、bcl-2、NF-κB蛋白表达状况.结果 罗格列酮对HL-60细胞具有明显生长抑制效应,抑制率最高达77%,且呈时间-效应、剂量-效应依赖关系;流式细胞检测结果提示:10～100μmol/L罗格列酮能抑制HL-60细胞增生;50、100μmol/L罗格列酮能促进HL-60细胞凋亡.免疫组化和Western blot检测发现:HL-60细胞表达PPARγ;罗格列酮作用HL-60细胞后,发生核转位现象; Bax表达增加, bcl-2 、NF-κB的表达减少.用PPARγ阻断剂GW9662作用于HL-60 细胞后PPARγ、Bax、bcl-2、NF-κB蛋白表达无变化.结论 罗格列酮能抑制HL-60细胞增生,诱导细胞凋亡,其作用机制可能与激活PPARγ途径,上调Bax下调Bcl-2 、NF-κB有关.     

姬松茸粗多糖对急性早幼粒细胞白血病细胞株HL-60细胞增殖、凋亡及转录因子NF-κB活性的影响

目的：研究姬松茸粗多糖对急性早幼粒细胞白血病细胞株HL-60细胞增殖、凋亡及转录因子NF-κB活性的影响并探索其诱导凋亡机制。方法：培养HL-60细胞,用不同浓度（1,2,5,10 mg/mL）姬松茸粗多糖诱导48 h后,采用WST-1法测定细胞增殖抑制;流式细胞术测定凋亡;免疫荧光法测定NF-κB P65蛋白核转运。结果：经不同浓度姬松茸粗多糖作用后,HL-60细胞存活率分别为90.0%,77.5%,47.5%,17.5%,与对照组（99.0%）比有统计学意义（P〈0.05）;5 mg/mL姬松茸粗多糖诱导48 h后,HL-60细胞早期凋亡率为52.4%,较对照组（6.0%）明显增加（P〈0.05）;免疫荧光显示NF-κB核转运较对照组减少。结论：姬松茸粗多糖可抑制HL-60细胞增殖,呈浓度-依赖性;能诱导HL-60细胞凋亡;诱导凋亡的作用与抑制NF-κB通路异常激活相关。     

丙戊酸钠联合三氧化二砷对HL-60细胞株的体外研究

目的:探讨丙戊酸钠(sodium valproate,SV)联合三氧化二砷(arsenous trioxide,As2O3)诱导HL-60细胞凋亡与bcl-2﹑bax mRNA之间可能的关系。方法:在对数生长期的HL-60细胞中分别加入不同浓度的SV和1.0μmol/LAs2O3,采用MTT比色法测定细胞生长抑制率,用Annexin V-FITC/PI双重标记经流式细胞仪检测细胞早期凋亡率,RT-PCR检测bcl-2,bax mRNA在细胞中的表达。结果:SV抑制HL-60细胞生长并促进其凋亡,SV联合1.0μmol/L As2O3对HL-60细胞的生长抑制及促凋亡作用更明显。SV单独或联合1.0μmol/L As2O3作用于HL-60细胞,bcl-2mRNA表达下降,bax mRNA表达增高。结论:SV与As2O3有协同作用,抑制HL-60细胞生长并诱导其凋亡,bcl-2﹑bax mRNA的表达变化可能参与了SV对HL-60细胞的促凋亡作用。     

白细胞介素6对HL-60细胞NF-κB p65活性的影响

目的研究白细胞介素6(interleukin-6,IL-6)对粒细胞白血病细胞系HL-60核因子-κB p65(NF-κB p65)活性的影响,以探讨IL-6在白血病发生、发展中的作用.方法指数生长期HL-60细胞置于96孔培养板内RPMI-1640培养液培养2 h后,分为A、B 2组.A组加PBS液继续培养3 h;B组分别加0.5、5、50 μg/L的IL-6继续培养3 h.用流式细胞仪 (FCM)检测HL-60细胞NF-κB p65的活化率.结果 IL-6浓度为5、50 μg/L时诱导的HL-60细胞NF-κB p65活化率分别为(16.81±2.73)%、(22.37±4.29)%,与对照组(8.44±2.20)%相比差异有统计学意义(P＜0.05);IL-6浓度在0.5 μg/L时NF-κB p65活化率为(8.69±1.61)%,与对照组相比差异无统计学意义(P＞0.05);3种IL-6浓度组间的NF-κB p65活化率比较差异有统计学意义(P＜0.05).结论 IL-6有诱导HL-60细胞NF-κB p65活化的作用,且存在剂量依赖趋势.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.