脂联素及氧化修饰低密度脂蛋白在子痫前期中的测定及意义

目的测定脂联素和氧化修饰低密度脂蛋白(oxLDL)在子痫前期中的变化,探讨其意义。方法采用酶联免疫吸附法测定子痫前期组及对照组血清脂联素及oxLDL水平。结果(1)子痫前期组与对照组孕妇血清脂联素比较虽未见显著差异,但子痫前期重度组、子痫前期轻度组、对照细依次呈下降趋势。(2)子痫前期组与对照组血清oxLDL比较,见子痫前期重度组升高,与对照组比较有显著差异(P<0.01);子痫前期重度组较子痫前期轻度组升高,两组比较有显著差异(P<0.05);子痫前期轻度组虽较对照组升高,但两组比较无显著差异。(3)将血清脂联素与oxLDL水平进行相关性检验,在子痫前期组两者呈负相关(r=-0.64,P<0.05)。(4)子痫前期重度组血清脂联素与收缩压、舒张压呈负相关(r=-0.49,P<0.05;r=-0.42,P<0.05)。结论脂联素和oxLDL可能是子痫前期发病的重要因素。     

MicroRNA及其在子痫前期中的作用研究进展

近年来有研究发现一些miRNAs在子痫前期患者的胎盘与正常妊娠胎盘组织中的表达存在显著性差异,推测miRNAs可能在胎盘生长发育过程中发挥了一定的作用,其失衡表达可能参与了子痫前期的发病过程。血清或血浆等体液中稳定存在的miRNAs有望作为妊娠性疾病早期预警诊断的理想的、潜在分子标记,而针对miRNAs设计分子靶向药物的探索为揭示子痫前期发病的机理、制定有效的个体化治疗方案具有重要意义。本文即对有关miRNAs及其在子痫前期发病中的最新研究进展作一综述。     

氧化修饰的低密度脂蛋白对内皮细胞的损伤和动脉粥样硬化

动脉粥样硬化 (ＡＳ)是一个多因素 ,多环节导致的疾病。虽然有关ＡＳ发生机理的各个学说各有侧重 ,如“脂质浸润学说”、“损伤反应学说”、平滑肌细胞“单克隆生长学说”、“氧化修饰脂蛋白学说”、“慢性增殖性炎症学说”等 ,但内皮细胞 (ＥＣ)损伤几乎贯穿于各个学说之中 ,被广泛认为是ＡＳ发生过程中最重要的始动环节。而高脂血症是ＡＳ公认的最重要的危险因子之一。近年的大量研究进一步表明[1～ 3 ] ,氧化修饰的低密度脂蛋白 (ｏｘ ＬＤＬ)在ＡＳ发生中的重要作用越来越被学者们重视和认同。本文将着重就ｏｘ ＬＤＬ损伤ＥＣ导致ＡＳ…     

印迹基因IGFⅡ和H19在子痫前期中的研究进展

子痫前期是导致围产期母儿死亡的主要原因,临床上缺乏有效的诊治方法。该病发病机制的核心是早期绒毛外滋养细胞的侵袭不良。基因组印迹与滋养细胞侵袭不良有关,是子痫前期未来研究的重要领域。胰岛素样生长因子(IGF)Ⅱ和H19是一对物理和机能连锁的印迹基因,它们参与滋养细胞的侵袭行为,综述印迹基因IGFⅡ和H19与子痫前期关系的研究现状,探索疾病的表观遗传学发病机制,有助于疾病的临床防治。     

剖宫产术在重度子痫前期中应用探讨（附124例分析）

目的探讨重度子痫前期时应用剖宫产术结束分娩为最佳分娩方式,以利母婴安全。方法对我院1996年1月至2006年12月收治的124例重度子痫前期孕产妇进行临床救治分析。结果剖宫产术114例,占91.93%,114例产妇中2例死亡,围产儿死亡8例,其中胎死宫内4例,4例早产儿。结论为改善母婴预后,适时选择剖宫产是重度子痫前期的最佳分娩方式。     

荧光定量PCR检测孕妇外周血中游离胎儿DNA在子痫前期中的价值

目的探讨孕妇外周血中的游离胎儿DNA水平作为子痫前期预测指标的可能。方法普通PCR检测子痫前期孕妇22例和正常孕妇25例的血浆中Y染色体性别决定基因（SRY）,然后应用荧光定量PCR技术对有SRY基因表达者的胎儿DNA水平进行定量分析并比较子痫前期孕妇组和正常孕妇组胎儿DNA水平的差异。结果22例子痫前期患者及25例对照组中各有12例出现SRY阳性信号。子痫前期组的胎儿DNA水平明显高于正常对照组（P〈0.01）。结论孕妇外周血中的游离胎儿DNA可望作为预测子痫前期的指标之一。     

子痫前期及子痫患者中期因子的表达及临床研究

目的:研究子痫前期及子痫患者的中期因子的表达及临床意义。方法:选取我院2009年1月-2010年12月产科住院的轻度子痫前期、重度子痫前期-子痫患者各60例,同期住院的正常妊娠晚期妇女60例,检测各组母体血清中期因子含量和记录孕产妇及围生儿并发症情况。结果:轻度子痫前期时母体血清MK表达明显升高,与正常妊娠晚期组相比较有统计学意义(P<0.01);重度子痫前期-子痫时MK表达明显降低,与正常妊娠晚期组、轻度子痫前期组相比较,有统计学意义(P<0.01)。三组母体及围生儿并发症相比较,有统计学意义(P<0.05)。结论:MK可作为疾病的监测和预后的重要指标,可成为该病治疗的新途径。     

氧化修饰低密度脂蛋白对人外周血单核细胞和THP-1细胞功能的影响

以天然低密度脂蛋白(nLDL) 为对照组, 探讨了氧化修饰低密度脂蛋白(oxLDL)对人外周血单核细胞(PBMs) 和人前单核白血病细胞株THP 1 粘附和分泌功能的影响。结果表明: oxLDL可上调单核细胞粘附分子CD11b 表达, 促进其与内皮的粘附, 并进一步诱导TNF α、IL 8 的分泌; 而nLDL无此作用。说明oxLDL是单核细胞粘附和激活的诱导剂, 经oxLDL诱导的THP 1 表现出与成熟单核 巨噬细胞相似的特征, 可作为体外动脉粥样硬化单核细胞分化激活的细胞模型     

氧化修饰低密度脂蛋白对人周血单核细胞和THP—1细胞功能的影响

以天然低密度脂蛋白（ｎＬＤＬ）为对照组，探讨了氧化修饰低密度脂蛋白（ｏｘＬＤＬ）对人外周血单核细胞（ＰＢＭｓ）和人前单核因病细胞株ＴＨＰ－１粘附和分泌功能的影响。结果表明：ｏｘＬＤＬ可上调单细胞粘附分子ＣＤ１１ｂ表达，促进其与内皮的粘附，并进一步诱导ＴＮＦ－α、ＩＬ－８的分泌；而ｎＬＤＬ无此作用。说明ｏｘＬＤＬ是单核细胞粘附和激活的诱导剂，经ｏｘＬＤＬ透导的ＴＨＰ－１表现出与成熟单核－巨噬细胞相似     

低密度脂蛋白的氧化修饰及其理化,生物学性质的改变

本文叙述了在体外对低密度脂蛋白（ＬＤＬ）进行氧化修饰的方式方法及修饰后ＬＤＬ的分子结构、理化性质和生物学性质的改变以及氧化修饰的ＬＤＬ在体内存在的证据等，说明ＬＤＬ的氧化修饰在动脉粥样硬化的发生发展中具有重要的病理生理意义。     

Resistance of native and circulating modified low-density lipoproteins in human blood to association

The resistance of native and circulating modified low-density lipoproteins from human blood to spontaneous and polyethylene glycol-induced association was studied by recording light transmission fluctuations. Circulating modified low-density lipoproteins were less resistant to association than native low-density lipoproteins. Polyethylene glycol-induced association of low-density lipoproteins was irreversible. Our results suggest that atherogenic activity of circulating modified low-density lipoproteins is associated with their increased predisposition to irreversible association.Translated from Byulleten Eksperimentalnoi Biologii i Meditsiny, Vol. 138, No. 10, pp. 428–431, October, 2004     

Resistance of native and circulating modified low-density lipoproteins in human blood to association

The resistance of native and circulating modified low-density lipoproteins from human blood to spontaneous and polyethylene glycol-induced association was studied by recording light transmission fluctuations. Circulating modified low-density lipoproteins were less resistant to association than native low-density lipoproteins. Polyethylene glycol-induced association of low-density lipoproteins was irreversible. Our results suggest that atherogenic activity of circulating modified low-density lipoproteins is associated with their increased predisposition to irreversible association.Translated from Byulleten Eksperimentalnoi Biologii i Meditsiny, Vol. 138, No. 10, pp. 428–431, October, 2004     

Cytotoxic effect of low-density lipoproteins

The cytopathogenic effect of various modified lipoproteins on cultured peritoneal macrophages was studied in vitro. After minor peroxide modification lipoproteins induced apoptosis of macrophages. Immune complexes containing oxidized lipoproteins caused cell necrosis.     

Antigenic differences between apo-B in native and circulating modified low-density lipoproteins

The state of apo-B in native and circulating modified low-density lipoproteins was studied by solid-phase enzyme immunoassay. We studied the interaction of these particles with monoclonal antibodies to apo-B of low-density lipoproteins. Native and circulating modified low-density lipoproteins had different affinity for the studied antigens. Our results illustrate conformational changes in apo-B of circulating modified low-density lipoproteins compared to native low-density lipoproteins. These changes probably contribute to increased accumulation of particles in vascular cells and their transformation into foam cells giving way to atherosclerotic vascular lesions. Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 138, No. 7, pp. 50–53, July, 2004     

Atherogenic lipoproteins found in the blood of patients with coronary atherosclerosis are desialylated low-density lipoproteins

Institute of Experimental Cardiology, All-Union Cardiologic Scientific Center, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR V. N. Smirnov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 110, No. 8, pp. 138–140, August, 1990.