bFGF mRNA 和蛋白在非小细胞肺癌表达与微血管密度测定的临床意义

目的 探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)碱性成纤维细胞生长因子(basic fibroblast growthfactor,bFGF)表达与测量微血管密度(microvessel density,MVD)的临床意义.方法 采用原位杂交和免疫组织化学SP法测定54例肺癌组织中bFGF表达和MVD,并根据病理类型、组织学分化程度、有无区域淋巴结转移以及预后进行分析.结果 在NSCLC中腺癌的bFGF表达阳性率和MVD值显著高于鳞癌(P＜0.05);所有NSCLC病例中,bFGF表达阳性率与肿瘤组织学分化程度有关,低分化癌bFGF表达阳性率显著高于中、高分化癌(P＜0.05),但MVD值与分化程度的关系不如bFGF明显;区域淋巴结转移阳性组NSCLC bFGF表达阳性率和MVD值显著高于阴性组(P＜0.01).结论 bFGF可能参与NSCLC的新血管生成并促进肿瘤转移;bFGF的检测可作为判断NSCLC转移及预后的指标之一.     

MCM5和Ki-67在乳腺癌组织中的表及意义

目的:探讨微小染色体维持蛋白5(MCM5)和Ki-67基因蛋白在乳腺癌组织中的表达及意义.方法:用免疫组织化学SP方法对手术诊断的142例乳腺癌组织与40例正常乳腺组织中MCM5和Ki-67基因蛋白进行检测,分析其表达情况.结果:MCM5、Ki-67在乳腺癌与正常组织中皆有表达,乳腺癌组织的表达明显高于正常组(P＜0.05).MCM5表达和Ki-67表达呈正相关(相关系数r=0.486P=0.031).MCM5表达与乳腺癌组织分级、临床分期、淋巴结转移有关(P＜0.05).Ki-67与临床分期、淋巴结转移有关(P＜0.05).结论:MCM5和Ki-67的表达与乳腺癌的发生、发展有关,可能成为新的乳腺癌肿瘤增生标志物.     

磷酸化AKT与NF-κB在非小细胞肺癌中的表达及临床意义

目的 探讨磷酸化AKT(p-AKT)与核转录因子-kB(NF-kB)在非小细胞肺癌(NSCLC)中的表达及临床意义.方法 应用免疫组织化学方法检测52例NSCLC组织和18例正常肺组织中p-AKT和NF-kB的表达.结果 p-AKT和NF-kB在NSCLC组织中的表达阳性率分别为53.8%和67.3%,均高于正常肺组织的0.0和16.7%(P＜0.05).p-AKT的表达与患者年龄、性别、吸烟、组织学类型、肿瘤组织分化程度及有无淋巴结转移、临床分期无关(P＞0.05).NF-kB的表达与淋巴结转移、临床分期有关(P＜0.05).p-AKT的表达与NF-kB表达呈正相关.结论 NSCLC组织中p-AKT、NF-kB高表达.p-AKT/NF-kB信号传导通路参与了NSCLC的发生发展,并与肿瘤转移和侵袭相关.     

Expressions and significance of SNCG and Ki-67 in gastric carcinoma

目的 研究乳腺特异基因BCSG1( SNCG)和Ki-67在胃癌组织中的表达及意义.方法 采用免疫组化法观察60例胃癌(A组)及20例正常胃黏膜石蜡(C组)标本中SNCG和Ki-67蛋白的表达.结果 A组SNCG和Ki-67的阳性表达率分别为65.0％和73.3％,均显著高手C组的0和20.0％ (P＜0.01).SNCG和Ki-67的表达与胃癌的浸润深度、淋巴结转移及肿瘤临床分期密切相关(P＜0.05或P＜0.01).且SNCG的表达和Ki-67的表达呈正相关(P＜0.01).结论SNCG和Ki-67在胃癌组织中显著高表达,与胃癌的浸润转移相关,可能共同参与胃癌的发生、发展过程.     

VEGF、MVD、Ki-67联合检测与大肠癌临床病理特征及预后相关性的研究

目的：探讨血管内皮生长因子（ vascular endo-thelial growth factor, VEGF）、微血管密度（ microvessel den-sity, MVD）和细胞增殖核抗原（ Ki-67）在大肠癌组织中的表达及预后意义。方法采用免疫组织化学检测64例大肠癌组织中VEGF、MVD和Ki-67的表达情况,并分析其与大肠癌临床病理特点和预后的关系。结果免疫组织化学检测显示VEGF、MVD、Ki-67表达在不同Dukes分期、淋巴结有无转移中存在明显差异（ P ＜0．05）,不同分化程度之间MVD表达存在差异（ P ＜0．05）,而三者的表达与性别、年龄、部位、大小无关（P＞0．05）,VEGF、Ki-67表达与MVD呈正相关,且VEGF、MVD表达与预后相关。结论 VEGF、MVD、Ki-67表达与Dukes分期、淋巴结转移、肿瘤分化程度相关,VEGF、MVD与预后相关,可作为检测大肠癌预后的参考指标。     

乳腺癌患者血清中IL-10含量和Ki-67表达相关分析及临床意义

目的:探讨浸润性乳腺癌患者血清中IL-10含量及Ki-67表达变化与与年龄、肿瘤大小、临床分期、淋巴结转移的关系以及它们相互之间的线性关系.方法:采用酶联免疫法检测癌患者血清中IL-10含量,采用免疫组化SABC法检测标本中Ki-67表达,并与患者临床病例因素进行分析.结果:患者血清中IL-10含量与患者年龄无关,但与肿瘤大小(P＜0.05),TNM分期(P＜0.01)以及有无淋巴结转移(P＜0.05)有关;Ki-67表达与患者是否发生淋巴结转移有关(P＜0.05);Ki-67高表达患者中IL-10含量较高(P＜0.05).结论:乳腺癌患者血清中IL-10含量与肿瘤恶性程度相关,其高表达提示患者顸后较差.     

VEGF、Ki67的表达与宫颈癌浸润、淋巴结转移关系的探讨

目的:探讨宫颈癌组织中VEGF、Ki67的表达与肿瘤浸润性及淋巴结转移的关系.方法:收集45例手术切除或活检标本,包括正常宫颈组织,宫颈鳞癌组织(按有无淋巴转移、分化及临床分期分组).进行SP免疫组化染色测定VEGF蛋白、Ki67蛋白的表达.结果:正常宫颈组织VEGF蛋白不表达,宫颈癌组织中VEGF的表达率为48.57%.VEGF阳性表达与分化程度、临床分期、淋巴结转移呈正相关(χ2＝10.3001,P＜0.01;Z.一10.1521,P＜0.0t;χ2＝5.9063,P＜0.05).Ki67阳性表达与临床分期、分化程度无正相关,与淋巴结转移呈正相关(χ2=2.6662,P＞0.05;χ2=0.8649,P＞0.05;χ2=11.9442,P＜0.01).结论:VEGF可能在宫颈癌的发生发展和袭转移中起重要作用,有望成为判断宫颈癌预后的指标.Ki67可作为反映宫颈癌细胞增殖能力的指标.     

血管内皮生长因子在非小细胞肺癌中的表达

目的 研究血管内皮生长因子(VEGF)在非小细胞肺癌(NSCLC)中的表达及其与肿瘤血管形成、临床分期、淋巴结转移的关系.方法 应用免疫组织化学方法和酶联免疫吸附试验(ELISA)检测56例NSCLC患者的肺癌组织和血清中VEGF的表达水平,并分析其临床意义.结果 NSCLC组织中VEGF的表达水平明显高于正常黏膜组织和癌旁组织(P＜0.05),且随临床分期Ⅰ、Ⅱ、Ⅲ～Ⅳ期的顺序明显上升(P＜0.05);同时有淋巴结转移的VEGF阳性率明显高于无淋巴结转移组(P＜0.05);NSCLC患者血清VEGF水平显著高于正常对照组(P＜0.01),并随肺癌临床分期Ⅰ、Ⅱ、Ⅲ～Ⅳ期的顺序明显上升(P＜0.05);淋巴结转移组患者血清VEGF水平明显高于无淋巴结转移组(P＜0.05).结论 VEGF在NSCLC组织中表达明显增高,并与NSCLC的发生、发展及其淋巴结转移有关,血清VEGF是NSCLC生长、转移的重要指标.     

非小细胞肺癌环氧化酶Ⅱ表达及意义

目的 探讨环氧化酶Ⅱ(COX-2)在非小细胞肺癌(NSCLC)组织中的表达及其与NSCLC发生、发展关系.方法 采用免疫组织化学法检测45例NSCLC组织和癌旁正常组织标本COX-2的表达水平.结果 COX-2在NSCLC组织的表达率为64.4%(26/45),明显高于癌旁正常组织的31.1%(13/45),两者比较差异有统计学意义(P＜0.05).COX-2表达在不同性别、年龄及组织分化间差异无统计学意义(P＞0.05);COX-2表达在腺癌明显高于鳞癌(P＜0.05),直径＞3 cm肿瘤组织明显高于直径≤3 cm肿瘤组织(P＜0.05),有淋巴结转移者明显高于无淋巴结转移者(P＜0.05),TNM分期Ⅲ+Ⅳ期者明显高于Ⅰ+Ⅱ期者(P＜0.05).结论 COX-2参与了肺癌发生、发展过程.     

COX-2与Ki-67在宫颈癌中的表达及其意义

目的:探讨环氧合酶-2(COX-2)与Ki-67在宫颈癌及正常宫颈组织的表达情况及其临床意义.方法:采用免疫组织化学法(SP法)检测COX-2与Ki-67在62例宫颈癌和20例正常宫颈组织中的表达.结果:宫颈癌组织COX-2的阳性表达与组织学类型、分化程度和淋巴转移相关(P＜0.05或P＜0.01),与临床分期无明显相关(P＞0.05);而Ki-67蛋白的阳性表达与宫颈癌的分化程度及淋巴转移有关(P＜0.05或P＜0.01),而与组织细胞学类型及临床分期无明显相关(P＞0.05).结论:COX-2与Ki-67的表达与宫颈癌的生长及转移密切相关.     

Degraded and undegraded carrageenans and experimental gastric and duodenal ulceration

The prevention of histamine-induced gastric and duodenal ulceration in the guinea-pig has been examined using a series of undegraded and degraded carrageenans. Undegraded carrageenans were active at lower doses than degraded carrageenans. The high viscosity of the undegraded carrageenans in solution prevented their use in larger doses. Degradation of carrageenan without serious loss of sulphate, gives a product which allows the dose to be increased to an extent that its effect more than offsets the slight loss in activity caused by the degradation. No single feature of carrageenan structure can be related to anti-ulcer activity although degradation, and hence reduction of molecular size, generally reduces activity. Sulphate contents over 30% have little apparent effect on activity; κ-carrageenans were not consistently different in anti-ulcer activity from Λ-carrageenans. This contrasts with the antipeptic activity of carrageenans where κ-carrageenans are less active than their Λ-counter-parts. As with antipeptic activity, the degree of anti-ulcer activity is probably determined by a combination of structural features which includes molecular size and polyanionic properties.     

Affective and Anxiety Disorders and Alcohol and Drug Dependence:

Depression and anxiety frequently coexist in patients with substance use disorders. This clinically-oriented article examiens the relationship between these conditions and emphasizes data showing that substances of abuse can cause signs and symptoms of both depression and anxiety. These substance-related syndromes appear to have a different course and prognosis than uncomplicated, independent anxiety and major depressive disorders, and clinicians should consider the role of alcohol and other drugs in all patients presenting with these complaints. The authors will also outline an approach for diagnosing and managing patients with the combination of a substance use and depressive or anxiety disorder.     

Alcohol and Substance Use and Abuse in Women and Children

No abstract available for this article.     

Glucosidase and fucosidase inhibitors and Advances in nucleosides and nucleotides.

The American Chemical Society Symposium "Glucosidase and fucosidase inhibitors" took place on 1 April 1998 and was organized by Professors Zbigniew J Witczak (UConn, School of Pharmacy, CT, USA), Kuniaki Tatsuta (Waseda University, Tokyo, Japan) and Waldemar Priebe, MD (Anderson Cancer Center, University of Texas, USA). Professor Witczak provided introductory remarks including the status of existing glucosidase inhibitors, and chaired the morning session, which consisted of six lectures. The symposium was well received, and was particularly attractive for those interested in networking, as attendance was about sixty. In addition, some participants and attendees presented posters on the subject during the regular poster session organized by the Division of Carbohydrate Chemistry.