促红素对重型颅脑损伤伴休克大鼠IL-1β及S-100β蛋白表达的影响

目的探讨促红素对大鼠重型颅脑损伤伴休克后继发性损伤的保护机制。方法采用改进Feeney法制作颅脑损伤模型，同时行股动脉放血造成休克模型，将70只雄性Wistar大鼠用随机数字表法分为干预组及对照组。双抗体夹心酶联免疫（ELISA）分析S-100β蛋白及IL-1β变化。结果大鼠重型脑损伤伴休克后，IL-1β呈高表达，S-100β表达也增高，二者存在正相关关系。促红素能够在一定程度上抑制IL-1β高表达。结论促红素可能通过抑制颅脑损伤后继发的过度炎症反应而减轻继发性脑损伤。     

亚低温对重型脑损伤后血清IL-1β和S-100β蛋白含量的影响

目的观察重型创伤性脑损伤(severe traumatic brain injury,sTBI)患者血清IL-1β和S-100β蛋白含量变化以及亚低温治疗对IL-1β和S-100β蛋白含量的影响,探讨亚低温脑保护的可能机制。方法46例sTBI患者随机分成常温治疗(normothermia-treated,NT)组和亚低温治疗(mild hypothermia-treated,HT)组,分别予以常温治疗和亚低温治疗。两组均于伤后6, 24 h、3,8 d等各时间点采用酶联免疫吸附(ELISA)法测定IL-1β和S-100β蛋白含量;分析各时间点两组IL-1β和S-100β蛋白含量与GCS的相关性。结果(1)伤后各时间点两组sTBI患者血清IL-1β和S-100β蛋白含量明显高于对照组(P<0.01),但两组间IL-1β和S-100β蛋白含量在伤后6 h差异无统计学意义(P>0.05)。(2)亚低温治疗后各时间点HT组IL-1β和S -100β蛋白含量低于NT组(P<0.01)。(3)NT组于伤后各时间点IL-1β和S-100β蛋白含量均与GCS呈负相关;HT组IL-1β和S-100β蛋白含量于伤后24 h、3 d与GCS无相关性,6 h、8 d与GCS呈负相关;与NT组比较,出院时HT组预后较好。结论亚低温治疗能够降低sTBI患者IL-1β与S-100β蛋白含量,改善预后,具有脑保护作用。其脑保护机制可能与亚低温能减轻血清IL-1β和S-100β蛋白介导的损伤性脑细胞炎症反应有关。     

S-100B蛋白评估重型脑损伤预后的临床研究

目的　研究重型脑损伤后血清S - 10 0B蛋白浓度变化 ,以期为重型脑损伤后原发性和继发性脑损害监测及预后评估提供直观的定量手段。　方法　对 2 0 0 2年 1月～ 2 0 0 2年 12月4 0例重型脑损伤住院患者伤后 12h～ 4d进行连续血清S - 10 0B蛋白浓度检测 ,并结合临床表现及格拉斯哥预后评分 (GOS)进行比较分析。　结果　本组 4 0例重型脑损伤患者伤后 12h血清S- 10 0B蛋白浓度均显著高于正常对照组 ,不同严重程度组之间S - 10 0B蛋白浓度差异存在非常显著性意义 (P <0 .0 1)。以伤后 12h血清S - 10 0B蛋白浓度 2 .0 0 μg/L为标准评估预后 ,其特异度为91% ,敏感度 72 % ,相对危险度 4 .33。本组 4 0例重型脑损伤患者伤后S - 10 0B蛋白浓度虽均呈下降趋势 ,但仍高于正常值 ,且预后恶劣组伤后各天S - 10 0B蛋白浓度均持续显著高于预后良好组 ,两组差异有非常显著性意义 (P <0 .0 1)。　结论　重型脑损伤后血清S - 10 0B蛋白浓度变化对原发性和继发性脑损害程度以及预后的评估有重要意义 ,为临床救治效果及病情转归的判断提供了有效手段。     

MgSO4对大鼠急性放射性脑损伤后NSE和S-100蛋白表达的影响

目的探讨MgSO4对电离辐射诱发的脑组织损伤的保护作用。方法将成熟的SD大鼠36只随机分为空白对照组、实验对照组和MgSO4实验用药组，用6MeV电子线对实验大鼠行20Gv全脑单次垂直照射，吸收剂量率为200cGy／min；实验用药组大鼠于照射前1d、照射后即刻、照后连续3d分别给予10％的MgSO4腹腔注射，分别于照射后1、7、14和30d处死大鼠，取其脑组织，用免疫组织化学法测定神经元特异性稀醇化酶（NSE）、S-100蛋白的相对含量，并与对照组进行比较。结果在上述时间点，脑内海马区S-100和NSE表达有时间规律，照射后1周S-100蛋白的表达和照射后24hNSE的表达组间存在一定差别。与空白对照组相比，实验对照组大鼠脑组织中NSE表达显著下降（P〈0．05），S-100表达显著升高（P〈0．05）；与实验对照组相比，在照射后7d，MgSO4可明显抑制S-100的表达（P〈0．05），诱导NSE的表达（P〈0．05）。结论 脑组织中S-100和NSE表达水平的变化是辐射诱导的急性脑损伤的敏感指标，MgSO4可降低S-100的表达水平并升高NSE在神经元中的含量，早期使用对急性放射性脑损伤有保护作用。     

亚低温治疗新生儿窒息后脑损伤临床疗效及对血清S100β蛋白影响

目的 探讨亚低温治疗新生儿窒息后脑损伤的临床疗效及对血清S100β蛋白的影响.方法 选取华北石油管理局总医院自2018年5月至2020年12月收治的84例窒息后中重度脑损伤患儿为研究对象.采用随机数字表法将其分为常规治疗组与亚低温治疗组,每组各42例.常规治疗组患儿给予对症支持治疗,如维持电解质平衡、降颅压、抗感染、静...     

TRH对失血性休克大鼠组织cAMP的影响

笔者观察了促甲状腺素释放激素（ＴＲＨ）对失血性休克大鼠在休和离体组织ｃＡＭＰ水平的影响，结果显示大鼠失血性休克发展到一定严重程度时，心室肌、肝组织和肾皮质ｃＡＭＰ水平明显下降。ＴＲＨ治疗可逆转这些改变，在普奈洛尔预处理的失血性休克大鼠，ＴＲＨ的这种作用消失。离体实验中，ＴＲＨ本身不影响肝组织ｃＡＭＰ水平，但能明显增加异丙肾上腺素刺激肝组织ｃＡＭＰ的产生，普奈洛尔可消除ＴＲＨ的这种作用。本研究结果…     

大鼠创伤性脑损伤合并失血性休克模型的建立

目的 建立一种大鼠创伤性脑损伤合并失血性休克的模型. 方法 采用自由落体撞击法联合静脉损伤法制作大鼠创伤性脑损伤合并失血性休克模型,并记录相关生理学参数.神经功能量表测量神经损伤评分,干湿重法测量脑水肿程度,伊文思蓝(EB)染色法测量血脑屏障(blood brain barrier,BBB)破坏程度,HE染色及免疫组织化学染色评估脑组织病理学改变. 结果 该模型造成大鼠血压在3 min内从95 mm Hg(1 mm Hg =0.133 kPa)降至25 mm Hg左右,1h后仍维持于60 mm Hg左右.神经损伤评分明显升高.脑组织含水量从77％左右上升至81％左右.脑组织残留伊文思蓝升高了1倍多.HE染色可见脑组织神经元皱缩、嗜酸性深染、细胞周围空泡等.免疫组织化学染色可见神经元β -淀粉样前体蛋白(β- amyloid precursor protein,β -APP)含量明显升高. 结论 成功模拟创伤性脑损伤合并失血性休克的大鼠模型,并复制出脑水肿、血脑屏障破坏、神经元受损、β - APP表达等主要病理变化.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

目的 通过动态观察亚低温对急性重型颅脑损伤患者血清S-100B蛋白浓度的影响,探讨亚低温在急性重型颅脑损伤治疗中的作用.方法 将120例急性重型颅脑损伤患者随机数字表法分为亚低温组和常规组.亚低温组在常规治疗的基础上,予亚低温治疗,直肠温度维持在33～35℃,持续3～5 d.所有患者于入院6 h内,入院后第2,3,4,5,6天动态检测血清S-100B蛋白浓度.3个月后对患者进行GOS评估.结果 亚低温组和常规组血清S-100B蛋白浓度明显高于正常对照组(P<0.05);亚低温组血清S-100B蛋白浓度明显低于常规组(P<0.05);亚低温能够改善急性重型颅脑损伤患者的预后.结论 早期应用亚低温能显著降低急性重型颅脑损伤患者血清S-100B蛋白浓度,保护神经功能,改善预后,其脑保护作用可能与亚低温能减轻S-100B蛋白介导的损伤性脑细胞炎症反应有关.     

+GZ暴露对大鼠脑热休克蛋白-70表达水平的影响

目的 探讨＋Ｇｚ暴露条件下大鼠脑中热休克蛋白－７０（ＨＳＰ７０）的变化及其在＋Ｇｚ所致脑损伤中的作用。方法 １００只大鼠,随机分为４胡,每组２５只,一组作为空白对照,其余三组分别进行＋２Ｇｚ,＋６Ｇｚ和１０Ｇｚ暴露,峰值作用时间３ｍｉｎ,加速度增长率１Ｇ／ｓ然后,分别于暴露后的６ｈ、１ｄ、２ｄ、４ｄ和６ｄ麻醉大鼠,心脏灌注后迅速取脑组织,用Ｗｅｓｔ ｂｌｏｔ法比较ＨＳＰ７０的变化情况。结果 ＋Ｇｚ暴露后６ｈＨＳＰ７０开始升高,１ｄ达到高峰,至６ｄ时基本恢复正常。不同＋Ｇｚ暴露之间进行比较可以看出,＋６ＧＧｚ组ＨＳＰ７０表达最高,＋１０Ｇｚ最少。结论 ＋Ｇｚ暴露对大鼠脑ＨＳＰ７０表达水平有显著的影响。     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

亚低温对急性重型颅脑损伤患者血清S-100 B蛋白的影响

Objective To dynamically observe the effect of mild hypothermia on concentration of plasma S-100B protein in patients with acute severe brain injuries so as to further explore its role in treat-ment of acute severe brain injury. Methods A total of 120 patients with acute severe brain injuries were randomly divided into mild hypothermia group and general group. The patients in mild hypothermia group were treated with mild hypothermia besides conventional therapy, with maintenance of rectal tem-perature at 33℃-35℃ for 3-5 days. Serial concentration of S-IOOB protein in serum was measured in all patients from 6 hours to 6 days after hospitalization. GOS evaluation was done three months after treat-ment. Results The concentration of S-100B protein in serum of mild hypothermia group and general group was significantly higher than of normal group (P <0.05), with significant lower level in mild hypo-thermia group than general group(P <0.05). Mild hypothermia could improve prognosis of patients with acute severe brain injury. Conclusions Early use of mild hypothermia can decrease concentration of S-100B protein in serum, protect neurofunction and improve prognosis, as may be related to its function in alleviating damnification brain cell inflammation reaction mediated by S-100B protein.     

重型颅脑创伤后脑疝伴失血性休克救治策略

目的 探讨脑疝形成伴失血性休克的重型颅脑创伤的早期救治策略及其对预后的影响.方法 回顾性分析我院2006年5月-2009年5月以"选择性早期颅脑手术"救治的54例脑疝伴失血性休克患者(研究组),并与2003年4月-2006年4月收治的未按该方法救治的48例同类患者(对照组)进行比较,比较患者1周内死亡率以及伤后半年的GOS评分.结果 两组患者性别、年龄、致伤机制、GCS、失血量等指标差异无统计学意义(P＞0.05).研究组54例患者1周内死亡13例,死亡率为24%;对照组患者1周内死亡16例,死亡率为33%(P＜0.05).研究组患者伤后半年GOS显示预后良好率较对照组高(P＜0.05).结论 按休克程度行选择性早期颅脑手术可以提高重型颅脑创伤后脑疝伴失血性休克患者的早期救治效果,改善患者的预后.