白细胞精子症病人精浆sIL—2R和HSPM水平变化探索

本研究旨在深入了解白细胞精子症病人精浆可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）水平和精浆免疫抑制物（ＨＳＰＩＭ）水平变化，并与正常生育组进行对比，结果发现白细胞精子症精浆中ｓＩＬ－２Ｒ水平明显高于正常生育或百白细胞精子症的不症对照组，而ＨＳＰＩＭ却明显低于各对照线；同时ｓＩＬ－２Ｒ水平和ＨＳＰＩＭ水平之间为负相关关系，结果提示，白细胞精子症精浆ｓＩＬ－２Ｒ水平升高时，男性生殖管道壁中淋巴细胞处于活     

白细胞精子症病人精浆可溶性白细胞介素2受体和精浆免疫抑制物水平？…

本文研究了白细胞精子症病人精浆ｓＩＬ－２Ｒ水平和ＨＳＰＩＭ水平变化。发现白细胞精子症精浆中ｓＩＬ－２Ｒ水平明显高于正常生育或非白细胞精子症的不育症对照组，而ＨＳＰＩＭ却明显低于各对照组；同时ｓＩＬ－２Ｒ水平和ＨＳＰＩＭ水平之间为负相关关系。结果提示：白细胞精子症精浆ｓＩＬ－２Ｒ水平升高时，男性生殖管道壁中淋巴细胞处于活化状态，它可能会引起白细胞大量进入精液，另外ＨＳＰＩＭ水平下降可能是引起淋巴细胞     

不育男性精浆IL—8水平的研究

本研究用双抗体夹心ＥＬＩＳＡ法检测７２例不育男性和２０例正常对照精浆白细胞介素－８（ＩＬ－８）水平。不育组ＩＬ－８水平明显高于正常对照组（Ｐ〈０．００１），其中，白细胞〉４个／ＨＰ精液组明显高于对照组（Ｐ〈０．００１），白细胞≤４个／ＨＰ精液组与对照线无明显区别（Ｐ〉０．０５）。白细胞〉４个／ＨＰ级 液中白细胞数与精浆ＩＬ－８呈正相关（ｒ＝０．８８３，Ｐ〈０．００１）。而且治疗前、后精浆ＩＬ－８浓     

白细胞精子症病人精浆IL-8、CRP水平变化

白细胞精子症 (ｌｅｕｋｏｓｐｅｒｍｉａ)是生殖系统感染后精液中出现大量白细胞所致。白介素 8(ＩＬ 8)能诱导多形核中性白细胞 (ｐｏｌｙｍｏｒｐｈｏｎｕｃｌｅａｒｎｅｕｔｒｏｐｈｉｌｌｅｕｋｏｃｙｔｅ ,ＰＭＮ)内钙离子浓度增高 ,并通过受体介导机制使ＰＭＮ趋向炎症灶。Ｃ 反应蛋白 (ＣＲＰ)是急性时相反应的一个极灵敏的指标 ,可激活补体 ,促进ＰＭＮ的运动和吞噬 ,并有调理素样作用。为探讨白细胞精子症ＩＬ 8、ＣＲＰ水平变化情况及其对判断炎症和创伤转归价值 ,我们测定了 38例白细胞精子症病人精浆ＩＬ 8及ＣＲＰ水平 ,报道…     

白细胞精子症病人精浆sIL-2R和HSPIM水平变化探索

本研究旨在深入了解白细胞精子症病人精浆可溶性白细胞介素-2受体（SIL－2R）水平和精浆免疫抑制物（HSPIM）水平变化，并与正常生育组进行对比，结果发现白细胞精子症精浆中SIL－2R水平明显高于正常生育或非白细胞精子症的不育症对照组，而HSPIM却明显低于各对照组；同时SIL－2R水平和HSPIM水平之间为负相关关系。结果提示，白细胞精子症精浆SIL－2R水平升高时，男性生殖管道壁中淋巴细胞处于活化状态，它可能会引起白细胞大量进入粮液。另外HSPIM水平下降可能是引起淋巴细胞活化的原因或是它在减少IL－2R表达过程中被消耗所致。     

白细胞精子症病人精浆可溶性白细胞介素2受体和精浆免疫抑制物水平变化探索

本文研究了白细胞精子症病人精浆ｓＩＬ－２Ｒ水平和ＨＳＰＩＭ水平变化。发现白细胞精子症精浆中ｓＩＬ－２Ｒ水平明显高于正常生育或非白细胞精子症的不育症对照组，而ＨＳＰＩＭ却明显低于各对照组；同时ｓＩＬ－２Ｒ水平和ＨＳＰＩＭ水平之间为负相关关系。结果提示：白细胞精子症精浆ｓＩＬ－２Ｒ水平升高时，男性生殖管道壁中淋巴细胞处于活化状态，它可能会引起白细胞大量进入精液，另外ＨＳＰＩＭ水平下降可能是引起淋巴细胞活化的原因或是它在减少ＩＬ－２Ｒ表达过程中被消耗所致。     

不育男性精浆IL－8水平的研究

本研究用双抗体夹心ＥＬＩＳＡ法检测７２例不育男性和２０例正常对照精浆白细胞介素－８（ＩＬ－８）水平。不育组ＩＬ－８水平明显高于正常对照组（Ｐ＜０．００１），其中，白细胞＞４个／ＨＰ精液组明显高于对照组（Ｐ＜０．００１），白细胞≤４个／ＨＰ精液组与对照组无明显区别（Ｐ＞０．０５）。白细胞＞４个／ＨＰ组精液中白细胞数与精浆ＩＬ－８呈正相关（ｒ＝０．８８３，Ｐ＜０．００１）。而且治疗前、后精浆ＩＬ－８浓度有明显差异，且与精液白细胞下降同步。ＩＬ－８是反映生殖系统感染的诊断及疗效判断的良好指标。     

白细胞精子症病人精浆活性氧与细胞因子检测及评价

目的 :研究不育症病人精浆中活性氧 (ROS)、超氧化物歧化酶 (SOD)及白介素 8(IL 8)的水平及其之间的关系。　方法 :收集 15例生育男性 (生育组 )、16例非感染性不育症者 (非感染组 )及 11例白细胞精子症者 (感染组 )的精液 ,进行常规精液分析 ,检测其精浆中IL 8、丙二醛 (MDA)、SOD及白细胞 (WBC)含量 ,并进行相关分析。　结果 :感染组与另两组相比 ,MDA、WBC及IL 8明显升高 (P <0 .0 0 1)。相关分析发现 ,IL 8与MDA显著相关 (r =0 .85 2 ,P<0 .0 0 1) ;WBC与IL 8间显著相关 (r=0 .818,P <0 .0 1)。　结论 :感染性不育症病人精液中 ,ROS与IL 8的增加有关 ,抗氧化系统缺陷是ROS增加的原因之一。     

生育男性和异常精子症病人精浆一氧化氮水平的差异

目的 :通过研究精子正常和异常男性精浆中一氧化氮 (NO)含量及动态的差异 ,以了解NO与男性生育力的关系。　方法 :采用硝酸还原酶法 ,经Greiss试剂显色后 ,用分光光度法测定 174例生育男性和 2 17例异常精子症男性精浆中NO的含量。　结果 :① 174例精子生育男性精浆中均检测出NO ,平均含量为 (2 7.78± 5 .81) μmol/L,并随年龄增加而增高 ,40岁以后增高显著。 2 0～ 2 9岁组 [(2 6 .2 5± 5 .5 2 ) μmol/L]与 30～ 39岁组 [(2 8.11± 5 .87)μmol/L]相比 ,差异无显著性 (P >0 .0 5 ) ,但 40～ 49岁组 [(30 .17± 6 .14) μmol/L]与 2 0～ 2 9岁组相比 ,增高有统计学意义 (P <0 .0 5 )。② 9类精子异常症男性的精浆中NO含量均显著高于精子正常组。增高幅度以精子单项异常为最小 ,以精子的 3项异常为最高 ,2项精子异常的精浆中NO水平介于两者之间。　结论 :男性精浆中适度的NO可能具有调节精子发生的作用。     

Evaluation of seminal plasma parameters in patients with chronic prostatitis or leukocytospermia

Summary. Though detailed cytological and microbiological diagnostic procedures are routinely carried out in male genital tract infection, the correct diagnosis and localization of inflammation or infection is often difficult. In this prospective study, the relevance of the seminal plasma markers PMN elastase, complement C3, CRP, fructose, PSP 94, PSA, and α-glucosidase was investigated in 13 patients with chronic prostatitis, 31 patients with significant leukocytospermia, and 58 patients with non-inflammatory diseases (controls). Statistically relevant results were obtained for PMN elastase when comparing chronic prostatitis with controls, leukocytospermia with controls ( P <0.001) and chronic prostatitis with leukocytospermia ( P <0.05); for complement C3 chronic prostatitis and leukocytospermia vs. controls ( P <0.05) and for fructose/ejaculate leukocytospermia vs. controls ( P <0.05). No statistically relevant differences were found for C-reactive protein, α-glucosidase, PSA and prostatic secretory protein (PSP 94). To delimit genital tract inflammation from non-inflammatory patients, cutpoint levels for PMN elastase of 230 ng ml⁻¹ and for C3c of 0.01 g l⁻¹ were suggested. PMN elastase was shown to possess the strongest discriminating power. The assessment of a cutpoint for fructose to indicate seminal vesicle dysfunction is not possible as the significance level is weak ( P <0.05).     

人精液中IL—8表达和白细胞数之间关系的研究

本研究采用过氧化物酶染色法检测３８０ 名男子射出精液中白细胞（ ＷＢＣ） 数量。其中４０ 名精液正常生育者中无一例精液ＷＢＣ增多；３４０ 例不育者中精液ＷＢＣ增多者有３６ 例，占１０．５９ ％ 。采用Ｗｅｓｔｅｒｎ Ｂｌｏｔ 和图象分析法检测２６ 名男子精浆中白介素８（ＩＬ８） 的表达量发现，不育组精液ＷＢＣ增多者其ＩＬ８ 表达量明显高于不育组精液ＷＢＣ未增多者和精液正常生育者（Ｐ＜ ０．０１），表明ＩＬ８ 的表达量与精液ＷＢＣ数呈正相关。     

Increased levels of interleukin-8 in human seminal plasma

Summary.  The role of cell-mediated immunity in the aetiopathogenesis of male infertility is far from being defined. The cytochemokine interleukin-8 (IL-8) has a key role in T-cell mediated immune responses. The aim of this study was to confirm the presence of IL-8 in human seminal plasma, to show differences between IL-8 concentrations in fertile and infertile subjects, and to show the potential relationship between IL-8 amounts in semen and spermiogram parameters. IL-8 levels were determined in the seminal plasma of 77 men divided as follows: (a) into seven groups according to the aetiological diagnosis of fertility and (b) into two groups on the basis of a normal or abnormal spermiogram. The mean value of IL-8 in the seminal plasma was 31.5 times higher than the upper limit in normal serum. There is a borderline statistical significant difference among the means of the various groups ( P <0.051). The Tukey's HSD test for multiple comparisons indicated no two groups as being significantly different, whereas the less conservative test LSD showed significant differences between the group with infection and groups with normal controls, Klinefelter's syndrome, mumps orchitis, cryptorchidism, or varicocele. There was no significant difference in IL-8 levels between men with normal and those with abnormal spermiograms. Furthermore, there was no correlation between IL-8 levels and the variables of the spermiogram. Even though the conclusions of this study have to be tempered by the sample size, IL-8 concentration in seminal plasma may be considered as a potential marker for the diagnosis of male accessory gland infection.     

白细胞精子症患者精液中一氧化氮和UA含量的关系分析

目的探讨白细胞精子症不育者精液中白细胞(WBC)密度、一氧化氮(NO)和尿酸(UA)之间的关系。方法依据WHO诊断标准,选择白细胞精子症不育者40例,非白细胞精子症不育者35例,生育者30例,采用过氧化物酶染色法进行精液WBC密度计数；采用镀铜镉还原荧光法检测NO代谢产物硝酸盐(NO_3)；UA含量测定采用尿酸酶。过氧化物酶偶联法。结果白细胞精子症组精液NO含量为(106．95±4．13)μmol/L、WBC为(1．985±0．696)×10~9/L,而UA含量(166．9±68．1)μmol/L,生育组NO含量(41．31±3．67)μmol/L、WBC为(0．038±0．024)×10~9/L和UA含量为(398．6±52．3)μmol/L。NO和WBC显著高于对照组,UA显著低于对照组(P＜0．01),UA与NO及WBC均呈显著性负相关(r=-0．795,P＜0．01：r=-0．857,P＜0．01)。结论白细胞精子症患者精液NO产生增多致UA含量下降,使精子中毒受损。提示临床在治疗时应加用抗氧化药物,可提高疗效。     

Relationships between seminal plasma arginase activity and spermatological parameters in rams

This study was conducted to evaluate the correlation between seminal plasma arginase activity and spermatological parameters in rams. In this study, five fertility-proven Awassi rams were used as material. Six ejaculates were collected from each ram by an artificial vagina. Spermatological parameters (semen volume, mass activity, sperm motility and concentration and abnormal sperm rate) were immediately determined in each ejaculate. For enzyme assay, the semen samples were centrifuged and stored at -20 °C for the analysis of arginase activity. The average seminal plasma arginase activity was 0.61 ± 0.20 U (mg protein)(-1) . There was a positive correlation between arginase activity and semen volume (r = 0.412, P < 0.05), semen mass activity (r = 0.610, P < 0.01), sperm motility (r = 0.447, P < 0.05) and sperm concentration (r = 0.808, P < 0.01). However, there was a negative correlation between arginase activity and abnormal sperm rate (r = -0.424, P < 0.05). In conclusion, this study clearly suggests that there is a significant correlation between seminal plasma arginase activity and spermatological parameters. In light of these results, seminal plasma arginase activity may be a biochemical criterion for determining sperm quality besides classical semen analysis parameters in rams.     

Melatonin and aromatase stimulating activity of human seminal plasma

Melatonin concentrations and aromatase stimulating activity were determined in human seminal plasma and correlated with sperm density and motility. Aromatase stimulating activity was determined with an in vitro rat granulosa cell system and melatonin by radioimmunoassay. Compared to normal semen, aromatase stimulating activity was lower in azoospermic individuals, while melatonin was higher in oligospermic and azoospermic samples. Aromatase stimulating activity correlated positively with sperm concentrations and a negative correlation was found between melatonin and sperm progression. These findings suggest that low sperm production is associated with low aromatase stimulating bioactivity in seminal plasma; and melatonin may have an effect upon both sperm production and motility.