高效液相色谱检测血中氟西汀浓度

以氯丙咪嗪为内标，用ＨＰＬＣ方法测定抗抑郁剂氟西汀的血药浓度。血清经碱化，用１．５％异丙醇的正庚烷抽提，使用Ｃ１８柱，在２３０ｎｍ进行色谱分析。批内批间的相对标准偏差小于４．３２％，最低检测３０ｎｍｏｌ／Ｌ．对４例服用氟丁汀的病人进行血浓检测，表明本法灵敏，快速，适于血浓监测和药动学研究。     

高效液相色谱法测定血清氯氮平药物浓度

目的 建立一种简便、快速、灵敏的反相高效液相色谱(HPLC)紫外检测法测定血清氯氮平药物浓度.方法 选用C18柱,波长254nm,移动相为甲醇∶乙腈∶水∶正丁胺∶冰乙酸(16∶36.5∶47.5∶0.5∶1,调PH8.0),流速1.0ml/min,以乙醚为萃取液.结果 氯氮平保留时间为(3.74±0.02)min,日内...     

高效液相色谱分析法检测培高力特血药浓度

目的 :建立检测培高力特血药浓度的高效液相荧光色谱分析法。方法 :用氯仿抽提法进行样本预处理。采用WatersC18柱( 4 6mm× 2 5 0mm ,5 μm ) ,以乙腈 /氯化铵缓冲液 ( 96/4 )为流动相。样品荧光检测发射光波长 2 85nm ,吸收光波长 3 45nm ,内标为盐酸地尔硫。结果 :培高力特在 1～ 8ng·mL-1呈良好的线性关系。血浆样品回收率可达 70 %。结论 :本法灵敏、准确、重现性好。     

高效液相色谱法测定血清硝基安定浓度的方法研究

目的 建立知于临床普遍应用的血清硝基安定浓度的测定方法。方法 应用反相高效液相色谱法,以茶碱为内标,采用窄径柱ＬＵＮＡＣ１８（２）分离,流动相为水和甲,二极和 列上检测器选择各组分最大吸收波长检测。结果 在４￣２５６μｇ／Ｌ范围内,硝基安定和内标的色谱峰高比与浓度呈线性关系;平均回收率为１０２．８％,变异系数为４．７％。最低检测浓度为４μｇ／Ｌ。对１８例癫痫患者进行治疗药物监测,取得良好疗效。结论     

高效液相色谱法检测血浆左旋肉碱方法的建立

目的:建立测定血浆左旋肉碱的高效液相色谱方法。方法:采用KromasilSil色谱柱(150mm×4.6mm,5μm),Agilent1100系列高效液相色谱系统,用衍生化的方法处理血样,绘制标准曲线,并对80名正常人及12例脂肪累积性肌肉病(LSM)患者进行血浆左旋肉碱检测。结果:本方法的回归方程为Y=0.9908C 0.6292,r=0.9999。精密度RSD<4%、加样回收率>70%。稳定性良好。正常男性血浆肉碱平均(50±9.4)μmol·L-1,女性(38±7.6)μmol·L-1。结论:本方法灵敏、准确,适用于临床血浆左旋肉碱浓度的监测。     

脑肿瘤间质化疗BCNU缓释微球的载药量高效液相色谱测定

目的建立体外磷酸盐缓冲溶液(PBS)聚乳酸/羟基乙酸(PLGA)缓释卡氮芥(BCNU)微球药物浓度的高效液相色谱分析方法,并用此法研究缓释微球的体外释放动力学特征。方法采用依利特HypersilODS2C18色谱柱(5μm,4.6mm×150mm),以甲醇:水(5050)为流动相,流速1.0ml/min,紫外光检测波长237nm。结果BCNU在0.005~0.35μmol/ml范围内呈线性(r=0.9992),最低检测下限为0.005μmol/ml。采用低、中、高浓度(0.03、0.1、0.3μmol/ml)的方法回收率分别为103.33%、101.43%和102.04%,日间及日内相对标准偏差(RSD)分别<4%和<1%。体外药物释放动力学研究表明,PLGA缓释BCNU时间可达3w以上。结论本法准确可靠,操作简便,适用于缓释BCNU微球的体外药物释放动力学研究。     

高效液相色谱法检测血清帕罗西汀药物浓度

目的建立一种简便、快速、灵敏的反相高效液相色谱(HPLC)紫外检测法测定血清帕罗西汀药物浓度。方法选用C18柱，波长295nm，丙咪嗪为内标，移动相为甲醇：水：三氯甲烷：异丙醇：四甲基乙二胺：冰乙酸=282：140：1．6：0．8：1．6：2，流速1．0ml／min，以甲苯、正己烷(1：4)为萃取液。结果帕罗西汀和内标的保留时间分别为3．40min和4．20min，批内和批间平均回收率各为98．4％～100．0％和98．8％～101．8％，RSD分别小于8．0％和5．1％，最低有效检测浓度为3ng／ml。结论本法操作简便、快速而精确，具有一定的实用价值。     

HPLC测定氯丙咪嗪和及其代谢产物血浓度

用反相 HPLC 紫外检测法同时测定血中氯丙咪嗪及其主要活性代谢物 N-去甲氯丙咪嗪浓度,批内和批间回收率分别>97.99%和99.37%,CV 分别<3.62%和2.66%,最低有效检测浓度为66.5nmol/L。本法操作简便,全部采用国产试剂,适合于常规检测和科研应用。     

高效液相色谱法检测氯丙嗪血药浓度

目的　建立反相高效液相色谱法检测抗精神病药氯丙嗪血药浓度。方法　采用惠普 110 0型高效液相色谱仪 ,原装进口BetaBasic C18分析柱 ,检测波长为 2 5 4nm ,柱温 4 0℃ ,甲醇、磷酸铵缓冲液为移动相 ,流速 1ml/min ,乙醚提取处理样本。以安定为内标。结果　批内、批间试验 :RSD <8 9% ;回收率 :92 0 %～ 99 4 %。最低检测限 :10ng。线性方程 :Y =3 9+132 9X ,r =0 9989,线性范围 :2 5～ 75 0ng/ml。结论　该方法快速、简便、正确 ,适合临床药物治疗时血药浓度的监测。     

The DNA content of cerebral cortex neurons. Determinations by cytophotometry and high performance liquid chromatography

The boundaries and relative sensitivities of the substrates of septal and cortical brain stimulation reward were mapped in relation to the dopamine terminal fields in these regions using a dorsal-ventral moveable electrode. Brain stimulation was rewarding at all levels of the posterior lateral septum and not just in the region of dopamine terminal innervation. Reward thresholds, ease of training, maximum response rates and stability of responding were all unrelated to the proximity of the stimulating electrode to the band of dopamine terminals revealed by glyoxylic acid-induced dopamine fluorescence. Stimulation was also rewarding in the anterior lateral septum; the best sites were in the ventral portions of this region although dopamine terminal fluorescence was uniform throughout. Thus the anatomy of the brain stimulation reward substrate of the lateral septal nucleus does not bear a special relation to the anatomy of dopamine terminals within this region. Stimulation was also rewarding in each of the dopamine terminal fields of the cerebral cortex. The best self-stimulation was obtained with electrodes in the medial frontal cortex; sulcal frontal cortex was next best, entorhinal cortex was next, and pyriform cortex, though reliably positive, supported the weakest self-stimulation. Variations in self-stimulation threshold were seen as electrodes were moved through homogeneous regions of dopamine terminal density in some regions, while stable thresholds were associated with movements through areas of varying dopamine terminal density in others; thus, again, there was no special relation between goodness of self-stimulation and density of dopaminergic innervation. These data suggest that rewarding brain stimulation in these regions is not due to direct activation of either the dopaminergic terminals or the cells that they innervate.     

Analysis of platelet phospholipids by high performance liquid chromatography

Analysis of platelet phospholipids was attempted by means of high performance liquid chromatography (HPLC). In order to separate phosphatidic acid (PA) in addition to phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE) and phosphatidylcholine (PC), the mobile phase of Chen and Kou (J. Chromatogr., 227, 25-31, 1982) was modified. For the quantitative analysis, fluorescein was found to be a suitable internal standard. With these inventions, the amount of phospholipids could be determined within 30 min after lipid extraction and 10(7) platelets were required for one assay. Then, stimulus linked phospholipids breakdown was studied using HPLC and the results were compared with those by conventional 32P-thin layer chromatography (TLC) method. Similar results were obtained except that the amount of PA determined by TLC was much higher, probably due to active phosphorylation process. These observations suggest that the quantitative analysis of phospholipids could be achieved by our method with HPLC, which is advantageous over conventional methods in rapidity, no requirement of isotopes and determination of absolute amount of phospholipids.     

Resolution of the stereoisomers of baclofen by high performance liquid chromatography

The GABA analogue baclofen [3-(p-chlorophenyl)-4-aminobutanoic acid] has stereospecific actions on the peripheral and central nervous systems. This paper describes the resolution of tritium-labelled baclofen by high performance liquid chromatography on a reverse-phase C18 column using a chiral mobile phase. The method, which may have general application to certain other GABA analogues, affords optically pure (+)- and (-)-baclofen labelled with tritium to high specific activity suitable for ligand binding and other neurochemical studies.     

胶质瘤细胞耐药性逆转的HPLC法研究

目的　检测胶质瘤细胞中阿霉素 (ADM )的含量 ,探讨抗P 糖蛋白 (P GP)单克隆抗体对细胞耐药性的逆转效果。方法　将胶质瘤细胞分为敏感组、耐药组、耐药 +抗P GP单抗组及空白对照组 ,采用高效液相色谱 (HPLC)法测定细胞中ADM含量。色谱条件为LiChrosorbC18柱 (2 5 0mm× 4 .6mm ,10 μm) ;流动相为甲醇 乙腈 0 .0 1mol·L-1磷酸二氢胺 冰醋酸 (5 0 2 2 2 8 0 .5 ) ;流速为 1ml·min-1;激发波长为 4 95nm ,发射波长为 5 6 0nm。结果　测得细胞内ADM浓度在 0 .1～ 1.0mg·L-1范围内呈线性关系 ,相关系数为 0 .9983。ADM在各种细胞中的浓度分别为 空白对照组 (0 .4 96± 0 .0 15 )mg·L-1,敏感组 (0 .5 33± 0 .0 2 9)mg·L-1,耐药组 (0 .5 0 2± 0 .0 75 )mg·L-1,耐药 +抗P GP单抗组 (0 .5 77± 0 .0 86 )mg·L-1。结论　HPLC法是一种简便而准确地检测细胞中ADM的方法。结果表明 ,与正常对照组相比 ,耐药的胶质瘤细胞中ADM浓度降低 ,加抗P GP单抗逆转后的细胞中ADM浓度升高 ,提示抗P GP单抗的确对胶质瘤细胞的耐药性有逆转作用     

Ontogeny of the metencephalic, mesencephalic and diencephalic content of catecholamines as measured by high performance liquid chromatography with electrochemical detection

Developmental changes in norepinephrine (NE), dopamine (DA) and epinephrine (E) contents of the rat metencephalon, mesencephalon and diencephalon, have been measured by high performance liquid chromatography with electrochemical detection, from fetal stages (E15 or E17 to E21) to postnatal days (P0 to P30) and compared to the adult levels. The data show a biphasic pattern in NE changes of the three brain areas, with a first increase in the late prenatal period, followed by a further development from P0 to P18, thus reaching the adult levels. A similar pattern of development is found for the mesencephalic and diencephalic DA contents. The E levels of the diencephalon are very low in comparison to the NE and DA concentrations, but present a gradual increase from E17 to P18. The results correlate with the development of catecholamine systems in brain area as measured by other methodological approaches.     

Simultaneous measurement of various antidepressants in the plasma of depressed patients by high performance liquid chromatography

A simultaneous analytical method was reported for measuring the plasma levels of amitriptyline, imipramine, clomipramine, maprotiline, nortriptyline, desipramine, desmethylclomipramine, desmethylmaprotiline and amoxapine by high performance liquid chromatography (HPLC). The total plasma levels of each parent drug plus its desmethyl metabolite were monitored in 29 depressed patients administered with amitriptyline, maprotiline or amoxapine using the present analytical method. There were significant linear correlations between the dose per kg body weight and the total plasma levels with amitriptyline and maprotiline, but no such correlation was found with amoxapine. The ratios of total plasma levels to dose per kg body weight of these three drugs were lower in outpatients than in inpatients. These results indicate that the monitoring of plasma levels of antidepressants is useful in treating depression.