肿瘤坏死因子基因多态性与溃疡性结肠炎相关性研究

目的 检测肿瘤坏死因子（TNF）α基因多态性是否与浙江地区汉族溃疡性结肠炎（UC）患者遗传易感相关.方法 血样来自浙江地区110例UC患者及292例健康对照者.通过特定引物的聚合酶链反应（PCR-SSP）方法直接检测野生型及TNF基因的6个多态性（TNF-1031T/C,-863C/A,-857C/T,-380G/A,-308G/A,-238G/A）.结果TNF-308A与汉族UC显著相关,等位基因频率在患者中为14.6%,在健康对照者中为8.9%,两者间差异有统计学意义（P=0.02）.TNF-857T的携带率在UC患者中为17.3%,健康对照者为12.2%,两者间差异无统计学意义（P=0.06）.单倍体分型显示,6种单倍体包括H5和H3,这两种单倍体都包含TNF-308A.H5单倍体频率在患者中为12.3%,在健康对照者为7.5%,两者间差异有统计学意义（P=0.03）.我们还发现了既往在白种人中较少见的单倍体H3.单倍体H4是由TNF基因启动子区域野生型等位基因组成,它的纯合子UC患者共有27例（24.5%）,在健康对照者中有102例（34.9%）,差异有统计学意义（P＜0.05）,说明野生型等位基因可能与疾病的发生无关.结论 TNF-308A可能与汉族UC患者遗传易感相关.进一步的功能研究有助于明确突变型TNFα在UC发病过程中的作用.     

老年冠心病患者肿瘤坏死因子基因多态性的研究

肿瘤坏死因子 (ＴＮＦ)是一种具有多种生物效应的前炎症细胞因子。多数研究证实冠心病 (ＣＨＤ)患者为多基因发病机制。在ＣＨＤ患者中 ,ＴＮＦ基因多态性的作用尚不清。因此 ,我们对ＣＨＤ患者ＴＮＦ基因多态性的分布状况 ,探讨ＴＮＦ基因多态性在ＣＨＤ发病机制中的作用及临床意义。1　资料与方法1.1　研究对象　选择 1999年 3月至2 0 0 0年 10月符合ＷＨＯ的诊断标准 (3例经冠状动脉造影证实 )住院的老年人ＣＨＤ患者 40例 ,男 3 6例 ,女 4例 ,年龄 60～ 85岁 ,平均 69 3岁。其中急性心肌梗死 1例 ,不稳定型心绞痛 2 3例 ,稳定型心绞痛 1…     

肿瘤坏死因子α基因多态性与肺结核易感性的相关性研究

目的检测肿瘤坏死因子α（tumer necrosis factor-α,TNF-α）基因启动子区rs1799724位点多态性,探讨该多态性与肺结核（pulmonary tuberculosis,PTB）易感性的关系。方法采用病例对照研究,选择302例肺结核病人和302例健康人群为研究对象,应用扩增阻滞突变系统聚合酶链反应（amplification refractory mutation system,ARMS-PCR）方法对肿瘤坏死因子α因子rs1799724多态性位点进行基因分型,用SPSS统计软件分析rs1799724位点多态性与肺结核易感性的关联。结果 TNF-α基因rs1799724多态性在两组人群中的频率分布无统计学意义,P〉0.05。结论 TNF-α基因rs1799724多态性与中国汉族人群对肺结核易感性无相关性。     

肿瘤坏死因子-β基因多态性与胃癌和十二指肠溃疡

目的研究胃癌和十二指肠溃疡患者中的肿瘤坏死因子（TNF）-β基因多态性，并分析TNF-β基因多态性与幽门螺杆菌（H.pylori）感染相互作用与胃癌和十二指肠溃疡的关系。方法采用PCR-RFLP方法对124例胃癌、83例十二指肠溃疡及96例健康对照者进行基因多态性分析。用ELISA方法检测血清Hp抗体。结果胃癌组TNF-β＋252＊G基因频率明显低于对照组（51．2％对63．0％，P＜0．05），TNF-β＋252＊A基因频率明显高于对照组（48．8％对37．0％，P＜0．05），TNF-β＋252A／A基因型的优势比值为2．44（95％CI 1．18—5．03）。在血清H．pylori抗体阳性的胃癌组中，这种基因多态性的影响表现得更明显，优势比值为2．52。十二指肠溃疡和对照组基因频率比较差异无统计学意义（P＞0．05），十二指肠溃疡组的血清H.pylori抗体阳性率明显高于健康对照组（P＜0．01）。结论中国汉族胃癌病人与TNF-β基因多态性相关联，TNF-β＋252A／A基因型与胃癌的易感性有关；十二指肠溃疡病人与TNF-β基因多态性无关联。H．pylori感染是十二指肠溃疡的独立危险因素。     

肥胖高血压病患者肿瘤坏死因子基因多态性

目的探讨肿瘤坏死因子-α(TNFα)基因多态性与肥胖高血压病患者体脂分布、血压、脂代谢和胰岛素敏感性的关系。方法对上海地区汉族人中218例超重或肥胖的高血压患者,195例正常体重、正常血压受试者,用PCRRFLP方法检测TNFαG308A,G238A,TNFαA252G多态的基因型,观察各基因型与肥胖高血压病患者体脂分布、血压、脂代谢和胰岛素敏感性的相关性。结果(1)肥胖高血压病组与正常对照组之间,各等位基因和基因型频率无明显差异;(2)各基因型之间,血压水平无明显差异;(3)但在肥胖高血压病组中,TNFαG308AGA+AA基因型者的腰围、空腹胰岛素水平、胰岛素敏感指数均明显高于GG型纯合子,分别为(95.23±8.41vs93.21±8.11)cm,(P<0.05);(8.74±2.31vs7.02±2.36)μIU/mL,P<0.05;(2.20±1.31vs1.68±1.28,P<0.05)。(4)不同基因型血清TNFα浓度无明显差异。结论TNFαG308A,G238A,TNFαA252G的多态性与上海地区汉族人肥胖相关的高血压可能无关,但TNFαG308A基因型可能具有与肥胖相关的胰岛素抵抗的遗传易感性。     

肿瘤坏死因子α基因多态性与慢性乙型肝炎病毒感染的相关性

乙型肝炎病毒（HBV）感染后临床表现的多样性，除与病毒因素有关，还与宿主的遗传因素密切相关。细胞因子在宿主清除病毒的免疫应答中发挥着重要作用，其基因多态性可影响细胞因子的整个转录，翻译和分泌过程，导致不同人群中细胞因子水平的差异，从而影响HBV感染后的转归。肿瘤坏死因子（TNF）α基因启动子区存在有多个多态性位点，分别为-1031（T／C）、-863（C／A）、-857（C／T）、-376（G／A）、-308（G／A）、-238（G／A）和-163（G／A）。     

肿瘤坏死因子基因多态性与慢性萎缩慢胃炎和胃腺癌的相关性

研究发现 ,肿瘤坏死因子 (ＴＮＦ)基因多态性与ＴＮＦ的分泌有关[1] ,甚至可调节其转录水平[2 ] 。ＴＮＦ基因多态性是否与人类某些肿瘤、自身免疫性疾病、感染性疾病相关 ,国外学者的研究尚无定论。迄今为止 ,国内外有关ＴＮＦ基因多态性与慢性萎缩性胃炎和胃癌关系的报道极少。因此 ,我们对湖北地区汉族人群ＴＮＦ α和ＴＮＦ β基因多态性分布与慢性萎缩性胃炎和胃腺癌的相关性进行了分析 ,以阐明ＴＮＦ基因多态性在慢性萎缩性胃炎和胃腺癌中的作用。一、对象随机选取健康体检者 16 4例 ,其中男 113例 ,女 5 1例 ,平均年龄 (5 2 3± 11 …     

肥胖高血压病患者肿瘤坏死因子基因多态性

目的探讨肿瘤坏死因子-α(TNF-α)基因多态性与肥胖高血压病患者体脂分布、血压、脂代谢和胰岛素敏感性的关系.方法对上海地区汉族人中218例超重或肥胖的高血压患者,195例正常体重、正常血压受试者,用PCR-RFLP方法检测TNF-α G-308A, G-238A, TNF-α A252G多态的基因型,观察各基因型与肥胖高血压病患者体脂分布、血压、脂代谢和胰岛素敏感性的相关性.结果 (1)肥胖高血压病组与正常对照组之间,各等位基因和基因型频率无明显差异;(2)各基因型之间,血压水平无明显差异;(3)但在肥胖高血压病组中,TNF-α G-308A GA+AA基因型者的腰围、空腹胰岛素水平、胰岛素敏感指数均明显高于GG型纯合子,分别为(95.23±8.41 vs 93.21±8.11)cm,(P<0.05);(8.74±2.31 vs 7.02±2.36)μIU/mL,P<0.05;(2.20±1.31 vs 1.68±1.28,P<0.05).(4)不同基因型血清TNF-α浓度无明显差异.结论 TNF-α G-308A,G-238A,TNF-α A252G的多态性与上海地区汉族人肥胖相关的高血压可能无关,但TNF-α G-308A基因型可能具有与肥胖相关的胰岛素抵抗的遗传易感性.     

老年冠心病患者血浆中肿瘤坏死因子及其基因多态性的研究

目的　对老年冠心病患者血浆肿瘤坏死因子 (TNF)水平及其基因多态性进行分析 ,探讨其在冠心病发病中的作用。方法　用ELISA及PCR法测定 40例冠心病患者及 30例健康对照者血浆TNF水平及TNF等位基因频率。结果　TNF等位基因频率分布 ,两组之间比较差异无显著性意义 (P >0 .0 5 ) ,而血浆中TNF水平 ,冠心病组明显高于对照组 ,两组之间比较差异有显著性意义 (P <0 .0 0 1) 。结论　TNF基因多态性在冠心病发病机理中可能不起重要作用。TNFA或TNFB基因多态性与冠心病患者血浆TNF水平升高没有关系。     

炎症性肠病与肿瘤坏死因子基因多态性的研究进展

炎症性肠病包括溃疡性结肠炎与克罗恩病,病因至今未明,可能与肿瘤坏死因子相关,后者受其基因多态性影响.肿瘤坏死因子的等位基因频率,基因型频率和基因携带率影响炎症性肠病的疾病易感性与临床表现,存在种族与区域差异,对疾病的发展和预后产生重要作用.     

TNFα-308和LTα基因多态性与胃癌预后的关系

目的 研究肿瘤坏死因子α（tumor necrosis factor alpha，TNFα）308位点和淋巴毒素α（1ymphotoxin α，LTα）基因多态性与胃癌预后的相关性。方法 采用多聚酶链反应限制性片断长度多态性方法，检测56例胃癌患者及271例健康对照者TNFα和LTα基因型与等位基因频率。对随访的30例行基因型与1年和3年生存率的相关分析。结果 TNFα-308、LTαNeoI、AspHI基因型与胃癌和胃癌患者1年和3年生存率均无显著相关性。结论 TNFα-308、LTαNeol、AspHI基因多态性与中国湖北汉族人胃癌预后无关。     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

目的 研究我同湖北汉族人群IL-10及TNF的基因多态性与胃十二指肠疾病及幽门螺杆菌(Hp)感染的关系.方法 采用病例财照研究和聚合酶链反应.限制性片段长度多态性(PCR-RFLP)方法检测605例胃十二指肠疾病患者(包括196例慢性胃炎、189例胃十二指肠溃疡及220例胃癌患者)和624例健康对照者中IL-10基因启动子区3个位点(IL-10-1082/-819/-592)的等位基因型和TNFα-308、LTα(淋巴毒素α,亦称TNFβ)Nco Ⅰ、AspH Ⅰ双等位基因型分布;用ELISA法检测血清中Hp-IgG及cagA-IgG抗体水平.结果 (1)IL-10-1082 AG+GG基因型在胃癌组、慢性胃炎组及胃十二指肠溃疡组(非胃癌组)、健康对照组分布频率分别为20.0%、7.5%、6.0%和5.0%,IL-10-1082 AG+GG基因型分布在非胃痛组及健康对照组之间差异无统计学意义(P>0.05),而胃癌组高于非胃癌组(P<0.05)及健康对照组(P<0.05),其差异均有统计学意义.胃癌组中IL-10-592及-819两个位点与非胃癌组及健康对照组比较,基因型分布差异无统计学意义(P>0.05).IL-10-819位点与IL-10-592位点基因型分布频率一致.(2)胃癌组与其余3组比较,IL-10-1082 AG+GG基因型且Hp阳性的分布频率的差异有统计学意义(P<0.05).(3)LTα Nco Ⅰ AG基因型在Hp阳性胃癌患者中(66.7%)高于Hp阳性的健康对照组(47.8%),差异有统计学意义(P<0.05),该基因型与其他胃十二指肠疾病尤相关性.TNFα-308、LTα AspH Ⅰ与Hp感染及胃十二指肠疾病亦无相关性.结论 (1)IL-10-1082 AG+GG基因型与湖北地区汉族人群胃癌发生有相关性.(2)IL-10-1082 AG+GG基因型和LTα Nco Ⅰ AG基因型与湖北汉族人群Hp阳性胃癌有相关性.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.     

白细胞介素-10和肿瘤坏死因子基因多态性与湖北汉族人群胃十二指肠疾病及幽门螺杆菌感染的相关性

Objective To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection. Methods Six hundred and five patients with gastroduedenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFα-308, lymphotoxin-α (LTα) Nco Ⅰ and AspH Ⅰ gene polymorphisms. Hp infection status was determined with ELLS& Results (1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P <0. 05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients,non-malignant gastric disease patients and healthy controls (P>0. 05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0. 05). (3) Frequency of LTα Nco I AG genotype in gastric cancer patients with Hp infection was signiilcandy higher than that in Hp positive healthy controls (P < 0. 05). There were no other associations between TNFα-308, LTα Nco Ⅰ and AspH Ⅰ gene polymorphisms and Hp infection in gastroduodenal diseases. Conclusions (1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG,LTct Nco ⅠAG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.