共查询到19条相似文献,搜索用时 250 毫秒
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本研究结果发现,黄芪对柯萨奇B3病毒(CVB3)感染大鼠心肌细胞及正常对照心肌细胞的Ca^2+内流均呈显著抑制作用(P<0.01,P<0.05);若在病毒感染1h后加黄芪,经48h培养,对正常对照及感染细胞的Ca^2+内流亦有抑制作用(P<0.05)。同时细胞中CVB3-RNA含量显著少于病毒对照组(P<0.001)。提示黄芪具有钙拮抗作用,可减少病毒感染引起的心肌Ca^2+内流量,有可能减轻感染 相似文献
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目的 探讨颅脑损伤后神经细胞钙超载及钙-钙调蛋白复合物形成与三七总皂甙的治疗作用。方法 应用放射免疫法,原子吸收分光光度法测定动物血及脑组织中钙(Ca^2+),钙调蛋白(CaM)含量,观察三七总皂甙对动物脑损伤后上述指标改变的影响,结果 知中Ca^2+,CaM与及职Ca^2+,CaM呈显著正相关,三七总皂甙显著降低低血与脑组织中Ca^2+,CaM的含量,结论:三七总皂甙能阻滞脑损伤后神经细胞内钙超 相似文献
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运用SD大鼠自然更年期模型,观察大鼠心肌线粒体Ca^2+,Mg^2+-ATP酶,Ca^2+-ATP酶的变化,结果模型组大鼠Ca^2+,Mg^2+-ATP酶、Ca^+-ATP酶活性较组明显抑制;而应用理更年平调冲剂能提高更年期大鼠心肌线粒体,ATP酶活性,说明更年平调冲剂对于保护心肌线粒体,影响能量代谢,防治更年期心血管疾患具有良好作用。 相似文献
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首乌复方对兔红细胞膜Ca2+,Mg2+-ATPase活性的影响 总被引:6,自引:0,他引:6
研究首乌复方对高血脂兔红细胞膜Ca^2+,Mg^2+-ATPase活性的影响,实验结果表明,高脂饲料能显著地引起兔血浆中总胆固醇积累;也使兔血红细胞膜Ca^2+,Mg^2+-ATPase的基础活性降低近20%;而依赖钙调素(CaM)激活的Ca^2+,Mg^2+-ATPase的活性则是正常兔的144%。首乌复方不但能有效地阻止血液中胆固醇的积累,而且还能使Ca^2+,Mg^2+-ATPase的基础活 相似文献
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目的;观察雷公藤红素对血管平滑肌细胞内游离Ca^2+和H^+浓度的影响。方法:采用荧光染色法,参激光扫描焦显微镜动态研究了不同浓度的胎牛血清对单个血管平滑肌内游离Ca^2+和H^+浓度变化的影响效应。结果:30%胎牛血清可以引起细胞内一快速的,持续的游离Ca^2+浓度升高,而30%胎牛血清加0.3mg/L雷公藤红素则可以产生抑制细胞内游离Ca^2+浓度升高的效应; 相似文献
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电针对因性低血压家兔心肌细胞游离钙镁浓度的影响 总被引:1,自引:0,他引:1
用AR-CM-COM阳离子测定系统和离子探针Fura-2-Am及Furaptra-Am观察了电针在治疗家兔实验性低血压时心肌细胞内游离钙离子浓度(「Ca^2+」i)和游离镁离子浓度(「Mg^2+」i)的变化情况。股动脉放血造成家兔失血性低血压,心肌细胞「Ca^2+」i随着血压降低而降低,「Mg^2+」i则升高,「Ca62+」i/「Mg^2+」i比值上;电针拟“人中”、“承浆”穴位后,治疗组家兔血压 相似文献
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为探求清开灵注射液治疗中风的机制。以家兔自体血凝块复制脑出血模型,用干湿比重法及原子吸收分光光度计分别检测脑组织水份与Na^+、K^+、Ca^+、Mg^2+含量并且伊文斯蓝观察血脑屏障通透性变化。脑血肿形成后,脑组织水,Na^+、Ca^2+,EB含量明显增加,,K^+,Mg^2+降低,并有过度通气的血气值变化,EB含量的升高,反映出BBB受损,通透性增高。清开灵注射液能显著改善血气异常,降低BBB 相似文献
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目的探讨玉竹乙醇提取物和分离部位对糖尿病大鼠肾脏的保护作用。方法单次ip链脲佐菌素(STZ)60mg/kg建立实验性糖尿病大鼠模型,分别ig给予玉竹乙醇提取物和三氯甲烷、正丁醇分离部位。给药80d后测定血清肌酐(Cr)、尿素(Ur)、糖化血红蛋白(GHb)、肾皮质蛋白质非酶糖基化终末产物(AGEs)、尿白蛋白(UAL)排泄率,并观察肾组织病理学改变。结果玉竹乙醇提取物和三氯甲烷分离部位降低糖尿病大鼠血中GHb水平和UAL排泄率,抑制肾皮质AGEs的形成,同时改善肾脏病理改变;正丁醇分离部位降低UAL排泄率。结论玉竹乙醇提取物和三氯甲烷分离部位对糖尿病大鼠肾脏保护作用机制可能与抑制AGEs形成有关。 相似文献
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目的:探讨中药生脉注射液对心肌细胞内钙调节功能的影响。方法:采用 Fu-ra-2/AM 荧光标记及同位素~(45)Ca~(2 )负载示踪法测定了心肌细胞内钙浓度及肌浆网摄钙功能。结果:生脉注射液作用早期(10min)能够增加心肌细胞外钙的快相内流,使心肌细胞内游离钙浓度增高,肌浆网摄钙量也有明显增加;生脉注射液作用60min 时,与对照组比较细胞内~(45)Ca~(2 )总量增加,肌浆网对~(45)Ca~(2 )的摄取量明显增高,而细胞浆内游离钙浓度无明显变化。维拉帕米可阻断生脉注射液对细胞内钙的调节作用。结论:生脉注射液通过增加心肌细胞钙内流速度和肌浆网摄钙量,增强了心肌细胞钙贮备能力,进而调节心肌细胞的收缩舒张功能。 相似文献
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Effect of Brazilian green propolis on the production of reactive oxygen species by stimulated neutrophils 总被引:3,自引:0,他引:3
Simões LM Gregório LE Da Silva Filho AA de Souza ML Azzolini AE Bastos JK Lucisano-Valim YM 《Journal of ethnopharmacology》2004,94(1):59-65
The activity of a crude ethanol extract of green propolis and its fractions obtained by partition with hexane, chloroform and n-butanol was assessed on luminol- and lucigenin- enhanced chemiluminescence (CL) produced by rabbit neutrophils (PMNs) stimulated with particles of serum-opsonized zymosan (OZ). The total production of reactive oxygen species (ROS) by PMNs was measured by the luminol-enhanced CL (LumCL) assay and the production of the superoxide anion (O2*-) by the lucigenin-enhanced CL (LucCL) assay. All evaluated propolis samples had inhibitory effect on the LumCL and LucCL, which was concentration dependent. The n-butanol and chloroform fractions displayed the highest inhibitory effect on the LumCL produced by PMNs stimulated with OZ, in comparison with both the ethanol extract and the hexane fraction. Besides, the hexane fraction was the one which presented the highest effect for the LucCL assay. Some isolated compounds from both n-butanol and chloroform fractions were also assessed, including kaempferide, isosakuranetin, aromadendrine-4'-methyl-ether and 3-prenyl-p-coumaric acid. Kaempferide presented the highest inhibitory effect on the LumCL in comparison with the other compounds. Moreover, under the conditions assessed, the studied green propolis samples and isolated compounds were not toxic to the rabbit PMNs. 相似文献
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The antiinflammatory, analgesic and antimicrobial activities of crude ethanol extracts of Sideritis lotsyi var. mascaensis (Lamiaceae), and chloroform and aqueous fractions were evaluated in mice using paw and ear oedema induced by carrageenan and 12-o-tetradecanoyl-phorbol-acetate (TPA), respectively, as inflammation models, the writhing test induced by acetic acid for evaluating analgesic activity and the disk-diffusion method for testing antimicrobial actions. The results obtained demonstrated significant topical antiinflammatory and analgesic activities for the ethanol extract and chloroform fraction, but no relevant antimicrobial activity against the microorganisms tested. 相似文献
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泥胡菜黄酮类化学成分研究 总被引:1,自引:1,他引:0
目的:研究泥湖菜乙醇提取物氯仿和乙酸乙酯分离部位化学成分。方法:采用硅胶柱色谱,ODS柱色谱,Sephadex LH-20柱色谱和制备液相色谱等手段进行化学成分分离,运用理化性质和波谱数据(MS,1 H-NMR,13 C-NMR,HMBC等)鉴定化合物结构。结果:从泥湖菜乙醇提取物氯仿和乙酸乙酯分离部位分离得到8个黄酮类化合物,分别鉴定为金合欢素(1),玄参黄酮(2),芹菜素(3),山萘酚(4),山萘酚-3-O-β-D-吡喃葡萄糖苷(5),山萘酚-7-O-β-D-吡喃葡萄糖苷(6),山萘酚-3-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖苷(7),金合欢素-7-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖苷(8)。结论:化合物5为首次从该属植物中分离得到。 相似文献
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目的观察海康灵对SH-SY5Y神经细胞凋亡、细胞内线粒体膜电位和钙离子的影响。方法采用血清药理学方法,用流式细胞仪观察海康灵对过氧化氢(H2O2)损伤的SH-SY5Y神经细胞凋亡、细胞线粒体膜电位和钙离子的影响。结果与模型组比较,海康灵含药血清能明显抑制H2O2引起的SH-SY5Y神经细胞的凋亡,稳定线粒体膜电位,抑制钙离子内流。结论以上结果提示,海康灵可通过抑制胞浆钙离子升高,进而稳定线粒体膜电位,从而发挥其抑制神经细胞凋亡的作用。 相似文献
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Davilla rugosa Poiret ("Cipó- Caboclo") is commonly used in Brazilian folk medicine. The hydroalcoholic extract of the stems (HE) was fractionated with chloroform (CF), chloroform/ethyl acetate (CAF), ethyl acetate (AF), ethyl acetate/ethanol (AEF), ethanol (EF), and ethanol/water (EWF). The hydroalcoholic extract (HE) and fractions of the stems of D. rugosa Poiret were investigated for possible anti gastric ulcer properties. These extracts were shown to protect rats from developing gastric ulcers induced by two acute models: HCl/ethanol (400 mg/kg i.p.) and immersion-restraint stress (15 and 30 mg/kg of the HE and 15 mg/kg either of the ethanol or the ethanol/water fractions, p.o.). The daily oral dose of 800 mg/kg of HE for 30 consecutive days was tested for possible toxic effects. There were no modifications in body weight, water or food intake or in the external aspect of kidneys, spleen, lungs and liver. The only difference observed was a decrease of liver weight. These results suggest that the D. rugosa Poiret HE has an antiulcer activity in rats and the active components are in the two more polar fractions. 相似文献
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甜菜碱对HepG2人肝癌细胞[Ca2+]i和细胞膜电位的影响 总被引:1,自引:0,他引:1
目的研究甜菜碱对肝癌HepG2细胞内游离Ca2 浓度([Ca2 ]i)和细胞膜电位的影响。方法Fluo-3/AM标记,激光扫描共聚焦显微镜观察HepG2细胞[Ca2 ]i,TMRE标记,激光扫描共聚焦显微镜测定细胞膜电位的影响。结果甜菜碱能够升高HepG2细胞[Ca2 ]i,降低细胞膜电位。升高[Ca2 ]i的强度具一定的剂量依赖性,随甜菜碱剂量的增大而升高。甜菜碱50、25mg/mL组与对照组比较差异非常显著(P<0.01),甜菜碱12.5mg/mL组与对照组比较差异显著(P<0.05)。给药48h时间段内细胞内的[Ca2 ]i升高的幅度较大,72h升高幅度相对较小。而降低膜电位上,在48h时间段内,甜菜碱50、25、12.5mg/mL各组膜电位均显著高于对照组(P<0.05),但在72h时间段内甜菜碱12.5mg/mL组与对照组比较没有显著性差异(P>0.05)。结论甜菜碱通过开放细胞膜上的通透性转换孔道(MPTP),从而升高HepG2细胞[Ca2 ]i,启动细胞凋亡机制,诱导HepG2细胞凋亡。 相似文献
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目的:比较省沽油属4种植物干燥叶片中不同提取部位总酚类物质含量及其抗氧化活性测定,为开发利用打下基础。方法:用70%乙醇提取各植物干燥叶片,浓缩得乙醇浸膏,并用系统溶剂萃取法得到乙酸乙酯和氯仿两个部位;然后采用改良的Folin-Ciocalteu比色法测定各植物各部位总酚类物质含量,并通过DPPH氧化自由基清除活性实验及抗过氧化亚硝酸盐硝化活性实验分析各总酚类物质抗氧化活性。结果:比色法结果表明,四种植物中乙酸乙酯部位总酚类物质含量均高于氯仿部位,其中省沽油干燥叶片乙酸乙酯部位总酚类物质含量最高,抗氧化活性最强。结论:省沽油属植物叶片中含有活性酚类物质,其中省沽油叶片乙酸乙酯部位含量最高,活性最强,值得进一步开发利用。 相似文献