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Masatoshi SEKI 《Pathology international》1970,20(2):141-152
For the purpose to study the humoral control of defensive responses, kinetics of colony forming cells (GFC) In peripheral blood of histamine -injected mice was assayed by modification of McCulloch and Till's method, with usual hematological examinations. Effects of histamine on adrenalectomized mice and hydrocortisone injection into intact animals were also examined in the same manner. In conventional mice, histamine caused marked leukopenia and a rise of hematocrit value by 15 minutes after the injection, the latter of which was restored within 30 minutes. On the other hand, high hematocrit value continued for more than 1 hour in adrenalectomized mice.
Histamine administration into conventional mice caused a temporary increase of peripheral CFC by 1–3 hours after the injection, accompanied by granulocytosis. Afterwards, CFG count reverted to control level. In adrenalectomized mice, count of peripheral CFG was about two times of that of control. Histamine administration into adrenalectomized mice caused remarkable increase of peripheral CFC by 3 hours after the injection. On the contrary, hydrocortisone decreased peripheral CFC count distinctly. ACTA PATH. JAP. 20: 141 - 152, 1970. 相似文献
Histamine administration into conventional mice caused a temporary increase of peripheral CFC by 1–3 hours after the injection, accompanied by granulocytosis. Afterwards, CFG count reverted to control level. In adrenalectomized mice, count of peripheral CFG was about two times of that of control. Histamine administration into adrenalectomized mice caused remarkable increase of peripheral CFC by 3 hours after the injection. On the contrary, hydrocortisone decreased peripheral CFC count distinctly. ACTA PATH. JAP. 20: 141 - 152, 1970. 相似文献
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人脐血非造血干细胞免疫原性的实验研究 总被引:1,自引:0,他引:1
目的 探讨人脐血非造血干细胞免疫原性,为脐血非造血干细胞的应用提供依据.方法 通过流式细胞术检测人脐血非造血干细胞的免疫表型;体外混合淋巴细胞培养观察人脐血非造血干细胞原代、P1代、诱导分化细胞及干扰素-γ(IFN-γ)刺激异种T淋巴细胞增殖活化作用.结果 人脐血非造血于细胞表达HLA-ABC,微弱表达HLA-DR,经IFN-γ处理后,未明显改变HLA-ABC、HLA-DR的表达水平.混合淋巴细胞培养未见各处理组人脐血非造血干细胞明显刺激T淋巴细胞的增殖.结论 人脐血非造血干细胞无论原代、传代细胞、分化细胞及IFN-γ处理的细胞免疫原性均较弱. 相似文献
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Heparin is capable of enhancing the rate of release of antigen from nephritic rat kidneys. It also interferes with the binding of immune complexes by cultured glomerular mesangial cells. Postulating that these two effects might be related, we sought to determine what basic aspects of the molecular structure of heparin are responsible for the interference with binding in vitro . After cultured mesangial cells had bound radiolabelled synthetic immune complexes, heparin or a variety of structurally related molecules were added to the supernatant. De-N-sulphated heparin, heparan sulphate, low molecular weight heparin, and low molecular weight dextran sulphate had no effect on immune complex binding. High molecular weight dextran sulphate was able, like heparin, to dislodge immune complexes from mesangial cells, suggesting that high molecular weight and high sulphation are required. These results differ from previous findings in vivo , suggesting that the effect of heparin in vivo is not due to interaction at the mesangial cell surface. Alternative explanations for the effect of heparin in the intact animal include destabilization of the immune complex structure or, more probably, an effect at the boundary between the immune complex deposit and the basement membrane. 相似文献
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直接PCR法检测血液中伊氏锥虫感染 总被引:2,自引:0,他引:2
为建立伊氏锥虫动基体DNA(KinetoplastDNA,kDNA)的PCR扩增技术,血液直接PCR法检测伊氏锥虫的感染,本文以伊氏锥虫kDNA片段为靶扩增DNA设计引物,确定最适PCR反应条件,建立kDNA片段的PCR扩增技术,应用直接扩增缓冲液(Ampdirect)从感染小鼠的滤纸血标本直接PCR检测伊氏锥虫。结果发现,PCR扩增伊氏锥虫kDNA片段分子量为364bp,能检测的最小DNA量是0·06pg,与布氏锥虫、活动锥虫没有交叉反应。应用直接扩增缓冲液不需进行样本的DNA抽提,直接PCR能检测感染小鼠的早期滤纸血样本,敏感性高于镜检法。本实验建立的直接PCR法检测伊氏锥虫具有较高的敏感性和特异性,方法快捷,可进一步应用于伊氏锥虫病的早期诊断以及现场调查。 相似文献