三维血管瘤血管生成体外模型的建立

目的 建屯三维血管瘤血管生成体外培养模型.方法 将10例新鲜的增殖期血管瘤组织块置于纤维蛋白凝胶中以双层夹心法建屯三维血管瘤血管生成体外培养模型.于血管新生后3 d加入平阳霉素(PYM.1×102mg/L)做干预实验.镜下观察新生血管情况,SP免疫组织化学法检测第Ⅷ因子相关抗原(FⅧR-Ag)、透射电镜观察内皮细胞超微结构鉴定新生血管.结果 血管瘤组织培养4～7 d后芽生出细小m管,至第8～10大长成枝丫状血管样结构,第11～14天之后血管样结构生长渐缓停滞萎缩.PYM作用1一2 d后血管样结构很快萎缩.组织周边新生树枝状结构经鉴定为血管内皮细胞.结论 该模型部分程度上可代表血管生成、萎缩的过程,但细小血管样结构的芽生时间不等,萎缩较快,与体内血管瘤血管仍有一定区别.     

局部及转移骨肉瘤中VEGF、p53表达及微血管生成的对照研究

目的探讨血管内皮生长因子(VEGF)和p53在骨肉瘤微血管新生中的作用及对骨肉瘤侵袭性转移性的影响.方法采用免疫组织化学方法,检测57例局部原发性骨肉瘤和27例转移骨肉瘤原发灶标本中VEGF、p53的表达,计算MVD值.对比两骨肉瘤组中VEGF、p53表达、MVD值以及其它临床病理因子的差别.结果转移骨肉瘤组VEGF阳性表达率和MVD高于局部骨肉瘤组(P=0.049 4,P=0.030 2);p53蛋白表达在两组肿瘤中无显著差别(P＞0.05);转移骨肉瘤组中肿瘤平均直径大于局部骨肉瘤组的平均直径(P=0.035 0;VEGF和p53表达阳性组与阴性组相比,MVD值高(P=0.012 5,P=0.003 6);VEGF与p53表达阴阳性一致率为51.2%.VEGF和p53共同阳性表达组MVD值最高(42.7±22.4),而VEGF和p53共同阴性表达组MVD值为最低(23.3±20.1),差别显著(P=0.007 7).结论p53和VEGF共同参与骨肉瘤血管生成并有协同作用.多血管质骨肉瘤侵袭性强并易发早期转移.     

胃癌中血小板衍生内皮细胞生长因子的表达与微血管生成

目的研究探讨胃癌组织中血小板衍生内皮细胞生长因子（PD-ECGF）的表达及微血管密度（MVD）与胃癌生物学行为和血管生成的关系。方法应用免疫组化Elivision^TM法检测52份胃癌组织中PD—ECGF、CD34的表达并测定MVD，回顾分析胃癌患者的临床病理资料。结果胃癌组织中PD—ECGF的表达率为59．62％，平均MVD为72．60±16．87；伴有淋巴结转移组、侵及浆膜或浆膜外组的PD—ECGF的阳性率及MVD显著高于无淋巴结转移组、浸润深度未达浆膜层组，且Ⅲ期和Ⅳ期组的MVD高于Ⅰ期和Ⅱ期组；PD—ECGF阳性组的MVD高于阴性组。结论PD—ECGF参与了胃癌的浸润和转移及血管生成，PD—ECGF作为肿瘤抗血管治疗的靶点具有一定的价值。     

胆囊癌中促血管生成因子的表达与微血管计数的关系

本研究旨在探讨胆囊癌组织中两种最重要的促血管生成因子———血管内皮生长因子 (VEGF)、碱性成纤维细胞生长因子 (bFGF)的表达及其与微血管计数(MVC)的关系和意义。一、材料与方法1.标本及临床病理资料 :收集中国医科大学附属第一、第二医院 1999年 3月～ 2 0 0 1年 12月间胆囊癌手术切除标本存档蜡块 49例。其中男 2 4例 ,女 2 5例 ;男性年龄 (62 .0± 9.8)岁 ,女性年龄(5 4.5± 9.3 )岁。病理组织学类型均为腺癌 ,其中乳头状腺癌 6例 ,占 12 .2 % ,其余 43例腺癌的分化程度为 :高分化 16例 (3 2 .7% ) ,中分化 17例 (3 4.7% ) ,低…     

一种三维血管瘤血管生成体外培养模型的建立

目的 建立一种三维血管瘤血管生成体外培养模型.方法 将新鲜的增殖期血管瘤手术标本置于纤维蛋白凝胶,以双层夹心法建立三维血管瘤血管生成体外培养模型.结果 血管瘤组织培养2～3 d后芽生出细小血管,后呈枝丫状生长,至第8～9天长成树枝状血管,分叉交叠,之后血管生长渐缓停滞.组织周边新生树枝状结构经石蜡切片HE染色、血管内皮细胞特异性标志物第Ⅷ因子相关抗原检测及电镜观察,鉴定为血管.结论 该模型的成功建立有助于血管瘤血管生成机制及抗血管生成药物的研究.     

角质细胞生长因子和环氧化酶-2在胃癌中的表达及其与血管生成的关系

目的检测角质细胞生长因子(KGF)和环氧化酶-2(COX-2)蛋白在胃癌组织中的表达及微血管密度(MVD),探讨KGF与COX-2在胃癌发生、发展中的作用及其机理。方法采用免疫组化SP法检测64例胃癌组织及30例正常胃黏膜组织中KGF和COX-2蛋白表达,并采用CD34抗体染色检测MVD。结果 KGF和COX-2蛋白在胃癌组织中的表达阳性率分别为65.6%(42/64)和79.7%(51/64),分别高于其在正常胃黏膜组织中的表达阳性率〔23.3%(7/30)和13.3%(4/30)〕,P=0.046、P=0.008。胃癌组织MVD为31.8±8.0,明显高于正常胃黏膜组织的14.3±6.1(P=0.000);KGF与COX-2蛋白均表达阳性者MVD为35.9±5.7,明显高于两者表达均为阴性者的25.7±7.0(P=0.000)。胃癌组织中KGF和COX-2蛋白表达均与淋巴结转移、浆膜浸润及TNM分期有关(P<0.05、P<0.01),MVD与淋巴结转移和TNM分期有关(P<0.01),但均与患者年龄、性别以及肿瘤分化程度无关(P>0.05)。KGF与COX-2联合表达者与胃癌的浆膜浸润、淋巴结转移和TNM分期有关(P<0.05),与患者年龄、性别以及肿瘤分化程度无关(P>0.05)。胃癌组织中KGF与COX-2蛋白表达呈正相关(r=0.610,P=0.000);胃癌组织MVD与KGF和COX-2蛋白表达均呈正相关(r=0.675,P=0.000;r=0.657,P=0.000)。结论 KGF和COX-2蛋白在胃癌组织中高表达,可能通过促进肿瘤新生血管的生成协同参与胃癌的浸润、转移。     

血管生成与动脉生成

出生后的血管再生方式主要包括血管生成和动脉生成。血管生成与动脉生成过程中可以发现两者在发生的部位、诱发和调节因素、结果方面有着一定的区别和联系。大部分的缺血性血管疾病需要同时促进血管生成和动脉生成。因此 ,联合应用生长因子、炎症因子和细胞来促进血管生成与动脉生成 ,恢复缺血区的血液供应、改善组织细胞的氧和营养物质供给 ,才能全面的治疗缺血性血管疾病 ,它将成为缺血性血管疾病治疗的发展方向     

血管生成与胆囊癌发展和预后的关系

目的　探讨肿瘤微血管密度(MVD)与胆囊癌的侵袭转移和预后的关系。方法　应用免疫组化法,用抗人因子Ⅷ相关抗原(FⅧRAg)的单克隆抗体测定42例人胆囊癌组织中的MVD,并分析其与胆囊癌各种病理因素及预后之间的关系。结果　MVD与胆囊癌浸润深度(P＜0.05)、淋巴结转移(P＜0.01)和远处转移(P＜0.05)密切相关,而与病理类型、分化程度无关(P＞0.05);MVD＞54.82的胆囊癌患者5年生存率较低。结论　肿瘤微血管密度与胆囊癌浸润深度、淋巴结及远处转移密切相关,对判断胆囊癌的预后具有重要的临床意义。     

异种微血管移植的实验研究

为了寻找一种新的微血管代用品，采用冷冻干燥人胎盘血管作为异体移植物，修复兔股动脉缺损为实验组；未经处理的胎盘血管作为对照组，观察其通畅率及组织学改变。结果表明，实验组术后２周通畅率为８５％，但术后３个月其通畅率则下降到５５％。从组织学和电子微镜扫描观察，显示最初的移植血管分解，并且在通畅血管上被一个新的纤维层所代替。认为用胎盘血管作为移植材料修复血管缺损尚需进一步研究。     

Training model for microvessel anastomosis

In the early stages of microsurgical training a model is required which is accessible, inexpensive, and approximates human tissue. In this research centre initial training exercises utilize cold stored vessels harvested from sacrificed animals used in other experiments. This model serves as an initial training tool prior to the progression to live animal research which maximizes use of animal specimens and approximates the in vivo qualities of blood vessels. The costs involved are minimal and a large number of vessels can be efficiently harvested and stored using basic equipment. As a training model, cold stored vessels have balanced cost with practicality, given an excellent approximation to living tissue and have markedly reduced our usage of live animals for teaching. This latter effect is significant at a time of increased scrutiny of the ethical use of laboratory animals. © 1994 Wiley-Liss, Inc.     

External aortic annuloplasty ring for valve-sparing procedures

Two different surgical approaches have been suggested for aortic valve-sparing surgery. My colleagues and I suggest combining the advantages of both approaches by adding an external subvalvular prosthetic ring annuloplasty to the remodeling procedure.     

In vitro non-rigid life-size model of aortic arch aneurysm for endovascular prosthesis assessment.

PURPOSE: It is essential to evaluate new stent designs before in vivo testing. The purpose of this study was to develop and validate a controlled and reproducible patient-derived process to produce a life-size in vitro model of aortic arch aneurysm for endovascular procedure simulation. METHODS: A three-dimensional magnetic resonance angiography (3D MRA) image derived from a 60-year-old patient with aortic arch aneurysm was segmented using a home-made software package which allows one-click automatic segmentation of the aorta, meshing, and conversion to standard tessellation language (STL) format. A rapid prototyping technique established a stereolithographic model to produce a replica of the whole aorta, including the arch aneurysm and supra-aortic arteries. RESULTS: The final model was made by pouring silicone rubber to obtain a sturdy, life-size, soft, transparent, plastic cast, accurately reproducing both the internal and external anatomy of the aortic aneurysm. This model was used under perfusion by an extracorporeal circulation pump, to test ex vivo stent deployment. CONCLUSION: The combination of easy segmentation and conversion to the STL format with industrial stereolithography techniques enabled a realistic silicon vascular phantom to be created for endovascular procedure simulation, image modality calibration, and new stent design.     

Development of an in vitro burn wound model

Healing of a deeper burn wound is a complex process that often leads to scar formation. Skin wound model systems are important for the development of treatments preventing scarring. The aim of this study is to develop a standardized in vitro burn wound model that resembles the in vivo situation. A burn wound (10 × 2 mm) was made in ex vivo skin and the skin samples were cultured at the air–liquid interface for 7, 14, and 21 days. Cells in the skin biopsies maintained their viability during the 21-day culture period. During culture, reepithelialization of the wound took place from the surrounding tissue and fibroblasts migrated into the wound area. Cells of the epithelial tongue and fibroblasts near the wound margin were proliferating. During culture, skin-derived antileukoproteinase and keratin 17 were expressed only in the epithelial tongue. Both collagen type IV and laminin were present underneath the newly formed epidermis, indicating that the basement membrane was restored. These results show that the burn wound model has many similarities to in vivo wound healing. This burn wound model may be useful to study different aspects of wound healing and testing pharmaceuticals and cosmetics on, e.g., migration and reepithelialization.