Fatty acid composition and arachidonic acid intake of selected Saudi foods

Twenty-eight Saudi Arabian common foods were analyzed for their fatty acid contents by gas-liquid chromatography using capillary column. The predominant fatty acids were oleic, palmitic, linoleic and stearic acids. The polyunsaturated to saturated fats ratio (P:S) as well as the n-3:n-6 ratio were generally not compatible with recommended values. Assessment of C20:4 (n-6) content in those foods showed a noticeable variation ranging from 9 mg/100 gm in beef sirloin to 256 mg/100 g in kannad fish. Eicosapentaenoic acid (C20:5) n-3 content was highest in kannad (925 mg/100 g). Applying C20:4 (n-6) values determined in this work to Saudi Balanced Sheet, the mean C20:4 (n-6) intake for a Saudian was estimated as approximately 115 mg/day.     

Fatty acid composition of Costa Rican foods including trans fatty acid content

To further understand the fatty acid–disease relationships in an epidemiologic context, detailed composition tables are required. The composition of the major saturated, monounsaturated, and polyunsaturated fatty acids that are most abundant in the diet, is available for numerous foods in many countries, but data on the content of individual fatty acids with relative low abundance are scarce. We conducted a study to establish a fatty acid composition database that includes fatty acids with low relative abundance as well as trans fatty acids for the main sources of fat in the Costa Rican diet. Fatty acids were determined by gas chromatography. We present in-depth fatty acid composition tables for foods that are commonly used in Costa Rica. These analyses include information on alpha-linolenic, gamma-linolenic, arachidonic acid and trans fatty acids, which are important for health but where information is scarce. Of particular interest is the high content of trans fatty acids in partially hydrogenated soybean oil in Costa Rica, although decreasing over time. In a period of 10 years the amount of total trans fatty acids in Costa Rican soybean oil has decreased from an average of 20 to 1.5%, while alpha-linolenic has increased from an average of 1.87 to 6.06%. Our data will be of special interest for studies on Latin American countries requiring dietary information.     

Fatty acid composition including trans-fatty acids in selected cereal-based baked snacks from Lebanon

Ten samples of biscuits (including plain, whole meal, cream-filled and chocolate-coated), 7 samples of cakes (including plain, chocolate and cream-filled), 4 samples of wafers (including plain, chocolate-filled, coated and chocolate-filled) and 3 samples of croissants (including plain and chocolate-filled) were collected from Lebanon, in 2006, and analyzed for their fatty acid composition including trans fatty acids (TFA) by AgNO₃-thin layer chromatography and gas chromatography. All samples contained TFA ranging between 0.7 and 25.8 g/100 g fat and 20 out of the 24 analyzed samples contained more than 2% TFA on fat basis. The ratios of trans 18:1/(trans 18:2 + trans 18:3) ranged between 4 and 15.3 thereby indicating formulation of the products with partially hydrogenated oils. The content of TFA in the samples showed similarities to those reported in similar product categories in developing countries. This study is the first to report data on the levels and spectrum of TFA in snack products in Lebanon and underlines the importance of developing effective policies for reducing the intake of TFA by populations in the Middle East.     

Fatty acid composition of frequently consumed foods in Turkey with special emphasis on trans fatty acids

Fatty acid compositions of frequently consumed foods in Turkey were analyzed by capillary gas chromatography with particular emphasis on trans fatty acids. The survey was carried out on 134 samples that were categorized as meat products, chocolates, bakery products and others. The meat products except chicken-based foods have trans fatty acids, arising as a result of ruminant activity, with an average content of 1.45 g/100 g fatty acids. The conjugated linoleic acid content of meat and chicken doner kebabs were found higher than other meat products. Chocolate samples contained trans fatty acids less than 0.17 g/100 g fatty acids, with the exceptional national product of chocolate bars and hazelnut cocoa cream (2.03 and 3.68 g/100 g fatty acids, respectively). Bakery products have the highest trans fatty acid contents and ranged from 0.99 to 17.77 g/100 g fatty acids. The average trans fatty acid contents of infant formula and ice-cream, which are milk-based products, were 0.79 and 1.50 g/100 g fatty acids, respectively. Among the analyzed foods, it was found that coffee whitener and powdered whipped topping had the highest saturated fatty acid contents, with an average content of 98.71 g/100 g fatty acids.     

Fatty acid composition of frequently consumed foods in Turkey with special emphasis on trans fatty acids

Fatty acid compositions of frequently consumed foods in Turkey were analyzed by capillary gas chromatography with particular emphasis on trans fatty acids. The survey was carried out on 134 samples that were categorized as meat products, chocolates, bakery products and others. The meat products except chicken-based foods have trans fatty acids, arising as a result of ruminant activity, with an average content of 1.45 g/100 g fatty acids. The conjugated linoleic acid content of meat and chicken doner kebabs were found higher than other meat products. Chocolate samples contained trans fatty acids less than 0.17 g/100 g fatty acids, with the exceptional national product of chocolate bars and hazelnut cocoa cream (2.03 and 3.68 g/100 g fatty acids, respectively). Bakery products have the highest trans fatty acid contents and ranged from 0.99 to 17.77 g/100 g fatty acids. The average trans fatty acid contents of infant formula and ice-cream, which are milk-based products, were 0.79 and 1.50 g/100 g fatty acids, respectively. Among the analyzed foods, it was found that coffee whitener and powdered whipped topping had the highest saturated fatty acid contents, with an average content of 98.71 g/100 g fatty acids.     

Fatty acid and CLA composition of Brazilian dairy products, and contribution to daily intake of CLA

In the present study, the conjugated linoleic acid (CLA) contents of dairy products most consumed by the population living in the southeast region of Brazil (whole milk, “Prato” cheese and butter) were determined. In addition, the contribution of these dairy foods to the daily intake of CLA isomers was estimated. Fatty acid composition was determined by gas chromatography. Quantitative analysis of CLA isomers was done by silver-ion high-performance liquid chromatography (HPLC) and their identification was achieved by gas chromatography–mass spectrometry (GC–MS) of dimethyloxazoline (DMOX) derivatives. Eleven CLA isomers were identified and the predominant were cis-9,trans-11 and trans-7,cis-9. The total CLA contents in whole milk, “Prato” cheese and butter ranged from 4.0 to 8.4 mg/g fat and were similar to those reported for dairy products from other countries. Whole milk samples had significantly higher contents of total CLA, when compared to “Prato” cheese and butter, and the total CLA contents were significantly different between the commercial brands of each dairy product. The estimated average daily intake of total CLA in southeastern Brazil was 36 mg/day, which was lower than those of European and North American countries, and milk was the main food source of CLA in the diet.     

The nutritional composition of selected ethnic foods consumed in Italy

Assessment of the nutritional quality of some selected ethnic foods consumed in Italy was performed within the framework of the European Food Information Resource (EuroFIR 1 ) Network as part of the ethnic foods Work Package. A definition of ethnic and modified ethnic foods, as well as guidelines on the characterisation of immigrant populations and a detailed stepwise analysis of the ethnic food market, have been developed within the EuroFIR project. These were the basis for selecting ethnic foods in Italy for nutritional analysis. Four main immigrant groups (Asian, Latin American, African and Eastern European) were identified as commonly selected from those residing in Italy, and 25 dishes or composite foods consumed by these ethnic groups were selected. Based on consumption rates and market share of these foods in Italy, five ethnic food types (cantonese rice, nachos, falafel, kebab, sarmale) were chosen and collected for analysis. Nutritional evaluation of the selected foods was performed by analysing the following components: water, nitrogen, fat, total sugars, starch, total ash and dietary fibre, fatty acid composition, cholesterol, minerals (Na, Ca, Fe, P, Mg, K, Cu, S, Zn and Cl), and vitamins (vitamins A, E, B1, B2, B6, C, biotin, niacin and β‐carotene). Procedures for collection of the chosen foods, along with the results of the analysis, are described in the present paper. These new data will be added to the Italian Food Composition Tables.     

青岛居民膳食脂肪摄人量及脂肪酸构成的调查分析

目的探析青岛居民膳食中脂肪摄入量及脂肪酸构成情况,以及其与血脂异常的相关性。为引导居民合理膳食,预防心血管病（CHD）的发生提供科学依据。方法2010年3月至7月期间采用营养流行病学调查方法对健康体检人群进行整群随机抽样选取189例研究对象（男性53例,女性136例）。分别进行体格检查、空腹血三酰甘油（TG）、胆固醇（TC）、低密度脂蛋白胆固醇（LDL—C）、高密度脂蛋白胆固醇（HDL—C）、血糖（Glu）检查膳食调查、膳食脂肪酸分析以及膳食脂肪与血脂相关性的分析。结果青岛居民日人均摄入量为粮谷类200g,薯类26g,畜禽肉及内脏类85g,海产品73g,坚果类23g,油脂类40g。蛋白质占摄入总热量16％,碳水化合物48％,脂肪37％。油脂类占脂肪摄入43％,坚果类占11％。饱和脂肪酸（S）：单不饱和脂肪酸（M）：多不饱和脂肪酸（P）（S：M：P=1：1．5：1．49,n-6／n-3=23）。脂肪摄入量较高,其主要原因是植物油及坚果类摄人量较多。ω-3多不饱和脂肪酸（n-3PUFAs）食物摄入量较低,长链n一3PUFAs为0．15g。血脂异常人群营养素摄人量和体质指数（BMI）均高于健康人群;脂肪日人均摄入量越高TC、TG、LDL水平越高,而HDL水平下降,长链n-3PUFAs对TG、LDL有正向调节的作用。结论青岛居民的膳食脂肪摄入量较高,长链n-3PUFAS的摄入量偏低。长链n-3PUFAS与血脂异常有负相关性,减少该地区居民膳食总脂肪摄入,增加膳食中富含n-3PUFAs的海鱼产品摄入,对该地区居民控制血脂,防治CHD的发生具有重要意义。     

Fatty acid composition of prepared infant formulas

The fatty acid composition of eight American, eight European, and four Japanese prepared infant formulas were determined and compared with fat sources listed on labels. Unsaturated fatty acids ranged from 20 to 83 per cent of the total. Generally, the fatty acid composition was consistent with the types of ingredients used; those with corn or soy oil had the highest levels of unsaturated fat, and those with milk fat the lowest; formulas of mixed composition fell in between. The formulas were compared with values for the fatty acid composition of human milk in the literature.     

Fatty acid and amino acid compositions of selected wild-edible mushrooms consumed in Turkey

The fatty acid and amino acid compositions of 11 mushroom species commonly consumed were collected from the East Black Sea region of Turkey and analyzed. All species were characterized by a high content of linoleic acid (C18:2n-6) and glutamic acid. The highest content of linoleic acid (78.0%) and glutamic acid (29.4 μg/mg dry weight [d.w.]) was found in Agaricus arvensis and the lowest in Cantharellus tubaeformis, 19.8% and 10.9 μg/mg d.w., respectively. The average content of amino acids for all species was 148 μg/mg d.w. Overall, these results demonstrate that the 11 different kinds of wild edible mushrooms gathered from the region represent substantial sources of fatty acids and amino acids that are essential in the diet of humans. Quality of the mushroom protein compares favorably with the FAO/WHO Standard. The present study demonstrates that macrofungi from the East Black Sea region (Turkey) are a good source of many nutrients essential to human well-being.     

Fatty acid and amino acid compositions of selected wild-edible mushrooms consumed in Turkey

The fatty acid and amino acid compositions of 11 mushroom species commonly consumed were collected from the East Black Sea region of Turkey and analyzed. All species were characterized by a high content of linoleic acid (C18:2n-6) and glutamic acid. The highest content of linoleic acid (78.0%) and glutamic acid (29.4 μg/mg dry weight [d.w.]) was found in Agaricus arvensis and the lowest in Cantharellus tubaeformis, 19.8% and 10.9 μg/mg d.w., respectively. The average content of amino acids for all species was 148 μg/mg d.w. Overall, these results demonstrate that the 11 different kinds of wild edible mushrooms gathered from the region represent substantial sources of fatty acids and amino acids that are essential in the diet of humans. Quality of the mushroom protein compares favorably with the FAO/WHO Standard. The present study demonstrates that macrofungi from the East Black Sea region (Turkey) are a good source of many nutrients essential to human well-being.     

Fatty acid composition of adipose tissue and serum lipids are valid biological markers of dairy fat intake in men

The fatty acid intake is in part reflected by the fatty acid composition of adipose tissue and serum lipids. We evaluated whether the proportions of myristic (14:0), pentadecanoic (15:0) and heptadecanoic (17:0) fatty acids in the adipose tissue triacylglycerols and serum cholesterol esters and phospholipids reflect long-term dairy fat consumption in free-living men. In 114 healthy men aged 40-76 y, we compared the relative content of 14:0, 15:0 and 17:0 in subcutaneous adipose tissue and in serum lipids with relative intake (g/100 g of total fat) assessed by two 1-wk weighed food records made 6 mo apart and assessed by fourteen 24-h dietary recall interviews equally distributed during 1 y. According to food records, the mean +/- SD dairy fat intake was 24.9 +/- 13.1 g/d (29.6 +/- 10.5 g/100 g total fat); intake of 14:0, 15:0 and 17:0 was 4.6, 0.23 and 0.16 g/100 g total fat, and the content in adipose tissue was 3.6, 0.36 and 0.25 g/100 g fatty acids, respectively. Pearson correlation coefficients between intake of dairy fat (based on 24-h recalls) and fatty acid composition of adipose tissue were 0.64 (P: < 0.001) for 14:0, 0.74 (P: < 0.001) for 15:0 and 0.60 (P: < 0.001) for 15:0 + 17:0. Corresponding correlations with serum cholesterol esters were 0.34 (P: < 0.001) (14:0), 0.45 (P: < 0.001) (15:0) and 0.56 (P: < 0.001) (15:0 + 17:0), and with serum phospholipids the values were 0.30 (P: < 0.01) (14:0), 0.50 (P: < 0.001) (15:0) and 0.50 (P: < 0.001) (15:0 plus 17:0). In our study population, the relative content of 15:0 or 14:0 in adipose tissue is a valid biomarker for long-term dairy fat intake in free-living individuals. When adipose tissue is not available, 15:0 content in serum cholesterol esters or phospholipids might be used. Intake data based on repeated 24-h recalls are equally informative and may be an equivalent choice in nutritional studies.     

不同肉类食物中脂肪酸组成

目的研究5种肉类不同部位和内脏中的脂肪酸含量及其差异性分析。方法采集猪肉、牛肉、羊肉、鸡肉和鸭肉不同部位的样品,采用气相色谱测定样品中脂肪酸组成及含量。结果猪肉、反刍动物和禽肉类中的脂肪酸种类相似,但其构成比差异有统计学意义(P<0.05)。畜肉中含有较高的饱和脂肪酸,而其多不饱和脂肪酸含量较低,猪肉的饱和脂肪酸含量最低,多不饱和脂肪酸的含量高于牛肉和羊肉。反刍动物类含有较高的反式脂肪酸。禽肉中的饱和脂肪酸较低,多不饱和脂肪酸较高。结论肉类脂肪酸比例不平衡,可通过调控饲料中油脂组成及营养水平,从而改善畜禽肉质的营养价值。     

Plasma carotenoid response to chronic intake of selected foods and beta-carotene supplements in men.

We determined serial changes in four major plasma carotenoid fractions (alpha-carotene, beta-carotene, lutein/zeaxanthin, and lycopene) in 30 men consuming defined daily doses of carotenoids from foods (broccoli, carrots, or tomato juice) or from purified beta-carotene in capsules (12 or 30 mg) for 6 wk while fed a controlled diet. Compared with baseline, beta-carotene increased in the 30- and 12-mg-capsule and carrot groups whereas alpha-carotene increased in the carrot group and lutein increased in the broccoli group. Lower lutein concentrations in recipients of beta-carotene capsules suggested an interaction between these two carotenoids. Lycopene declined in all groups except the tomato-juice group. Total carotenoid concentration changes only reflected the large increases in beta-carotene concentrations and not the smaller changes observed in other individual carotenoids. Overall, purified beta-carotene produced a greater plasma response than did similar quantities of carotenoids from foods sources. However, some foods increased plasma concentrations of certain carotenoids.