Triterpene saponins from Lysimachia christinae

Seven triterpene saponins were isolated from Lysimachia christinae and identified as lysichriside A (1), lysichriside B (2), primulanin (3), lysikokianoside 1 (4), anagallisin C (5), ardisiacrispin A (6), and ardisicrenoside B (7). Compounds 1 and 2 are new triterpene saponins, compounds 3, 5, and 7 were isolated from this genus, and compounds 4 and 6 were isolated from this species for the first time. Their structures were elucidated by means of 1D and 2D NMR experiments.     

猫儿刺的化学成分

猫儿刺(Ilex pernyi)为冬青科冬青属植物，民间常用于清热解毒，润肺止咳。本文对猫儿刺叶的化学成分进行研究，应用硅胶、Sephadex LH-20、反相硅胶和半制备RP-HPLC等分离纯化方法从其乙醇提取物中分离并鉴定了6个化合物：(E)-异丁香酚-α-L-吡喃阿拉伯糖基(1→6)- β-D-吡喃葡糖苷(1)，山柰酚-3-O-桑布双糖苷(2)，槲皮素-3-O-桑布双糖苷(3)，异槲皮苷(4)，丁香树脂酚葡糖苷(5)，反枝苋苷IV(6)。其中，化合物1为一新酚苷类化合物，命名为猫儿刺酚苷A。化合物2～6为首次从该植物中分离得到。     

Two new constituents from mangrove Bruguiera gymnorrhiza

A lignan and two aromatic compounds were isolated from the branches of the mangrove plant, Bruguiera gymnorrhiza. They were brugunin A (1), bruguierol D (2) and 2,3-dimethoxy-5-propylphenol (3). Among them, 1 and 2 were new compounds; 3 was isolated from a natural source for the first time. The structures of these compounds were determined by NMR spectroscopic studies as well as chemical evidence.     

滨蒿的化学成分

目的进一步研究菊科植物滨蒿(Artemisia scoparia)中的化学成分。方法采用硅胶柱色谱法、中低压柱色谱法、ODS柱色谱法、Sephadex LH 20柱色谱法以及高效液色谱法进行分离和纯化,并依据理化性质和波谱解析鉴定其化学结构。结果从植物滨蒿地上部分的体积分数为60%乙醇提取物(420 g)中分得6个化合物,分别鉴定为3羟基二十二酸2(5乙酰基2,3二氢苯并呋喃2基)丙酯(1)、2′(3′甲氧基3甲基丁烯基)对甲氧基乙酰苯(2)、5,8二甲氧基6,7亚甲二氧基香豆素(3)、8甲氧基6,7亚甲二氧基香豆素(4)、6去甲氧基茵陈色原酮(5)、2,4二羟基6甲氧基乙酰苯(6)。结论化合物1~6均为首次从该植物中得到,其中化合物1、2为新化合物。     

海南粗榧中的苯丙素类化合物及其抗氧化活性

目的对海南粗榧的化学成分进行研究。方法采用多种色谱技术进行化合物的分离纯化，利用光谱技术和理化性质鉴定化合物的结构。采用DPPH法进行抗氧化活性测试。结果从海南粗榧的乙醇提取物中分离得到3个苯丙素类化合物，分别鉴定为junipediol A（1）、junipediol A 8-O-β-D-glucopyranoside（2）和junipediol B（3）。结论化合物1～3均为首次从该属植物中分离得到，3个化合物均具有抗氧化活性。     

人参芦头抗心律失常活性成分研究

从人参(Panax ginseng C.A.Meyer)芦头中分离得到10个化合物。经理化性质、波谱分析和薄层析分别鉴定为:20(R)-人参皂甙-Rh_1(Ⅰ),人参皂甙-Rg_3(Ⅱ),20(S)-人参皂甙-Rg_2(Ⅲ),-Rg_1(Ⅳ),-B(Ⅴ),-Rd(Ⅵ),-Rc(Ⅶ),-Rb_2(Ⅷ),-Rb_1(Ⅸ),-Ro(Ⅹ).其中化合物Ⅰ,Ⅱ为首次从人参芦头中分离得到的已知成分,并对其中9种主要人参皂甙进行了抗氯化钡诱发大白鼠心律失常作用的研究。     

地骨皮化学成分研究

目的研究地骨皮Lycium chinense的化学成分。方法采用硅胶柱色谱法结合制备高效液相进行分离和纯化,根据理化性质和波谱数据进行结构鉴定。结果分离得到8个化合物,即saikolignanoside A(1),东莨菪苷(scopolin,2),阿魏酸(ferulic acid,3),4-羟基苯甲酸(4-hydroxy-benzoic acid,4),3-羟基-1-(3-甲氧基-4-羟基-苯基)-丙基-1-酮(3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-1-one,5),反式-N-咖啡酰酪胺(trans-N-caffeoyltyramine,6),反式-N-阿魏酰酪胺(trans-N-feruloyltyramine,7),二氢咖啡酰酪胺(dihydro-N-caffeoyltyramine,8)。结论化合物1,5,7为首次从该属植物中分离得到。     

辽东楤木叶化学成分的分离与鉴定

目的对辽东楤木叶的化学成分进行研究。方法通过硅胶柱色谱、ODS柱色谱和制备HPLC等手段进行分离和纯化,利用理化性质和波谱数据进行结构鉴定。结果从辽东楤木叶中分离得到5个化合物,分别鉴定为glucocaulophyllogenin(1)、glycoside st-B(2)、silphioside E(3)、山萘酚-3-O-β-D-吡喃葡萄糖苷(4)、槲皮素(5)。结论化合物1为新天然产物,化合物2～4为首次从楤木属植物中分离得到,化合物5为首次从辽东楤木中分离得到。     

1株北极真菌Eutypella sp. D-1的生物活性成分研究

目的 对分离自北极高纬度地区的１株真菌Eutypella sp. D-1的抗肿瘤活性成分进行研究。方法 运用溶剂分部萃取、硅胶柱色谱、中压ODS柱色谱以及半制备HPLC等多种分离方法对次级代谢产物进行分离纯化;通过运用NMR、MS等波谱技术,结合文献比对,解析化合物的结构。结果 从真菌Eutypella sp. D-1大米培养基萃取物中分得3个化合物,鉴定为cladoacetal C (1)、benzophomopsin A (2)、pestalospirane B (3)。结论 化合物1为新化合物,化合物2~3均为首次从该属真菌中分离得到。     

真菌Neoascochyta argentina NBERC-H82的次级代谢产物研究

摘要：研究真菌Neoascochyta argentina中抗菌化学成分。采用硅胶、葡聚糖凝胶LH-20柱层析以及制备高效液相色谱等分离技术,从Neoascochyta argentina发酵液乙酸乙酯提取物中分离得到6个化合物,分别鉴定为ascomfuran A(1),phenylpyropene B(2),phenylpyropene C(3),phenylpyropene F(4),phenylpyropene A(5),pyripyropene A(6),所有化合物均首次从Neoascochyta属分离得到。采用微量稀释法,测试分离化合物对于5种病原细菌和4种病原真菌的抑制活性,结果表明化合物1,2,4和5对于灰葡萄孢菌具有抑制活性(MIC均为50 μg/mL)。研究结果表明真菌Neoascochyta argentina中主要代谢产物为pyripyropene和phenylpyropene类化合物,具有抑制灰葡萄孢菌的活性,为开发其成为微生物源农药提供理论依据。     

New cytotoxic triterpene acids from aboveground parts of Manihot esculenta from the Suriname rainforest

Two novel triterpene acids, esculentoic acid A ( 1) and B ( 2) as well as the seven known compounds 3 - 9 were isolated from an EtOAc extract of leaves, stems, and twigs of Manihot esculenta by bioassay-guided fractionation for cytotoxic activity. The structures of the two new compounds were established as 3alpha-hydroxytaraxer-14-en-29-oic acid ( 1) and 3-oxotaraxer-14-en-29-oic acid ( 2) on the basis of 1D and 2D NMR spectroscopic data interpretation and chemical conversions. The two new compounds 1 and 2 showed moderate cytotoxicity against the A2780 human ovarian cancer cell line.     

苦皮藤根皮的1HNMR指纹图解析

目的对苦皮藤的¹HNMR指纹图进行解析.方法用硅胶柱色谱法分离苦皮藤根皮CGEA的化学成分,鉴定单体化合物的结构并对其进行¹HNMR研究,从而实现苦皮藤根皮的¹HNMR指纹图解析.结果不同来源的苦皮藤样品,其¹HNMR指纹图有很好的重现性和高度的特征性.从其CGEA中分得3个主要成分,经光谱分析鉴定其结构,分别为:angulatinA(1),angulatinB(2)和angulatinC(3).结论苦皮藤根皮的¹HNMR指纹图可用于其基源鉴定,并主要显示了以上3个化合物的特征共振信号;化合物3为新化合物,化合物2为首次从苦皮藤分离得到.     

深海曲霉属真菌Aspergillus insulicola的化学成分研究

目的 对深海沉积物来源真菌Aspergillus insulicola中的化学成分进行研究。方法 采用正反向硅胶柱色谱、高效液相色谱等方法对其大米发酵产物进行分离提纯；通过质谱、核磁共振等波谱学方法并结合文献数据鉴定其结构。结果 从海洋真菌A. insulicola大米发酵产物的乙酸乙酯提取物中共分离鉴定了9个化合物,其结构分别为：aspermicrone C(1)、aspermicrone B(2)、epicolactone(3)、F14945-C(4)、6,7-dihydroxy-5-methoxy-4-methylisobenzofuran-1(3H)-one(5)、5,6-dihydroxy-7- methoxy-4-methylisobenzo- furan-1(3H)-one(6)、3-methoxyepicoccone(7)、3,4-dihydroxyphenylacetic acid methyl ester(8)和glycerol-1- monooleate(9)。结论 化合物5为新的天然产物,其余化合物均为已知化合物。生物活性测试结果显示化合物1和2对火龙果黑斑病菌有一定的抑制作用。     

Cytotoxic constituents fromSolanum lyratum

Activity-guided fractionation of the ethanol extract of the whole plant from Solanum lyratum resulted in the isolation of a new pregnane derivative glycoside, 16-dehydropregnenolone 3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranosid uronic acid (2), as well as other six known compounds: 16-dehydropregnenolone (1), allopregenolone (3), protocatechuic acid (4), vanillic acid (5), caffeic acid (6), and scopoletin (7). The structures of the isolated compounds were elucidated on the basis of their spectral data and chemical evidences. Compounds 1, 3, 4 were isolated for the first time from this plant. Cytotoxic activities of the isolated compounds were evaluated. Compound 1 exhibited significant cytotoxic activity against A375-S2, HeLa, SGC-7901, and Bel-7402 with IC50 values of 13.1 +/- 0.9, 21.5 +/- 1.0, 40.2 +/- 0.7, and 49.8 +/- 1.2 microg/mL, respectively.     

延龄草化学成分的分离与鉴定

目的研究延龄草(Trillium tschonoskii Maxim.)根及根茎的化学成分。方法利用硅胶柱色谱、凝胶柱色谱、ODS(反相C18键合硅胶)柱色谱、高效液相色谱等色谱技术对延龄草的化学成分进行分离和纯化,通过理化性质和NMR等波谱数据分析确定化合物的结构。结果从延龄草根及根茎70%(φ)乙醇提取物中分离得到11个已知成分,分别确定为重楼皂苷Ⅵ(chonglouosideⅥ,1)、偏诺皂苷元-3β-O-α-L-吡喃鼠李糖基-(1→4)-[O-α-L-吡喃鼠李糖基-(1→2)]-O-β-D-吡喃葡萄糖苷(penogenin-3β-O-α-L-rhamnopyranosyl-(1→4)-[O-α-L-rhamnopyranosyl-(1→2)]-O-β-D-glu-copyranoside,2)、重楼皂苷Ⅶ(chonglouosideⅦ,3)、偏诺皂苷元-3β-O-β-D-吡喃葡萄糖基-(1→6)-[O-α-L-吡喃鼠李糖基-(1→2)]-O-β-D-吡喃葡萄糖苷(trikamsteroside B,4)、(25S)-27-羟基偏诺皂苷元-3β-O-α-L-吡喃鼠李糖基-(1→2)-O-β-D-吡喃葡萄糖苷((25S)-27-hydroxypenogenin-3β-O-α-L-rhamnopyranosyl-(1→2)-O-β-D-glucopyranoside,5)、(25S)-27-羟基偏诺皂苷元-3β-O-α-L-吡喃鼠李糖基-(1→4)-[O-α-L-吡喃鼠李糖基-(1→2)]-O-β-D-吡喃葡萄糖苷((25S)-27-hydroxypenogenin-3β-O-α-L-rhamnopyranosyl-(1→4)-[O-α-L-rhamnopyranosyl-(1→2)]-O-β-D-glucopyranoside,6)、(25S)-27-羟基偏诺皂苷元-3β-O-α-L-吡喃鼠李糖基-(1→4)-O-α-L-吡喃鼠李糖基-(1→4)-[O-α-L-吡喃鼠李糖基-(1→2)]-O-β-D-吡喃葡萄糖苷(polyphyllosideⅢ,7)、(23S,24S,25S)-螺甾-5-烯-1β,3β,21,23,24-五羟基-1β-O-β-D-呋喃芹糖基-(1→3)-O-α-L-吡喃鼠李糖基-(1→2)-[O-β-D-吡喃木糖基-(1→3)]-O-α-L-吡喃阿拉伯糖苷(trikamsteroside E,8)、3β-O-α-L-rhamnopyranosyl-(1→2)-O-β-D-glucopyranosylhomo-aro-cholest-5-ene-26-O-β-D-glucopyranoside(parispseudoside B,9)、3β-O-α-L-rhamnopyranosyl-(1→4)-[O-α-L-rhamnopyranosyl-(1→2)]-O-β-D-glucopyranosylhomo-aro-cholest-5-ene-26-O-β-D-glucopyranoside(aethioside A,10)、3β-O-α-L-rhamnopyranosyl-(1→4)-O-α-L-rham-nopyranosyl-(1→4)-[O-α-L-rhamnopyranosyl-(1→2)]-O-β-D-glucopyranosylhomo-aro-cholest-5-ene-26-O-β-D-glucopyranoside(parispseudoside A,11)。结论化合物9-11为首次从百合科植物中分离得到,7为首次从延龄草属植物中分离得到,4-6、8为首次从延龄草中分离得到。     

Triterpene saponins from Lysimachia christinae

Seven triterpene saponins were isolated from Lysimachia christinae and identified as lysichriside A (1), lysichriside B (2), primulanin (3), lysikokianoside 1 (4), anagallisin C (5), ardisiacrispin A (6), and ardisicrenoside B (7). Compounds 1 and 2 are new triterpene saponins, compounds 3, 5, and 7 were isolated from this genus, and compounds 4 and 6 were isolated from this species for the first time. Their structures were elucidated by means of 1D and 2D NMR experiments.     

Studies on chemical constituents from Ilex pubescens

Two new phenolic glycosides, ilexpubsides A and B, along with four known lignan glycosides were isolated from the roots of Ilex pubescens. By spectral evidence, the structures of the new compounds were elucidated as 4-O-β-D-[6'-O-(4'-O-β-D-glucopyranosylvanilloyl)glucopyranosyl] vanillic acid (1) and syringinic 6'-O-β-D-xylopyranoside (2). The known compounds were identified to be liriodendrin (3), (-)-olivil (4), tortoside A (5) and (+)-cyclo-olivil (6). All compounds were first isolated from Ilex pubescens.     

蜘蛛香的化学成分研究

目的：研究蜘蛛香Valeriana jatamansi的化学成分。方法：通过各种色谱手段（Silica gel、ODS、HPLC）对蜘蛛香甲醇提取物进行分离纯化,通过^1H—NMR、^13C-NMR等波谱技术确定化合物的结构。结果：分离并鉴定了4个含氯元素的环烯醚萜类化合物,分别为rupesin B（1）、chlorovaltrate（2）、volvaltrates B（3）、1,5-dihydroxy-3．8-epoxyvalechlorine A（4）。结论：化合物1～3为首次从植物蜘蛛香中分离得到。     

辽东楤本化学成分的研究

从辽东楤本(Aralia elata)的根皮中分得8个化合物,利用理化和光谱方法鉴定分别为胡萝卜甙-6’-棕榈酸酯(6’-O-palmitoyl-β-sitosterol-3-O-β-D-glucoside,A₅)、罗盘草甙A(silphioside A,A₉)、楤木皂甙A甲酯(araloside A methyl ester,A₁₀)、竹节人参甙I_b(chikusetusaponin I_b,A₁₁)、楤木皂甙A(araloside A,A₁₂)、楤木皂甙C(araloside C,A₁₅、楤木皂甙G(araloside G,A₁₆)、无梗五加甙D(acanthoside D,B₁)。化合物A₅,A₉,A₁₁和B₁为首次从该植物中分得,A₁₀为新天然产物,A₁₆为新化合物命名为楤木皂甙G,归属了化合物A₉,A₁₅的¹³C-NMR化学位移。     

平卧菊三七叶化学成分的分离与鉴定

目的研究平卧菊三七叶子的化学成分。方法采用硅胶柱色谱法、反相ODS柱色谱法、Seph-adex LH-20柱色谱法和HPLC法等进行分离纯化,并通过波谱分析技术鉴定其化学结构。结果从平卧菊三七叶子中分离得到7个化合物,分别鉴定为β-谷甾醇-3-O-龙胆二糖苷(β-sitosterol-3-O-gentiobioside,1)、3-吲哚甲酸(indole-3-carboxylic acid,2)、蚱蜢酮(grasshopper ketone,3)、β-谷甾醇(β-sitosterol,4)、毒豆甲酮(drummondone A,5)、正三十二烷醇(n-dotriacontanol,6)和毒豆乙酮(drummondone B,7)。结论化合物1～3、5、7为首次从菊三七属植物中分离得到,化合物4、6为首次从平卧菊三七中分离得到。