应用ReCell技术移植治疗稳定期白癜风的临床疗效观察

 目的 观察ReCell技术移植治疗稳定期白癜风的临床疗效。方法 选择2015-07至2017-07稳定期白癜风患者21例,黑素细胞来源采用正常皮肤发疱的方法,应用ReCell技术将疱壁制成含有黑素细胞的表皮细胞混悬液,将表皮细胞混悬液种植于白斑皮损创面,移植后观察临床治疗效果。结果 21例白癜风患者中的33块白癜风皮损进行了移植治疗,移植后皮损均出现程度不等的复色,移植后6个月的3、4级复色率达87.09%,移植后未见不良反应。结论 应用ReCell技术移植治疗稳定期白癜风,具有移植区复色效果好、供皮区不留瘢痕的优点,并且临床上安全性可靠,操作相对简单,不需要专门的实验室,值得临床推广应用。     

白癜风患者正常表皮黑素细胞培养与生物学功能的研究

目的：进一步研究和探索白癜风患者正常表皮黑素细胞培养和生物学功能，为白癜风移植治疗提供实验依据。方法：从白癜风患者的皮肤发疱壁上获取黑素细胞，应用M2培养基进行纯黑素细胞培养与增殖，研究人黑素细胞体外培养后生物学功能。结果：10例白癜风患者自体黑素细胞培养成功率为100％，培养后获得的黑素细胞数量理想，生物学功能可靠。结论：在M2培养基条件下进行纯黑素细胞培养增殖，获得的黑素细胞数量比较理想，其生物学功能可靠，可以进一步应用于白癜风的移植治疗。     

窄谱中波紫外线联合自体表皮移植治疗稳定期白癜风

目的了解单纯采用窄谱中波紫外线照射与自体表皮移植术联合术前术后NB-UVB照射治疗白癜风的临床疗效差异。方法对60例局限型稳定期白癜风患者皮损采用单纯NB-UVB照射与自体表皮移植术联合术前术后NB-UVB照射治疗白癜风的方法,随访观察6个月,按痊愈、显效、有效、无效和复发病例数进行统计分析。结果随访6个月,单用NB-UVB照射组总有效率为50%,自体表皮移植术联合NB-UVB照射治疗组总有效率为83.33%;后者显著高于前者（χ2=7.5,P=0.006,P﹤0.01）。结论自体表皮移植术前术后联合NB-UVB照射提高了治疗白癜风疗效,缩短治疗时间。     

两种黑素细胞种植方法在治疗白癜风中的对比分析

目的:研究和分析自体黑素细胞培养后应用两种不同的移植方法治疗白癜风的临床效果。方法:采用从疱壁上获取黑素细胞、纯黑素细胞培养与增殖,应用移植区疱内注射法和刮除种植法两种方法进行移植治疗白癜风。结果:移植区疱内注射法和刮除种植法移植治疗白癜风的评分分别为6.36±1.43和10.24±1.35,有显著性差异(P<0.01)。结论:应用刮除种植法进行自体黑素细胞培养后移植治疗白癜风,操作方法较简单,移植治疗面积大,色素分布均匀,临床效果更好。     

负压吸疱法及薄层削片法白癜风自体表皮移植疗效观察

目的探讨负压吸疱法及薄层削片法自体表皮移植治疗白癜风的疗效观察。方法将86例患者按取皮方法不同分为薄层削片组42例及负压吸疱组44例,观察自体表皮移植后的疗效。结果术后8周,薄层削片法自体表皮移植的总有效率明显高于负压吸疱法(P<0.01)。结论薄层削片法自体表皮移植治疗白癜风较负压吸疱法简便有效。     

自体表皮细胞移植治疗白癜风疗效观察

目的 评价自体表皮细胞移植治疗白癜风的疗效。方法 对136例白癜风患者用自体表皮细胞移植方法治疗后随访半年结果分析。结果 痊愈 12例(8.8％),显效104例(76.5％),有效18例(13.2％),无效2例(1.5％)。总有效率90％以上。结论 自体表皮细胞移植治疗白癜风操作简单,疗效显著。     

抗凋亡在白癜风治疗中的意义

白癜风是一种常见的皮肤色素脱失性疾病,大量资料表明,白癜风患者表皮皮损处存在黑色素细胞缺失。组织病理学证据表明,白癜风皮损处黑素细胞消失或减少[1],白癜风患者黑素细胞破坏机制目前还不明确,但组织病理学以及一些实验室证据表明,不能除外凋亡在白癜风的黑素细胞破坏过程中发挥了重要作用[2]。组织病理学观察发现,白癜风患者皮损处黑     

自体表皮细胞移植治疗白癜风疗效观察

目的：评价自体表皮细胞移植治疗白癜风的疗效。方法：对136例白癜风患用自体表皮细胞移植方法治疗后随访半年结果分析。结果：痊愈12例（8．8％）,显效104例（76．5％）,有效18例（13．2％）,无效2例（1．5％）。总有效率90％以上,结论：自体表皮细胞移植治疗白癜风操作,疗效显。     

加温负压吸疱自体表皮移植法治疗白癜风

加温负压吸疱自体表皮移植法治疗白癜风杜华★陈起科★陈婷荣★刘杜姣★王洁★关键词白癜风自体表皮移植色素细胞中国图书资料分类法分类号Ｒ７５８．４１白癜风以局限性或泛发性皮肤色素脱失为特征，目前对本病治疗方法虽然很多，但疗效多不理想。我们于１９９５年开始用...     

自制细胞分离器提取表皮细胞治疗白癜风36例

白癜风为皮肤科常见病，病因不明，治疗棘手。目前，国内外医学界普遍认为表皮细胞移植术治疗白癜风疗效较好，尤其对局限性、稳定期的病变，疗效更显著。1999开始，我们应用自制表皮细胞分离器提取含有黑素细胞的表皮移植，在门诊治疗白癜风36例，疗效良好。     

寻常型白癜风皮损的超微结构研究

用透射电镜对１０例寻常型白癜风患者皮损进行了超微结构研究。白殿风皮损存在着黑素细胞、角朊细胞、郎汉斯细胞和基底膜病变化,以皮损边缘处明显。３例标本的表皮有类似于淋巴细胞的单一核细胞浸润,其中１例单一核细胞与郎汉斯细胞紧密并列。一直真皮树枝状细胞与淋巴细胞相接触,并向表皮移行,显示白癜风病理变化类似于变态反应性接触性皮炎,提示郎汉期细胞有大癜风发病机制中起着重要的作用。     

白癜风患者血清抗黑素细胞抗体的免疫荧光检测

目的建立抗黑素细胞抗体免疫荧光检测方法,测定白癜风患者血清中抗黑素细胞抗体。方法取外科手术后人眼睑正常皮肤,用胰蛋白酶消化后制成细胞悬液,在M2黑色素细胞选择性培养基中培养,每周传代1次,经4～5次传代,以免疫荧光和多巴胺染色检测培养细胞的纯度和生物学特性,转移到明胶包被的玻璃片上生长至70％融合,丙酮固定后,与不同稀释度的白癜风患者和正常对照血清反应,加入FITC标记的兔抗人抗体,观测荧光染色效果。结果黑素细胞能在培养基中选择性生长,经4～5W培养获得纯的黑素细胞,无成纤维细胞和角质形成细胞的污染。19例发展期患者抗黑素细胞抗体阳性15例,抗体阳性率79％;10例高于1：50,5例抗体滴度为1：10。稳定期15例中,抗黑素细胞抗体阳性4例,抗体阳性率27％。结论建立了抗黑素细胞抗体免疫荧光检测方法,白癜风患者血清中有抗黑素细胞抗体,且其滴度与病情发展相关。     

游离植皮的生物学治愈过程

本文重点阐述了游离植皮的皮肤色泽变化。如果移植皮片与受区周围的正常皮肤相似,术后两者间不会出现明显的色泽差异;而从非暴露部移植到暴露部的皮肤,其色泽有明显的差异,但多有随季节性变动的倾向,而脚掌部皮肤移植到手掌时色泽不会发生变化。经电镜观察,移植皮肤的黑色素细胞亦呈规律性变化。同时对移植皮肤的收缩及挛缩、硬化、感觉恢复和皮肤器官的功能重建也进行了讨论。     

The use of perfusion CT for the evaluation of therapy combining AZD2171 with gefitinib in cancer patients

The purpose of this study was to determine the feasibility of dynamic contrast–enhanced perfusion CT (CTP) in evaluating the hemodynamic response of tumors in the chest and abdomen treated with a combination of AZD2171 and gefitinib. Thirteen patients were examined just before and every 4-6 weeks after starting therapy. Following intravenous injection of a contrast agent, dynamic image acquisition was obtained at the level of a selected tumor location. To calculate perfusion, the maximum-slope method was used. Pre-treatment average perfusion for extra-hepatic masses was 84 ml/min/100 g, for liver masses arterial perfusion was 25 ml/min/100 g, and a portal perfusion of 30 ml/min/100 g was found. After the administration of AZD2171 and gefitinib, in extra-hepatic masses an initial decrease in perfusion of 18% was followed by a plateau and in liver masses an initial decrease of 39% within the lesions and of 36% within a rim region surrounding the lesions was followed by a tendency to recovery of hepatic artery flow. In conclusion, CTP is feasible in showing changes of perfusion induced by anti-angiogenic therapy.     

308nm准分子激光治疗白癜风现状

308 nm准分子激光是近年来治疗白癜风的一种新型激光治疗手段,具有快速、有效、不良反应少等优点。单独应用308nm准分子激光具有更好的疗效,联合外用药物及自体表皮移植治疗白癜风效果比单独应用更显著。其疗效与患者的性别、年龄等没有明显的关联,但与皮损部位、治疗次数、皮肤类型等有明显的相关性,而与疾病病程的关系尚有争议。     

Lesions of the brain stem: assessment by magnetic resonance imaging

Summary Ninety-six magnetic resonance imaging (MRI) studies demonstrating solitary brain stem lesions were reviewed in order to establish distinguishing features between tumours and other lesions. Histological confirmation of the radiological diagnoses was obtained in 33% of patients. The morphology of the lesion rather than its signal characteristics was the most useful feature in differential diagnosis, except after haemorrhage. However, areas of abnormal T2 recovery time were significantly more extensive than areas of abnormal T1 recovery time in tumours at presentation. In other lesions and in tumours following radiotherapy induced regression, the extent of abnormal T1 and T2 signal was the same. Possible reasons for this observation are discussed. The accuracy of computed tomography (CT) and MRI were similar for lesions causing brain stem expansion but for small lesions MRI was more sensitive and provided better topographical information.     

320 nm强脉冲光与窄谱中波紫外线治疗稳定期白癜风近期疗效的比较

目的比较320 nm强脉冲光与窄谱中波紫外线治疗稳定期白癜风的近期疗效。方法稳定期白癜风患者103例,随机分为2组,治疗组50例给予320nm强脉冲光治疗,能量密度360 J/cm~2,之后递增30～80J/cm~2,光斑面积1.5 cm×4.0 cm,脉宽30～50 ms,每月治疗1次;对照组53例给予窄谱中波紫外线照射皮损,能量密度0.3～4.0J/cm~2,3 d照射1次。照射后两组患者均口服复合维生素B、叶酸,外用复方卡力孜然酊。3个月为1个疗程,根据色素恢复的程度评价临床疗效。结果痊愈率治疗组(第1疗程为18.0%,第2疗程为64%)显著高于对照组(第1疗程为5.7%,第2疗程为13.2%)(均P0.001),显效率治疗组第1疗程(38.0%)显著高于对照组第1疗程(24.5%)(P0.01);治疗组病程1年者疗效显著优于病程1年者(显效率第1疗程病程1年者48.5%,病程1年者为17.7%,P0.001)。痊愈率第2疗程治疗组为69.7%,对照组为13.2%(P0.05);对不同部位皮损统计学分析的结果显示,以头面部疗效最好,躯干、四肢次之,手足较差。结论 320 nm强脉冲光治疗稳定期白癜风疗效明显优于窄谱中波紫外线,起效快,不良反应少,是治疗稳定期白癜风患者比较理想的选择。     

Laser-fluorescence assessment of sodium hypochlorite quenching reversal agents on human dentine

BackgroundLaser-fluorescence diagnostic technology for real-time clinical assessment of residual bacteria could help assist in determining the endpoints for root canal debridement. Sodium hypochlorite (NaOCl) can however quench fluorescence and lead to false low reading. This study aims to evaluate various antioxidant for their ability to recover quenched fluorescence in dentine treated with NaOCl.MethodsHuman dentine fluorescence was measured using 655 nm laser at baseline and again after a 2 min application of 4% NaOCl. The putative recovery agents were then applied, and the fluorescence measured after 5, 10, 20, 30 and 60 min. Recovery from quenching was also assessed using laser confocal scanning microscopy (CLSM) with a bound tetracycline fluorophore using 488 nm excitation.ResultsA 5 min application of vitamin E oil or buffered 2% lignocaine solution (1:80,000 adrenaline) was effective in regaining quenched fluorescence within the following 5 mins. Distilled water, sodium thiosulfate, unbuffered 2% lignocaine with 1:80000 adrenaline and phosphate buffered saline were less effective, and of equal performance. Ascorbic acid and butylated hydroxyanisole were not effective and had deleterious effects on the levels of dentine fluorescence. CLSM provided confirmation of recovery from quenched fluorescence using vitamin E oil.ConclusionBased on these findings, reversal agents should be employed when assessing the fluorescence of dentine that has been exposed to NaOCl or other quenching agents.