胃力康颗粒对实验性胃溃疡大鼠胃组织碱性成纤维细胞生长因子表达的影响

[目的]观察胃力康颗粒对实验性胃溃疡大鼠胃组织碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)表达的影响,探讨胃力康颗粒的疗效机制.[方法]大鼠随机分成正常组、模型组、法莫替丁对照组和胃力康治疗组,以Okabe改良法复制实验性胃溃疡大鼠模型,采用RT-PCR检测各组大鼠胃组织bFGF mRNA表达水平.[结果]胃力康治疗组bFGF mRNA表达水平较模型组明显升高,差异有统计学意义(P＜0.05).[结论]胃力康颗粒能上调bFGF mRNA的表达,从而促进溃疡愈合.     

怀山药多糖对大鼠胃溃疡的疗效及胃组织碱性成纤维细胞生长因子水平的影响

[目的]观察怀山药多糖对胃溃疡大鼠的治疗作用,研究怀山药多糖对胃溃疡大鼠胃组织碱性成纤维细胞生长因子（bFGF）水平的影响。[方法]将50只大鼠随机分为5组,分别为空白组、模型组、怀山药多糖组、替普瑞酮组和怀山药多糖＋替普瑞酮组。观察并比较各组胃溃疡的愈合情况,取胃组织匀浆检测并比较bFGF水平。[结果]怀山药多糖组的溃疡指数显著低于模型组（P〈0.01）;怀山药多糖组的胃组织bFGF水平显著高于空白组（P〈0.01）和模型组（P〈0.05）。[结论]怀山药多糖具有良好的胃黏膜保护作用,其机制可能与上调胃溃疡大鼠胃组织bFGF水平有关。     

成纤维细胞生长因子促自身血管搭桥的研究进展

“自身血管搭桥”是人们正在探索的治疗冠心病的新战略。本文就成纤维细胞生长因子促“自身血管搭桥”的研究进展做一综述。     

碱性成纤维细胞生长因子治疗脑梗死研究进展

大量实验证明，脑梗死后外源性碱性成纤维细胞生长因子（ｂ Ｆ Ｇ Ｆ） 能显著缩小梗死组织体积，促进感觉运动功能的恢复。ｂ Ｆ Ｇ Ｆ 治疗脑梗死可能具有很好的临床应用前景。     

成纤维细胞生长因子与神经再生

成纤维细胞生长因子与神经再生广州暨南大学（５１０６３２）席保平，朱家恺成纤维细胞生长因子（简称ＦＧＦ）可刺激成纤维细胞分裂增殖，故称为成纤维细胞生长因子。研究表明，ＦＧＦ也可刺激大量中胚层和神经外胚层来源细胞生长。因而对血管生成、肢体发育、伤口愈合起...     

成纤维细胞生长因子与脑缺血

成纤维细胞生长因子(fibroblast growth factor,FGF)与脑血管病关系的研究始于90年代。缺血后,脑组织中FGF水平增加,不仅星形细胞内FGF和FGF mRNA表达增加,而且神经元中FGF和FGF mRNA表达也增加。外源性aFGF和bFGF有一定程度的对抗缺血性脑损害的作用。     

抗碱性成纤维细胞生长因子对家兔实验性矽肺发…

采用抗碱性成纤维细胞生长因子（ｂＦＧＦ）血清进行家兔实验性矽肺的治疗。结果发现：９０天的实验组矽结节比对照组明显减少减小，对照组矽结节以成纤维细胞为主夹杂少量胶原纤维，而实验组仍停留在以巨噬细胞为主的细胞性矽结节阶段；至１８０天，对照组矽结节主要由胶原纤维组成，而实验组矽结节则仍以巨噬细胞为主；体外小鼠胚胎肺成纤维细胞培养，结果显示ｂＦＧＦ明显促进细胞生长，而抗ｂＦＧＦ血清则抑制其生长，与上述体内     

酸性成纤维细胞生长因子的神经保护作用

脑、脊髓神经细胞受损后，常常不能重建正确的树突和轴突联系，即使经过治疗，患者的预后仍不理想。大量的研究证实，神经系统损伤后各类生长因子、神经营养因子（NTF）可促进神经元存活和轴突再生。目前，国内外的研究主要集中在NTF、碱性成纤维细胞生长因子（bFGF）和酸性成纤维细胞生长因子（aFGF）上。近10年来，成纤维细胞生长因子的神经保护作用已得到确认，作者就aFGF在神经系统中的作用综述如下。     

成纤维细胞生长因子21对心肌缺血的保护作用

成纤维细胞生长因子21作为新发现的成纤维细胞生长因子家族的一员,对糖脂代谢、胰岛素分泌,以及心血管疾病具有重要的调节作用.近期的研究发现,心肌缺血可诱导成纤维细胞生长因子21的表达和分泌,从而发挥其对心肌细胞的保护作用.现着重于成纤维细胞生长因子21在心血管疾病,尤其是在心肌缺血中的保护作用做一综述.     

Mucosal concentration of basic fibroblast growth factor in the healing process in human giant gastric ulcers

OBJECTIVES: Basic fibroblast growth factor (bFGF) is a key factor in the healing of human and experimental peptic ulcers, but the behavior of bFGF in human giant gastric ulcer remains to be determined. We determined the bFGF content in the rim of giant ulcers (bFGF rim) and in non-ulcerated mucosa located opposite the ulcer (bFGF opposite) before and during treatment. METHODS: Biopsy specimens were endoscopically obtained from 31 patients with giant gastric ulcers and 17 patients with small ulcers before and 2, 4 and 8 weeks after treatment. The bFGF concentrations in the specimens were measured using a sandwich enzyme immunoassay. RESULTS: Before treatment, the bFGF rim and bFGF opposite concentrations were not associated with ulcer size. The bFGF rim concentration before treatment in the rapid healing group was higher than that in the slow healing group, but no significant difference in bFGF opposite concentrations were found between the two groups. The bFGF rim concentration in the rapid healing patients decreased during treatment, while the slow healing patients showed an inverse response. The bFGF opposite concentration did not change during treatment and bFGF rim concentrations in Helicobacter pylori-positive stomachs were significantly lower than those in H. pylori-negative stomachs. CONCLUSIONS: The bFGF rim concentration is not involved in the formation of giant gastric ulcers in humans. However, the bFGF rim concentration does appear to promote healing. The bFGF opposite concentration is not related to either the formation or healing of giant gastric ulcers.     

Omentum and basic fibroblast growth factor in healing of chronic gastric ulcerations in rats

Omentum was shown to exhibit angiogenic activity, but its role in healing of chronic gastric ulcers is unknown. This study was designed to compare the effects of omentum and basic fibroblast growth factor (bFGF), a potent angiogenic factor, on healing of chronic gastric ulcers in rats. Several series of rats with gastric ulcers were used: series A with intact omentum (control), series B with omentum resected, and series C with omentum placed on the serosal side of the ulcer. Series A–C were divided into four groups treated with vehicle (I); indomethacin (II), an inhibitor of prostaglandin formation, difluoromethylornithine (DFMO) (III); an inhibitor of polyamine biosynthesis or bFGF (IV). Seven days after ulcer induction, the animals were anesthetized, the gastric blood flow (GBF) was determined by laser Doppler flowmetry (LDF), and the ulcer area was measured by planimetry. Biopsy samples of the ulcer margin were taken for determination of the number of capillaries and myofibroblasts in the granulation tissue. Attachment of omentum significantly accelerated ulcer healing, whereas omentectomy delayed this process. LDF revealed the decrease in the GBF at the ulcer margin to 45% and at the ulcer bed to 18% of the value recorded in the intact adjacent mucosa. Attachment of the omentum significantly increased the blood flow at the ulcer margin and increased the number of capillaries and myofibroblasts in the granulation tissue. Indomethacin (1 mg/kg/day) that inhibited mucosal PGE₂ by about 85% delayed significantly ulcer healing without affecting the blood flow in the ulcer area. DFMO (200 mg/kg intraperitoneally) suppressed ODC activity in the mucosa but did not influence the ulcer healing. bFGF given subcutaneously accelerated dose-dependently ulcer healing and stimulated angiogenesis to a similar extent as the attached omentum. We conclude that omentum enhances the ulcer healing in similar way as bFGF and that this effect is accompanied by the increased angiogenesis and blood flow in the ulcer area.     

胃癌组织与血清中VEGF和bFGF的表达意义

目的：研究胃癌患者血清和组织中VEGF,bFGF的表达与胃癌临床特征之间的关系,研究二者的相关性及组织和血清之间的相关性,探讨VEGF,bFGF在胃癌的发生、发展、侵袭和转移中的作用方法：应用酶联免疫技术(ABC-ELISA方法)检测73例胃癌患者术前血清和20例健康体检者血清中的VEGF,bFGF的表达水平,同时应用免疫组织化学染色方法检测癌组织和癌旁组织中VEGF,bFGF的表达．结果：胃癌患者术前血清VEGF,bFGF表达水平均明显高于健康体检者(YEGF：101．8±53．3 ng/L vs 16．1±22．5ng/L,P＜0．05；bFGF：152．9±42．7ng/L vs 25．0±11．4ng/L,P＜0．05)．胃癌患者术前血清VEGF,bFGF的表达水平均随胃癌的浸润深度、TNM分期、淋巴结转移、远处转移而增高(P＜0．05),而与年龄、性别及病理类型无关．胃癌组织VEGF的阳性表达率为71．2%,癌旁组织中VEGF均未见阳性表达,二者之间有显著性差异(X~2=32．1,P＜0．05)；胃癌组织中bFGF的阳性表达率为63．0%、癌旁组织中bFGF阳性表达率为(10%),二者之间亦有显著性差异(X~2=17．7,P＜0．05)．胃癌患者组织VEGF,bFGF的表达水平均与胃癌的浸润深度、TNM分期、淋巴结转移、远处转移有关(P＜0．05),而与年龄、性别及病理类型无关．胃癌患者血清VEGF的表达水平与血清bFGF的表达水平呈明显正相关(r=0．439,P＜0．01),胃癌患者组织VEGF的表达水平与组织bFGF的表达水平呈明显正相关(r=0．391,P＜0．01)；胃癌患者术前血清VEGF的表达水平与组织VEGF的表达呈正相关(r=0．346,P＜0．01),术前血清bFGF的表达水平与组织bFGF的表达呈正相关(r=0．304,P＜0．01),均有显著性差异．结论：VEGF,bFGF在胃癌的发生、发展、转移及预后起着重要的作用,有望成为胃癌术前诊断、术后随访、复发转移监测、评价抗血管生成药物疗效和化疗效果判定的新的肿瘤标志物．     

Effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing in rats

AIM: To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing. METHODS: Male Spraque-Dawley rats (n=48) were divided into four groups. Groupl served as control group, group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-αand IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid. RESULTS: On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly (P<0.05) increased in the ulcer groups when compared to the control group. The level of TNF-αwas elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera, leukocyte adherence was reduced in postcapillary venule. The level of IL-10 was elevated, but the level of TNF-αhad no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-αwere still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-αlevel. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment. CONCLUSION: Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-αlevel and reduce IL-10 level. Aloe vera treatment can reduce leukocyte adherence and TNF-αlevel, elevate IL-10 level and promote gastric ulcer healing.     

幽门螺杆菌与胃黏膜bFGF,FGFR-2表达的关系及意义

目的:探讨幽门螺杆菌(H pylori)感染的胃黏膜上皮细胞碱性成纤维细胞生长因子(bFGF)、成纤维细胞生长因子受体-2 (FGFR-2)的表达及其在胃黏膜癌变过程中的意义.方法:选择慢性浅表性胃炎(CSG)30例、胃黏膜肠上皮化生(IM)29例、不典型增生(Dys) 31例及胃癌(GC)55例.采用免疫组化SP法检测胃黏膜上皮细胞bFGF,FGFR-2表达状况,用快速尿素酶试验和组织学Warthin-Starry嗜银染色法联合检测胃黏膜H pylori感染情况.结果:CSG组bFGF,FGFR-2的表达显著低于其余三组(IM组:χ~2=4.002,P<0.05;χ~2= 4.163,P<0.05;Dys组:χ~2=15.779,P=0.000;χ~2=15.949,P=0.000;GC组:χ~2=24.110,P= 0.000;χ~2=18.736,P=0.000),IM组的表达低于Dys组及GC组(Dys组:χ~2=4.258,P<0.05;χ~2 =4.212,P<0.05:GC组:χ~2=7.786,P<0.01;χ~2 =4.687,P<0.05),而Dys组与GC组间无显著性差异.H pylori阳性IM及Dys组bFGF,FGFR-2的表达均显著高于阴性组(IM组:χ~2=10.076,P<0.01;χ~2=7.535,P<0.01;Dys组:χ~2=11.501,P<0.01;χ~2=8.330,P<0.01).H pylori阳性Dys组bFGF,FGFR-2表达显著高于GC组(χ~2= 4.201,P<0.05;χ~2=3.982,P<0.05),H pylori阳性IM组则与GC组的表达无显著性差异;H pylori阴性Dys组及IM组bFGF的表达均显著低于GC组(χ~2=5.736,P<0.05;χ~2=17.113,P= 0.000),H pylori阴性Dys组FGFR-2表达与GC组无显著性差异而IM组的FGFR-2的表达显著低于GC组(χ~2=11.091,P<0.05).结论:H pylori感染引起胃黏膜上皮细胞bFGF及FGFR-2的过度表达可能与H pylori感染致胃黏膜上皮细胞的癌变有关.     

乙酰肝素酶反义寡核苷酸对人胃癌细胞株SGC7901表达bFGF的抑制作用

目的 探讨乙酰肝素酶反义寡核苷酸(AS-ODN)对人胃癌细胞株SGC7901表达碱性成纤维细胞生长因子(basic fibrob-last growth factot，bFGF)的影响。方法 AS-ODN和乙酰肝素酶mRNA起始密码子区互补，无义寡核苷酸(NS-ODN)为对照组，用阳离子脂质体包裹ODNs并转入SGC7901细胞；转染后48小时提取细胞总RNA，半定量RT-PCR法检测乙酰肝素酶基因的含量，免疫细胞化学法检测bFGF的表达。结果 AS-ODN处理后SGC7901表达乙酰肝素酶mRNA下降；处理前bFGF、的表达率为72．23％，不同浓度(0．1、0．2、0．4、0．8μmoL／L）AS-ODN处理后bFGF的表达率下降程度不同(分别为57．15％、51．11％、42．36％和40．25％)。结论 和乙酰肝素酶mRNA起始密码子区互补的AS-ODN对SGC7901表达bFGF有明显影响，且呈剂量相关性。     

Three cases of ischemic ulcer due to arteriosclerosis obliterans responding to basic fibroblast growth factor spray

The management of the chronically ischemic leg with ulcer formation that is not suitable for either surgical or interventional treatment is still a matter of controversy. We describe three cases of ischemic ulcer treated with basic fibroblast growth factor spray. Ulcer healing was accelerated and complete epithelialization was achieved in all cases. Basic fibroblast growth factor spray is useful in the treatment of the ischemic ulcer in patients with arteriosclerosis obliterans, especially in high-risk surgical patients.     

Effect of early administration of exogenous basic fibroblast growth factor on acute edematous pancreatitis in rats

AIM: To observe the therapeutic effect of early administration of exogenous Basic fibroblast growth factor (bFGF) on acute edematous pancreatitis (AEP) in rats. METHODS: Thirty male Sprague-Dawley rats were randomly divided into three (n = 10): normal control group (groupⅠ), AEP group (groupⅡ) and AEP with bFGF treatment group (groupⅢ). AEP was induced by subcutaneous injection of cerulein (5.5μg/kg and 7.5μg/kg) at 1 h interval into rats of groupsⅡandⅢ. Three hours after induction of AEP, 100μg/kg bFGF was administrated intraperitoneally for 1h to groupⅢrats. For test of DNA synthesis in acinar cells, 5-bromo-2'-deoxyuridine (BrdU) labeling solution was intraperitoneally injected into the rats of groupsⅡandⅢ24 h after bFGF treatment. The changes in serum amylase, lipase, pancreatic tissue wet/dry ratio were detected. RESULTS: In bFGF treatment group, there was a significant decrease in the volume of serum amylase, lipase and the pancreatic wet/dry weight ratio(1383.0±94.6 U/L, 194.0±43.6 U/L, 4.32±0.32) compared to AEP group (3464±223.7 U/L, 456±68.7 U/L, 6.89±0.47) (P < 0.01), and no significant difference was found between bFGF treatment and control group (1289±94.0 U/L, 171±23.4 U/L, 4.12±0.26, P > 0.05). The inflammatory changes such as interstitial edema, polymorphonuclear neutrophils (PMNs) and vacuolization were significantly ameliorated compared to AEP group (P < 0.01). A small number of BrdU-labeled nuclei were observed in acinar cells of AEP rats (1.8±0.3 nuclei/microscopic field, n = 10) while diffuse BrdU-labeled nuclei were found in bFGF-treated rats (18.9±1.4 nuclei/microscopic field, n = 10) {P < 0.01). Immunohistochemical study showed increased DNA synthesis in pancreatic acinar cells. CONCLUSION: Early administration of exogenous bFGF has significant therapeutic effect on cerulein-induced acute edematous pancreatitis in rats. Its mechanism is related to the amelioration of inflammation and facilitation of pancreatic regeneration.     

碱性成纤维细胞生长因子在肝细胞癌中的表达及意义

目的 探讨bFGF在肝细胞癌中的表达及其临床病理意义。方法 使用免疫组化SABC法检测41例肝细胞癌手术切除标本及5例外伤性肝破裂肝切取标本。结果 正常对照组bFGF均为阴性。肝细胞癌组bFGF阳性率为70．7％（29／41）。bFGF表达与患者年龄、性别、AFP是否阳性、HBsAg是否阳性无关（P＞0．05）；而与肝癌分化程度、有无癌栓、包膜是否完整有关。在高侵袭转移组与低侵袭转移组间，bFGF阳性率分别为86．4％和52．6％，差异具有显著性（P＜0．05）。结论 bFGF在肝细胞癌中高表达，可以作为判断肝癌恶性程度及转移潜能的参考指标。     

碱性成纤维细胞生长因子促进颌骨缺损修复的实验研究

对24只大白兔造成兔双侧下颌体部15mm×10mm骨缺损,以膨体聚四氟乙烯膜(e-PTFE)覆盖缺损区并与骨膜严密缝合.实验侧(右)注入碱性成纤维细胞生长因子(b-FGF)1000AU,对照侧注入生理盐水.于术后2、4、6、8周各处死一组兔,作X线摄片、组织学观察及钙离子测定,研究各组骨缺损修复速度和愈合程度.结果实验侧术后2周骨断面处有新骨生长并有大量间充质细胞聚集,4周时大量新生骨形成骨小梁向缺损中央延伸,8周完全骨愈合.对照侧在各阶段新骨形成均不如实验侧,且钙离子含量明显低于实验侧.实验证明b-FGF具有明显的诱导成骨作用,可以促进颌骨缺损的修复.