初步探讨口腔鳞状细胞癌中的p33/ING1表达的意义

目的 观察口腔鳞状细胞癌中的p33/ING1基因表达情况,探讨其临床意义.方法 分别选取口腔黏膜正常组织30例、异常口腔黏膜增生组织30例、口腔鳞状细胞癌黏膜组织30例,观察p33/ING1在人正常口腔黏膜上皮角化细胞（Normal Oral Keratinocyte,NOK）、人口腔黏膜上皮异常增生细胞（ Dysplastic Oral Keratinocyte,DOK）和人口腔鳞状细胞癌细胞（Oral Squamous Cell Carcinoma,OSCC）中的表达情况.结果 ING在NOK细胞中的表达要远远高于在OSCC细胞中的表达,有显著性差异和统计学意义（P＜0.05）.结论 p33/ING1蛋白的表达在OSCC细胞中的表达显著下降,p33/ING1表达在口腔鳞状细胞癌的发展中具有重要意义.     

口腔鳞状细胞癌侵袭和转移过程中上皮-间充质细胞转换作用的研究进展

上皮-间充质细胞转换（epithelial—mesenehymaltransition,EMT）是一个动态的、可逆的过程,使细胞从鹅卵石状的上皮表型转向细长的间充质表型。受微环境和很多因素的诱导,EMT参与胚胎发育、慢性炎症、伤口愈合及肿瘤侵袭转移过程。细胞经历EMT后具有多种生物学特性,包括迁移、侵袭、被诱导成干细胞、防止凋亡、防止衰老及抑制免疫等,在肿瘤的转移、复发和耐药中起着关键作用。弄清EMT在口腔鳞状细胞癌（oralsquamouscellcarcinoma,OSCC）中的作用机制,有助于了解OSCC发展的生物学行为,以发现新的生物标志物用于OSCC患者的诊断、靶向治疗和预后判定。     

细胞角蛋白表达在诊断皮肤鳞状细胞癌和基底细胞癌中的应用

目的观察7种细胞角蛋白（Cytokeratin，CK）在皮肤鳞状细胞癌和基底细胞癌中的表达，并探讨其鉴别诊断意义。方法应用免疫组织化学S—P法对41例皮肤鳞状细胞癌．38例基底细胞癌和20例正常皮肤进行Cytokeratin7（K72-2）、Cytokeratin8（C-51）、Cytokeratin109（DE—K10）、Cytokeratin14（LL002）、Cytokeratin17（E3）、Cytokeratin18（D10）、Cytokeratin19（KS19．1）标记，观察不同细胞角蛋白的表达。结果41例皮肤鳞状细胞癌f包括高分化癌36例，中分化癌5例）和38例基底细胞癌中．7种细胞角蛋白的表达和分布有所不同，鳞状细胞癌显示CK10＋／18＋／19-；基底细胞癌为CK10-／18-／19＋。结论有选择地检测一组CK的联合表达．有助于皮肤上皮性肿瘤的鉴别诊断。     

肿瘤抑制蛋白 FBW7在口腔鳞状细胞癌中表达的研究

目的检查肿瘤抑制蛋白FBW7在鳞状细胞癌（ OSCC）中的表达,初步探究FBW7表达与OS-CC细胞克隆形成能力的关系。方法收集19例OSCC患者的癌巢组织及对应癌旁组织,应用免疫组化染色与Real time RT-PCR技术检测FBW7在癌巢及对应癌旁组织中的表达情况。 Western blot 和Real time RT-PCR技术比较口腔鳞状细胞癌细胞系KV、CTST-1、CTST-2以及正常颊黏膜上皮细胞中FBW7蛋白和mRNA表达水平,分析FBW7表达与口腔鳞状细胞癌细胞系克隆形成能力的关系。结果免疫组化和Real time RT-PCR结果显示,FBW7蛋白及mRNA在对应癌旁组织中的表达水平明显高于癌巢组织（P＜0.05）,West-ern blot及Real time RT-PCR结果显示,FBW7蛋白和mRNA在口腔鳞癌细胞系中的表达水平明显低于正常口腔黏膜上皮细胞（ P＜0.05）。克隆形成实验结果表明, FBW7表达较高的舌癌细胞株的克隆形成数目较少,克隆形成能力较弱。结论 FBW7在口腔鳞状细胞癌组织和细胞系中表达显著降低,与口腔癌细胞系的克隆形成能力呈负相关。     

A-NK细胞促口腔鳞状细胞癌凋亡效应的研究

目的：通过观察 A-NK细胞对裸鼠舌鳞状细胞癌移植瘤模型的体外生长特点以及口腔鳞状细胞癌（OS-CC）凋亡指数的检测,探讨 A-NK细胞对 OSCC的免疫治疗机制。方法建立裸鼠舌鳞癌皮下移植瘤模型,随机分为对照组、A-NK细胞组和 NA-NK细胞组,观察33天后瘤重,绘制肿瘤生长曲线;OSCC组织标本经末端脱氧核苷酸转移酶介导的 UTP缺口标记（TUNEL）染色以鉴定凋亡细胞,并由阳性细胞百分比表示细胞凋亡指数（AI）。结果 A-NK细胞组第15天开始肿瘤体积显著小于对照组（P＜0．05）;NA-NK细胞组第27天后肿瘤体积显著小于对照组（P＜0．05）;A-NK细胞组第30天肿瘤体积显著小于 NA-NK细胞组（P＜0．05）,且三组荷瘤裸鼠肿瘤生长重量均有明显差异,差异有统计学意义（P＜0．05）。NK组病例活检癌组织 AI为（0．71±0．66）％,A-NK组织 AI为（1．76±0．83）％,二者间有显著性差异（P＜0．01）;对照组癌组织 AI为（0．34±0．69）％,与前二者间有显著性差异（P＜0．01）。结论 A-NK细胞和 NA-NK细胞均可在裸鼠体内诱导明显的抗肿瘤效应,且 A-NK的抑瘤作用大于 NA-NK细胞。NK细胞对口腔鳞状细胞癌具有免疫杀伤效应,该作用是通过诱导靶细胞凋亡实现的。     

树突状细胞疫苗治疗口腔鳞状细胞癌的研究进展

口腔鳞状细胞癌(oral squamous cell carcinomas,OSCC)的治疗通常选择以手术治疗为主、放化疗相结合的综合治疗模式,使得肿瘤的局部控制率和生存率得到有效地提高.树突状细胞作为免疫系统递呈抗原能力最强的免疫细胞,利用肿瘤特异性抗原冲击树突状细胞制备的疫苗在治疗Ⅲ～Ⅳ期OSCC的研究和应用中取得...     

葡萄糖转运蛋白1在口腔鳞状细胞癌中的表达及意义

口腔鳞状细胞癌（OSCC）是口腔颌面部最常见的恶性肿瘤,其发病率高,约占口腔颌面部恶性肿瘤的80%以上。其肿瘤的发生、增殖、分化、转移都需要大量能量的支持,而葡萄糖是肿瘤的主要能量来源。早在上世纪30年代,Warburg发现肿瘤细胞比正常细胞需要摄取更多的葡萄糖,并且采取糖酵解的方式。葡萄糖转运蛋白1（GLUT-1）是葡萄糖易化转运扩散的最主要蛋白之一,负责葡萄糖转运的基础糖代谢要求。     

口腔鳞状细胞癌中血小板源性生长因子-A及其受体α与血管生成的关系

目的：通过检测人口腔鳞状细胞癌（OSCC）中血小板源性生长因子-A（PDGF-A）及血小板源性生长因子-A 受体-α（PDGFR-α）和血小板-内皮细胞黏附分子（CD31）蛋白标记的微血管密度（MVD）,探讨 PDGF-A 及 PDGFR-α在 OSCC 中的作用及与血管生成的关系,并进一步阐述其临床指导意义。方法收集贵阳医学院附属医院2011年10月至2013年10月手术切除的 OSCC 标本42例（研究组）以及正常口腔黏膜组织11例（对照组）,采用免疫组化（SABC）法分别检测两组的 PDGF-A、PDGFR-α和 CD31表达并计数 MVD。结果（1）PDGF-A 在研究组和对照组中的表达率分别为73.81%、9.09%,而 PDGFR-α则分别为78.57%、9.09%,差异均有统计学意义（均 P<0.05）。（2）在 OSCC 中,PDGF-A 的表达与肿瘤分化程度及是否发生淋巴结转移相关（P<0.05）,而 PDGFR-α仅与肿瘤分化程度相关（P<0.05）。（3）在研究组中, PDGF-A、PDGFR-α的阳性表达者 MVD 值均高于阴性者,差异均有统计学意义（均 P<0.05）。结论 OSCC 中 PDGF-A、PDGFR-α可能通过血管生成参与了肿瘤的生长和转移,但其在口腔鳞癌中的作用机制还有待进一步研究。     

口腔鳞状细胞癌中酪氨酸激酶受体-2的表达与临床病理特征的关系

目的 探索酪氨酸激酶受体-2(Tie-2)与口腔鳞状细胞癌(OSCC)分化程度、颌骨侵犯情况和淋巴结转移情况等临床病理之间的联系。方法 110例OSCC患者标本作为试验组,30例正常患者的口腔黏膜作为对照组,采用免疫组化技术检测Tie-2在OSCC组织与正常口腔组织中的表达;探讨Tie-2在OSCC患者不同临床病理特征中的表达差异。结果 OSCC组Tie-2阳性率显著高于对照组。OSCC血管内皮细胞及癌细胞胞浆中Tie-2为阳性表达,骨旁肿瘤组织的OSCC细胞表达更高。Tie-2在低分化OSCC的表达率明显高于中分化、高分化OSCC。存在淋巴结转移的OSCC中Tie-2表达率较无淋巴结转移的OSCC显著增加,存在颌骨侵犯的OSCC中Tie-2表达率较无颌骨侵犯的OSCC显著增加(P<0.05)。结论 Tie-2在OSCC中高表达,Tie-2在不同OSCC病理分级、是否存在淋巴结转移及颌骨侵犯中存在差异性表达。     

非小细胞肺癌患者外周血三种标志物的检测及临床意义

目的探讨非小细胞肺癌（NSCLC）患者检测血液中癌胚抗原信使核糖核酸（CEA mRNA）、角蛋白19信使核糖核酸（CK19 mRNA）、端粒酶活性（TA）的临床意义。方法以97例NSCLC患者、51例非肿瘤性呼吸系统疾病患者（疾病对照组）和36例健康人（健康对照组）为对象，采集外周血为标本，应用逆转录．套式．聚合酶链反应技术检测CEA mRNA和CK19mRNA；端粒末端重复序列扩增-杂交-酶联免疫检测技术检测TA。结果NSCLC组3种标志物的阳性结果显著高于2个对照组（P〈0．01），且CEA mRNA和TA的特异性和敏感性也显著高于CK19 mRNA（P〈0．01），但不同类型NSCLC检测3个指标结果的差别均无统计学意义（均P〉0．05）。结论检测血液CEA mRNA、CK19 mRNA和TA有助于发现NSCLC细胞血液转移。也有助于预后的评估，且CEA mRNA和TA的价值高于CK19 mRNA，但此3个指标可能无助于鉴别NSCLC的类型。     

Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy

Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers and frequently preceded by non-malignant lesions. Using Shifted-Excitation Raman Difference Spectroscopy (SERDS), principal component and linear discriminant analysis in native tissue specimens, 9500 raw Raman spectra of OSCC, 4300 of non-malignant lesions and 4200 of physiological mucosa were evaluated. Non-malignant lesions were distinguished from physiological mucosa with a classification accuracy of 95.3% (95.4% sensitivity, 95.2% specificity, area under the curve (AUC) 0.99). Discriminating OSCC from non-malignant lesions showed an accuracy of 88.4% (93.7% sensitivity, 76.7% specificity, AUC 0.93). OSCC was identified against physiological mucosa with an accuracy of 89.8% (93.7% sensitivity, 81.0% specificity, AUC 0.90). These findings underline the potential of SERDS for the diagnosis of oral cavity lesions.     

Use of carcinogen-induced premalignant oral lesions in a dendritic cell-based vaccine to stimulate immune reactivity against both premalignant oral lesions and oral cancer

Select groups of premalignant oral lesions carry a high risk of development of secondary premalignant lesions and oral squamous cell carcinoma (OSCC). The goal of the present study was to determine the feasibility of using premalignant lesion-pulsed dendritic cells as a treatment option to prevent development of secondary lesions and development of OSCC. Mice that were treated with the carcinogen 4-nitroquinoline-1-oxide (4NQO) developed premalignant oral lesions and, subsequently, OSCC. Immunohistochemical analyses showed that these 4NQO-induced lesions and OSCC both overexpressed the tumor antigens epidermal growth factor receptor, RAGE and, to a lesser extent, MUC1. Because there was shared overexpression of tumor antigens on premalignant oral lesions and OSCC, dendritic cells pulsed with lysates of 4NQO-induced premalignant lesion cells were tested in vitro and in vivo for their capacity to stimulate T-cell reactivity to premalignant lesion cells and to OSCC. Spleen cells that were sensitized during coculture or in vivo with premalignant lesion-pulsed dendritic cells were cytolytic toward both premalignant lesion cells and OSCC, and secreted increased levels of interferon -gamma in response to challenge with premalignant lesion cells or OSCC as compared with spleen cells that were sensitized with keratinocyte-pulsed dendritic cells. Levels of CD8+ Tcells and interferon-gamma release were also increased in lesions of mice that were vaccinated with premalignant lesion-pulsed dendritic cells. The mice that were vaccinated against premalignant lesions were also more resistant to OSCC challenge. These studies show the feasibility of using premalignant oral lesions to stimulate immune reactivity against both premalignant oral lesions and     

circ_0004872 inhibits proliferation,invasion, and glycolysis of oral squamous cell carcinoma by sponged miR‐424‐5p

ObjectiveOral squamous cell carcinoma (OSCC) is one of the most common oral malignant tumors. circ_0004872 can inhibit the progression of gastric cancer, but its effect on the growth and metastasis of OSCC is still unclear.MethodsqRT‐PCR was used to detect the expression levels of circ_0004872 and miR‐424‐5p in cancer tissues of OSCC patients and adjacent normal tissues, OSCC cell lines, and human normal oral keratinocytes (HOK). CCK‐8, cell colony formation, flow cytometry, and transwell assay were used to detect cell proliferation rate, viability, apoptosis rate, and invasion ability. Use glucose/lactic acid kit to assay cell glycolysis ability. The dual‐luciferase reporter gene experiment and RIP experiment verified the relationship between circ_0004872 and miR‐424‐5p. The protein levels were examined by Western blot.ResultsThe expression of circ_0004872 was significantly downregulated in OSCC tissues and cells, and the overexpression of circ_0004872 inhibited the proliferation, vitality, invasion, and glycolysis of OSCC cells, and promoted apoptosis. The expression of miR‐424‐5p was greatly upregulated in OSCC tissues and OSCC cells. circ_0004872 can adsorb miR‐424‐5p in OSCC cells, and circ_0004872 can reverse the promoting effect of miR‐424‐5p overexpression on the process of OSCC cells.Conclusioncirc_0004872 suppresses the proliferation, invasion, and glycolysis of OSCC cells by sponged miR‐424‐5p, and promotes apoptosis, which can be used as a potential target for early diagnosis and targeted therapy of OSCC.     

血管内皮生长因子在口腔鳞状细胞癌中的表达及临床意义

摘 要 目的：在肿瘤成功生长、浸润和转移的过程中，血管形成是一个关键步骤。在肿瘤血管形成中，血管内皮生长因子(VEGF)是最强的血管生成因子这一观点已被普遍接受。本研究将调查口腔鳞状细胞癌(OSCC)中的VEGF的表达及临床意义。方法：通过免疫组织化学方法，评价了来自48例OSCC的经福尔马林固定、石蜡包埋预处理标本的VEGF表达水平，并且以阳性细胞百分比来表示标记指数(LI)。结果：OSCC内的VEGF表达显著强于邻近正常口腔粘膜。在仅接受手术治疗的病人中，在VEGF表达与淋巴结转移之间存在一个显著性关系(P＜0.05)。在所有病例，低LI均显著地与良好预后有关(P＜0.05)。结论：尽管关于VEGF的预后关联有争议，我们的结果仍建议这一因子适于评定口腔癌的预后。     

Proteomics-based identification of haptoglobin as a novel plasma biomarker in oral squamous cell carcinoma

BackgroundIdentification of tumor biomarkers to assist early diagnosis and monitoring of disease progression may potentially decrease the mortality and morbidity associated with oral cancer.MethodsA mouse model with oral squamous cell carcinoma (OSCC) induced by 4-nitroquinoline 1-oxide (4-NQO)/arecoline in drinking water was established to discover stage-associated biomarkers. A proteomics approach, immunoblot and immunohistochemical analysis were used to validate the expressed biomarkers in mice with OSCC. Human plasma samples were also collected and candidate biomarkers were evaluated using enzyme-linked immunosorbent assay.ResultsProteomic profiling of mouse plasma samples indicated that haptoglobin and apolipoprotein A1 precursor were up-regulated in the mice with OSCC. Immunoblotting of plasma samples and immunohistochemical analysis of oral tissues showed a significantly higher level of haptoglobin in the OSCC mice than in the control mice. The expression of haptoglobin in human plasma samples from 52 patients with OSCC indicated a strong correlation between the increasing levels of haptoglobin and the clinical stages of OSCC (P < 0.01).ConclusionsThese results suggest that haptoglobin has a great potential as a sensitive plasma biomarker for early detection of patients with OSCC.     

CCL7在口腔白斑及鳞癌中的表达

目的检测白斑、口腔鳞状细胞癌中趋化因子CCL7的表达情况。方法采用免疫组化技术检测18例白斑、35例口腔鳞状细胞癌及11例正常口腔黏膜中趋化因子CCL7的表达。应用SPSS软件对实验结果进行卡方检验。结果口腔白斑和口腔鳞癌病变组织中CCL7的表达率分别为33.3%（6/18）、77.1%（27/35）。结论 CCL7在异常增生型白斑及口腔鳞癌中的高表达,其表达水平与白斑恶变及鳞癌的侵袭与淋巴结转移有关。     

MTA-1基因在口腔疣状癌和鳞癌中的表达研究

【目的】研究肿瘤转移相关基因-1（metastasis—associatedgene1,MTA-1）在口腔疣状癌和鳞癌中的表达。【方法】取20例口腔鳞状细胞癌和10例口腔疣状癌组织及20例正常口腔黏膜组织标本,应用反转录多聚酶链式反应（RT-PCR）方法检测MTA-1基因mRNA的表达。【结果】口腔疣状癌组织与口腔鳞癌组织MTA-1基因mRNA的表达水平明显高于正常口腔黏膜上皮组织（P〈0．05）;口腔疣状癌中MTA-1基因mRNA的表达显著低于口腔鳞癌（P〈0．05）。【结论】MTA—1基因可能在口腔疣．状癌和口腔鳞状细胞癌的发生发展中起作用;可能与口腔鳞状细胞癌的浸润与转移密切相关。