硫化银染重金属离子的组织学方法在脑组织的应

改良的 Timm 硫化银方法能使大脑组织的重金属染色呈特异性分布.海马结构和纹状体染色最深,垂体和杏仁核染色次之。海马结构内的染色呈典型的带状模式,并和某些特定的神经终末成份分布相一致。本文讨论了硫化银染色方法用于研究在损伤、病变或某些操作后海马结构内特定神经成份可塑性变化的可能性.     

An efficient optimized method for isolation of villous trophoblast cells from human early pregnancy placenta suitable for functional and molecular studies

PROBLEM: The uniqueness of the human placenta cannot be replaced by animal models. In vitro studies are compulsory to elucidate the biology of human placenta and require isolation and purification of villous trophoblasts, which can be used in molecular and functional studies. Constant improvement in the isolation technique is required to obtain a high yield of pure trophoblast cells with high viability and well preserved morphology. METHOD OF STUDY: Optimized isolation procedure for human villous trophoblasts based on mild enzymatic treatment, Percoll gradient centrifugation and additional purification step involving positive or negative immunoselection on magnetic beads is described. RESULTS: A simple and effective isolation protocol gave a reasonably high yield of villous trophoblast cells with high purity and viability, and excellent morphology as assessed by flow cytometry and electron microscopy. CONCLUSION: This protocol provides an efficient, optimized method for isolation and enrichment of villous trophoblast cells, suitable for phenotypic, ultrastructural, molecular and functional analyses and for establishment of primary cultures.     

连续监测动物眼前后房压强差的实验方法探索

建立一种连续监测正常动物眼前后房压强差的实验方法.将高精度的CP300空气压差传感器改装成测量前后房液体压差传感器,通过穿刺法,将测量装置的正负极分别植入实验动物眼的后房和前房,连续测量动物前后房压强差,获得了数小时的有效实验数据,初步验证了该方法的可行性.