LIGHT AND ELECTRON MICROSCOPIC STUDY OF NONFUNCTIONING PARATHYROID CARCINOMA

A case of nonfunctioning parathyroid carcinoma in a 69-year-old female has been studied by light and electron microscopy. The tumor, located on the left side of the anterior neck, was well encapsulated by connective tissue but showed invasion to the capsule and to the thyroid. The tumor cells exhibited a trabecular arrangement surrounded by capillary networks but focally showed several ductal structures. They were polygonal in shape, had a large nucleus showing frequent mitosis and poor cytoplasm containing glycogen. Some tumor cells had clear and abundant cytoplasm, and resembled water-clear cells of the parathyroid. Immunohistochemically, no thyroglobulin was demonstrated in the tumor tissue. Electronmicroscopically, the tumor cells with high N/C ratio contained poorly developed cell organelles and abundant glycogen particles. They were poor in secretory granules and had no conglomeration of lipid. Desmosomes and tonoflbrils were observed. The ratio of the reported number of nonfunctioning parathyroid carcinoma to that of functioning one in Japan was compared with that in western countries. No difference of the ratio was found between these two, when identical criteria were employed.     

金黄地鼠甲状旁腺内神经纤维的电镜观察

应用电子显微镜观察了６０例金黄地鼠甲状旁腺内神经纤维分布情况，结果表明：各年龄组地鼠甲状旁腺血管周隙存在有髓和无髓神经纤维，以无髓纤维为多，在成年和老年鼠，一些无髓神经纤维直接与腺实质主细胞相邻。     

胎儿晶状体囊及晶状体上皮发育过程的光,电镜观察

应用手术显微镜、光、电镜观察了不同胎龄晶状体囊的血管、晶状体囊及晶状体上皮超微结构的发育.其结果表明:囊的前面的血管是来自虹膜小环.囊后面的血管是来自透明动脉.血管随胎龄增长而逐渐减少,直至第32周完全消失.晶状体上皮细胞超微结构的发育是:随胎龄增长上皮细胞由立方形→低立方形;核由长椭圆形→扁椭圆形.胞质中各种细胞器逐渐减少.但细丝逐渐增多,此提示晶状体的弹性与日俱增.而粗面内质网在胎龄第5～6个月时最为发达,这说明晶状体蛋白质的合成旺盛,以提供晶状体上皮向晶状体纤维过渡所需的物质基础.文内并就先天性和后天性白内障的病因进行了讨论.     

中华鳖十二指肠组织学的光镜和扫描电镜观察

目的：了解中华鳖十二指肠的组织学。方法：Ｃａｒｎｏｙ固定,石蜡包埋,切片,采用ＨＥ染色法,ＰＡＳ反应,ＲＮＡ反应,用光镜和扫描电镜对中华鳖十二指肠的组织学进行观察。结果：１．粘膜和粘膜下层向肠腔内凸起形成一个皱襞,使肠腔呈∩形;２．肠绒毛呈指状凸起,微绒毛非常发达,呈絮状;３．无十二指肠腺;４．在肠绒毛基部有似隐窝;５．粘膜上皮主要由二层组织组成,但有些区域仅有一层细胞;６;柱状细胞的纹状缘ＰＡＳ     

鼠心房起搏样细胞的光镜和电镜观察

目的探讨大鼠和小鼠心房传导组织以外的起搏样细胞。方法应用石蜡、冰冻及超薄切片,采用HE、Masson和胆碱脂酶组织化学染色,分别在光镜和电镜下观察大鼠和小鼠的心房组织,寻找心房组织中隐匿的起搏细胞样细胞。结果右心房肌层内散在、无规律地存在一些圆形或卵圆形细胞,这些细胞核相对较大,细胞质清亮,细胞器较少。它们具备窦房结内起搏(P)细胞的特征。结论大鼠和小鼠左、右心房组织内存在起搏细胞样细胞,它们很可能是心房异位搏动的形态学基础。     

狗股动脉弹性纤维的扫描电镜观察

用甲酸消化法制取正常股动脉弹性纤维标本，冷冻干燥后进行扫描电镜观察。见到内弹性膜上有许多散分布的窗孔，中膜的弹性经组织呈复杂的网状，外膜的弹性组织以粗大的纵向分层排列的弹性纤维为主。     

肉芽组织破纤维细胞溶酶体包裹相的电镜观察

本文对大白鼠新生肉芽组织内的破纤维细胞进行了超微结构和细胞化学的观察。电镜下可见破纤维细胞内含有大量的各级溶酶体,这些溶酶体的酸性磷酸酶反应均呈阳性。溶酶体不但能与异噬泡和自噬泡融合,还能主动吞噬细胞器和溶酶体等。溶酶体的主动吞噬有三种形式,即突起包裹、内陷包裹和混合包裹。突起包裹的膜由五层结构组成,即两层单位膜,两层亮层,及中央板。大溶酶沐还以出芽、纹窄的形式形成小溶酶体。溶酶体的这种活动可能与细胞吞噬机能的自我调节有关。本文还就破纤维细胞与巨噬细胞之间的关系进行讨论。     

A LIGHT AND ELECTRON MICROSCOPIC STUDY ON THE PANCREATIC DUCTAL SYSTEM

To Investigate morphological features of the human pancreatic ductal system, light and electron microscopic studies were performed, on the comparative basis with those of several species of mammals. The human pancreas possessed relatively well–developed ductal system, as compared with those of other mammals. Furthermore, it had unique centroacinr cells containing numerous mitochondria. The mode of innervation to the ducts, and cytological features such as cilia formation and mucin production were also revealed. Several kinds of endocrine cells including A, B, D₁, EC_n, presumptive EC₁, and presumptive EC₂ or K could be identified, but a few remained unclassiflable. The above findings are considered to be prerequisite for understanding the physiology, especially of bicarbonate production, and pathology, especially of oncogenesis of the exocrine and endocrine pancreas. ACTA PATHOL. JPN. 33: 297–321, 1983.     

免疫组化电镜观察血管加压素(Vp)在丘脑下部及神经垂体的分布

应用免疫组化电镜现察视上核、室旁核及神经垂体内Vp样物质的分布及定位。发现免疫反应阳性Vp物质主要出现在高尔基体扁平膜囊内、粗面内质网核糖体上、神经分泌粒、树突、轴突以及非对称性突触后膜内。神经垂体的无髓神经纤维及赫令氏体内显有Vp样颗粒。通过Vp样物质的分而和定位研究,本文并进行了有关Vp合成途径的探讨。     

大鼠孤束核内NT-LI成分生后发育和衰老变化的光镜和电镜观察

用免疫细胞化学和电镜相结合技术,在光镜和电镜水平对生后4天、15天、30天、90天、300天和760天6组大鼠孤束核内神经降压肽样免疫反应(NT-LI)成分的生后发育和衰老变化进行了定量研究。光镜下孤束核内NT-LI胞体和终末主要分布于最后区平面,多见于背侧亚核、内侧亚核和连合核内。电镜下内侧亚核内可见NT-LI胞体、树突、轴突及终末。6组大鼠孤束核内NT-LI细胞均数和内侧亚核内NT-LI终末密度及其突触密度均以生后4天至15天间增长最快,NT-LI细胞数在生后15天达到最高,NT-LI终末及突触密度在生后90天达到最高。三者在生后300天时均明显减少。发育期内侧亚核内NT-LI终末构成的突触以Gray Ⅰ型为主,至老年期则变为Gray Ⅱ型占优势。发育期内侧亚核内以含清亮囊泡伴颗粒囊泡的NT-LI终末为主,老年期此类终末明显减少。只含清亮囊泡的NT-LI终末从生后至老年变化不明显。     

兔窦房结的光镜观察

利用显微镜观察了20例成兔心窦房结的连续切片。窦房结位于腔耳角下方,界沟的静脉窦侧,心内外膜之间,长轴与界沟一致。窦房结上中部宽,下300变细。中心区位于中、上1/3交界处或中1/3处。构成窦房结的主要细胞为P细胞和T细胞。P细胞圆形或椭圆形,较小,核大圆,胞质淡染。T细胞形态介于P细胞和心肌细胞之间,位于结的上下端及周边。根据细胞的构筑把结分为P细胞区和T细胞区。结平均为6.25×2.23×0.26mm。     

LIGHT AND ELECTRON MICROSCOPIC OBSERVATIONS OF THE MYOCARDIUM OF DOGS IN HEMORRHAGIC SHOCK

Morphologic change of the myocardium of the dog were observed in oligemic and normovolemic shock produced by Wiggers' standard method.
Subendocardial hemorrhages, usually superficial and located mostly in the ventricular aspect of the septum, were remarkable in the normovolemic shock group, but rare in the oligemic shock group. The zonal lesion appeared in the early period of oligemic shock becoming more apparent with lapse of time. Ultrastructural studies revealed some differences of the findings of the zonal lesion between oligemic and normovolemic shock. In oligemic shock, the zonal lesion was identical to the supercontraction of myofilaments and dislocation of mitochondria. Supercontraction of myofibrils was usually unilateral, and herniation of damaged myocytes was frequently noticed in the unilateral lesion. In the normovolemic shock, supercontracted areas near the intercalated disc were usually stretched and the myofilaments became irregular in arrangement. In addition, the accumulation of glycogen granules and presence of clear flocculent areas were observed in the cytoplasm of some myocardial cells.
Cell edema, swelling and destruction of mitochondria and contraction bands were found in the damaged myocyte, especially in the terminal stage of normovolemic shock. Swelling of the capillary endothelial cell was already observed in the early period of oligemic shock. In addition, the pathogenesis of the zonal lesion and the mechanism of cardiac depression in hemorrhagic shock were discussed.     

人胎视网膜发生的光镜和电镜观察

本文在光镜下观察40例人胎视网膜的发生,在电镜下观察15例人胎视网膜视细胞、双极细胞、节细胞的发育。结果表明:胚胎第9周时神经上皮可分内、外成神经细胞层。第10周时内、外成神经细胞层之间的Chievitz带消失;第11周时节细胞从内成神经细胞层内迁;第13周节细胞与内成神经细胞之间出现内网层;第16周始双极细胞从外成神经细胞层中内迁形成外网层和内核层。第20周后视网膜各层形成。而视细胞、双极细胞、节细胞的超微结构于胎儿8个月后才发育完善。其结构与成人基本相同。     

COLISTIN NEPHROTOXICITY: REPORT OF A CASE WITH LIGHT AND ELECTRON MICROSCOPIC STUDIES1

A case of colistln nephrotoxicity without history of previous renal disease was studied by light and electron microscopy of a renal biopsy specimen obtained 11 days after discontinuation of the drug. The histologic picture showed variation from early acute necrosis to regeneration of the damaged renal epithelium. There appeared to be difference of susceptibility among individual tubular epithelial cells to sodium colistinmethanesulfonate in the development of nephrotoxicity. Morphological changes were essentially similar in both the proximal and distal tubules. Indentation of the nuclear membranes was conspicuous and appeared to be demonstrable earlier than the cytoplasmic alterations. ACTA PATH. JAP. 19: 55?67, 1969.     

CB-HRP法研究中两种显色反应和两种平板包埋法的光镜电镜比较观察

以CB—HRP为标记物,分别用硝普钠(SNP)和庚钼酸铵(AHM)作四甲基联苯胺(TMB)反应的稳定剂,再用改进的Aldes平板包埋法(玻璃纸平板包埋法)包埋切片,对树鼩颈髓前角运动神经元作了光镜和电镜的比较观察。发现在光镜水平,TMB—SNP和TMB—AHM这两种显色反应对显示神经元的胞体和近端树突无明显差别。但在显示神经元的远端树突,尤其是白质树突方面,TMB—SNP反应明显优于TMB—AHM反应。而在电镜下,以AHM为稳定剂的标本,神经元间的突触、神经微丝、线粒体等亚细胞结构清楚;以SNP为稳定剂者,这些亚细胞结构,甚至神经细胞都难以辨认。改进的Aldes平板包埋法的电镜效果与原Aldes平板包埋法一致或更好,而方法更简便。     

ENZYME HISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDY OF A VIRILIZING ADRENOCORTICAL ADENOMA

Enzyme histochemical and ultrastructural studies of a "dexamethasone-suppressed" virilizing adrenocortical adenoma and the attached cortex revealed that tumor cells showed little activities of some lysosomal enzymes and scarcity of lipofuscins and dense bodies of lysosomal type, forming a marked contrast to the cells of zona reticularis and the virilizing adenomas previously reported. The other findings of tumor cells, such as a pattern of activities of dehydrogenases including 3 β-hydroxy steroid dehydrogenase and the morphology of mitochondria, were those of reticularis cells. The findings showed that scantiness of lipofuscins did not rule out the possibility of adenoma producing adrenal androgen, dehydroepiandrosterone. Most of the tumor cells as well as reticularis cells were positive for alkaline phos-phatase, the activity of which was interpreted as the effect of ACTH stimulation.     

鸡蛋壳和壳膜的电子显微镜观察

本文对鸡正常蛋和软壳蛋的蛋壳和壳膜进行了电子显微镜观察,并比较了正常蛋和软壳蛋的组织结构,分析了软壳蛋的产生原因。     

HUMAN CYTOMEGALOVIRUS INFECTION: ELECTRON AND LIGHT MICROSCOPIC OBSERVATIONS OF THE PAROTID GLANDS OF AN AUTOPSY CASE

HCMV infection in the parotid gland removed at autopsy from a 5-month-old male infant with generalized cytomegalic inclusion disease was investigated electron- and light- microscopically, and virus invasion, synthesis and release in the host cell were described and compared with the results of in vivo experiments previously reported.
In the infected nucleus several types of inclusions were formed, and a virus-synthesizing matrix was seen to produce numerous developmental forms of the virus, usually with, but infrequently without, a variable core. Three unusual structures were seen in the nucleus of cytomegalic inclusion cells! tortuous strings, bamboo-like virus-synthesizing bodies and intranuclear sacs containing vesicles. The coat of mature HCMV in the cytoplasm appeared to be a triple-layered structure. The release of viruses into the parotid glandular lumen occurred by emiocytosis.     

人窦房结和房室结微血管的扫描电镜观察

本文用4例新鲜婴儿尸体,采用血管铸型扫描电镜法,观察人窦房结、房室结微血管的立体构筑。结果表明,窦房结的血管床是由微血管网构筑成一椭圆形网状结构,中央动脉贯穿于结的长轴,由此动脉向周围发出分支,逐级分为许多微动脉和毛细血管前微动脉,最后在结的浅部分成毛细血管网。毛细血管后微静脉的收集范围,则显示出按区收集的特点。而房室结微血管构型是一扁圆形的微血管网,结的浅部为一薄层比较细密的毛细血管网。透过网眼,可见有粗大的、彼此吻合的窦状静脉丛。房室结动脉由结一侧进入结内,向周围逐级分支,形成毛细血管前微动脉,穿行于静脉丛之间,在结的浅部连于毛细血管网。毛细血管后微静脉的始端有环形缩窄,为平滑肌压痕。     

ELECTRON MICROSCOPIC STUDY ON MITOCHONDRIA IN HÜRTHLE CELL ADENOMA OF THYROID

In this investigation Hürthle cell adenoma of the thyroid was observed by electron microscopy. The cytoplasm of the cells consisted chiefly of an accumulation of numerous irregularly shaped mitochondria. Very few other organellae were observed. In addition electron microscopy revealed various degrees of electron density in the matrix of the mitochondria. When 3 dimensional models of the mitochondria were constructed, they revealed the mitochondria to have 3 dimentional forms which sometimes looked as if they might be dividing into two or three parts. The authors felt that this indicated a process of vigorous division of mitochondria and an extension of their life span. However, it was not possible to identify the cause of this division of mitochondria.