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1.
Andrew P. Mariani 《Developmental dynamics》1987,179(1):25-39
The organization of the outer plexi-form layer (OPL) of the pigeon retina is described by electron microscopy and Golgi impregnation. Six types of photoreceptor, four types of horizontal cell, eight types of bipolar cell, and an interplexiform cell type were found by Golgi impregnation. The OPL was tri-stratified due to the endings of the photoreceptors at three different levels. This stratification was reflected in the laminar arrangement of the dendrites of the horizontal and bipolar cells. Electron microscopy showed that the synaptic endings of the photoreceptors made ribbon synapses, both triads and dyads, and basal junctions with the process of second-order neurons. Horizontal cells formed conventional chemical synapses, while horizontal cell axon terminals were extensively linked by gap junctions. 相似文献
2.
Mack AF 《Neuroscience》2007,144(3):1004-1014
In the retina of many lower vertebrates, the arrangement of cells, in particular of cone photoreceptors, is highly regular. The data presented in this report show that in the retina of a cichlid fish (Astatotilapia burtoni) the regular arrangement is not restricted to cone photoreceptors and their synaptic terminals but can be found in elements of the inner retina as well. A variety of immunocytochemical and other markers was used in combination with confocal microscopy on whole-mount preparations and tangential sections. Nearest neighbor analysis was performed and density recovery profiles as auto- and cross-correlograms were generated. Cells displaying a regular arrangement of their synaptic processes in matching radial register to each other were identified for each major retinal neuronal cell type except ganglion cells (i.e. photoreceptors, horizontal cells, bipolar cells, and amacrine cells). The precise location of some of the corresponding cell bodies was not as regular but still non-random, however there was no spatial cross-correlation between cell bodies of different types. The radial processes of Müller glial cells displayed a distribution correlating to the arrangement of photoreceptors and neurons. Thus, for one Müller glial cell I found two PKC-positive cone bipolar cells, a spatially corresponding grid of parvalbumin-positive amacrine cell processes, one H1 horizontal cell, and two pairs of double cones. There was no evidence among ganglion cells matching this pattern, possibly due to the lack of suitable markers. Although many other cell types do not follow this matching regular mosaic arrangement, a basic columnar building block can be postulated for the retina at least in cichlid fish. This suggests a functional radial unit from photoreceptors to the inner plexiform layer. 相似文献
3.
4.
Summary When cat visual cortex (area 17) is reacted with an antibody to vasoactive intestinal polypeptide (VIP) a variety of neuronal
types is labelled. Many of the labelled neurons are bipolar in form and are most common in layers II and III, although significant
numbers of bipolar neurons are also encountered in layer V. Multipolar cells are also labelled. These are most frequent in
layer IV and have a variety of shapes. In layer I, the labelled cells are of three varieties, i.e. horizontal bipolar cells,
horizontal bitufted cells and multipolar neurons, while in layer VI the few VIP-positive neurons are horizontal bipolar cells.
This suggests that all of the VIP-labelled neurons in cat area 17 are non-pyramidal in form, and this has been confirmed by
electron microscopy.
In these preparations, axon terminals are also labelled and under the light microscope it can be seen that these terminals
occur both within the neuropil and around the cell bodies of some neurons, particularly neurons in layers II and III. Electron
microscopy has shown that all of the labelled axon terminals form symmetric synapses and that those in the neuropil synapse
with the shafts of smooth dendrites. These axodendritic synapses account for about 90% of the synapses formed by the labelled
axon terminals. The remainder of the labelled axon terminals synapse with the cell bodies of pyramidal neurons.
Parallels are drawn between these results and those previously obtained by examining those neuronal elements labelled with
VIP antibodies in rat visual cortex. 相似文献
5.
Connexin 35/36 (Cx35/36) gap junction protein is expressed in various regions of the brain, including the retina. In this work, the expression of Cx35/36 in the outer retina of carp was studied by immunocytochemistry. By light microscopy, strong punctate Cx35/36-immunoreactivity was observed in the outer plexiform layer. Double labeling experiments on vertical retinal sections showed that Cx35/36 puncta were localized beneath cone pedicles, stained by recoverin, but not on them. In addition, few of the dendrites of rod-dominant ON type bipolar cells (rod-ON-BCs), stained by PKCα, were labelled with Cx35/36 in the retinal sections. In isolated cell preparations, Cx35/36 was clearly expressed on the dendrites of cone-dominant ON type bipolar cells (cone-ON-BCs), but the expression was much less on rod-ON-BCs. Moreover, Cx35/36 puncta were found in the dendrites of isolated horizontal cells (labelled by GAD 65/67) driven by cones, including H1 and H2 cells, but not in those of cells driven by rods (H4 cells). At the ultrastructural level, reaction product was found in H1 and H2 cell dendrites invaginating cone terminals, but not in H4 cell dendrites invaginating rod terminals. Moreover, dendrites of cone-ON-BCs, were also labebed. These results suggest that Cx35/36 could be specifically involved in modulation of the cone signal pathway in the outer retina of carp. 相似文献
6.
Rod photoreceptor-specific mutations cause ectopic synapses to form between cone photoreceptor terminals and rod bipolar cell dendrites in degenerating retinas of rhodopsin transgenic (P347L) pigs and retinal degeneration mice. Since the mutations occur in rod photoreceptor-specific genes in these two models, it is not known if ectopic synaptogenesis occurs specifically due to some rod photoreceptor cell-autonomous properties of a mutation or as a general consequence of photoreceptor degeneration. In the Royal College of Surgeons (RCS) rat, a mutation in the receptor tyrosine kinase gene, Mertk, causes failure of the retinal pigment epithelial (RPE) cells to phagocytose shed photoreceptor outer segments; subsequently, both rod and cone photoreceptors die. The non-phagocytic phenotype of the RCS rat is RPE cell-autonomous and the photoreceptors degenerate secondarily. Here we show that in 35-day-old RCS rats, where a majority of rod and cone photoreceptors remained, rod bipolar cell dendrites had abnormal (flat-contact type) synaptic contacts with rod and cone terminals.Demonstration of ectopic synapses in the RCS rat suggested that ectopic synaptogenesis could occur as a result of photoreceptor degeneration, even when the rods and cones were developmentally normal. This further supported the hypothesis that ectopic synaptogenesis may be a common step in the disease progression of different forms of retinal degeneration that include photoreceptor death as a feature, such as retinitis pigmentosa. 相似文献
7.
l-Glutamate, the photoreceptor neurotransmitter, depolarizes horizontal cells and OFF-bipolar cells by ionotropic receptors and hyperpolarizes ON-bipolar cells by metabotropic receptors. Despite extensive light microscopy on the distribution of glutamate receptors in zebrafish retina, there are little ultrastructural data. Given the importance of zebrafish in studies on the genetic manipulation of retinal development and function, precise data on the synaptic neurochemical organization of the zebrafish retina is needed. Immunohistochemical techniques were used to determine the ultrastructural localization of glutamate receptor subunits GluR2, GluR4, NMDA2B (NR2B) and mGluR1α in zebrafish outer plexiform layer (OPL). These antibodies were chosen because of an apparent conservation of localization of GluR2, GluR4 and mGluR1α in the vertebrate OPL, while there is some support for NMDA receptors in the OPL. GluR2-immunoreactivity (IR) was in all horizontal cell dendrites that invaginated cone pedicles and rod spherules. Three arrangements of dendrites contained GluR-IR in rod spherules: classical-type with GluR2-IR on lateral horizontal cell dendrites, a butterfly-shaped horizontal cell dendrite, and a goblet-shaped dendrite, likely of bipolar cell origin. GluR4-IR was restricted to dendrites of OFF-bipolar cells that innervated rod and cone terminals. NR2B-IR was restricted to a subtype of cone ON-bipolar cell. mGluR1α-IR was restricted to ON mixed rod/cone (Mb) bipolar cells whose dendrites innervated rod and cone synaptic terminals. The presence of mGluR1α on Mb bipolar cell dendrites is consistent with a role in retrograde endocannabinoid suppression. The subunit composition of glutamate receptors should affect the kinetics and pharmacology of these cells to glutamate receptor activation. 相似文献
8.
Summary The dopaminergic amacrine cells of the cat retina have been stained by immunocytochemistry using an antibody to tyrosine hydroxylase (Toh). The complete population of Toh+cells has been studied by light microscopy of retinal wholemounts to evaluate morphological details of dendritic structure and branching patterns. Selected Toh+amacrine cells have been studied by serial-section electron microscopy to analyse synaptic input and output relationships. The majority of Toh+amacrine cells occur in the amacrine cell layer of the retina and have their dendrites ramifying and forming the characteristic rings in stratum 1 of the inner plexiform layer. A minority of Toh+cells have cell bodies displaced to the ganglion cell layer but their dendrites also stratify in stratum 1. All Toh+cells have some dendritic branches running in stratum 2 as well as in stratum 1, and frequently they have long axon-like processes (500–1000 m long) dipping down to run in stratum 5 before passing up to rejoin the major dendritic arbors in stratum 1. In addition Toh+stained processes follow blood vessels in the inner plexiform layer and in the ganglion cell layer. A population of Toh+cells found in the inferior retina appears to give rise to stained processes that pass to the outer plexiform layer and therein to run for as far as one millimeter.Electron microscopy reveals that Toh+amacrine cells are postsynaptic to amacrine cells and a few bipolar cell terminals in stratum 1 of the inner plexiform layer and are primarily presynaptic to All amacrine cell bodies and lobular appendages, and to another type of amacrine cell body and amacrine dendrites hypothesized to be the A17 amacrine cell. The Toh+dendrites in stratum 2 are presynaptic to All lobular appendages primarily. Stained axon-like processes running in stratum 5 prove to be presynaptic to All amacrine dendrites as they approach the rod bipolar axon terminals and they may also be presynaptic to the rod bipolar terminal itself. The Toh+stained dendrites that have been followed in the outer plexiform layer run along the top of the B-type horizontal cell somata and may have small synapses upon them. The only clear synapses seen in the outer plexiform layer are from the Toh+profiles upon vesicle filled amacrine-like profiles that are in turn presynaptic to bipolar cell dendrites in the outer plexiform layer. We presume the cells postsynaptic to the Toh+dendrites in the outer plexiform layer are interplexiform cells. Finally the Toh+profiles that course along blood vessel walls and in the ganglion cell layer appear to end either against the basal lamina of the blood vessel or at intercellular channels of vesicle-laden Muller cell end-feet. 相似文献
9.
Terzibasi E Calamusa M Novelli E Domenici L Strettoi E Cellerino A 《Neurobiology of aging》2009,30(5):819-828
We have investigated morphological changes in second-order neurons of the mouse retina during aging by using immunohistochemistry and electron microscopy. We observed sprouting of rod bipolar cells dendrites and horizontal cells arborizations: neuronal processes of both neuronal types showed irregular extensions beyond the outer plexiform layer, toward the outer limiting membrane, as well as into the outer nuclear layer (ONL). These processes were first observed in animals of 12 months of age and increased in numbers steadily until 24 months, which represent the last age examined. The ectopic processes are decorated by puncta immunoreactive for pre-synaptic markers typical of photoreceptor terminals juxtaposed to post-synaptic neurotransmitter receptors, demonstrating the presence of the entire molecular machinery of functional synapses. Electron microscopy confirmed that ectopic processes receive synapses from photoreceptor terminals. We conclude that during the second year of life retinal rod bipolar and horizontal cells undergo sprouting and form ectopic synapses in the ONL. 相似文献
10.
We have used the P23H line 1 homozygous albino rat to study how progressive photoreceptor degeneration affects rod and cone relay pathways. We examined P23H retinas at different stages of degeneration by confocal microscopy of immunostained sections and electroretinogram (ERG) recordings. By 21 days of age in the P23H rat retina, there is already substantial loss of rods and reduction in rod bipolar dendrites along with reduction of metabotropic glutamate receptor 6 (mGluR6) and rod-associated bassoon staining. The cone pathway is relatively unaffected. By 150 days, when rods are absent from much of the retina, some rod bipolars remain and dendrites of rod and cone bipolar cells form synaptic complexes associated with cones and horizontal cell processes. These complexes include foci of mGluR6 and bassoon staining; they develop further by 270 days of age. Over the course of degeneration, beginning at 21 days, bipolar axon terminals atrophy and the inner retina undergoes further changes including a reduced and disorganized AII amacrine cell population and thinning of the inner plexiform layer. Electroretinogram (ERG) results at 23 days show reductions in a-wave amplitude, in rod and cone-associated b-waves (using a double flash paradigm) and in the amplitude of oscillatory potentials (OPs). By 38 days, rod scotopic a-wave responses and OPs are lost. B-wave amplitudes decline until 150 days, at which point they are purely cone-driven and remain stable up to 250 days. The results show that during the course of photoreceptor loss in the P23H rat, there are progressive degenerative changes, particularly in the rod relay pathway, and these are reflected in the changing ERG response patterns. Later reactive changes involving condensation of cone terminals and neurotransmitter receptors associated with rod and cone bipolar dendrites and with horizontal cell processes suggest that at this stage, there are likely to be complex changes in the relay of sensory information through the retina. 相似文献
11.
Summary A Golgi-stained flat diffuse cone bipolar cell from a vervet monkey's retina (Cercopithecus aethiops), contacting six cones, was serially sectioned for electron microscopy (EM) to determine the types of synapses it made with the cone pedicles. All the synapses were basal (flat) contacts. Their distribution and ultrastructural type were similar at each pedicle. Approximately half the synapses were definable as triad-associated and the rest were elsewhere on the cone pedicle base. Their ultrastructure is the same regardless of those positions. About 25 synapses were made with each cone. Thus this type (DB2 of Boycott & Wässle, 1991) of flat diffuse cone bipolar cell is in contact with six cones through about 150 synapses. At the eccentricity studied each cone pedicle probably makes 90–100 basal synapses with between three and four DB2 bipolar cells. This is between two and three times the number that are made with all the types of invaginating bipolar cells. A brief review of cone photoreceptor synapses with bipolar cells shows that, for those so far examined in the primate retina, the dichotomy into two types of bipolar cell invaginating (ribbon-related), with axons ending in the b-layer of the inner plexiform layer (IPL) (hence presumptive On-bipolars) and flat (basal synapses), with axons ending in the a-layer of the inner plexiform layer (hence presumptive Off-bipolars) is the rule. But other vertebrate retinae, including that of the cat, also have bipolar cells which vary from this pattern. 相似文献
12.
Horizontal cells form gap junctions with each other in mammalian retina, and lacZ reporter analyses have recently indicated that these cells express the Cx57 gene, which codes for the corresponding gap junctional protein. Using anti-connexin57 antibodies, we detected connexin57 protein in immunoblots of mouse retina, and found punctate immunolabeling of this connexin co-distributed with calbindin-positive horizontal cells in the retinal outer plexiform layer. Double immunofluorescence labeling was conducted to determine the spatial relationships of connexin36, connexin57, the gap junction-associated protein zonula occludens-1 and the photoreceptor ribbon synapse-associated protein bassoon in the outer plexiform layer. Connexin36 was substantially co-localized with zonula occludens-1 in the outer plexiform layer, and both of these proteins were frequently located in close spatial proximity to bassoon-positive ribbon synapses. Connexin57 was often found adjacent to, but not overlapping with, connexin36-positive and zonula occludens-1-positive puncta, and was also located adjacent to bassoon-positive ribbon synapses at rod spherules, and intermingled with such synapses at cone pedicles. These results suggest zonula occludens-1 interaction with connexin36 but not with Cx57 in the outer plexiform layer, and an absence of connexin57/connexin36 heterotypic gap junctional coupling in mouse retina. Further, an arrangement of synaptic contacts within rod spherules is suggested whereby gap junctions between horizontal cell terminals containing connexin57 occur in very close proximity to ribbon synapses formed by rod photoreceptors, as well as in close proximity to Cx36-containing gap junctions between rods and cones. 相似文献
13.
Summary The first appearance and early development of synapses between photoreceptors, bipolar cells and horizontal cells were studied in the retina of larvalXenopus, between stages 37/8 and 46. Synapse morphology was reconstructed from examination of serial sections. Additional data were obtained from E-PTA stained tissue. Photoreceptors initially contacted horizontal cell dendrites via ribbon synapses at the photoreceptor basal surface (stages 37/8-39). Later, horizontal cell dendrites penetrated the receptor as digitiform processes (stages 39–40) which subsequently expanded extensively within the invagination (stages 40–44). At stages 41–46, a photoreceptor ribbon generally was associated with two horizontal processes in a synaptic dyad complex; each ribbon participated in 1 or 2 such complexes. Bipolar cell dendrites made contact with receptors via superficial or invaginating basal-type junctions. Such junctions were observed first at stage 39, but increased greatly in number beginning at stage 42. Basal junctions were characterized by marked staining of the paramembranous receptor cell cytoplasm and a 9–13 nm wide cleft containing an electron-dense material. No association of bipolar cell dendrites with photoreceptor ribbons was noted.Junctional complexes presumed to be gap junctions first were observed at stage 39 between photoreceptor bases and basal processes emitted by neighbouring photoreceptors. 相似文献
14.
Substance P is the preferred ligand for the neurokinin 1 (NK1) receptor. In vertebrate retinas, substance P is expressed by amacrine, interplexiform and ganglion cells. Substance P influences the activity of amacrine and ganglion cells and it is reported to evoke dopamine release. We investigated NK1 receptor expression in the rabbit retina using affinity-purified NK1 receptor antibodies. NK1 receptors were expressed by two distinct populations of retinal neurons. One is a population of ON-type bipolar cells characterized by axonal arborizations that ramified in the inner plexiform layer near the ganglion cell layer. Double-label studies showed that NK1 receptor-expressing bipolar cells were distinct from rod bipolar cells and from other immunocytochemically identified types of cone bipolar cells. Their density was about 2250 cells/mm2 in the visual streak and 1115 cells/mm2 in ventral mid-periphery. They were distributed in a non-random pattern. In the outer plexiform layer, the dendrites of these bipolar cells converged into heavily immunostained clusters having a punctate appearance. The density of these clusters in mid-peripheral ventral regions (about 13000 clusters/mm2) was similar to the reported cone density [Famiglietti and Sharpe (1995) Vis. Neurosci. 12, 1151-1175], suggesting these dendrites contact all cone photoreceptors. The second NK1 receptor expressing cell population corresponds to the tyrosine hydroxylase-containing amacrine cell population. NK1 receptor immunostaining was localized to the cell body and processes, but not to the processes that form the 'rings' that are known to encircle somata of AII amacrine cells. These findings show that NK1 receptor immunoreactivity is localized to a population of ON-type cone bipolar cells and to dopaminergic amacrine cells, suggesting that substance P acting on NK1 receptors influences multiple retinal circuits in the rabbit retina. 相似文献
15.
Summary The photoreceptor synapses of three representative cells of the six types of diffuse bipolar cell of the rhesus macaque monkey's retina are described at 3.5–4.0 mm eccentricity. Bipolar cell DB3 was found to be postsynaptic to 11 cones at 155 basal synapses; about 70% of these were triad associated. Bipolar cell DB4 was postsynaptic to eight cones at 52 ribbon synapses; in addition it was found also to make an average of two or three basal (non-ribbon) synapses per cone (total 23). The DB5 bipolar cell type had 57 invaginating synapses with seven cones. It too had basal synapses, but only two with each of three cones. The diffuse invaginating bipolar cell described by Mariani (1981) is identified as a member of the DB5 category. Dendrites of cone bipolar cell types which have axons ending in the a-layer of the inner plexiform layer make only basal synapses with the cone pedicle. Those so far investigated are the flat midget bipolar cell and the DB2 and DB5 flat diffuse bipolar cells. All bipolar cells whose axons terminate in the b-layer of the inner plexiform layer are postsynaptic at the ribbon synapses of the cone pedicles. They now appear to fall into two groups. Those whose dendrites are exclusively postsynaptic at the ribbons; these are the blue cone and invaginating midget bipolar cells. And the diffuse bipolar cell DB4, that has both ribbon and basal synapses in a ratio of about 2.31. It is uncertain into which category cell DB5 should be placed; its basal synapses are so few the cell could be anomalous. It now seems that at least one primate bipolar cell type may be like those of other vertebrates in having, as defined ultrastructurally, two different kinds of synaptic connection with its cones. The results are discussed in the context of a brief review of the photoreceptor synapses of other mammalian bipolar cells. 相似文献
16.
Summary This study describes non-granule cells in the fascia dentata of rhesus monkeys and baboons. Their cell bodies are located in the molecular layer and at the hilar border of the granular layer. They are called basket cells since their axons give rise to collaterals that branch in the close vicinity of the parent cell body and form symmetric synapses with dendrites and cell bodies of granule cells. These neurons are further classified with regard to the shape and location of their cell bodies and the orientation of their dendrites. Basket cells in the molecular layer are mainly bipolar with dendrites oriented perpendicular to the granular layer. These dendrites are densely innervated by presynaptic boutons forming asymmetric synapses. We have rarely observed molecular layer basket cells with dendrites traversing the granular layer and invading the hilus. We thus conclude that these cells are mainly activated by extrinsic afferents terminating in the molecular layer. Basket cells at the hilar border display pyramidal, fusiform or multipolar cell bodies that give rise to apical dendrites traversing the molecular layer and basal dendrites invading the hilar region. Large boutons establish asymmetric synapses with identified basal dendrites of these neurons. The dendrites of all types of basket cell are smooth, i.e. they had few or no spines. Many of them display varicosities. Cell counts in Cresyl Violet-stained sections revealed a ratio of basket cells to granule cells of 1:500. Essentially, the types of basket cell in the monkey fascia dentata are similar to those described previously for the rat. This contrasts sharply to our recent findings for pyramidal neurons and granule cells of the monkey hippocampus which showed an increased complexity and variability when compared with rodents. These data do not support the hypothesis that only local circuit neurons evolve in phylogeny. 相似文献
17.
The inner plexiform layer in the retina of the cat: electron microscopic observations 总被引:5,自引:0,他引:5
Helga Kolb 《Journal of neurocytology》1979,8(3):295-329
Summary Neural connections of cells ramifying in the inner plexiform layer of the cat retina have been studied by serial section electron microscopy. Flat cone bipolars and invaginating cone bipolars segregate their axon terminals to different sublaminae of the IPL (sublaminaa and sublaminab, respectively) where they relate to different subtypes of the same class of ganglion cell (a andb types respectively).Rod bipolar axon terminals end solely in sublaminab and synapse with amacrine cells (AI and AII). AI provides reciprocal synapses to clusters of rod bipolar axon terminals. The AII amacrine provides rod input toa type ganglion cells by means of chemical synapses and tob type ganglion cells through gap junctions with invaginating cone bipolar terminals.Amacrine cells exist which interconnect rod and cone bipolars, but some amacrines appear to be related specifically to neurons branching in particular sublaminae. Both large- and small-bodied ganglion cells have amacrine-dominated input while the medium-bodied ganglion cells with small dendritic trees have cone bipolar-dominated input. 相似文献
18.
应用顺行溃变和HRP逆行追踪相结合的方法对猫内侧丘系与丘脑皮质投射神经元在丘脑腹后外侧核内的突触联系组合型式进行了研究。电损毁一侧后索核后将HRP注射于对侧皮质躯体感觉颈、躯干、四肢代表区,电镜下在注射区同侧的丘脑腹后外侧核内可见到下列七种突触形式;(1)溃变的内侧丘系轴突终末与HRP标记树突形成的轴-树突触,较多;(2)溃变的内侧丘系轴突终末与HRP标记的神经元体形成的轴-体突触较少;(3)溃变 相似文献
19.
Summary A post-embedding, electron microscopic immunocytochemistry technique, modified from existing protocols, was used to examine the labelling patterns of GABA immunoreactivity and glycine immunoreactivity in goldfish retina. Retinae were fixed in mixed aldehyde solution, dehydrated in ethanol, staineden bloc with uranyl acetate and phosphotungstic acid and embedded in LR White resin. Substances were localized in thin sections by floating grids first on a drop of primary antiserum and then on a colloidal gold-IgG conjugate. Finally, grids were exposed to osmium vapour. The localization of GABA immunoreactivity matched that of [3H]-GABA uptake or glutamate decarboxylase immunoreactivity as described previously. In the outer retina, GABA immunoreactivity was found in the cell bodies and axon terminals of H1 horizontal cells and their dendrites opposite cone photoreceptor terminals. Selected amacrine cell bodies were labelled, as were many processes, both synaptic and non-synaptic, throughout the inner plexiform layer, including most amacrine cell processes contacting the synaptic terminals of type Mb bipolar cells. Numerous amacrine cells, their processes in the inner and outer plexiform layers, and photoreceptor terminals contained glycine immunoreactivity in a distribution similar to that shown by [3H]-glycine uptake. Despite the absence of osmium in the primary or secondary fixative, our protocol results in excellent visibility of synaptic structures and detectability of the colloidal gold immunolabel. Also, it does not cause extraction of the HRP/DAB reaction product and is therefore suitable for double-label analysis of neurons labelled with horseradish peroxidase. 相似文献
20.
Barhoum R Martínez-Navarrete G Corrochano S Germain F Fernandez-Sanchez L de la Rosa EJ de la Villa P Cuenca N 《Neuroscience》2008,155(3):698-713
Mouse models of retinal degeneration are useful tools to study therapeutic approaches for patients affected by hereditary retinal dystrophies. We have studied degeneration in the rd10 mice both by immunocytochemistry and TUNEL-labeling of retinal cells, and through electrophysiological recordings. The cell degeneration in the retina of rd10 mice produced appreciable morphological changes in rod and cone cells by P20. Retinal cell death is clearly observed in the central retina and it peaked at P25 when there were 800 TUNEL-positive cells per mm(2). In the central retina, only one row of photoreceptors remained in the outer nuclear layer by P40 and there was a remarkable deterioration of bipolar cell dendrites postsynaptic to photoreceptors. The axon terminals of bipolar cells also underwent atrophy and the inner retina was subject to further changes, including a reduction and disorganization of AII amacrine cell population. Glutamate sensitivity was tested in rod bipolar cells with the single cell patch-clamp technique in slice preparations, although at P60 no significant differences were observed with age-matched controls. Thus, we conclude that rod and cone degeneration in the rd10 mouse model is followed by deterioration of their postsynaptic cells and the cells in the inner retina. However, the functional preservation of receptors for photoreceptor transmission in bipolar cells may open new therapeutic possibilities. 相似文献