小剂量氯胺酮显著改善人工流产术的麻醉效果

目的 观察小剂量氯胺酮复合丙泊酚及芬太尼在门诊人工流产术中的麻醉和术后镇痛效果以及并发症的发生情况.方法 选择781例行无痛人工流产的患者,分为3组:A组,264例,芬太尼1.0μg/kg+丙泊酚1.5 mg/kg;B组,276例,芬太尼1.0μg/kg+氯胺酮0.2 mg/kg+丙泊酚1.5 mg/kg;C组,241例,芬太尼1.0μg/kg+氯胺酮0.3 mg/kg+丙泊酚1.5 mg/kg.术中出现体动时,追加丙泊酚20～40 mg/次.观察平均动脉压(MAP)、心率(HR)、最低脉搏氧分压(SpO2)值、丙泊酚总消耗量、清醒情况、术中麻醉效果、术后6 h镇痛效果及术后并发症的发生情况.结果 3组间术前、术中、术后的MAP、HR以及术中最低SpO2值的差异均无统计学意义(P值均＞0.05),3组间丙泊酚总量的差异有统计学意义(P＜0.05),A组丙泊酚用量最多[(131.59±30.12)mg],B组最少[(89.13±29.72)mg].B组术后恢复良好率最高(93.48%),术后恶心(6.88%)、呕吐(2.17%)的发生率最低(P值均＜0.05);B、C组术后均未发生疼痛,而A组疼痛发生率也较低,仅为1.14%.结论 小剂量氯胺酮复合丙泊酚及芬太尼术中麻醉的效果良好,术后镇痛效果佳,恶心、呕吐发生率较小.     

小剂量艾司氯胺酮复合丙泊酚用于成人无痛胃肠镜检查的有效性和安全性

目的 探讨在成人无痛胃肠镜检查中小剂量艾司氯胺酮复合丙泊酚的有效性和安全性。方法 收集2021年9—12月本院行无痛胃肠镜检查的患者180例,采用随机号码表法将患者分为艾司氯胺酮复合丙泊酚麻醉组(E组)与丙泊酚单纯麻醉(P组),每组各90例。E组静脉注射艾司氯胺酮0.20 mg/kg, 30 s后给予丙泊酚1.5～2.5 mg/kg; P组静脉注射与E组所用艾司氯胺酮同等容量的生理盐水,30 s后给予丙泊酚1.5～2.5 mg/kg,两组根据胃肠检查情况术中丙泊酚继续诱导。记录患者一般情况,丙泊酚的用量以及苏醒时间。记录患者入室时(T₀)、诱导时(T₁)、内镜经过咽喉部(T₂)和乙状结肠部(T₃)、出室时(T₄)的MAP、HR、SPO₂。记录诱导结束(T₁)时的BIS评分。记录不良事件发生率以及相应的处理方法。记录术后梦境的发生情况。结果 E组丙泊酚累积用量显著低于P组(P<0.05),而E组苏醒时间与P组差异无统计学意义。与T...     

靶控输注丙泊酚复合芬太尼、利多卡因或氯胺酮全静脉麻醉

目的探讨靶控输注芬太尼、利多卡因或氯胺酮对丙泊酚静脉麻醉效能、血流动力学、麻醉恢复以及药物费用的影响。方法随机选择250例ASA Ⅰ-Ⅲ级择期手术患者靶控输注丙泊酚(P组,n=50)、丙泊酚复合芬太尼2μg/L(PF组,n=53)、利多卡因4 mg/L(PL组n=52)或氯胺酮0.4 mg/L(PK1组,n=47)、氯胺酮0.6 mg/L(PK2组,n=48)全静脉麻醉,PF组手术结束前30min停用芬太尼。观察麻醉期间HR,MAP变化以及术后苏醒情况,记录给药时间、用药量,计算各组患者药物费用。结果P组、PF组、PL组患者麻醉期间HR无明显改变, MAP在麻醉诱导时降低(P<0.05),气管插管、切皮时PF组恢复正常,P组、PL组升高,以P组明显。PKI组、PK2 组麻醉诱导、气管插管前后、切皮前后及术中HR,MAP无明显改变(P>0.05)。与单用丙泊酚组相比,PF组、PL 组、PK1组、PK2组麻醉诱导与维持丙泊酚用量分别较P组降低20%-30%(P<0.01),药物费用降低28.19%- 36.75%,以PK2组明显。各组患者麻醉恢复无明显差异。结论芬太尼、利多卡因和氯胺酮能明显增强丙泊酚麻醉效能,血流动力学稳定,降低丙泊酚用量和药物费用,以氯胺酮0.6 mg/L最为明显。     

艾司氯胺酮联合丙泊酚在无痛膀胱镜检查中的应用

〔目的〕观察艾司氯胺酮联合丙泊酚在无痛膀胱镜检查中的麻醉效果.〔方法〕选取行无痛膀胱镜检查男性患者100例,年龄22～65岁,体重53～79 kg,ASAⅠ～Ⅱ级,随机数字表法分为:丙泊酚联合舒芬太尼组(PS组)和丙泊酚联合艾司氯胺酮组(PK组),每组50例.PS组缓慢推注舒芬太尼0.1μg/kg;PK组缓慢推注艾司氯胺酮0.15 mg/kg.后给予丙泊酚1～2 mg/kg,记录两组患者各时间点心率、平均动脉压、脉搏血氧饱和度,观察检查过程中体动反应,并记录丙泊酚用量、镜检时间、苏醒时间,以及丙泊酚注射痛、呛咳、呼吸抑制(SpO2<90％)、低血压和恶心呕吐发生情况.〔结果〕各时间点两组患者间心率、平均动脉压、脉搏氧饱和度在各时间点差异无统计学意义(P>0.05);两组间患者间丙泊酚用量、苏醒时间相比,差异无统计学意义(P>0.05);两组间恶心呕吐、体动及注射痛发生率相比,差异无统计学意义(P>0.05);与PK组相比,PS组呛咳、呼吸抑制及低血压发生率明显升高,差异有统计学意义(P<0.05).〔结论〕艾司氯胺酮联合丙泊酚能够达到无痛膀胱镜检查的镇静镇痛效果,呼吸抑制、呛咳及低血压等不良反应少,值得临床推广.     

丙泊酚配伍瑞芬太尼、氯胺酮用于无痛胃肠镜检查的研究

目的探讨丙泊酚配伍小剂量瑞芬太尼或小剂量氯胺酮在无痛胃肠镜检查中的镇静、镇痛效果和安全性。方法 726例自愿行无痛胃镜(n=417)及结肠镜(n=309)检查的患者分别分为1、2、3组:1组患者单纯静脉经泵注射丙泊酚(200ml/h),2组患者先静脉注射瑞芬太尼10μg,然后注射丙泊酚,3组患者先静脉注射氯胺酮0.2mg/kg,然后用泵注射丙泊酚。所有患者丙泊酚注射至睫毛反射消失、全身肌肉松弛时,内镜医生开始内镜检查,此时的丙泊酚使用剂量记为初始有效剂量。检查中如有体动或吞咽反射则追加丙泊酚0.5mg/kg,所有检查超过5min则接用丙泊酚3μg/(kg.min)维持,其中结肠镜检查患者时间超过10min时追加瑞芬太尼5μg,不再追加氯胺酮。观察各组患者麻醉诱导前后血压、心率及麻黄碱、阿托品使用率,记录丙泊酚初始有效剂量和使用总量、意识恢复时间和恢复正常行走时间;同时记录丙泊酚注射痛、体动发生率、呼吸暂停、喉痉挛等不良反应发生率。结果所有患者顺利完成内镜检查,无喉痉挛发生,患者满意度100%。1、2组患者麻醉诱导后血压、心率显著下降,而3组血流动力学平稳。2、3组体动和呼吸暂停发生率显著低于1组。胃镜检查中,2组苏醒最快,1、2组正常行走时间没有显著差异,3组苏醒时间和正常行走时间均显著长于1组和2组。肠镜检查中,3组苏醒和正常行走时间无显著差异。结论丙泊酚配伍小剂量瑞芬太尼可以减少内镜检查时丙泊酚初始有效剂量及使用总量;减少注射痛、体动以及呼吸暂停的发生,同时缩短恢复时间。丙泊酚配伍小剂量氯胺酮可以提供稳定的呼吸、循环状态,同时减少丙泊酚初始有效剂量和使用总量;虽然延长胃镜检查者恢复时间,但并不延长肠镜检查者恢复时间。     

小剂量氯胺酮对丙泊酚无痛人工流产术后恢复的影响

目的 观察小剂量氯胺酮能否缓解无痛人工流产术后的疼痛及是否会增加术后不良反应的发生率.方法 400例拟行无痛人工流产术的患者,随机分为F1、F2、FK1和FK2共4组,每组100例.麻醉时,F1组静脉注射芬太尼1 μg/kg,F2组静脉注射芬太尼1.5 μg/kg,FK1组静脉注射芬太尼1 μg/kg +氯胺酮0.1 mg/kg,FK2组静脉注射芬太尼1 μg/kg+氯胺酮0.2 mg/kg,然后4组均静脉注射丙泊酚1.5 mg/kg.术中有体动时追加丙泊酚20 mg.记录4组患者术中丙泊酚用量,清醒即刻和术后6h疼痛数字分级评分(NRS评分)及术后6h内恶心、呕吐等不良反应的发生率.结果 4组间年龄、体重、体质指数、手术时间、术后平均留院时间的差异均无统计学意义(P值均>0.05),F2、FK1、FK2组的丙泊酚用量显著少于F1组(P值均<0.01),FK1、FK2组显著少于F2组(P值均<0.01),FK2组显著少于FK1组(P<0.01).清醒即刻,4组间疼痛NRS评分的差异无统计学意义(P>0.05);但术后6 h,F1组完全无痛(疼痛NRS评分为0分)率为73.2%,显著低于其余3组(P值分别<0.05,0.01),FK1、FK2组术后6h内恶心呕吐、呕吐的发生率均显著高于F1组(P值分别<0.05、0.01).结论 无痛人工流产麻醉诱导时辅以小剂量氯胺酮能显著缓解术后疼痛,但有增加术后恶心呕吐的风险,应注意预防.     

丙泊酚与咪哒唑仑复合氯胺酮用于小儿心导管术麻醉的比较

目的 比较丙泊酚与咪哒唑仑复合氯胺酮用于小儿心导管术麻醉的优缺点与安全性.方法 选择2～12岁先天性心脏病行心导管术患儿60例,随机分为两组,入室时静脉注射氯胺酮2 mg/kg行基础麻醉,P组采用丙泊酚6 mg/(kg·h)复合氯胺酮3 mg/(kg·h)维持,M组采用咪哒唑仑0.15 mg/(kg·h)复合氯胺酮3 mg/(kg·h)维持.结果 两组患儿手术时间差异无统计学意义,血流动力学不稳定状况、呼吸支持及体动发生率差异无统计学意义,P组清醒时间(15.9±5.4)min短于M组[(21.2±5.9)min],差异有统计学意义(P<0.05).结论 丙泊酚与咪哒唑仑复合氯胺酮均能安全有效地进行小儿心导管术的麻醉,而丙泊酚复合氯胺酮麻醉患儿苏醒更加迅速.     

异丙酚复合芬太尼与复合氯胺酮用于门诊人流术麻醉效果的比较

将57例拟行人流术的早孕孕妇随机分为:异丙酚 芬太尼(PF组,n=28)和异丙酚 氯胺酮组(PK组,n=29).观察:注射异丙酚量患者是否诉疼痛;异丙酚用量;术中患者有无肢动出现及患者的苏醒状况等项目.比较异丙酚复合小剂量芬太尼或小剂量氯胺酮用于门诊人流术的麻醉效果和患者恢复状况.结果:异丙酚用量和患者麻醉苏醒时间二组之间无明显差异;注射异丙酚时患者诉疼痛者PF组明显高于PK组患者;术出现肢动情况PF组明显高于PK组.提示异丙酚复合小剂量芬太尼或氯胺酮用于门诊人流术麻醉,均能取得满意的麻醉效果,患者的恢复也都迅速、安全,不过异丙酚复合小剂量氯胺酮可能更理想.     

不同小剂量氯胺酮复合丙泊酚用于人工流产手术的临床观察

目的 探讨不同小剂量氯胺酮复合丙泊酚在人工流产手术中的麻醉镇痛效果及其安全、有效性.方法 选择120例ASAⅠ~Ⅱ级,妊娠6~9周且自愿终止妊娠孕妇,随机分成4组.A组（对照组）静注生理盐水5 mL,1 min后静注丙泊酚2 mg/kg;B组静注氯胺酮0.2 mg/kg、丙泊酚1.5～2 mg/kg;C组静注氯胺酮0.3 mg/kg、丙泊酚1.5～2 mg/kg;D组静注氯胺酮0.4 mg/kg、丙泊酚1.5～2 mg/kg.观察术中各组SBP、DBP、HR、SpO2的变化及麻醉效果、术后镇痛效果,记录各组丙泊酚用药总量、清醒时间、离院时间及术后恶心、呕吐等并发症.结果 术中丙泊酚总用量A、B两组明显多于C、D组（P＜0.05）;术后清醒时间D组明显长于A组（P＜0.05）,离室时间4组差异无统计学意义.A、B两组丙泊酚诱导后2 min SBP、DBP、HR与诱导前有显著差异（P＜0.05）,C、D两组麻醉过程中心率、血压比较平稳.B、C、D 3组麻醉、镇痛效果明显优于A组,术后恶心反应也少于A组（P＜0.05）.结论 小剂量氯胺酮与丙泊酚合用于人工流产手术,麻醉镇痛效果好,并发症少,0.3 mg/kg氯胺酮与丙泊酚复合应用尤为安全、有效.     

复合氯胺酮麻醉对老年抑郁症患者电休克治疗的认知保护作用:一项随机对照研究

目的 探讨小剂量氯胺酮在老年抑郁症患者电休克治疗(electroconvulsive therapy,ECT)的有效性和安全性.方法 将157例老年抑郁症患者随机分为丙泊酚麻醉组(P组)和丙泊酚复合氯胺酮麻醉组(KP组).KP组每次ECT麻醉前给予小剂量氯胺酮0.3 mg/kg,P组给予等量生理盐水.采用简易智力状态检...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.