AFu与AFP联合检测对原发性肝癌的诊断研究

对原发性肝癌（ＰＨＣ）患者２１例，正常体检人员６４例，肝病患者２７例，非肝脏良性消化系统疾病患者１８例，非肝脏恶性肿瘤患者２０例，进行血清α－Ｌ－岩藻糖苷酶（α－Ｌ－ｆｕｃｏｓｉｄａｓｅ，ＡＦｕ）务血清甲胎蛋白（ＡＦＰ）的测定，结果显示：ＰＨＣ组的ＡＦｕ值（１９８．３６±５２．９４ｎＫａｔ／Ｌ）显著高于其它各组病例（Ｐ＜０．００１），ＡＦｕ对ＰＨＣ诊断的特异性、敏感性、阳性和阴性预测值分别为９６．９％、７６．１９％、８０％、９６．１５％；准确度为９４％；ＰＨＣ患者ＡＦｕ的阳性率为５３．３３％，ＡＦＰ和ＡＦＰ联合检测对ＰＨＣ诊断阳性率达９３．３３％。     

外周血和支气管肺泡灌洗液CA50测定在肺癌诊断与监测中的价值

目的 探讨血清、支气管肺泡灌洗液（ＢＡＬＦ）中糖链抗原５０（ＣＡ５０）测定在肺癌辅助诊断及监测中的价值。方法 采用免疫放射分析法（ＩＲＭＡ）测定７０例肺癌患者血清和３０例Ｉ￣Ⅱ期肺癌ＢＡＬＦ中ＣＡ５０水平。结果 肺癌组血清ＣＡ５０水平明显高于良性肺病组，并随肿瘤分期增高而升高（Ｐ〈０．０１）。以１７Ｕ／ｍｌ为界，诊断肺癌的敏感性为６８．６％，特异性为９０．６％。３０例肺癌ＢＡＬＦ中ＣＡ５０水平显著     

α-L-岩藻糖苷酶联合甲胎蛋白对45例原发性肝癌诊断的价值

对１２４例肝内外良恶性疾病患者联合检测血清ＡＦＵ（α－Ｌ－岩藻糖苷酶）及甲胎蛋白（ＡＦＰ）含量,探讨ＡＦＵ对原发性肝癌（ＰＨＣ）的诊断价值。结果：ＰＨＣ患者血清ＡＦＵ含量明显高于非肝癌组。ＡＦＵ及ＡＦＰ在原发性肝癌组的阳性率分别为８４％和７１％,两者敏感性差异有显著性（Ｐ＜０．０５）,特异性和诊断符合率差异无显著性（Ｐ＞０．０５）。在ＡＦＰ阴性ＰＨＣ患者中,ＡＦＵ阳性率为８４％,两者联检的总阳性率为９５％。认为ＡＦＵ可作为一种新的肝癌标志物用于临床,与ＡＦＰ互补能提高ＰＨＣ的诊断率。     

血清及支气管肺泡灌洗液中CA50,CEA,ACE检测对肺癌诊断的价值

检测１５例肺癌、３０例良性肺病患者的血清和支气管肺泡灌洗液（ＢＡＬＦ）中３项肿瘤标志物：糖链抗原５０（ＣＡ５０），癌胚抗原（ＣＥＡ），血管紧张素转换酶（ＡＣＥ）的含量。结果表明：①肺癌组血清和ＢＡＬＦ中的ＣＡ５０、ＣＥＡ均显著高于良性肺病组（Ｐ＜０００１）。肺癌组血清ＡＣＥ明显低于良性肺病组（Ｐ＜０００１）；而ＢＡＬＦ中的ＡＣＥ两组间无明显差异（Ｐ＞００５）。②两组患者ＢＡＬＦ中ＣＡ５０，ＣＥＡ显著高于血清测值（Ｐ＜０００１）。而ＡＣＥ显著低于血清测值（Ｐ＜０００１）。③肺癌组ＣＡ５０、ＣＥＡ的检测敏感性为８６７％。提示：血清和ＢＡＬＦ中ＣＡ５０及血清中ＡＣＥ有助于鉴别诊断     

血清α－L－岩藻糖苷酶测定在原发性肝癌中的诊断价值

测定了３６例原发性肝癌（ＰＨＣ）及６９例其他肝脏疾病患者的血清α－Ｌ－岩藻糖苷酶（ＡＦＵ）活性。结果表明ＰＨＣ患者血清ＡＦＵ明显高于正常对照组和其他肝病组，ＰＨＣ患者中ＡＦＵ阳性率为７５％，并明显高于肝硬化（２０％）与转移性肝癌（３３％）。本文证明血清ＡＦＵ可视为诊断ＰＨＣ的一个有用的指标，尤其适用于ＡＦＰ阴性的病例     

酶联法测定血清糖抗原19-9与血清甲胎蛋白在诊断原发性肝癌中的敏感性

使用酶联免疫法对原发性肝癌（肝癌组）和非消化道癌与非消化道疾病（对照组）患者进行血清糖抗原１９－９（ＣＡ１９－９）和血清甲胎蛋白（ＡＦＰ）检测，结果为：ＣＡ１９－９肝癌组检测２９例，阳性２６例，阳性率为８９．６５％；对照组检测４０例，阳性１２例，阳性率为３０．００％，两者有非常显著差异（χ２＝１８．２８，Ｐ＜０．０１）。ＡＦＰ肝癌组检测５１例，阳性３２例，阳性率为６２．７５％；对照组检测４０例，阳性７例，阳性率为１７．５０％，两者有非常显著差异（χ２＝１８．７４，Ｐ＜０．０１）。肝癌组ＣＡ１９－９和ＡＦＰ两项检测的阳性率（８９．６５％和６２．７５％）也有非常显著差异（χ２＝６．７１，Ｐ＜０．０１）；两项联合检测原发性肝癌的阳性率由单测ＡＦＰ６２．０７％提高到９３．１０％。可以认为，血清ＣＡ１９－９是检测原发性肝癌的一项重要指标，与ＡＦＰ联合测定可提高其阳性率。本文对ＣＡ１９－９和ＡＦＰ诊断原发性肝癌阳性率的有关问题进行了讨论。     

5'核苷酸磷酸二酯酶同工酶Ⅴ对肝癌的诊断价值

检测９０例原发性肝癌（ＰＨＣ）和８４例良性肝病患者血清５′核苷酸磷酸二酯酶同工酶Ⅴ（５′ＮＰＤａｓｅ－Ⅴ），结果显示：ＰＨＣ组血清５′ＮＰＤａｓｅ－Ⅴ阳性率显著高于良性肝病组（Ｐ＜０．００５），５′ＮＰＤａｓｅ－Ⅴ在ＡＦＰ阴性或低浓度升高ＰＨＣ中阳性率为５９．５％。提示：①血清５′ＮＰＤａｓｅ－Ⅴ对ＰＨＣ的诊断具有重要价值。敏感性、特异性和准确性分别为７７．８％、９１．７％和８４．５％。②５′ＮＰＤａｓｅ－Ⅴ有助于ＡＦＰ阴性或低浓度升高ＰＨＣ的诊断。     

血清α—L—岩藻糖苷酶测定对原发性肝癌的诊断价值

测定了５８例原发性肝癌（ＰＨＣ）及６８例其他肝脏疾病患者的血清α－Ｌ－岩藻糖苷酶（ＡＦＵ）活性。结果，ＰＨＣ患者血清的ＡＦＵ活力明显高于正常对照组和其他肝病组，ＰＨＣ患者中ＡＦＵ阳性率为７４．１４％，并明显高于肝硬化组（１８．９２％）与急性肝炎组（２５．８１％）。结果证明，血清ＡＦＵ可为诊断ＰＨＣ的一个有用的指标，尤其适用于ＡＦＰ阴性的病例。     

肝癌标志物多项联合检测的临床意义

目的：评价肝癌标志物多项联合检测对原发性肝癌（ＰＨＣ）的诊断价值。方法：选择ＰＨＣ４１例、良笥肝病１０３例及４５名健康人进行血清α－Ｌ－岩藻糖苷酶（ＡＦＵ）和低密度脂蛋白与极低密度脂蛋白结合的γ－谷氨酰转肽酸疼 （ＬＤＬ－ＶＬＤＬ－ＧＧＴ）测定,并与甲胎蛋白（ＡＦＰ）联合检测。结果：ＡＦＰ、ＡＦＵ、ＬＤＬ－ＶＬＤＬ－ＧＧＴ对ＰＨＣＲ 敏感性分别是７３．２％、７８．０％、４３．９％,特异性分别是８９     

AFP、SF、AIF联合检测对原发性肝癌诊断的价值

用放射免疫分析法（ＲＩＡ）对４６例原发性肝癌（ＰＨＣ）患者及１４５例正常人进行血清甲胎蛋白（ＡＦＰ）、铁蛋白（ＳＦ）、酸性铁蛋白（ＡＩＦ）测定，结果ＰＨＣ组血清ＡＦＰ、ＳＦ、ＡＩＦ测定值显著高于对照组及其他肝病组（Ｐ＜０．００１），肝癌组ＡＦＰ阳性中为７８．２６％，联合测定ＳＦ、ＡＩＦＩａ性检出中可达１００％，提示血清八ＦＰ、ＳＦ、ＡＩＦ测定在ＰＨＣ诊断中有重要的价值，尤其联合测定ＡＦＰ，ＳＦ、ＡＩＦ３项可提高原发性肝癌的早期诊断率，降低了漏诊率。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.