氯化汞与大鼠脑突触体膜的相互作用

应用荧光探剂ANS,研究了氯化汞与大鼠脑突触体膜的相互作用。结果发现,氯化汞增强膜ANS复合物荧光强度。双倒数及Scatchard作图分析表明,其使膜-ANS复合物荧光强度增强的原因并非ANS荧光量子产率的改变,而是增加了突触体膜对ANS的结合容量和亲和力,亦即降低了膜表面负电荷密度。这一效应与其对膜流动性的影响是一致的。     

胺甲硫磷对大鼠血及脑乙酰胆碱酯酶的抑制作用

实验研究了胺甲硫磷在急性、亚慢性染毒时对大鼠血、脑乙酰胆碱酯酶（AchE）的影响，及在体外条件下，以人红细胞膜制备物为对象，脸甲硫磷抑制AchE的机理。结果表明，胺甲硫磷不仅在急性染毒时（1／10LD(50)）对血、脑AchE有抑制作用，而且当亚慢性（90d）染毒时，高剂量组（在1／25LD_(50)剂量下）对血、脑AchE也有抑制作用。体外试验表明，胺甲硫磷对人红细胞膜AchE的抑制作用具有剂量效应和时间效应关系，对AchE的抑制作用为不可逆性，其双分子速率常数较小，符合硫酮式有机磷化合物作用规律。     

大鼠肝微粒体膜脂流动性和膜表面负电荷密度的测定

本文利用荧光探剂ANS研究了大鼠肝微粒体膜脂流动性和表面负电荷密度的测定方法。所得结果表明:(1)在测定ANS荧光强度和荧光偏振度时,选用的最佳实验条件是:膜蛋白浓度为50μg/ml;在37℃下温育;膜与ANS温育15分钟;ANS在0～4℃下保存两周之内仍可考虑使用。(2)测定膜表面负电荷密度时,ANS滴定的浓度范围为6.25～100μmol/L。(3)测定ANS在膜中的相对荧光量子产率时,选用的膜蛋白浓度范围为12.5～200μg/ml。此外,实验还发现,向肝微粒体膜悬液中加入Cd~(2+)后能明显增加膜与ANS的结合,并能降低膜脂流动性和膜表面负电荷密度。     

胆红素自由基对人红细胞膜流动性的影响

用荧光偏振法研究胆红素自由基对人红细胞膜流动性的影响。结果表明，胆红素自由基使红细胞膜荧光偏振度（Ｐ）减少，微粘度（μ）降低，膜流动性增加。并讨论了上述现象产生的原因。     

马齿苋对氧自由基引发人红细胞膜损伤的保护作用

目的　探讨马齿苋提取物对人红细胞膜氧化损伤的保护作用。方法　采用邻苯三酚自氧化产生的超氧阴离子自由基 (O· -2 )致红细胞膜损伤 ,以此为膜过氧化损伤实验模型 ,以脂质过氧化降解产物丙二醛 (MDA)、荧光偏振度和膜脂流动性 (MFU)为指标 ,观察马齿苋提取物对人红细胞膜氧化损伤的保护作用。结果　红细胞膜丙二醛 (MDA)含量明显降低 ,荧光偏振度P值明显降低 ,膜质流动性 (MFU)明显增大 ,其作用随剂量增加而增强。结论　马齿苋提取物对氧自由基引发的红细胞膜损伤具有一定的保护作用     

测定红细胞膜脂流动性的荧光偏振法和激光喇曼光谱法的比较研究

红细胞膜脂流动性的研究日益受到人们的重视。本文以老化红细胞和年轻红细胞为实验对象,对测定膜脂流动性的荧光偏振法和激光喇曼光谱法进行了分析比较。指出:测定红细胞膜脂流动性以荧光偏振法较为理想。     

红景天甙元对酪醇对红细胞膜流动性影响的研究

目的：研究红景天甙元对醋酥（以下简称为甙元对酪醇）对红细胞膜流动性的影响。方法：以ANS（1—苯胺基－8－萘磺酸）和DPH（1,6－二苯基－1,3,5－已三烯）为探针,利用荧光分光光度计测定人红细胞膜的流动性。结果：甙元对醋醇对膜脂疏水区流动性无显著影响;甙元对酪醇随浓度的增加对膜脂极性区流动性有增强作用。结论：甙元对酪醇没有直接作用在膜脂流水区;甙元对酪醇作用在萨脂极性头部。     

葡萄糖－6－磷酸脱氢酶缺乏时红细胞膜氧化性变化

用荧光分光法测定红细胞膜过氧化脂质（ＭＤＡ），同时用荧光偏振法，以疏水性荧光探剂１，６－二苯基－１，３．５－己三烯（ＤＰＨ）标记正常人及葡萄糖－６－磷酸脱氢酶（Ｇ６ＰＤ）缺乏者的红细胞膜脂区，测定０℃和９０℃的偏振荧光，膜流动性以荧光偏振度表示。结果发现：Ｇ６ＰＤ缺乏时红细胞膜ＭＤＡ含量（１．４８±０．０９５）及荧光偏振度（０．２６３３±０．００４３）明显高于正常（０．３８２±０．０７２及Ｏ２２６１±０．００６９），Ｐ均＜０．０１．即红细胞膜有序程度增高，流动性降低结果提示，Ｇ６ＰＤ缺乏时红细胞膜发生了氧化性损伤，红细胞膜ＭＤＡ含量增加，膜流动性下降，膜ＭＤＡ含量与流动性下降相关．这可能是引起溶血的一个重要原因。     

胆红素自由基对人红细胞膜流动性的影响

用荧光偏振法研究胆红素自由基对人红细胞膜流动性的影响。结果表明,胆红素自由基使红细胞膜荧光偏振度(P)减少,微粘度(η)降低,膜流动性增加。并讨论了上述现象产生的原因。     

枸杞多糖对细胞膜流动性及蛋白激酶C的体外效应

研究枸杞多糖体外免疫调节效应的作用机制。以ＤＰＨ作为荧光探剂，采用荧光偏振法观察ＬＢＰ对兔红细胞膜流动性的影响。并以＾３２Ｐ－组蛋白内参入的放射性强度检测法，观察ＬＢＰ对淋巴细胞胞膜和胞浆蛋白激酶活性的影响。结果：１００ｍｇ／Ｌ ＬＢＰ可明显促进兔红细胞细胞膜的流动性，并能增强１０ｍｇ／Ｌ和２５ｍｇ／ＬＣｏｎＡ对膜流动性的促进作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.