原发性宫颈腺癌的临床病理及免疫组织化学分析

目的 探讨宫颈腺癌的病理分类,免疫组化表达特征,鉴别诊断及预后的因素。方法 对９８例原发性宫颈腺癌进行临床病理及免疫组化分析,并与１８例子宫内膜腺癌的免疫组化进行比较。其中５４例进行了跟踪随访。结果 将本组原发性宫颈腺癌分为５类,其中以宫颈内膜型（ｅｎｄｏｃｅｒｖｉｃａｌ）最为常见。免疫组化;癌胚抗原（ＣＥＡ）,人乳头瘤病毒１６及６Ｂ／１１型（ＨＰＶ１６及ＨＰＶ６Ｂ／１１）染色的阳性率分别为８３．     

食管腺癌病理组织学,组织化学和免疫组化观察

汕头地区１９７８－１９８８年病理确诊的２４８７例食管癌中，有食管腺癌４８例，占１．９３％，４８例食管腺癌的组织学可分６亚型：高分化普通型腺癌（１０例），低分化普通型腺癌（１１例），腺鳞癌（１８例）。粘液表皮样癌（４例），圆柱瘤样癌（１例），基底细胞样癌（４例）。各型的组化，免疫组化表达及预后均各有特点，提示食管腺癌组织学再分型有意义。     

卵巢转移性肺腺癌5例临床病理分析并文献复习

目的 探讨肺腺癌卵巢转移的临床病理特征及分子遗传学特点.方法 收集5例肺腺癌卵巢转移患者的临床资料,行HE、免疫组化EnVision两步法染色,对转移灶行二代测序检测,并复习相关文献.结果 5例患者平均年龄55.6岁,3例有肺腺癌病史,分别于肺癌术后45、47、57个月发生卵巢转移;2例以盆腔巨大包块就诊.5例患者均行...     

宫颈腺癌前驱病变的组织学及免疫组织化学观察

目的：探讨宫颈腺癌前驱病变的诊断和鉴别诊断。方法：对101例病理诊断为宫颈腺癌前驱病变－宫颈腺上皮内肿瘤的标本进行病理形态学观察,同时采用免疫组织化学SP法进行癌胚抗原（CEA）、增殖细胞核抗原（PCNA）、雌激素受体（ER）及人乳头瘤病毒（HPV6／11）的检测,结果：病理形态学分级标准根据核染色质的增多、核的异型性及细胞内粘液的数量将宫腺上皮内肿瘤分为3级,宫颈原位腺癌纳入Ⅲ级宫颈腺上皮内肿瘤中,其中46．53％（47／101）的病例合并鳞状上皮内病变或宫颈原位癌。免疫组织化学检查结果：CEA阳性率为44．55％（45／101）,此外37．62％（38／101）阳性部位表浅、仅局限于细胞的顶端及腔缘;PCNA强阳性率为63．54％（61／96）,在Ⅲ级宫颈腺上皮内肿瘤中全部呈强阳性表达;ER阳性率为81．48％（66／81）,但在部分阳性病例中ER不是均匀分布,仅局限阳性;HPV6／11在Ⅰ－Ⅲ级宫颈腺上皮内肿瘤中的检出率分别为34．09％（30／88）、31．82％（28／88）和3．41％（3／88）。结论：宫颈腺上皮内肿瘤的病理组织学分级具有重要的临床意义,CEA、PCNA及ER等免疫组织化学表达在诊断及鉴别诊断中具有一定的参考价值。     

15例肾乳头状囊腺癌临床病理分析

报道肾乳头腺癌１５例，大体均为多囊性，组织学可分为乳头状囊腺癌，嗜细胞乳头状囊腺癌和粘液性乳头状囊腺癌三型。免疫组织化学染色，瘤细胞呈高分子量角蛋白阳性（９／９例）ＥＭＡ呈腺腔面细胞膜阳性，支持该类种瘤起源于集合管。瘤体积多较大（平均直径９．６ｃｍ），８０％有肾周组织扩展，显示侵袭性临床行为，该瘤主要及肾髓质，侵儿集合管系统，与肾细胞癌比较，有相对不同的临床表现。     

卵巢克鲁根勃瘤与卵巢腺癌临床病理及性激素受体分析

卵巢克鲁根勃瘤（简称克氏瘤）最初被认为是上皮性肿瘤，且多数为胃肠道转移瘤。自此很多人把克氏瘤当作卵巢转移瘤的同义词。近来大多数学者认为克氏瘤应属胃肠道转移癌，并具有Ｋｒｕｋｅｎｂｅｒｇ描述的形态特点〔１〕。克氏瘤具有特殊的组织学形态及生物学行为，具有...     

女性生殖系统中肾管腺癌和中肾样腺癌的临床病理特征

目的 探讨女性生殖系统中的中肾管腺癌(mesonephric adenocarcinomas, MA)和中肾样腺癌(mesonephric-like adenocarcinomas, MLA)临床病理特征、诊断与鉴别诊断。方法 收集2例MA和3例MLA临床资料,行免疫组化EnVision两步法染色。采用一代基因测序检测2例MA中KRAS基因突变,运用NGS检测对2例MLA进行高通量测序,分析其临床病理特征并复习相关文献。结果 子宫颈MA与卵巢、子宫体的MLA形态学相似,呈腺管状、乳头状、筛状、实性结构、裂隙样和性索样结构,局灶小管腔内见均质嗜酸性物质。免疫表型：ER通常阴性或小灶阳性,PR阴性,5例均有TTF-1和GATA3反向染色现象。3例有KRAS基因突变,MLA可见CTNNB1、PTEN等基因突变。结论 MA和MLA有相似的形态学特征、免疫表型、基因突变和临床侵袭性,发病部位和周围有无中肾管残件/中肾管增生是鉴别的重要特征。     

卵巢表面上皮—间质肿瘤的病理组织学

本文重点讨论卵巢上皮性肿瘤的诊断标准及有关新分类的一些问题。1 卵巢表面上皮—间质肿瘤的—般特点卵巢表面上皮—间质肿瘤是WHO卵巢肿瘤组织学分类第2版中对“普通”上皮肿瘤的正式命名。它实际上包括了卵巢表面上皮伴有或不伴有间质成分的肿瘤。这群肿瘤不仅常见,而且组织学类型繁多。表面上皮肿瘤     

胃肝样腺癌的组织病理学和免疫组织化学研究

胃肝样腺癌的组织病理学和免疫组织化学研究李祥周张佃乾乐美兆胃肝样腺癌的组织病理学和免疫组织化学研究@李祥周@张佃乾@乐美兆...     

Histochemical Analyses of Tissue-Engineered Human Menisci

The field of tissue engineering remains one of the least explored areas of current meniscal research but holds great promise. In this investigation, meniscal fibrochondrocytes were isolated from fresh human meniscal tissue and seeded onto synthetic polyglycolic acid (PGA) scaffolds. Constructs were implanted into the dorsal subcutaneous space of athymic nude mice. Control scaffolds, devoid of meniscal cells, were simultaneously implanted in additional mice. Constructs were harvested over 12 weeks and treated with a variety of histochemical stains to analyze general specimen morphology, cellular viability and proliferation, and collagen secretion. Results indicate that meniscal fibrochondrocyte proliferation increased over the time of implantation with cellular consolidation occurring as the PGA scaffolding was progressively hydrolyzed. Collagen production also increased over time. There were favorable similarities between constructs and human meniscal controls in terms of cellular morphology, phenotypic expression, and collagen production. These initial findings demonstrate procedures supporting proliferation of meniscal fibrochondrocytes, expression of fibrochondral phenotype, and the formation of putative meniscal tissue.     

子宫颈腺癌与子宫内膜腺癌的组化及免疫组化观察

目的：探讨组化染色及免疫组化在宫颈腺癌与宫内膜腺癌诊断价值及临床意义。方法：采用组化染色及免疫组化Ｓ－Ｐ方法对宫颈腺癌及宫内膜腺癌的粘蛋白含量、分布及免疫组化阳性物的表达进行观察。结果：宫颈腺癌以含丰富的唾液酸粘蛋白为主，而子宫内膜腺癌以硫酸粘蛋白为主。波形蛋白（ｖｉｍｅｎｔｉｎ）在宫内膜腺癌阳性表达率６８．９％，而宫颈腺癌大多呈阴性表达，雌激素受体（ＥＲ）表达在宫内膜腺癌达６０．９％，而宫颈腺癌仅１９．６％，两者均差异有显著性（Ｐ＜０．０５）。而抑癌基因ｐ５３和癌基因ｃ－ｅｒｂＢ－２阳性率与癌肿的分级和预后有关。结果：粘蛋白组化染色及ｖｉｍｅｎｔｉｎ可帮助鉴别诊断宫颈腺癌及宫内膜腺癌，而ＥＲ、ｐ５３、ｃ－ｅｒｂＢ－２对病人的预后有一定意义     

Histopathological approach to prostatic contour alterations with the concept of left-right asymmetry

It has been suggested that prostate cancer spread has predictable patterns, with prostate cancers known to affect the prostatic contours. However, few systematic investigations have documented the associated contour alterations, especially at a clinically localized stage. The purpose of the present paper was to objectively evaluate prostatic contour alterations based on left-right asymmetry, and analyze the histopathological features and prognostic impact. One hundred and sixty-two asymmetrical contours with left-right asymmetry in the length >or=10%, and 278 cancer foci were observed in 114 radical prostatectomy specimens. Of the asymmetrical contours, 55 (34%) were caused by cancer, and of the cancer foci, 55 (20%) generated asymmetrical contours. Cancer-associated asymmetries showed significant correlations with aggressive behaviors such as cancer volume, Gleason score, positive surgical margin, and extraprostatic extension, although these were not significantly associated with unfavorable prognosis. The authors present basic data relating to altered prostatic contours, such as spatial distribution and causative pathological conditions. Clinicopathological characteristics concerning cancer-associated contour alterations, are also presented in detail.     

Sinonasal tubulopapillary low-grade adenocarcinoma. Histopathological,immunohistochemical and ultrastructural features of poorly recognised entity

We present the clinicopathological, histological and immunohistochemical findings of six cases of primary tubulopapillary low-grade adenocarcinoma of the sinonasal tract with ultrastructural examination in one case. Due to its unique features, we believe that primary tubulopapillary low-grade adenocarcinoma of the sinonasal tract represents a tumour entity different from any tumours generally recognised in the sinonasal region. Our cases had an equal sex incidence, with an age range of 44–76 years. The tumour has a tendency to recur, but none of our six patients developed metastases. We feel that it is important to separate this tumour entity from other types of sinonasal adenocarcinomas that exhibit a papillary growth pattern, as they frequently pursue a much more aggressive clinical course than the tumours in this study.     

卵巢良性和恶性粘液性囊腺瘤酸性粘蛋白的组化改变

用4种酸性粘蛋白组织化学染色法,观察90例卵巢良性和恶性粘渡性囊腺瘤酸性粘蛋白的含量和成分。发现粘液性囊腺癌酸性粘蛋白含量明显高于交界性粘液性囊腺瘤和钻液性囊腺瘤。绝大多数粘液性囊腺瘤酸性粘蛋白的主要成分是唾液酸粘蛋白,或唾液酸和硫酸两种粘蛋白的混合,仅少数瘤含有较多硫酸粘蛋白。与囊腺癌上皮比较,良性囊腺瘤上皮中唾液酸粘蛋白含量增多,或两种酸性粘蛋白均减少。据认为:①当囊腺瘤恶变时,酸性粘蛋白逐渐增加;②唾液酸粘蛋白(部分肿瘤含硫酸粘蛋白)增多是卵巢粘液性囊腺癌的组化特点;③酸性粘蛋白含量可能与肿瘤生物学行为有关。     

Clinicopathologic and Immunohistochemical Characteristics of Goblet Cell Type Adenocarcinoma of the Lung

Twenty-two resected goblet cell type adenocarcinomas of the lung were examined clinicopathologically and immunohistochemically. The stage and survival curve of goblet cell type adenocarcinomas were compared with those of 44 cases of pure or mixed Clara cell and bronchial surface epithelial cell (Clara and BSE) type adenocarcinomas. Each case of goblet cell type was matched with two cases of Clara and BSE type as to sex, age and date of surgery. In goblet cell type adenocarcinomas, lymph node metastasis was less frequently and intrapulmonary metastasis was more frequently detected than in other types of adenocarcinomas (p < 0.001 and p < 0.05, respectively). Goblet cell type adenocarcinomas showed better prognoses than Clara and BSE type adenocarcinomas. However, the estimated survival curves of those two groups become similar after adjustment of the TNM condition using Cox's proportional-hazard general linear model. This result indicated that the longer survival of goblet cell type adenocarcinoma was due to the characteristic distribution of TNM conditions, that is, unique local growth and low incidence of lymph node metastasis. When goblet cell type adenocarcinoma was macroscopically classified into two types, i.e. solitary peripheral nodule type (nodular type) and multifocal nodular type or consolidation of all or part of a lobe (diffuse type), the nodular type had better prognosis than the diffuse type (p < 0.05). Immunohistochemically, 83%, 11%, and 0% of goblet cell type adenocarcinomas were positive for NCC-CO-450, carcinoembryonic antigen (CEA), and surfactant apoprotein, respectively. Most Clara and BSE type adenocarcinomas were negative for NCC-CO-450, but positive for CEA and surfactant apoprotein. NCC-CO-450 was considered to be a good immunohistochemical marker of goblet cell type adenocarcinoma of the lung. These results indicated that goblet cell type tumors are different from most adenocarcinomas of other types both clinicopathologically and immunohistochemically. Acta Pathol Jpn 41: 737-743, 1991.     

Lung adenocarcinoma with a micropapillary pattern: a clinicopathological study of 25 cases

Lung adenocarcinoma with a micropapillary pattern has recently been described, but its biological behavior is as yet uncertain. In this article we present a clinicopathological study of lung adenocarcinoma with micropapillary morphology. We selected 25 patients with lung adenocarcinoma with micropapillary morphology from the 2001-2004 pathology files (age range 54 to 81 years; mean 64.5 years). Micropapillary carcinoma is predominantly located at the periphery of the tumor nodule or mass and occurs irrespective of the subtype of the adenocarcinoma. A micropapillary component was seen against a mucinous background in three cases and microcalcifications resembling psammoma bodies were seen in one case. Four cases showed intensive invasive growth such as micropapillary adenocarcinoma of the breast and 21 showed alveolar type morphology with piling-up of the neoplastic cells with or without stromal invasion. Seven of twenty-three (30.4%) showed lymph node metastases at time of operation. Twelve of twenty-five (48%) showed pleural invasion. Regarding clinical outcome, 14 patients were alive without disease, 5 were alive with disease, and 5 died of the lung adenocarcinoma. No significant relationship was found between the extent of the micropapillary component and prognosis. However, the carcinoma seen in the five patients who died showed breast type histology with intensive invasive growth in three cases and alveolar type histology with intensive stromal invasion in two. Lung micropapillary carcinoma of breast type may behave more aggressively than the alveolar type.     

P16-positive continuous minimal deviation adenocarcinoma and gastric type adenocarcinoma in a patient with Peutz-Jeghers syndrome

We report a case of Peutz-Jeghers syndrome (PJS) in a 33-year-old female patient with synchronous uterine cervical minimal deviation adenocarcinoma (MDA) and gastric type adenocarcinoma (GTA). The patient was diagnosed with PJS at the age of 10. At the time of consultation, she complained of watery discharge. Magnetic resonance imaging of the pelvis showed a poorly circumscribed mass in the uterine cervix. Histologically, both MDA and GTA components, as well as their transitional area, were observed. Both components were diffusely positive for MUC6, CK7 and, robustly, for p16. Moreover, the components were negative for ER, PgR and CEA, while HIK1083 and CK20 positive cells were found focally. Ki-67 labeling index in the MDA component was 5% while that in the GTA component was 50%. This case of GTA accompanied by MDA in a patient with PJS is distinct from the single previously-reported comparable case of which we are aware, with respect to the overexpression of p16 protein, an event considered rare in these tumors, and the continuity between the MDA and GTA components. This continuity favors the hypothesis that GTA arises from the dedifferentiation of MDA.