共查询到19条相似文献,搜索用时 62 毫秒
1.
2.
本文用ABC-ELISA、HI和2-ME试验检测疑似乙脑病人101例双份血清,阳性率分别为75.25%、40.59%和27.72%。其中经HI和2-ME经典方法确诊的54例患者,用ABC-ELISA检测结果全部阳性,病程一周内阳性率为94.28%,血清抗体滴度最高可达1:25600,正常人和其他病人血清62份均呈阴性. 相似文献
3.
目的:分析经化学发光免疫分析法(Chemiluminescence immunoassay,CLIA)检测梅毒抗体在梅毒患者诊断中的应用价值.方法:收集本院2019年1月至2021年2月收治且需行早期梅毒诊断的125例患者的临床资料,以梅毒螺旋体明胶凝集试验(Treponema pallidum gelatin agglutination,TPPA)检测结果作为金标准,比较甲苯胺红不加热血清学试验(Unheated serological test of toluidine red,TRUST)、CLIA检测诊断梅毒的敏感性、特异性、准确性及CLIA检测诊断效果.结果:125例患者中,TPPA检测出18例阳性,检出率为14.40%.TRUST检测出10例,检出率为8.00%.CLIA检测出21例,检出率为16.80,明显高于TRUST检测(P<0.05).但CLIA、TPPA检测比较无差异(P>0.05).TRUST检测对早期梅毒诊断敏感性、特异性及准确性分别为61.11%、98.13%、92.80%;CLIA检测分别为100.00%、97.20%、97.60%.CLIA检测诊断早期梅毒的敏感性显著高于TRUST检测(P<0.05).CLIA检测S/CO为1~5、6~10、>10者经TPPA检测为阳性的符合率分别为80.00%、100.00%、100.00%.结论:经CLIA检测梅毒抗体在梅毒患者诊断中具有较高的敏感性、特异性,且操作简便快捷,具有较高临床应用价值. 相似文献
4.
抗体捕获ELISA检测包虫病人特异性IgM抗体及其应用的研究 总被引:1,自引:0,他引:1
用标记抗原、标记抗体、标记抗抗体及ABC四种形式的抗体捕获ELISA法检测包虫病人血清中特异性IgM抗体,结果以ABC-捕获ELISA法阳性率最高为52.5%,标记抗抗体法次之为39.6%,标记抗体法为22.5%,标记抗原法阳性率最低为20.7%。四种方法检测健康人血清均为阴性。手术前的包虫病人血清中特异性IgM抗体的阳性率明显高于手术后者,分别为52,2%和20%。不同寄生部位包虫病人特异性IgM抗体的阳性率没有明显差别。在入院就诊的病人中阳性率为35.4%,而在普查中发现的无症状病人中阳性率达70.5%,说明特异性IgM的检测在包虫病中也具有早期诊断的意义。 相似文献
5.
6.
7.
应用双抗原夹心ELISA法筛查献血员梅毒螺旋体抗体 总被引:1,自引:0,他引:1
目的:优选一种适宜于献血员梅毒筛查的试验方法。方法:采用检测梅毒特异性抗体的双抗原夹心ELISA法对献血员进行抗体检测,并与RPR检测结果进行比较,对两种方法检测结果不一致的标本,再用TPHA法进行确证。结果:ELISA法阳性检出率0.36%(41/1271)、RPR法阳性检出率0.26%(29/11271)。ELISA法与TPHA法总符合率97.5%(40/41)、RPR法与TPHA总符合率63.41%(26/41)。结论:ELISA法优于RPR法,具有较高的灵敏度和特异性,适宜于献血员的筛查.有利于控制和减少梅毒的输血传播。 相似文献
8.
用ELISA法检测丙型肝炎病毒IgM抗体试剂盒的研究 总被引:7,自引:1,他引:7
市售丙型肝炎检测试剂盒仅能检测丙肝IgG抗体,本工作建立的丙型肝炎病毒IgM抗体检测方法稳定性强,重复性好,检出率高,具有高度特异性,与甲、乙、丁型肝炎及CMV、EBV患者无交叉反应。健康人血中不含丙肝IgM抗体,我们用该方法检测了109例输血后肝炎患者,发现37例急性肝炎中36例丙型肝炎病毒IgM抗体阳性(97%),其中16例患者在起病后4个月内随病情好转而IgM抗体阴转,72例慢性输血后肝炎患者中43例ALT明显异常,43例中有36例(837%)丙肝IgM抗体阳性,而29例ALT正常患者仅有5例阳性,P<0.01,说明慢性肝炎丙肝IgM抗体阳性多伴有肝病活动。 相似文献
9.
抗体捕获ELISA法检测2446例孕妇血清中抗TORCH—IgM抗体 总被引:5,自引:3,他引:2
李芳芳 《中国优生与遗传杂志》1999,7(1):69-69
TORCH是一组具有致畸作用的病原徽物物。TO即Toxplasmagondi(刚地弓形虫);R即Rubellavirus(风疹病毒);C即cytomegalovirusⅠ、Ⅱ(单纯疟疾病毒Ⅰ、Ⅱ型),这一组病原微生物引起的感染,在围生医学中称为TORCH综合征。孕期感染TORCH5种病原体中的任何一种.都能通过胎盘或产道引起感染,导致流产死贴或胎儿生长迟缓、畸形.甚至新生儿感染或青春期发育障碍等严重后果。因此,对孕妇进行抗TORCH-IgM抗体检测,从优生优育角度是非常有必要的。目前,世界上许多地方已将TORCH检查列为常规端检项目。本文采用抗体捕获ELI… 相似文献
10.
11.
目的评价ELISA检测梅毒螺旋体(TP)抗体的分析性能,探讨未知诊断的定性实验的性能评价方法。方法参照临床实验室标准化协会(CLSI)发布的EP12-A2文件对ELISA法测定TP的临界值±20%范围的重复性进行分析,并与TP明胶凝集试验(TPPA)结果进行一致性比较。结果 +20%浓度临界值检测阳性率为≥95%,CV为14.8%;-20%浓度临界值检测阴性率≥95%,CV为19.6%;两种方法的一致程度百分比为99.6%,一致程度95%可信区间为98.8%~99.6%。结论 TP-ELISA法检测TP抗体的临界值±20%浓度范围之外标本,可得到可靠的检测结果。TP-ELISA法与TPPA法结果一致性好,可代替其作为TP诊断试验。 相似文献
12.
目的 评价化学发光微粒子免疫分析法(CMIA)用于梅毒螺旋体抗体(TP-Ab)筛查的价值,并制定合理的TP-Ab血清学筛查方案.方法 收集在我院进行TP-Ab筛查的患者标本,共计30,100例,对于CMIA为阴性者直接报告结果,CMIA阳性者采用梅毒螺旋体明胶凝集试验(TPPA)确认,并同时进行快速血浆反应素环状卡片试验(RPR),分析结果的一致性.结果 在30100例筛查标本中,CMIA阳性者402例(1.3%),样本吸光度与临界质控吸光度比值(S/CO)在1.05~40.56之间.CMIA结果>10 S/CO的标本共178例,TPPA阳性比例为100%,RPR阳性63例;在6-10 S/CO之间的标本共计50例,RPR阳性8例,TPPA阳性比例92%;在3~6 S/CO之间的标本59例,RPR阳性者5例,TPPA阳性的比例66.1%;在1~3 S/CO之间的标本115例,RPR均为阴性,TPPA阳性的比例仅为20%.结论 CMIA筛查TP-Ab具有较高的灵敏度,但特异性相对较低,采用CMIA进行TP-Ab筛查,并根据其S/CO值确定是否需要进一步采用另外一种高特异性梅毒螺旋体抗体检测试验进行确认,是TP-Ab筛查理想的方案,确认方法可以选择TPPA等试验.确认阳性者,应报告RPR结果,辅助临床区分既往感染和现症感染. 相似文献
13.
目的 分析我院老年患者梅毒螺旋体特异性抗体筛查情况,同时探讨其生物学假阳性的原因及临床意义.方法选取2016年1月至2016年12月我院8741例60岁以上老年患者,利用化学发光微粒子免疫检测法(CMIA)检测梅毒螺旋体特异性抗体,梅毒甲苯胺红不加热血清试验(TRUST)检测梅毒螺旋体非特异性抗体,同时采用免疫印迹法(Western Blot)进行梅毒确证.根据老年患者年龄将其分为60~69岁、70~79岁和80岁以上三个年龄组进行统计学分析.结果8741例60岁以上老年患者中有354例患者梅毒螺旋体特异性抗体阳性,60~69岁、70~79岁和80岁以上三个年龄组的梅毒螺旋体特异性抗体阳性率分别为3.42%、6.30%和6.02%,差异具有统计学意义(x2=31.236,P<0.001);同时,60~69岁与70~79岁年龄组比较,差异具有统计学意义(x2=28.932,P<0.001).240例老年患者CMIA筛查梅毒抗体阳性标本经TRUST和Western Blot检测都为阴性,且无临床症状和体征、无相关病史,结果判为假阳性.老年患者梅毒抗体假阳性人群主要分布在60~69岁.但随着老年患者年龄的增加,梅毒螺旋体抗体假阳性率也呈上升趋势,60~69岁、70~79岁和80岁以上三个年龄组的梅毒抗体假阳性率分别64.81%、71.43%和87.50%.老年患者梅毒螺旋体抗体生物学假阳性伴有的其他临床症状及指标异常主要包括癌症(55.36%)、溶栓剂或抗凝剂治疗(35.71%)、糖尿病(28.57%)、肝硬化(25.00%)、严重感染(25.00%)、肾病(17.86%)、自身抗体阳性(16.07%)、手术(14.29%)、肝炎(12.50%)和代谢紊乱(8.93%),各类原因相互影响,梅毒抗体检测假阳性S/CO值最高可达10.29.而梅毒螺旋体特异性抗体真阳性老年患者伴有的其他临床症状及指标异常百分比情况明显低于生物学假阳性患者.结论 对于CMIA梅毒筛查阳性而TRUST检测阴性的老年患者,需进行Western Blot检测并结合临床症状、体征和病史,作出最后诊断.老年患者随着年龄的增高,梅毒抗体的假阳性率也呈上升趋势,需注意癌症、自身免疫病、严重感染、代谢紊乱等可能会导致老年患者梅毒抗体检测生物学假阳性的因素,有助于临床医生分析检测结果并向患者作出合理解释. 相似文献
14.
Two different methods were used to prepare solid-phase antigen (Ag) from soluble extracts of tachyzoites of Toxoplasma gondii: (A) physical adsorption on polystyrene beads; and (B) formaldehyde fixation of Ag previously dried in microtitration wells. In both cases a horseradish peroxidase conjugate with anti-IgM IgG was used as tracer. The assay scheme consisted of sequential incubations of diluted serum samples and tracer solution (1 or 2 h, 37 degrees C), colour development in the presence of substrate (10 min at room temperature), addition of H2SO4, and absorbance reading at 492 nm. In procedure A no cut-off value for positives could be determined owing to a large overlap between positive and negative sera. The extent of overlap directly correlated with the total IgM content of samples. With negative sera similar values were obtained with sensitized and untreated beads: thus a correction could be made by directly subtracting absorbance values determined in parallel runs with uncoated beads. Results with negative sera correlated with total IgM concentration in procedure B also, but much less variability of blank values allowed negative and positive sera to be effectively discriminated. A series of reference positive and negative sera was correctly classified by both procedures A and B. However, the latter appeared preferable, as not requiring blank correction. 相似文献
15.
H Wulff L J Anderson M A Pallansch R P de Souza Carvalho 《Journal of medical virology》1987,21(4):321-327
Since outbreaks of severe acute hemorrhagic conjunctivitis occur worldwide [Hierholzer and Hatch, 1985] and the majority of the epidemics are caused by enterovirus 70 (EV-70), we developed an EV-70 IgM ELISA to simplify the diagnosis of these outbreaks. The test is based on the capture antibody technique and the use of monoclonal antibodies to EV-70. We detected EV-70 IgM antibodies in 55% of 76 convalescent-phase sera from an outbreak of acute hemorrhagic conjunctivitis in a Brazilian community. Among the 71 acute- and convalescent-phase serum pairs from this outbreak, 49 (69%) demonstrated a 4-fold or greater rise in neutralizing antibody-titer. The titer of IgM antibody began to drop by the fifth week after onset of illness. EV-70 IgM antibodies were not detected in 53 serum pairs with a 4-fold or greater rise in antibodies to other picornaviruses. The EV-70 ELISA proved to simple and relatively rapid to perform, appeared to be specific, and should be sensitive enough to diagnose outbreaks of EV-70 when multiple serum specimens can be tested. 相似文献
16.
目的 探讨环境温湿度对高通量酶联免疫吸附试验(ELISA)检测系统检测梅毒螺旋体抗体产生的影响。方法选择两种不同厂家的高通量ELISA检测系统,首先,对这两种检测系统进行性能验证。然后,自2012年12月1日2013年11月30日期间,每日应用室内定值弱阳性质控分别在这两种系统上对梅毒螺旋体抗体进行检测;记录每日室内温湿度及定值弱阳性质控的S/CO值。最后,对数据进行统计学分析。结果 对于高通量Addcare ELISA 600检测系统,根据温湿度不同进行分组,将各组定值弱阳性质控值(S/CO值)进行单因素方差分析,结果显示:按温度分组时差异具有统计学意义(F=6.05,P=0.0001),按湿度分组时差异具有统计学意义(F=3.23,P=0.0043);利用多变量线性回归分析温湿度对检测系统的影响,结果显示:在湿度相同的情况下,温度每升高1℃,S/CO值增加0.043,在温度相同的情况下,湿度每增加1%,S/CO值减少0.007。对于高通量Freedom evolzyer检测系统,根据温湿度不同进行分组,将各组定值弱阳性质控值(S/CO值)进行单因素方差分析,结果显示:按温度分组时差异具有统计学意义(F=30.27,P〈0.0001),按湿度分组时差异具有统计学意义(F=11.99,P〈0.0001);利用多变量线性回归分析温湿度对检测系统的影响,结果显示:在湿度相同的情况下,温度每升高1℃,S/CO增加0.037,在温度相同的情况下,湿度每增加1%,S/CO减少0.005。,结论(1)温度在18~22℃及湿度在20%~29%、30%~39%时检测值与预期值差异无统计学意义,温度在8~12℃、13~17℃,及湿度在10%~19%时检测值与预期值差异具有统计学意义;(2)温度与S/CO值成正相关,湿度与S/CO值成负相关。 相似文献
17.
ELISA assays for IgM and IgG rheumatoid factors 总被引:15,自引:0,他引:15
A Faith O Pontesilli A Unger G S Panayi P Johns 《Journal of immunological methods》1982,55(2):169-177
Enzyme-linked immunosorbent assays (ELISA) for the detection of IgM RF and IgG RF are described. A quantitation step was introduced into the IgM RF assay allowing expression of results as mg/ml of IgM RF. The effect of pepsin digestion of test sera on the IgG RF assay was established and the possible advantages of including pepsin digestion of sera in the assay considered. The assays have been developed to allow the screening of large numbers of sera in as rapid a time as possible. 相似文献
18.
目的 探讨化学发光法检测梅毒螺旋体特异性抗体的应用价值.方法 应用化学发光免疫测定法检测12503例住院患者梅毒螺旋体特异性抗体,并同ELISA、TPPA、RPR方法进行比较.结果 12503例住院患者化学发光法检出梅毒螺旋体特异性抗体阳性308例,同ELISA方法比较,阳性符合率99.08%,阴性符合率99.98%,总符合率99.93%.二者检测结果高度一致.同TPPA比较,阳性符合率98.7%.结论 化学发光免疫法检测梅毒特异性抗体具有特异性好、灵敏度高、结果易判断、便于自动化等优点,适合于梅毒的筛查. 相似文献
19.
Evaluation of recomWell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis 总被引:6,自引:0,他引:6
V. Sambri A. Marangoni M. A. Simone A. D'Antuono M. Negosanti R. Cevenini 《Clinical microbiology and infection》2001,7(4):200-205
Objective To evaluate the diagnostic performance of an enzyme immunosorbent assay (recomWell Treponema) for the diagnosis of syphilis. The novel recombinant antigens Tpn47, TpN17 and TpN15 were utilized.
Methods A total of 782 human serum specimens, belonging to four different categories (blood donors, n = 200; routine laboratory screening for syphilis, n = 400; syphilis patients, n = 122; potential cross-reactors, n = 60), were evaluated to compare the sensitivity and specificity of the recomWell Treponema kit with a standard whole Treponema pallidum cell lysate antigen-based ELISA (Syphilis Screening) and with micro-haemagglutination (MHA-TP).
Results The overall specificity and sensitivity of the recomWell Treponema IgG was 98.9% and 98.3%, respectively. The specificity and sensitivity of Syphilis Screening ELISA was 98.7% and 98.3%, respectively. The agreement between recomWell Treponema and Syphilis Screening was 100%, 97.8%, 95.9% and 95% among the blood donor specimens, screening samples, syphilis specimens and the potential cross-reactors, respectively. Values of concordance varying from 96.7% to 98.3% were found in the different groups of sera between recomWell Treponema and MHA-TP. In addition, recomWell Treponema demonstrated a good diagnostic performance when used to detect the IgM to T. pallidum . No false-positive sera were identified and, in 17/19 samples from primary infection, an IgM immune response was found.
Conclusions recomWell Treponema was shown to be a highly specific and sensitive method in all stages of syphilis screening and it can be considered as alternative to other ELISA tests based on native antigen preparations. 相似文献
Methods A total of 782 human serum specimens, belonging to four different categories (blood donors, n = 200; routine laboratory screening for syphilis, n = 400; syphilis patients, n = 122; potential cross-reactors, n = 60), were evaluated to compare the sensitivity and specificity of the recomWell Treponema kit with a standard whole Treponema pallidum cell lysate antigen-based ELISA (Syphilis Screening) and with micro-haemagglutination (MHA-TP).
Results The overall specificity and sensitivity of the recomWell Treponema IgG was 98.9% and 98.3%, respectively. The specificity and sensitivity of Syphilis Screening ELISA was 98.7% and 98.3%, respectively. The agreement between recomWell Treponema and Syphilis Screening was 100%, 97.8%, 95.9% and 95% among the blood donor specimens, screening samples, syphilis specimens and the potential cross-reactors, respectively. Values of concordance varying from 96.7% to 98.3% were found in the different groups of sera between recomWell Treponema and MHA-TP. In addition, recomWell Treponema demonstrated a good diagnostic performance when used to detect the IgM to T. pallidum . No false-positive sera were identified and, in 17/19 samples from primary infection, an IgM immune response was found.
Conclusions recomWell Treponema was shown to be a highly specific and sensitive method in all stages of syphilis screening and it can be considered as alternative to other ELISA tests based on native antigen preparations. 相似文献