Fluorimetric determination of dimethylaminohydroxypropoxytriamterene in biological fluids by direct evaluation of thin-layer chromatograms

A sensitive and specific thin-layer chromatographic method for the determination of the new antikaliuretic compound dimethylaminohydroxypropoxytriamterene (RPH 2823) in plasma and urine of rats is described. After dilution of the samples with methanol the tlc-separation is performed on silica gel plates in chloroform-methanol and NH3-atmosphere. RPH 2823 is recovered quantitatively from plasma and urine. The limit of detection is 5 ng/ml plasma and 50 ng/ml urine. The standard deviation of the method is 5% or less.     

Quantitative determination of imipramine and desipramine in human blood plasma by direct densitometry of thin-layer chromatograms

Imipramine and desipramine have been estimated in the plasma of patients receiving therapeutic doses of imipramine. The compounds were extracted as bases into n-heptane, separated by thin-layer chromatography and transferred into coloured spots by an oxidative procedure. The quantification was made with a new densitometric technique of high sensitivity. The coefficient of variation was 11–16% at concentrations of 20 μg litre^?1 (71m?2 nmol and 75m?0 nmol litre^?1 for imipramine and desipramine respectively) and the recovery was 70–81%.     

荧光分光光度法测定盐酸洛美沙星胶囊含量

采用荧光分光光度法测定洛美沙星胶囊的含量。激发／发射波长为２８７／４５５ｎｍ，平均回收率为９８．０％，ＲＳＤ为０．９８％。在０．１～１．０μｇ／ｍｌ范围内，其荧光强度与浓度线性关系良好。     

On the determination of triamterene, hydroxythiamterene and hydroxytriamterene sulphate ester in biologica material by direct evaluation of thin-layer chromatograms (author's transl)]

The quantitative measurement of 2,4,7-triamino-6-phenylpteridine (triamterene), as well as its metabolites hydroxytriamterene and hydroxytriamterene sulphate ester, is described. Estimation of urine, plasma and faecal samples was achieved by a direct fluorometric method following separation on kieselgel plates. The procedure allows the simultaneous assay of the three substances on the plate without the need for previous chromatographic separation or derivatisation.     

Quantitative determination of drugs by means of densitometry of thin-layer chromatograms. Part 4: Determination of vitamins A and E in pharmaceutical preparations.

A quantitative method for the determination of vitamins A and E is proposed based on the direct densitometry of thinlayer chromatographic spots. An appropriate solvent system for the chromatographic separation of the vitamins and the conditions under which a linear relationship between the absorbed light and the logarithm of concentration exists (1--10 mug range) are found. The precision of the method is evaluated by a statistical treatment of the results. Satisfactory reproducibility is established-- the variation coefficient for the determination of the two vitamins is about 4--5%. The method is applied for the analysis of oil and aqueous injection solutions, mixtures containing the three vitamins A, E and D2 and some pharmaceutical preparations (Pharmavit? and Devitforte?). The presence of the decomposition products and other substances (other pharmaceuticals, stabilizers, solubilizers etc.) does not affect the determination. The analysis is performed without preliminary treatment of the sample.     

川明参的HPLC指纹图谱

目的 研究川明参药材的指纹图谱.方法 用RP-HPLC法测定了10个不同产地批次川明参药材样品.采用Phe-nomenex Luna C<,18>柱(250 mm×4.6 mm,5 μm);流动相为乙腈-0.02%磷酸;检测波长230 nm,柱温25℃.结果 建立了川明参药材的HPLC指纹图谱,标定了12个共有指纹峰.结论 所建方法 稳定、可靠、重复性好,指纹图谱专属性强.     

山海丹牌野菊花注射液特征图谱分析

目的 建立山海丹牌野菊花注射液中有效成分的含量测定及特征图谱研究方法,对其质量进行控制。方法 采用高效液相色谱法(HPLC)梯度洗脱,流动相:乙腈-0.5%磷酸溶液,梯度洗脱,流速:1.0 mL·min-1,检测波长326 nm,以木犀草苷为参照峰计算其共有峰的相对保留时间。结果 标记8个吸收值较大的共有峰,9批供试品中S峰与标准对照品峰相对应,其余特征峰的相对保留时间RSD均小于0.6%。结论 该特征图谱方法可行,可用于该厂家野菊花注射液的质量控制。