Novel HLA-A*11 allele, A*1120, identified by sequence-based typing

In this report, we describe the identification of a human leucocyte antigen-A*11 (HLA-A*11) nucleotide sequence variant, a new HLA-A*1120 by using sequence-based typing (SBT). The new allele was detected during routine HLA typing by high-resolution SBT. Allele A*1120 showed one nucleotide difference with A*110101 at codon 152 (GCG-->GAG) resulting in an amino acid change from alanine to glutamate. Residue 152 is located on alpha(2)-helix of HLA class I molecule and involved in peptide binding by constructing E pocket of peptide-binding groove, implying that the change of the residue 152 would affect the binding affinity of peptides to A*1120 allele.     

Novel HLA-A*31 allele, A*3111 identified by sequence-based typing

In this report, we describe the identification of an HLA-A*31 nucleotide sequence variant, a new HLA-A*3111, in three members of a Korean family by using sequence-based typing (SBT). The new allele was detected during routine HLA typing by high-resolution SBT. Allele A*3111 showed one nucleotide difference with A*310102 at codon 165 (GTG-->CTG) resulting in an amino acid change from valine to leucine (V165L). Serologic reactivity was shorter than normally expected.     

A new HLA-A*24 variant, A*2475, identified by sequence-based typing in the Korean population

A novel human leukocyte antigen (HLA) A*24 allele was identified in the Korean population and designated HLA-A*2475. The HLA-A*2475 allele shows one nucleotide difference from A*24020101 in exon 3 at nucleotide position 575 (T→C), resulting in an amino acid change, Leu168Arg.     

HLA-A*2468, a new allele identified by sequence-based typing in the Chinese population

We report here the identification of a novel human leukocyte antigen-A*2468 allele that was detected by polymerase chain reaction sequence-based typing.     

Identification of a novel allele HLA-A*9208 by sequence-based typing

A novel HLA-A*9208 allele was identified in a Chinese individual by polymerase chain reaction sequence-based typing.     

A novel HLA-A*3315 allele identified by haplotype-specific extraction and sequence-based typing

A novel HLA-A*33 variant allele, A*3315, was identified in three Korean siblings. This allele shows only one nucleotide difference from A*330301 in exon 4 at nucleotide position 727 (codon 219 CGG-->TGG), resulting in an amino acid change from arginine (R) to tryptophan (W).     

A novel HLA-DRB1 allele, DRB1*0832, identified by sequence-based typing method

Human leukocyte antigen-DRB1*0832 may have arisen by gene conversion from a DRB1*080302 backbone, leading to amino acid changes at codons 26, 28, 30, 32, 37, and 38.     

Identification of a new HLA-A*11 allele, HLA-A*1135, by sequence-based typing in Korean individual

A novel human leukocyte antigen (HLA)-A allele has been identified in the cord blood of a Korean baby. New HLA-A*1135 allele was different from HLA-A*1131 by three nucleotide substitution at codon 142 (ATC→ACC) and codon 163 (CGG→ACG), resulting in two amino acid change, Ile 142 Thr and Arg 163 Thr.     

HLA-A*2634, a new allele identified by sequence-based typing in the Korean population

A new HLA-A*2634 allele differs from A*260101 by a change from C to T at the nucleotide 559 of exon 3, with a coding change R163W.     

Identified a novel allele HLA-A*240212 by sequence-based typing

A novel human leukocyte antigen-A allele, A*240212, has been identified during routine sequence-specific oligonucleotide typing and sequence-based typing in a sample from a registered donor of Chinese Marrow Donor Program. The A*240212 allele differs from the most closely matching allele A*24020101 by one nucleotide substitution in Exon 2, at position 230, while the nucleotide exchange is silent mutation.     

Identification of the novel HLA-A*9250 allele by sequence-based typing in a Caucasian bone marrow donor from Italy

HLA-A*9250 allele was identified by SBT in a Caucasian bone marrow donor. It differs from the closest A*020101 by only one nucleotide (A→G) at position 124 in exon 2 (Arg to Gly at codon 18); this is an uncommon variation at a highly conserved nucleotide position, located on the loop between S1-S2 β-sheets in α1 domain.     

Identification of a novel allele HLA-A*9206 by sequence-based typing in the Chinese population

A novel human leukocyte antigen-A*9206 allele was identified by sequence-based typing in China.     

Identification of a new HLA-A*24 variant, A*2474, by sequence-based typing in the Korean population

A novel human leukocyte antigen, A*24 (HLA-A*24), was identified in the Korean population. HLA-A*2474 allele shows one nucleotide difference from A*24020101 in exon 2 at nucleotide position 186 (C → A), resulting in an amino acid change, Ser38Arg.     

A novel HLA-Cw*03 allele, Cw*033802, identified by sequence-based typing

New allele Cw*033802 showed one nucleotide difference with Cw*0338 at codon 99 (TAC-->TAT).     

A novel HLA-B*46 allele, B*4609, identified by sequence-based typing in a Chinese donor

A novel human leukocyte antigen-B allele, B*4609, has been identified. The B*4609 allele has one nucleotide change from the closest matching allele B*460101 resulting in an amino acid change from E (GAG) to V (GTG) at codon 176.     

Haplotype-specific sequence-based typing shows the novel allele HLA-A*030105

The novel allele human leukocyte antigen (HLA)-A*030105 differs from HLA-A*030101 by a synonymous nucleotide exchange at codon 87 in exon 2 (G→A).     

A new HLA-A*26 variant, A*2637 identified by haplotype-specific extraction and sequencing-based typing

The novel allele A*2637 differs from the common A*260101 by a single nucleotide substitution in exon 2 (position 186 C>G) causing an amino acid exchange (SER>ARG).