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1.
Prasanna Lokadolalu Chandracharya Rohini Alva Anjaneyalu Konuri Ashok Kumar 《Journal of the Anatomical Society of India》2018,67(1):80-85
Introduction
The present study was undertaken to know the effect of folic acid (FA) supplementation and FA absence diet during pregnancy on the number of implantation as well as their endometrial changes.Methods
Eighteen Wistar strain Albino rats were randomly divided into three groups and given different diets: Control group with normal diet, group with FA supplementation diet, and group with FA absent diet (with added Succinyl Sulfathiazole) for 5 weeks. The number of coloured thickenings along the uterine horns were identified by injecting 1% Evan’s blue due solution. The sites of embryo implantation were fixed in 10% buffered formalin for 8 hours and embedded.Result
Average number of implantation observed in pregnant dams fed with FA supplementation diet was 10 in contrast it was 7 in diet with FA absent diet and 8 in control groups. Histologically, implantation site in FA supplemented group showed favorable endometrial environment than the other groups.Discussion
FA is essential in successful implantation by providing favorable receptive environment to receive the implantation-competent blastocyst for a successful pregnancy. 相似文献2.
3.
Adeline Bernier Adam Yattassaye Dominic Beaulieu-Prévost Joanne Otis Emilie Henry Jorge Flores-Aranda Lyne Massie Marie Préau Bintou Dembélé Keita 《Patient education and counseling》2018,101(2):248-255
Objective
The objective of this study was to assess the short-term effects of Gundo-So—a program aimed at empowering Malian women living with HIV (WLHIV) regarding serostatus disclosure management.Methods
A pre-experimental study with two measures (one week before and four weeks after Gundo-So) was carried out. A 35-item questionnaire was administered to a convenience sample of 210 WLHIV. Six outcomes were considered: ability to decide whether or not to disclose HIV status, self-efficacy to keep HIV status a secret, self-efficacy to disclose HIV status, feeling crushed by the weight of secrecy, perceived physical health, and perceived psychological health. For each outcome, temporal changes associated with the intervention were assessed using linear regressions with random intercepts.Results
Statistically significant change was observed for all six outcomes between the pre- and post-intervention measures. Furthermore, several variables were associated with the baseline levels of the outcomes and the intervention effect.Conclusion
The results suggest that Gundo-So empowers Malian WLHIV with regard to serostatus disclosure management, thus improving their perceived physical and psychological health.Practical implications
These results highlight the need for programs to empower WLHIV regarding serostatus disclosure, so that WLHIV can make free and informed decisions regarding serostatus disclosure. 相似文献4.
Mary Boyde Jenny Rankin Jennifer A. Whitty Robyn Peters Julie Holliday Charmaine Baker Rita Hwang Donna Lynagh Dariusz Korczyk 《Patient education and counseling》2018,101(12)
Objective
To elicit patients' preferences for cardiac rehabilitation(CR).Methods
A Discrete Choice Experiment was used to quantify patients' preferences for the delivery of CR. This survey-based method elicited the relative importance of different characteristics of a program.Results
200 in-patients eligible to attend CR completed the survey. Over half of the patients strongly preferred a centre-based compared to a home-based program. Many but not all preferred a program starting within two rather than six weeks of discharge and exercise delivered in a group rather than individual setting, with exercise via the internet using telehealth strongly disliked. Some respondents preferred lifestyle information delivered one-to-one by a health professional, and there was an overall preference against delivery by smart phone Apps. Some preferred a program out of rather than within working hours and a shorter program (four weeks compared to eight weeks).Conclusions
This study provides further insight into patient preferences for a CR program. Although the strongest preferences were for centre-based programs with healthcare professionals facilitating exercise classes and one-on-one education, it is important to offer flexible delivery as one approach will not suit everyone.Practice Implications
There is the potential to improve CR programs by focusing on patient preferences. 相似文献5.
Tuğba Kotil Şengül Şentürk Türkan Sarioğlu Leyla Tapul 《Experimental and toxicologic pathology》2017,69(8):643-648
Aims
Retinoic acid (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated.Methods
Twenty-four adult Spraque Dawley rats were divided into two groups, the experimental group was treated with 40 mg/kg/day 13-cis RA for 5 days by gavage. Uterine tissue sections were treated with BrdU and MMP-2 antibodies, evaluated using light microscopy. Tissues were fixed with 2.5% glutaraldehyde and evaluated using transmission electron miroscopy.Results
MMP-2 immunoreactivity decreased in the stromal cells compared with the control group and no staining of MMP-2 was observed in glandular epithelium in the experimental group. BrDU labeling of cells showed significant decrease in RA-treated group versus control group cells. Based on the electron microscopy evaluation, the surface epithelial cells of the experimental group showed vacuolization, and an accumulation of lipofuscin bodies was also observed in the gland epithelium. Cells involving autophagic vacuoles contained excess lipid granules in the entire uterus layers especially localized at the border of the endometrium and myometrium.Conclusion
RA had negative effects on cell proliferation and cell morphology and inhibited MMP-2 expression. 相似文献6.
Annemiek J. Linn Liset van Dijk Julia C.M. van Weert Beniam G. Gebeyehu Ad. A. van Bodegraven Edith G. Smit 《Patient education and counseling》2018,101(8):1419-1426
Objective
Improving adherence is a challenge and multiple barriers are likely to explain non-adherence. These barriers differ per patient and over course of the regimen. Hence, personalized interventions tailored to the specific barriers are needed. In a theoretical and evidence-based Tailored Multimedia Intervention, technology (online preparatory assessment, text messaging) was used as an add-on to a tailored counseling session (learned during a communication skills training), with the expectation of synergistic effects.Methods
A cluster randomized controlled trial was conducted in six hospitals, eight nurses and 160 chronic patients. Patient satisfaction with communication, beliefs about medication, self-efficacy and medication adherence were assessed at initiation of the treatment and after six months.Results
Intervention effects were found for patient satisfaction with nurses’ affective communication and self-efficacy at the initiation of treatment. The effect on self-efficacy remained after six months.Conclusion
By combining tailored counseling with technology, this intervention resulted in positive changes in important prerequisites of medication adherence.Practical implications
Technology can contribute significantly to health care providers’ ability to tailor information to the patients’ needs. 相似文献7.
Purpose
Barrett's esophagus is one of the main risk factors for increased incidence of esophageal adenocarcinoma. In this study, we studied protein expression levels and cellular localizations of MUC-1, MUC-2, MUC-5AC, CK7, and cytoplasmic p27 to assess the relationship between the expression of each of these proteins and the disease progression on endoscopic biopsies.Materials and methods
Immunohistochemical analyses were performed using antibodies produced against MUC-1, MUC-2, MUC-5AC, CK7, and p27. Endoscopic specimens of esophageal mucosa were obtained from 72 patients who underwent esophagectomy for Barrett’s esophagus, metaplasia, dysplasia, or esophageal adenocarcinoma developed from Barrett’s esophagus.Results
Multilayer squamous epithelium showed only MUC-1 positivity in the EAC group while MUC-2 and MUC-5AC staining could not be detected in this group. Strong and diffused membranous or cytoplasmic staining of CK7 was observed at squamous, ductal, surface columnar and/or glandular epithelium. c-p27 staining was diffused and moderate in the cellular membranes observed in all groups except for esophageal epithelial metaplasia without intestinal metaplasia. Additionally, weakly focal cytoplasmic staining in squamous epithelium of p27 in EAC was detected.Conclusions
Barrett's esophagus, which has a heterogeneous epithelium, might yield different diagnosis based on endoscopic evaluation and immunohistological investigation. Thus, the use of MUC1, p27, and CK7 might strengthen the truthful diagnosis. MUC-1, CK7, and c-p27 immunostaining can be used as the predictive markers for esophageal cancer progression from Barrett’s esophagus. 相似文献8.
Fernanda Rodrigues Helmo Angela Maria Moed Lopes Anna Cecília Dias Maciel Carneiro Carolina Guissoni Campos Polyana Barbosa Silva Maria Luíza Gonçalves dos Reis Monteiro Laura Penna Rocha Marlene Antônia dos Reis Renata Margarida Etchebehere Juliana Reis Machado Rosana Rosa Miranda Corrêa 《Pathology, research and practice》2018,214(1):7-14
9.
Johanna Granhagen Jungner Elisabet Tiselius Marika Wenemark Klas Blomgren Kim Lützén Pernilla Pergert 《Patient education and counseling》2018,101(9):1661-1668
Objective
To develop a valid and reliable questionnaire addressing the experiences of healthcare personnel of communicating over language barriers and using interpreters in paediatric healthcare.Methods
A multiple- methods approach to develop and evaluate the questionnaire, including focus groups, cognitive interviews, a pilot test and test-retest. The methods were chosen in accordance with questionnaire development methodology to ensure validity and reliability.Results
The development procedure showed that the issues identified were highly relevant to paediatric healthcare personnel and resulted in a valid and reliable Communication over Language Barriers questionnaire (CoLB-q) with 27 questions.Conclusion
The CoLB-q is perceived as relevant, important and easy to respond to by respondents and has satisfactory validity and reliability.Practice implications
The CoLB-q can be used to map how healthcare personnel overcome language barriers through communication tools and to identify problems encountered in paediatric healthcare. Furthermore, the transparently described process could be used as a guide for developing similar questionnaires. 相似文献10.
Taghi Meskari Soraya Ghafari Vahid Khouri Ramin Azarhoush Mohammad Jafar Golalipour 《Journal of the Anatomical Society of India》2018,67(1):35-39
Introduction
Previous study has shown the adverse effects of type 1 and 2 diabetes mellitus on male reproductive system. This study was done to evaluate the effect of induced gestational diabetes on seminiferous tubule of 12 weeks age offspring rats.Methods
10 Wistar rats' dams were randomly allocated to control and diabetic groups. Streptozotocin was used to induce diabetes in female rats. Dams in diabetic group received 40 mg/kg/BW of streptozotocin at the first day of gestation and control group animals received an equivalent volume of normal saline by intraperitoneally. Six offspring of each group were randomly selected on day 84 postnatal. Five micrometer sections were taken from testes, stained with hematoxylin and eosin. Photographs of sections were taken using Olympus BX51 microscope and a digital camera DP12. Density and number of spermatogenesis cells, leydig cells, sertoli cells, seminiferous tubule diameter and Seminiferous epithelial height and dUTP end-labeling positive cells were evaluated in 50,000 μm2 area of seminiferous tubules by Olysia Autobioreport software.Results
Spermatogenesis and leydig cells in gestational diabetic offsprings non-significantly reduced in compare to controls. Sertoli cells significantly reduced in gestational diabetic offspring compared to controls. Seminiferous tubular diameter and seminiferous epithelial height non-significantly reduced in gestational diabetic offspring compared to controls. The apoptotic cells in diabetic group non-significantly increased in comparison with controls. The histopathological alterations were not seen in experimental group.Discussion
Uncontrolled gestational diabetes significantly reduces the sertoli cells but non-significantly reduces the spermatogenic cells in the rat offsprings. 相似文献11.
Barbora Silarova Fiona E. Douglas Juliet A. Usher-Smith Job G. Godino Simon J. Griffin 《Patient education and counseling》2018,101(1):43-51
Objective
To identify the proportion of individuals with an accurate perception of their risk of type 2 diabetes (T2D) prior to, immediately after and eight weeks after receiving a personalised risk estimate. Additionally, we aimed to explore what factors are associated with underestimation and overestimation immediately post-intervention.Methods
Cohort study based on the data collected in the Diabetes Risk Communication Trial. We included 379 participants (mean age 48.9 (SD 7.4) years; 55.1% women) who received a genotypic or phenotypic risk estimate for T2D.Results
While only 1.3% of participants perceived their risk accurately at baseline, this increased to 24.7% immediately after receiving a risk estimate and then dropped to 7.3% at eight weeks. Those who overestimated their risk at baseline continued to overestimate it, whereas those who underestimated their risk at baseline improved their risk accuracy. We did not identify any other characteristics associated with underestimation or overestimation immediately after receiving a risk estimate.Conclusion
Understanding a received risk estimate is challenging for most participants with many continuing to have inaccurate risk perception after receiving the estimate.Practice implications
Individuals who overestimate or underestimate their T2D risk before receiving risk information might require different approaches for altering their risk perception. 相似文献12.
Pål Gulbrandsen 《Patient education and counseling》2018,101(11):1990-1994
Objective
To direct attention to the concept of vulnerability, how it affects interactions in subtle ways and is difficult to detect in studies of clinical dialogues.Methods
A reflection on three everyday examples of what seems like insignificant details in physician-patient interaction, enlightened by readings with an emphasis on unacknowledged shame, illness and social bonds, and the physician’s role.Results
Physicians are aware of patients’ vulnerability as risk and susceptibility to harm under certain circumstances, but often miss the vulnerability related to perceived or anticipated loss of social bonds. The latter may elicit unacknowledged shame, which leads to subtle behavioral changes that are easy to overlook. Typical reactions are silence or lack of relevant response to questions. Physicians are rarely aware of their own vulnerability. Slight behavioral changes from a clinician may reduce or increase a patient’s ability to partake actively in problem solving and decision-making.Conclusion
When physicians or patients are touching upon unacknowledged shame as part of being vulnerable, subtle changes in the interaction may hamper efficient communication.Practice implications
We need studies that add participants’ unprompted and prompted reflections on encounter videos, with an emphasis on micro-events and their explanation and impact on the interaction. 相似文献13.
Sharon A. Brown Alexandra A. García Julie A. Zuñiga Kimberly A. Lewis 《Patient education and counseling》2018,101(6):1036-1050
Purpose
The primary purpose is to review diabetes workplace interventions and the degree to which they improve diabetes-related outcomes in employees diagnosed with or at risk for T2DM.Methods
Three electronic databases and ancestry searches were used to identify peer reviewed articles published in English from 2000 to June 2017.Results
The number of participants represented by the 22 selected studies, excluding one large outlier, was 4243. On average, the samples were 57% female and ethnically diverse. Interventions—healthy eating behaviors, physical activity, and/or monitoring and self-managing diabetes and cardiovascular risk factors—were delivered in group sessions of fewer than 20 employees. Programs involved 1-h weekly sessions held during lunch hour or at other times during the workday for 12 to 24?weeks. Study outcomes, commonly measured at 6 and/or 12 months, were consistently positive.Conclusion
The literature search uncovered beginning evidence that workplace interventions hold promise for preventing diabetes and/or its complications. More rigorous, creatively designed, workplace studies, are needed for employees at high-risk for developing diabetes.Practice implications
Implications include the need for employer education about the benefits of employer support for such programs and attention to motivational strategies so employees will take full advantage of programs that are offered. 相似文献14.
Evaluation of NCAM and c-Kit as hepatic progenitor cell markers for intrahepatic cholangiocarcinomas
Jing Xu Yanhong Tan Xiaoxia Shao Cuiming Zhang Yanling He Jie Wang Yanfeng Xi 《Pathology, research and practice》2018,214(12):2011-2017
Background
Intrahepatic cholangiocarcinomas (ICCs) are primary liver malignancies and are the second most common type of malignancy after hepatocellular carcinoma. ICCs are heterogeneous in clinical features, genotype, and biological behavior, suggesting that ICCs can initiate in different cell lineages.Aim
We investigated intrahepatic cholangiocarcinoma RBE cell lines for the markers neural cell adhesion molecule (NCAM) and c-Kit, which possess hepatic progenitor cells properties.Methods
NCAM?+?c-Kit?+?cells were tested for hepatic progenitor cell properties including proliferation ability, colony formation, spheroid formation, and invasiveness in NOD/SCID mice. The Agilent Whole Human Genome Microarray Kit was used to evaluate differences in gene expression related to stem cell signaling pathways between NCAM?+?c-Kit?+?and NCAM-c-Kit- subset cells. Microarray results were further confirmed by real-time RT-PCR.Results
NCAM?+?c-Kit?+?cells showed hepatic progenitor cell-like traits including the abilities to self-renew and differentiate and tumorigenicity in NOD/SCID mice. Differences were observed in the expression of 421 genes related to stem cell signaling pathways (fc ≥ 2 or fc ≤ 0.5), among which 231 genes were upregulated and 190 genes were downregulated.Conclusion
NCAM?+?c-Kit?+?subset cells in RBE may have properties of hepatic progenitor cells. NCAM combined with c-Kit may be a valuable marker for isolating and purifying ICC stem/progenitor cells. 相似文献15.
Farina Kokab Sheila Greenfield Antje Lindenmeyer Manbinder Sidhu Lynda Tait Paramjit Gill 《Patient education and counseling》2018,101(4):619-630
Objective
The objective of this research was to synthesise qualitative literature about the perceived influence and experience of social support, in relation to cardiovascular disease (CVD) prevention in migrant Pakistani communities.Methods
Articles were systematically reviewed, critically appraised, and analysed using an adapted meta-ethnography approach.Results
Sixteen qualitative studies on health behaviours related to CVD prevention were included.Findings
include four sub-themes under two substantive thematic areas that focus on: 1) family dynamics and 2) community dynamics influenced by discrimination. For members of the Pakistani community, gendered family dynamics and discrimination from outside and within community networks influenced behaviour change.Conclusion
The authors of the synthesis developed multi-layered, contextualised interpretations of the care needs of an established multi-generational community. Future qualitative studies taking an intersectional approach to interpreting the role of social networks in migrant communities should take into account gender, identity, culture and faith.Practice implications
Health care providers should focus on cultural awareness and sensitivity during consultations. In particular, general practitioners can benefit from the insight they gain from patient experiences, allowing for more appropriate recommendations. 相似文献16.
Miyako Shimasaki Yoshimitsu Kanazawa Katsuaki Sato Hiroyuki Tsuchiya Yoshimichi Ueda 《Pathology, research and practice》2018,214(1):80-88
Background and aim
Recent studies of several carcinomas have reported that aquaporin possesses novel oncogenic properties. The aim of this study was to clarify the involvement of aquaporin-1 and ?5 in the proliferation, invasion and metastasis of soft tissue sarcomas.Materials and methods
The expression of aquaporin-1 and -5 was immunohistochemically examined in 73 soft tissue sarcomas as well as in benign, locally aggressive soft tissue tumors, and in soft tissues of adult humans and human fetuses. The mRNA and protein expression of aquaporin-1 and -5 genes were quantified in 19 sarcoma tissues.Results
Aquaporin-1 was expressed in the tumor cells of 37 (51%) and aquaporin-5 in 29 (40%) of 73 soft tissue sarcomas. Two expression patterns were identified: a differentiation-dependent pattern, similar to their expression in adult human soft tissue and in benign soft tissue tumors, and an aggressiveness-related pattern, that is similar to their expression in the mesenchymal cells of the developing fetal limb. The latter expression pattern proved to be an independent prognostic factor for patients with soft tissue sarcoma, in which aquaporin-1 was related to the invasiveness, and aquaporin-5 to the proliferation of soft tissue sarcoma cells.Conclusion
These results indicate pivotal roles for aquaporin-1 and -5 in the aggressive growth and metastatic potential of soft tissue sarcomas, suggesting that they are promising targets for the treatment of patients with intractable soft tissue sarcoma. 相似文献17.
Rosa Geurtzen Jos Draaisma Rosella Hermens Hubertina Scheepers Mallory Woiski Arno van Heijst Marije Hogeveen 《Patient education and counseling》2018,101(12)
Objective
To investigate experienced and preferred prenatal counseling among parents of extremely premature babies.Methods
A Dutch nationwide, multicenter, cross-sectional study using an online survey. Surveys were sent to all parents of extremely premature babies born between 2010 and 2013 at 24+0/7–24+6/7 weeks of gestation.Results
Sixty-one out of 229 surveys were returned. A minority (14%) had no counseling conversation. Conversations were done more often by neonatologists (90%) than by obstetricians (39%) and in 37% by both these experts. Supportive material was rarely used (19%). Mortality (92%) and short-term morbidity (88%) were discussed the most, and more frequently than long-term morbidity (65%), practical items (63%) and delivery mode (52%). Most decisions on active care or palliative comfort care were perceived as decisions by doctor and parents together (61%). 80% felt they were involved in decision-making. The preferred way of involvement in decision-making varied among parents.Conclusion
The vast majority of parents were counseled: mostly by neonatologists, and mainly about mortality and short-term morbidity. Parents wanted to be involved in the decision-making process but differed on the preferred extent of involvement.Practice implications Understanding of shared decision-making may contribute to meet the various preferences of parents. 相似文献18.
Miriam Komaromy Erin Fanning Madden Andrea Zurawski Summers Kalishman Kristin Barker Patricia O’Sullivan Martin Jurado Sanjeev Arora 《Patient education and counseling》2018,101(3):524-531
Objective
Elicit patients’ perceptions of factors that facilitate their engagement in careMethods
In-depth interviews with 20 adult Medicaid patients who had complex health problems, frequent hospitalizations/emergency department use, and who were enrolled in an intensive, team-based care program designed to address medical, behavioral, and social needs.Results
Prior to engaging in the program, participants described weak relationships with primary care providers, frequent hospitalizations and emergency visits, poor adherence to medications and severe social barriers to care. After participating in the program, participants identified key factors that enabled them to develop trust and engage with care including: availability for extended intensive interactions, a non-judgmental approach, addressing patients' material needs, and providing social contact for isolated patients. After developing relationships with their care team, participants described changes such as sustained interactions with their primary care team and incremental improvements in health behaviors.Conclusion
These findings illuminate factors promoting “contingent engagement” for low socio-economic status patients with complex health problems, which allow them to become proactive in ways commensurate with their circumstances, and offers insights for designing interventions to improve patient outcomes.Practice implications
For these patients, engagement is contingent on healthcare providers’ efforts to develop trust and address patients’ material needs. 相似文献19.
Birte Østergaard Romy Mahrer-Imhof Lis Wagner Torben Barington Lars Videbæk Jørgen Lauridsen 《Patient education and counseling》2018,101(8):1385-1393
Objective
To evaluate the short-term (3 months) effects of family nursing therapeutic conversations (FNTC) on health-related quality of life, self-care and depression in outpatients with Heart failure (HF).Methods
A randomised multi-centre trial was conducted in three Danish HF clinics. The control group (n?=?167) received usual care, and the intervention group (n?=?180) received FNTCs as supplement to usual care. Primary outcome was clinically significant changes (6 points) in Kansas City Cardiomyopathy Questionnaire (KCCQ) summary score between groups. Secondary outcomes were changes in self-care behaviour and depression scores. Data were assessed before first consultation and repeated after three months.Results
No statistically significant difference was found in the change of KCCQ, self-care and depression scores between the groups. KCCQ scores of patients in the FNTC group changed clinically significant in seven domains, compared to one domain in the control group, with the highest improvement in self-efficacy, social limitation and symptom burden.Conclusion
FNTC was not superior to standard care of patients with HF regarding health-related quality of life, self-care and depression.Implication for practice
Addressing the impact of the disease on the family, might improve self-efficacy, social limitation and symptom burden in patients with heart failure. 相似文献20.
Katarina Vukojevic Fila Raguz Marijan Saraga Natalija Filipovic Ivana Bocina Darko Kero Merica Glavina Durdov Vlatka Martinovic Mirna Saraga-Babic 《Acta histochemica》2018,120(8):748-756