高效液相色谱法同时测定沙日-嘎-4中栀子苷和盐酸小檗碱的含量

目的:采用高效液相色谱法同时测定蒙药沙日-嘎-4中栀子苷、盐酸小檗碱的含量。方法:Kromasil C18色谱柱(4.6 mm×250 mm 5μm),乙腈-0.2%磷酸水溶液梯度洗脱,流速1 ml/min,柱温30℃,检测波长为238 nm(栀子苷)、343 nm(盐酸小檗碱)。结果:栀子苷、盐酸小檗碱的线性范围分别为5.8~52.2μg/ml(r=0.9999),2.6~23.4μg/ml(r=0.9997);栀子苷的平均加样回收率为100.53%,RSD为0.95%(n=6);盐酸小檗碱的平均加样回收率为100.34%,RSD为1.36%(n=6)。结论:采用本研究方法同时测定沙日-嘎-4中栀子苷、盐酸小檗碱的含量,结果准确,重复性、回收率好,可用于蒙药复方制剂沙日-嘎-4的质量评价和控制。     

四逆散中芍药苷含量的HPLC法测定

目的 建立四逆散中芍药苷的HPLC测定法.方法 用C18柱,流动相为乙腈-水(1:4),检测波长为230nm,流速为 1.0ml/min,柱温:40℃.结果 芍药苷进样量在0.168-0.672μg范围内线性关系良好(r=0.9998),平均加样回收率为102.0% (RSD=1.0%).结论 本方法简便、快速、准确、重现性好,可用于四逆散中芍药苷的含量测定.     

高效液相色谱法测定复肾宁胶囊中栀子苷的含量

目的:探寻复肾宁胶囊中栀子苷的含量测定方法.方法:采用高效液相色谱法测定栀子苷的含量,色谱柱Hypersil ODS2柱,流动相为乙腈-水(12∶ 88),流速1.0 ml/min,检测波长238 nm.结果:该方法线性关系良好,平均加样回收率为99.07%,RSD=1.00%(n=6).结论:本方法简便、快速、精密度高、分离度良好,可用于复肾宁胶囊的质量控制.     

HPLC同时测定小儿豉翘清热颗粒中两种苷含量

目的:建立小儿豉翘清热颗粒中栀子苷、黄芩苷含量的测定方法。方法:采用甲醇为溶剂,超声波提取,高效液相色谱法测定。色谱条件:色谱柱为C18柱,流动相为甲醇-0.2%磷酸水溶液,流速1.0 m L·min-1,柱温30℃,检测波长栀子苷为237 nm,黄芩苷为278 nm。结果:在该色谱条件下,样品中栀子苷浓度在10~100μg·m L-1(r=0.9998)及黄芩苷浓度在10~100μg·m L-1(r=0.9970)范围内二者色谱峰面积与浓度均呈良好线性关系。加样回收实验栀子苷和黄芩苷的回收率分别为97.13%和100.91%,RSD分别为2.68%和2.98%(n=6)。对3批样品的检测显示,栀子苷和黄芩苷的含量分别为6.33 mg·g-1和7.25 mg·g-1(n=3)。结论:该方法简便准确,重现性好,可用于该制剂中栀子苷、黄芩苷的质量控制。     

高效液相色谱法测定乳癖消胶囊中芍药苷的含量

目的:建立乳癖消胶囊中芍药苷的高效液相色谱测定法.方法:色谱柱为Hypersil BDS C18(4.6 mmx250 mm,5 μm),流动相为乙腈-0.1%磷酸溶液(13:87),流速为1.0 ml/min,柱温为30℃,检测波长为230 nm.结果:乳癖消胶囊中芍药苷与其他组分分离良好,且芍药苷在0.552～5.520 Ixg范围内线性关系良好(r=0.999 8,n=5),平均加样回收率为98.7%,RSD为0.8%(n=6).结论:本含量测定方法简便、准确、重现性好,可用于乳癖消胶囊的质量控制.     

HPLC测定补阳还五汤总苷中黄芪甲苷和芍药苷的含量

目的建立高效液相色谱法测定补阳还五汤总苷中黄芪甲苷和芍药苷的含量。方法色谱柱为Kromasil C18柱(4.6 mm×250 mm,5μm),黄芪甲苷测定:蒸发光散射检测器,流动相为甲醇∶水=80∶20(v/v);漂移管温度60℃;气体流量1.5 L/min;输出压力0.5 MPa;柱温30℃。芍药苷测定:流动相为甲醇∶水=23∶77(v/v);流速为1 mL/min,检测波长:218 nm,柱温:30℃。结果黄芪甲苷在0.57～9.10μg时,Y=1.506 3X+2.409 6,r=0.999 4;芍药苷在0.48~7.70μg时,Y=598.91X-2.334 6,r=0.999 9;其线性关系良好,平均加样回收率分别为99.62%和101.1%,RSD为2.6%和1.7%(n=6)。结论本法快速、简便、准确、灵敏度高,适用于黄芪甲苷及芍药苷的含量测定,并为其总苷物质制备工艺研究提供质量标准依据。     

HPLC法测定小儿退热颗粒中栀子苷的含量

目的:建立小儿退热颗粒中栀子苷的含量测定方法.方法:采用Dikmak Kromasil-C18色谱柱(4.6 mm×250 mm,5μm),流动相:甲醇-水(20:80),流速:1.0 ml/min,检测波长:238 nm,柱温35℃.结果:栀子苷进样量在0.100 5～0.502 5μg范围内,线性关系良好,平均回收率为99.05%,RSD为1.53%(n=6).结论:本方法快速、准确、重现性良好,可作为栀子苷的含量控制方法.     

不同来源栀子中栀子苷含量测定

目的 测定不同来源栀子中栀子苷含量.方法 采用HPLC法.色谱柱:Agilent C18柱;流动相:甲醇-水(25:75);柱温:30 ℃;检测波长:238nm;流速:1.0 mL/min;进样量:10μL.结果 栀子苷在2.9～57.8μg范围内呈良好的线性关系(r=0.999 5),加样回收率99.58%,RSD为2.8%.湖南石门野生栀子的栀子苷含量高;储存期短的栀子中栀子苷含量较高:相同储存期栀子栽培品和野生品栀子苷含量接近.结论 不同产地、品种、储存期栀子中栀子苷含量存在差异.     

HPLC法测定固经丸中芍药苷的含量

目的:建立固经丸中芍药苷的含量测定方法.方法:HPLC法,Kromasil C18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1%磷酸溶液(15:85),流速1 ml·min-1;检测波长230 nm.结果:芍药苷在0.1200～1.200 μg范围内,峰面积与进样量呈良好的线性关系(r=0.9998,n=6);平均加样回收率100.64%,RSD=0.94%(n=6).结论:本法快速、简便、准确、重复性好.     

复方安神胶囊中栀子苷的质量控制研究

目的:建立复方安神胶囊中栀子苷的质量控制方法。方法:采用高效液相色谱法(HPLC),色谱柱为Diamonsil C18柱(150 mm×4.6 mm,5μm);流动相为甲醇-水(15∶85);检测波长238 nm;流速1.0 m L/min;柱温30℃。结果:栀子苷进样量在0.17μg~3.4μg范围为线性关系良好,r=0.999 95,平均加样回收率为100.01%,RSD=1.60%(n=6)。结论:该方法重现性好,专属性强,结果准确,可用于复方安神胶囊中栀子苷的质量控制。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.