The therapeutic effect of Balanophora polysaccharide on acetic acid gastric ulcer in rats and its mechanism; [蛇 ? 多糖对大鼠乙酸型胃溃疡的治疗作用 ? 机制]北大核心CSCD

Aim To study the therapeutic effect of Balanophora polysaccharide(BPS)on gastric ulcer(GU)induced by acetic acid in rats and to investigateits mechanisms. Methods Sixty male SD rats were randomly divided into sham-operated group, GU model group, omeprazole positive group(3.6 mg·kg-1), and low, medium and high dose of BPS treatment groups(100, 200 and 400 mg·kg-1). The GU model group was prepared by acetic acid cautery method, and the morphology and pathological changes of ulcers were observed by visual observation combined with HE staining, and the ulcer area and inhibition rate were measured and calculated; superoxide dismutase(SOD)activity, malondialdehyde(MDA)content and glutathione peroxidase(GSH-PX)activity were measured by enzymatic assay; tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)content were detected by ELISA. The expression levels of epidermal growth factor(EGF)and epidermal growth factor receptor(EGFR)were measured by immunohistochemistry staining and Western blot. Results Compared with the sham-operated group, obvious ulcer damage was seen in the model group. Compared with the model group, the BPS-treated group showed a significant reduction in ulcer area, an increase in SOD and GSH-PX activity and EGF and EGFR expression levels, and a significant decrease in MDA, TNF-α and IL-6 content. Conclusions BPS has a therapeutic effect on GU in rats, and its mechanism may be related to the inhibition of oxidative stress, suppression of inflammatory stimuli and promotion of regenerative repair of gastric mucosa. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Influence of astragaloside on gastric mucosa of stress ulcer rats

Objective To investigate the effect of astragaloside(AST)on the gastric mucosal injury of water immersion restraint stress ulcer rat.Methods The stress ulcer model was made by water immersion and restraint.The gastric mucosal injury index was observed.The SOD activity,the MDA contents and the gene expression of melatonin receptor 1 and 2 were detected in gastric mucosa.Results Compared with the normal group,the model group showed mucous edema,hyperemia and even ulcer damage.The injury index and the MDA content of gastric mucosa in model group were significantly increased(P<0.05),the SOD activity of gastric obviously depressed(P<0.01),and the melatonin receptor 1 and 2 mRNA expressions of damaged gastric mucosa were also lower.After administration of AST,the gastric mucosal ulcer index and MDA contents relieved obviously(P<0.01,P<0.05),the SOD activity and the expressions of melatonin receptor 1 and 2 mRNA raised up(P<0.01,P<0.05).Conclusions AST could prevent the gastric mucosal damage of rat in stress ulcer.And the mechanism of the gastric mucosal protection should be concerned with regulating the melatonin receptor and lessening the injury of oxygen free radical.     

Effects of GM-CSF, IL-3, and GM-CSF／IL-3 fusion protein on apoptosis of human myeloid leukemic cell line Tf-1 induced by irradiation

AIM: To observe the effects of three cytokines on the apoptosis of Tf-1 cells induced by γ irradiation and investigate the relationship between apoptosis and caspase-3 activity. METHODS: Different cytokines GM-CSF, IL-3 and GM-CS/IL-3 fusion protein were added into the irradiated Tf-1 cells. MTT assay, morphology, flow cytometry,and DNA fragmentation assay were used to observe the effects of cytokines on apoptosis. The caspase-3 activity was determined with a fluorocytometer. RESULTS: Irradiated Tf-1 cells showed typical morphological characteristic of apoptosis demonstrated by transmission electron microscopy and were accumulated in G0/G1 phase. In the groups treated with growth factors after irradiation, three cytokines significantly increased the viability rate, distinctly decreased the apoptosis rate and the proportion of DNA fragmentation. When Tf-1 cells were irradiated by γ irradiation, caspase-3 activity was increased at different time points. In comparison with the control group in which no growth factor was added after the cells were irradiated, the caspase-3 activity of irradiated Tf-1 cells was significantly inhibited by addition of the above cytokines. Thirty-six hours after irradiation, in the control group,GM-CSF, IL-3, GM-CSF and IL-3 in combination, and two GM-CSF/IL-3 fusion protein groups, the apoptosis ratewas 73 %, 11%, 15 %, 13 %, 12 %, and 13 %. The percent of fragmented DNA was 36 %, 19 %, 18 %, 14 %,13 %, and 14 %. The fluorescence intensity was 16923, 5529, 6581, 5322, 5426, and 5485. CONCLUSION:GM-CSF, IL-3, and GM-CSF/IL-3 fusion protein could protect Tf-1 cells from apoptosis induced by γ irradiation.After Tf-1 cells were irradiated, the caspase-3 activity was significantly increased but was dramatically decreased by the above cytokines. The remarkable inhibition of caspase-3 activity may be one of the mechanisms of these hematopoietic growth factors exerting their anti-apoptotic effects.     

The protecting effects and mechanism of betaine hydrochloride on hepatic ischemia-reperfusion injury in rats

Objective To study the protecting effects and mechanism of betaine hydrochloride on hepatic ischemia-reperfusion injury in rats.Methods Fourty SD rats were randomly divided into 5 groups(8 animals in each group):sham-operated control group(A),hepatic ischemia-reperfusion group(B),200 mg·kg-1 400 mg·kg-1 800 mg·kg-1 betaine hydrochloride+hepatic ischemia-reperfusion group(C、D、E).betaine hydrochloride was administered to animals byoral route in group C、D、E for 7 days before ischemia.A、B group was administered with NS.Made the animal model of part hepatic ischemia-reperfusion.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)levels in the blood and themalondialdehyde(MDA),superoxide dismutase(SOD),protein content in hepatic tissue were determined after the liver had been reperfused for 24 hours;the hepatic tissue was examined under lightmicroscope and the cell apoptosis was demonstrated with flow cytometry.Results ALT,AST,MDA increased and SOD decreased significantly in B group when compared those in the A group(P<0.05),Hepatic apoptosis was significantly increased;ALT,AST,MDA decreased and SOD increased significantly in betaine hydrochloride 200 mg·kg-1(C)group when compared those in the B group(P<0.05).Hepatic apoptosis was significantly lower,The histologic changes of the liver tissue under lightmicroscope in the C group was more easer than in the I/R group(B).Conclusions Betaine hydrochloride has the ability to scavenge oxygen free radical(OFR),reduce lipid peroxidation and inhibition of apoptosis.So it can protect the rats liver damaged by ischemia-reperfusion.     

Gastric ulcer healing effect of wild honey and its combination with Turmeric（Curcuma domestica Val.） Rhizome on male Wistar rats

Gastric ulcer is a common disorder in human at any ages. In this research, the antiulcer activity of wild honey produced by Apis dorsata, alone or in combination with Turmeric Rhizome, was evaluated in healing acute gastric ulcer. Male Wistar albino rats（150-250 g） were induced ulcers with aspirin at 405 mg/kg BW and ethanol. Antiulcer evaluation was done based on the gastric acidity, numbers and diameter of ulcers, ulcer index, healing ratio, histological examinations, and body weight. The results showed that the groups given honey alone, turmeric alone, and combination of turmeric-honey displayed significant ulcer healing compared to the control group. Ulcers in the group administered with combination of turmeric-wild honey was different significantly from the turmeric alone and wild honey alone groups with increased body weight in that group. The result showed that wild honey（2125 mg/kg BW） had the greatest activity in healing ulcers among other groups. The combination of turmeric-wild honey had a good activity in healing ulcers and increased the body weight of the group.     

Aspirin protected against endothelial damage induced by LDL： role of endogenous NO synthase inhibitors in rats

AIM: To study the protective effect of aspirin on damages of the endothelium induced by low-density lipoprotein (LDL), and whether the protective effect of aspirin is related to reduction of nitric oxide synthase inhibitor level.METHODS: Vascular endothelial injury was induced by a single injection of native LDL (4 mg/kg) in rats. Vasodilator responses to acetylcholine (ACh) in the isolated aortic rings were determined, and serum concentrations ofasymmetric dimethylarginine (ADMA), malondialdehyde (MDA), tumour necrosis factor-α (TNF-α), and the activity of dimethylaminohydrolase (DDAH) were measured. RESULTS: A single injection of LDL (4 mg/kg)significantly decreased vasodilator responses to ACh, increased the serum level of ADMA, MDA, and TNF-α, anddecreased DDAH activity. Aspirin (30 or 100 mg/kg) markedly reduced the inhibition of vasodilator responses toACh by LDL, and the protective effect of aspirin at the lower dose was greater compared with high-dose aspiringroup. Aspirin inhibited the increased level of MDA and TNF-α induced by LDL. Aspirin at the dose of 30 mg/kg,but not at higher dose (100 mg/kg), significantly reduced the concentration of ADMA and increased the activity ofDDAH. CONCLUSION: Aspirin at the lower dose (30 mg/kg) protects the endothelium against damages elicitedby LDL in vivo, and the protective effect of aspirin on endothelium is related to reduction of ADMA concentrationby increasing DDAH activity.     

Inhibitory effect of combination of tanshinone I,metformin and aspirin on malignant melanoma model mice北大核心CSCD

OBJECTIVE: To explore the antitumor effects of combined tanshinone I (Tan I), metformin (Met) and aspirin (Asp) on malignant melanoma in mice and the possible mechanisms. METHODS: C57BL/6 mice were injected with 0.1 mL B16F10 cells (2.8×109 L-1) to establish the subcutaneous transplantation tumor model at the right forelimbs axillary. Then, the mice were divided into 8 groups according to body mass, including model group, Tan I group (20 mg.kg-1 ip), Asp group (210 mg.kg-1, orally in drinking water), Met group (70 mg.kg-1, orally in drinking water), Asp+Met group, Tan I +Asp group, Tan I +Met group and Tan I +Asp+Met group, 10 mice in each group. Each mouse drank about 7 mL of water every day for a total of 18 d. The mouse body mass was measured every other day and the tumor diameter was calculated every day. The mice were sacrificed after treatment, the tumor mass was measured and the tumor inhibitory rates were counted. The histopathological changes of the liver and spleen were observed with HE staining. The percentage of lymphocytes in the tumor tissue such as CD8T, CD4+T and Treg cells was detected by flow cytometry. Inflammatory factors such as interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α) were detected by ELISA. RESULTS: The body mass (including tumor mass) of mice in different groups increased during the experiment, but that of Tan I + Asp+Met group increased more slower than in model group (P<0.01). At the end of the experiment, no lesions were seen in any liver or spleen tissue by pathological observation, and the number of survivors was 8/10 (model group), 8/10 (Tan I group), 7/10 (Asp group), 7/10 (Met group), 8/10 (Tan I +Asp group), 8/10 (Tan I +Met group), 7/10 (Asp + Met group) and 5/10 (Tan I +Asp+ Met group), respectively. Compared with model group, there were no obvious changes in tumor volume or tumor mass in Tan I, Asp and Met groups and other two-two joint groups, but the tumor volume and tumor mass in Tan I + Asp+ Met group were significantly decreased (P<.01, P<0.05), and the tumor inhibitory rate in this group was 46.2%. Compared with the model group, the percentage of CD8+T cells increased (P<0.05) in Tan I + Asp+ Met group, but there were no significant changes in other groups. The contents of IL-6, IL-1β and TNF-α in tumor tissue of Tan I +Met group were much higher than in model group (P<0.01, P<.05, P<0.05) and the content of IL-6 increased in Tan I +Asp+Met group (P<0.01). CONCLUSION: Combination of Tan I, Asp and Met can effectively inhibit the growth of melanoma in mice, which may be related to the increasing percentage of CD8+T lymphocytes and IL-6 in tumor tissue. However there are possibly some side effects. © 2017 Chinese Journal of Pharmacology and Toxicology. All rights reserved.     

The protective effect of genistein postconditioning on hypoxia/ reoxygenation-induced injury in human gastric epithelial cells

Aim： The aim of this study was to investigate the protective effect of genistein postconditioning on hypoxia/reoxygenation- induced injury in human gastric epithelial cells and to begin a tentative discussion on the mechanism behind this protection. Methods： A model of hypoxia/reoxygenation-induced injury was established in the human gastric epithelial cell line （GES-1）. All cells in our present study were randomly divided into five groups： a normal control group （N）, a hypoxia/ reoxygenation group （H/R）, a genistein postconditioning group （GP）, a capsazepine＋genistein postconditioning group （C＋GP） and a DMSO vehicle postconditioning group （DM）. The methods used included MTT assays to test cell viability, flow cytometric analyses to quantify the percentage of cell apoptosis, Western blot analyses to measure the protein expression of calcitonin gene-related peptide （CGRP）, Bcl-2, and Bax, and immunocytochemistry assays to detect the expression of CGRP in each group. Results： The MTT assays indicated that the cell viabilities of the groups were 100.0%±0%, 51.4%±4.1%, 66.7%±2.0%, 56.1%±2.8%, and 50.7%±2.4%, respectively. Compared with the H/R group, the viability of the GP group was significantly increased （P〈0.01）. Flow cytometric analysis showed that the cell apoptosis percentage of each group was 2.28%±0.44%, 12.17%±2.15%, 5.40%±1.22%, 10.43%±1.37%, and 11.02%±2.19%, respectively. Western blot analysis demonstrated that CGRP, Bcl-2, and Bax were expressed in normal human gastric epithelial cells. Compared with the H/R group, the GP group exhibited increased expression of CGRP and Bcl-2 and decreased expression of Bax. Immunocytochemistry assays indicated that the number of CGRP-positive cells in the GP group was significantly increased. Conclusion： Genistein postconditioning has a protective effect on hypoxia/reoxygenation-induced injury in human gastric epithelial ceils. The mechanism by which genistein exerts this protection may be via activation of cell     

Therapeutic effect of Turkish galls extract on iga nephropathy model rats北大核心CSCD

OBJECTIVE: To investigate the effect of Turkish galls extract (TGE) on the expression of IgA in serum, urine and renal tissue of IgA nephropathy (IgAN) model rats. METHODS: Fifty healthy male Sprague Dawley rats were randomly divided into normal control group, IgAN model group, and TGE 75,150 and 300 mg · kg-1 groups, 10 rats per group. The model of IgAN rats was established with bovine serum albumin (BSA) + lipopolysaccharide (LPS)+carbon tetrachlorid (CCI4)for 12 weeks. From the 13th week, TGE was ig administrated once a day for 4 weeks. At the end of the 12th and 16th weeks, 24 h urine protein was measured by BCA method. At the end of the 16th week, serum and urinary IgA levels were measured by enzyme linked immunosorbent assay(ELISA), serum creatinine(SCR) and blood urea nitrogen (BUN) were detected by an automatic biochemical analyzer, and the renal pathological changes were evaluated with an Oxford classification scoring system. The deposition of IgA immune complex in the kidney was observed by immunofluorescence assay. RESULTS: At the end of 12th week, 24 h urine protein increased in all IgAN groups (P<0.05), compared with normal control group. At the end of 16th week, 24 h urine protein, IgA content in serum and urine,SCr and BUN content in serum, score in Oxford classification of renal tissue and deposition of IgA immune complex in the kidney in IgAN model group were all higher than in normal control group (P<0.05). Compared with IgAN model group, 24 h urine protein, IgA content in serum and urine and SCr content in serum were decreased in all TGE groups (P<0.05), and BUN content in serum and deposition of IgA immune complex in the kidney decreased in TGE 150 and 300 mg · kg-1 groups (P<0.05). The score in Oxford classification of renal tissue was decreased in TGE 300 mg.kg-1 group only. CONCLUSION: TGE has curative effect on IgAN model rats by reducing serum and urinary IgA and decreasing IgA immune complex deposition.     

Hepatotoxicity and anti-oxidative mechanism of mitochondrial ATPase and superoxide dismutase of Dioscorea bulbifera L. in mice北大核心CSCD

OBJECTIVE: To discuss the effect of hepatotoxicity of water extraction of Dioscorea bulbifera L. (WED) and anti-oxidative mechanism of mitochondrial adenosine triphosphate (ATP) and superoxide dismutase (SOD). METHODS: Thirty-six male ICR mice were divided into four groups: normal control (water), and WED 19.6, 28.0 and 40.0 g·kg-1 (ig) for 14 d, before the activity of serum alkaline phosphatase (ALP), glutamic-pyruvic transaminase(GPT) and glutamic-oxaloacetic transferase (GOT) was detected. The content of ATP, reactive oxygen species (ROS) and malondialdehyde (MDA) and the activity of total SOD in liver tissue of mice were detected, so were the activities of Na+- K+-ATPase, Ca2+-Mg2+-ATPase and Mn2+-SOD in mitochondria of liver tissue. RESULTS: Compared with normal control group, liver indexes of WED 28.0 and 40.0 g·kg-1 groups were increased(P<0.01). The activity of serum GPT, GOT and ALP in WED 40.0 g·kg-1 group was increased(P<0.05), but the content of ATP in WED 19.6, 28.0 and 40.0 g·kg-1 groups was decreased (P< 0.01). The content of MDA and ROS in WED 40.0 g·kg-1 group was increased (P<0.05). The activity of Na+-K+-ATPase and Ca2+-Mg2+- ATPase in WED 40.0 g·kg-1 group was decreased in mitochondria of liver tissue(P<0.05), but the activity of Mn2+-SOD was increased (P< 0.05). CONCLUSION: WED may induce liver injury and the mechanism is possibly related to mitochondria injury by weakening ATP synthesis and oxidative damage.     

Effect of human urinary kallidinogenase on cognitive function of SAMP8 mouse model and its mechanism北大核心CSCD

Aim To study the effect of human urinary kallidinogenase(HUK)on the cognitive function of SAMP8 mouse model and its mechanism. Methods SAMP8 mice were divided intofive groups:SAMP8 group,treatment group(giving 8.75×10-3,1.75×10-2,3.5×10-2,7.0×10-2 HUK),and the SAMR1 vehicle group was used as blank control. Each group was performed Morris water maze to detect spatial cognition. Afterwards the group with the most obvious cognitive improvement(HUK group)was selected for the follow-up experiments. Immunohistochemical detection of ChAT expression in CA3 area was further verified by RtPCR. Western blot was used to detect the expression of PSD95,SYN,BDNF,and pCREB protein. The activity of MPO and the content of IL-1β and IL-18 were determined. Results The passing times in the SAMP8 group was less than that of the SAMR1 group(P<0.05). The passing times of treatment group increased compared with the SAMP8 group(P<0.05 or P<0.01),and the spatial probe time of the target quadrant was shorter(P<0.05 or P<0.01). We conducted follow-up experiments with group d(HUK group). The expression of ChAT positive cells in CA3 area of SAMP8 group was significantly lower than that of SAMR1 group; the expression of positive cells in HUK group significantly increased; RtPCR showed that ChAT expression in SAMP8 group was lower than that in SAMR1 group,and ChAT expression was significantly higher than that in SAMP8 group after HUK treatment. Compared with the SAMR1 group,the levels of IL-1β,IL-18 and MPO activity in the CA3 area of SAMP8 group significantly increased,and the protein expressions of PSD95,SYN,BNDF and pCREB decreased. After HUK treatment,the content of IL-1β,IL-18 and MPO activity decreased,and the expression of PSD95,SYN,BNDF and pCREB increased. Conclusions HUK can improve the spatial cognition of SAMP8 mice. The mechanism may be achieved by promoting the expression of ChAT in CA3 area,reducing the oxidative stress and increasing synapse-related proteins. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

氟伐他汀对缺血性脑卒中患者的疗效观察及护理措施

Objective To explore the effect and nursing main points of fluvastatin on the patients with cerebral arterial thrombosis. Methods Eighty-five patients with cerebral arterial thrombosis were randomly divided into three groups. Group Ⅰ was the negative control group. Group Ⅱ was the conventional-dose therapy group and group Ⅲ was the high-dose therapy group. Each group was treated with related therapy and care. The blood lipids in all groups were tested before the treatment, 4 weeks, 12 weeks and 24 weeks after the treatment; the carotid intima-media thickness (IMT) were measured by the Germany SEQUOIAS-512-B ultra machine before the treatment and 24 weeks after the treatment; At the same time, the side effects of fluvastatin were recorded. Results The results of blood lipid tests showed that the LDL, TC and TG in two treated groups were significantly decreased compared to negative control group (P<0.05). After treated for 24 weeks, the carotid intima-media thickness (IMT) in two treated groups were significantly reduced (P<0.05); the side effects of fluvastatin were infrequent and there was no significant different among the three groups (P>0.05). Conclusion The effects of different doses of fluvastatin on cerebral arterial thrombosis were significantly different. With the dose of fluvastatin increased, the serum LDL-L and TC reduced more obvious and the inhibition on carotid atherosclerosis shows more obvious, and the side effects did not increased.     

氟伐他汀对缺血性脑卒中患者的疗效观察及护理措施

Objective To explore the effect and nursing main points of fluvastatin on the patients with cerebral arterial thrombosis. Methods Eighty-five patients with cerebral arterial thrombosis were randomly divided into three groups. Group Ⅰ was the negative control group. Group Ⅱ was the conventional-dose therapy group and group Ⅲ was the high-dose therapy group. Each group was treated with related therapy and care. The blood lipids in all groups were tested before the treatment, 4 weeks, 12 weeks and 24 weeks after the treatment; the carotid intima-media thickness (IMT) were measured by the Germany SEQUOIAS-512-B ultra machine before the treatment and 24 weeks after the treatment; At the same time, the side effects of fluvastatin were recorded. Results The results of blood lipid tests showed that the LDL, TC and TG in two treated groups were significantly decreased compared to negative control group (P<0.05). After treated for 24 weeks, the carotid intima-media thickness (IMT) in two treated groups were significantly reduced (P<0.05); the side effects of fluvastatin were infrequent and there was no significant different among the three groups (P>0.05). Conclusion The effects of different doses of fluvastatin on cerebral arterial thrombosis were significantly different. With the dose of fluvastatin increased, the serum LDL-L and TC reduced more obvious and the inhibition on carotid atherosclerosis shows more obvious, and the side effects did not increased.     

氟伐他汀对缺血性脑卒中患者的疗效观察及护理措施

Objective To explore the effect and nursing main points of fluvastatin on the patients with cerebral arterial thrombosis. Methods Eighty-five patients with cerebral arterial thrombosis were randomly divided into three groups. Group Ⅰ was the negative control group. Group Ⅱ was the conventional-dose therapy group and group Ⅲ was the high-dose therapy group. Each group was treated with related therapy and care. The blood lipids in all groups were tested before the treatment, 4 weeks, 12 weeks and 24 weeks after the treatment; the carotid intima-media thickness (IMT) were measured by the Germany SEQUOIAS-512-B ultra machine before the treatment and 24 weeks after the treatment; At the same time, the side effects of fluvastatin were recorded. Results The results of blood lipid tests showed that the LDL, TC and TG in two treated groups were significantly decreased compared to negative control group (P<0.05). After treated for 24 weeks, the carotid intima-media thickness (IMT) in two treated groups were significantly reduced (P<0.05); the side effects of fluvastatin were infrequent and there was no significant different among the three groups (P>0.05). Conclusion The effects of different doses of fluvastatin on cerebral arterial thrombosis were significantly different. With the dose of fluvastatin increased, the serum LDL-L and TC reduced more obvious and the inhibition on carotid atherosclerosis shows more obvious, and the side effects did not increased.     

氟伐他汀对缺血性脑卒中患者的疗效观察及护理措施

Objective To explore the effect and nursing main points of fluvastatin on the patients with cerebral arterial thrombosis. Methods Eighty-five patients with cerebral arterial thrombosis were randomly divided into three groups. Group Ⅰ was the negative control group. Group Ⅱ was the conventional-dose therapy group and group Ⅲ was the high-dose therapy group. Each group was treated with related therapy and care. The blood lipids in all groups were tested before the treatment, 4 weeks, 12 weeks and 24 weeks after the treatment; the carotid intima-media thickness (IMT) were measured by the Germany SEQUOIAS-512-B ultra machine before the treatment and 24 weeks after the treatment; At the same time, the side effects of fluvastatin were recorded. Results The results of blood lipid tests showed that the LDL, TC and TG in two treated groups were significantly decreased compared to negative control group (P<0.05). After treated for 24 weeks, the carotid intima-media thickness (IMT) in two treated groups were significantly reduced (P<0.05); the side effects of fluvastatin were infrequent and there was no significant different among the three groups (P>0.05). Conclusion The effects of different doses of fluvastatin on cerebral arterial thrombosis were significantly different. With the dose of fluvastatin increased, the serum LDL-L and TC reduced more obvious and the inhibition on carotid atherosclerosis shows more obvious, and the side effects did not increased.     

氟伐他汀对缺血性脑卒中患者的疗效观察及护理措施

Objective To explore the effect and nursing main points of fluvastatin on the patients with cerebral arterial thrombosis. Methods Eighty-five patients with cerebral arterial thrombosis were randomly divided into three groups. Group Ⅰ was the negative control group. Group Ⅱ was the conventional-dose therapy group and group Ⅲ was the high-dose therapy group. Each group was treated with related therapy and care. The blood lipids in all groups were tested before the treatment, 4 weeks, 12 weeks and 24 weeks after the treatment; the carotid intima-media thickness (IMT) were measured by the Germany SEQUOIAS-512-B ultra machine before the treatment and 24 weeks after the treatment; At the same time, the side effects of fluvastatin were recorded. Results The results of blood lipid tests showed that the LDL, TC and TG in two treated groups were significantly decreased compared to negative control group (P<0.05). After treated for 24 weeks, the carotid intima-media thickness (IMT) in two treated groups were significantly reduced (P<0.05); the side effects of fluvastatin were infrequent and there was no significant different among the three groups (P>0.05). Conclusion The effects of different doses of fluvastatin on cerebral arterial thrombosis were significantly different. With the dose of fluvastatin increased, the serum LDL-L and TC reduced more obvious and the inhibition on carotid atherosclerosis shows more obvious, and the side effects did not increased.     

氟伐他汀对缺血性脑卒中患者的疗效观察及护理措施

Objective To explore the effect and nursing main points of fluvastatin on the patients with cerebral arterial thrombosis. Methods Eighty-five patients with cerebral arterial thrombosis were randomly divided into three groups. Group Ⅰ was the negative control group. Group Ⅱ was the conventional-dose therapy group and group Ⅲ was the high-dose therapy group. Each group was treated with related therapy and care. The blood lipids in all groups were tested before the treatment, 4 weeks, 12 weeks and 24 weeks after the treatment; the carotid intima-media thickness (IMT) were measured by the Germany SEQUOIAS-512-B ultra machine before the treatment and 24 weeks after the treatment; At the same time, the side effects of fluvastatin were recorded. Results The results of blood lipid tests showed that the LDL, TC and TG in two treated groups were significantly decreased compared to negative control group (P<0.05). After treated for 24 weeks, the carotid intima-media thickness (IMT) in two treated groups were significantly reduced (P<0.05); the side effects of fluvastatin were infrequent and there was no significant different among the three groups (P>0.05). Conclusion The effects of different doses of fluvastatin on cerebral arterial thrombosis were significantly different. With the dose of fluvastatin increased, the serum LDL-L and TC reduced more obvious and the inhibition on carotid atherosclerosis shows more obvious, and the side effects did not increased.     

氟伐他汀对缺血性脑卒中患者的疗效观察及护理措施

Objective To explore the effect and nursing main points of fluvastatin on the patients with cerebral arterial thrombosis. Methods Eighty-five patients with cerebral arterial thrombosis were randomly divided into three groups. Group Ⅰ was the negative control group. Group Ⅱ was the conventional-dose therapy group and group Ⅲ was the high-dose therapy group. Each group was treated with related therapy and care. The blood lipids in all groups were tested before the treatment, 4 weeks, 12 weeks and 24 weeks after the treatment; the carotid intima-media thickness (IMT) were measured by the Germany SEQUOIAS-512-B ultra machine before the treatment and 24 weeks after the treatment; At the same time, the side effects of fluvastatin were recorded. Results The results of blood lipid tests showed that the LDL, TC and TG in two treated groups were significantly decreased compared to negative control group (P<0.05). After treated for 24 weeks, the carotid intima-media thickness (IMT) in two treated groups were significantly reduced (P<0.05); the side effects of fluvastatin were infrequent and there was no significant different among the three groups (P>0.05). Conclusion The effects of different doses of fluvastatin on cerebral arterial thrombosis were significantly different. With the dose of fluvastatin increased, the serum LDL-L and TC reduced more obvious and the inhibition on carotid atherosclerosis shows more obvious, and the side effects did not increased.     

番茄红素对寰枢椎失稳老龄小鼠记忆的影响

Objective To observe the effect of Lycopene on the improvement of memory abilities by cumulating lactic acid for long time in atlas dentata vertebrae senile mice. Methods Totally 30% lactic acid 30μL one time every week was injected into the place between the first and the second cervical vertebrae for 3 weeks. Forty mice were randomized into five groups:young control group, model group, VitE group(50 mg/kg), control group,and two therapeutic groups of lycopene which was intragastric at the doses of 5, 2.5 mg/kg once everyday. The changes of memory in mice were observed with water-maze test and step-down avoidance test. The activities of acetylcholine esterase(AchE), and the content of acetylcholine, (Ach) in encephalon were tested. HE staining in mice brain, including the cortex and hippocampus was demonstrated. Results Compared with model group. Lycopene high dose could significantly shortened the latency period and wrong times in water-maze test (P ＜0.01 ), In stepdown avoidance test, the reaction period was shortened significantly ( P ＜ 0.01 ) and the latency period was shortened significantly. The activities of ChE decreased (P ＜0.01 ) and the content of ACh increased in the Lycopene high doses group(P＜0.01 ). The therapeutic groups of lycopene had less pathological change of cellular necrosis,neuron loss in hippocampal CAI than the model group. Conclusion Lycopene has a certain protective and improving effect on the decline of memory ability and cervical lesion induced by lactic acid in atlanto-axial joint cumulating.