胃癌患者EB病毒感染与Cyclin D1基因表达的相关性

目的:研究胃癌患者癌组织中EB病毒(EBV)感染与Cyclin D1表达的关系. 方法:原位杂交法检测胃癌患者石蜡标本中EBV编码的小RNA(EBER1);免疫组化法检测EBV阳性胃癌[EBV( )] 及EBV阴性胃癌[EBV(-)]标本细胞周期蛋白Cyclin D1的表达状况. 结果:胃癌患者癌组织标本217例中23例EBER1( ); EBV( )组Cyclin D1蛋白表达显著高于EBV(-)组(P＜0.01). 结论:EBV感染可能上调Cyclin D1表达.     

EBV相关胃癌组织中bcl-2和Ki-67蛋白表达的检测

①目的　探讨Epstein BarrVirus(EBV)相关胃癌 (EBVaGC)组织中bcl 2和Ki 6 7蛋白表达与EBV感染的关系。②方法　应用PCR Southern技术 ,检测 1 85例胃癌及相应癌旁组织中EBVDNA ,原位杂交技术检测PCR阳性标本EBV小RNA1 (EBER1 )的表达 ,确证EBVaGC。并选择EBV阴性胃癌 (EBVnGC)标本 ,应用免疫组化技术检测其bcl 2和Ki 6 7蛋白的表达。③结果　 1 85例胃癌组织中检测到EBVaGC 1 3例 ,阳性率为 7.0 3% ,1 85例癌旁组织均为阴性 ,胃癌组织和相应癌旁组织EBV阳性率比较差异有显著性 (χ2 =1 1 .0 77,P <0 .0 0 1 )。E BVaGC和EBVnGC组织中bcl 2表达率分别为 5 3.85 %和 4 8.89% ,二者比较差异无显著性 (χ2 =0 .0 99,P >0 .0 5 )。EBVaGC和EBVnGC组织中Ki 6 7指数 (KI)平均值分别为 2 8.2 5 4± 6 .2 84和 37.86 4± 1 4 .5 2 3,二者比较差异有极显著意义 (t′=2 .5 5 3,P <0 .0 1 )。④结论　EBV感染与部分胃癌的发生有一定相关性 ,EBVaGC的发生与bcl 2蛋白表达无明显相关性 ,与Ki 6 7蛋白表达有一定相关性。     

胃癌变过程中Hp感染与凋亡基因Bcl-2表达相关性研究

目的 研究胃癌变过程中幽门螺杆菌(Hp)感染与凋亡基因Bcl-2表达、细胞凋亡和临床意义.方法 用快速尿素酶法、W-S银染法和美蓝法联合检测62例慢性浅表性胃炎(CSG)、55例慢性萎缩性胃炎(CAG)、52例肠化生(IM)、46例不典型增生(AH)、65例胃癌(GC)组织中Hp的感染,并采用免疫组化及TUNEL法分别检测Bcl-2蛋白的表达及细胞凋亡水平.结果 Hp感染率、Bcl-2蛋白阳性表达率均随着胃癌形成中病变恶性程度的加重而明显上升.Hp感染率CAG、IM、AH和AC组较CSG,GC组较CAG组均明显升高 (P＜0.05);IM、AH、GC组阳性表达率明显高于CSG组(P＜0.01,P＜0.05);GC组的阳性表达率明显高于IM组(P＜0.01);AH、GC组Hp阳性患者Bcl-2蛋白表达率均高于Hp阴性患者(P＜0.05);IM、AH、GC组中Bcl-2阳性者凋亡指数均低于Bcl-2阴性者;Hp感染阳性胃癌患者组织中Bcl-2蛋白表达与组织分化程度有明显关系,低未分化胃癌Bcl-2蛋白表达明显高于高中分化胃癌(P＜0.01).结论 在胃黏膜癌变过程中,Hp感染可能通过逐渐上调Bcl-2基因的表达,抑制细胞凋亡和分化发挥致癌作用.     

Hp感染与胃癌及癌前病变中Bcl-2、p16蛋白表达关系的研究

目的 :研究幽门螺杆菌 (Helicobacterpylori,Hp)感染 ,胃癌及癌前病变患者抑癌基因 p16和关键性凋亡调节基因Bcl- 2蛋白的表达 ,探讨Hp可能的致癌机制。方法 :应用免疫组化方法测定 86例Bcl- 2、p16蛋白表达情况 ,其中 2 0例胃癌组织、18例异型增生、12例肠上皮化生、2 4例萎缩性胃炎、12例浅表性胃炎 ,用快速尿素酶法和HE染色检测Hp感染情况。结果 :胃癌组Hp感染 12例 (12 / 2 0 ,6 0 % )。Bcl- 2在异型增生组织中的阳性表达率与胃癌、肠上皮化生相比无显著性差异 (P >0 0 5 )。Hp阳性组肠上皮化生、异型增生组织中Bcl- 2的阳性表达率显著高于Hp阴性组 (P <0 0 5 )。p16在慢性胃炎中阳性表达率显著高于胃癌、肠上皮化生和异型增生 (P <0 0 5 )。Hp阳性萎缩性胃炎组织的p16阳性表达率低于Hp阴性组 (P <0 0 5 )。结论 :在胃癌发生的早期即存在p16基因表达低下 ,Bcl- 2蛋白表达增加 ,Hp可能作为促癌剂在胃癌的发生发展中起一定作用。     

胃癌患者EBV感染潜伏基因产物表达的研究

目的:研究EBV阳性胃癌中潜伏基因产物EBV编码的小RNA(EBER1)及潜伏膜蛋白LM P1的表达状况。方法:原位杂交法检测胃癌患者石蜡包埋组织标本中EBER1;免疫组化法检测EBV阳性胃癌中LM P1。结果:217例胃癌患者癌组织标本中检测到23例EBER 1;EBV阳性胃癌中无潜伏感染膜蛋白LM P1表达。结论:EBV阳性胃癌中缺乏潜伏感染膜蛋白LM P1的表达。     

胃癌组织中hMSH2蛋白表达与幽门螺杆菌感染的关系

目的本研究通过检测幽门螺杆菌(HP)感染和非感染胃癌组织中错配修复基因hMSH2蛋白的表达,探讨hMSH2基因在胃癌发生中的作用和HP致癌的可能机制。方法采用W S法对49例胃癌患者癌组织中HP感染情况进行检测;利用免疫组化方法检测49例胃癌患者癌组织中hMSH2的表达情况。结果29例HP感染阳性胃癌组织中,hMSH2基因蛋白表达阳性率(62.07%);20例HP感染阴性的胃癌中,hMSH2基因蛋白表达阳性率(100.00%),HP感染阳性胃癌组hMSH2基因蛋白表达阳性率与HP感染阴性组统计学分析有显著性差异。(P<0.05)结论hMSH2基因蛋白表达异常与胃癌发生有关;HP感染引起hMSH2基因蛋白表达阳性率降低可能是导致胃粘膜癌变的分子机制之一。     

胃癌和相应癌旁组织中EB病毒感染的检测

①目的 探讨Epstein—BarrVirus(EBV)感染与胃癌发生发展的关系。②方法 应用PCR—Southern检测172例胃癌组织和相应癌旁组织中EBVDNA，阳性标本进一步用原位杂交技术检测EBV小RNA(EBERl)的表达，以确证EBV感染。③结果 172例胃癌组织11例EBV阳性，阳性率为6．39％，EBV阳性率与病人性别、年龄、组织学分型和临床分型之间无明显关系(P=0．069～0．485)；172例相应癌旁组织中未检测到EBV基因组的存在，胃癌组织及相应癌旁组织EBV阳性率差异有显著意义(P=0．003)。④结论 部分胃癌组织可检测到EBV基因组，EBV感染与胃癌的发生有一定的相关性。     

胃癌FHIT基因异常及与EB病毒感染相关性的研究

目的:检测胃癌FHIT基因转录、Fhit蛋白表达以及EB病毒(EBV)的感染情况,探讨EBV感染对FHIT基因异常表达的影响及其与胃癌发生的关系.方法:应用巢式RT-PCR法检测30例胃癌组织和30例配对正常胃黏膜组织中FHIT基因的表达,并对异常转录本进行测序;Western杂交法检测10例胃癌Fhit蛋白的表达;PCR法检测50例胃癌(包括RT-PCR检测30例)EBV的感染情况.结果:正常胃黏膜组织中均检测到正常大小转录本,11例(36.7%)胃癌检测到异常转录本;测序结果1例异常转录本缺失FHIT外显子5～7,1例缺失FHIT外显子4～7;4例(40.0%)Fhit蛋白表达缺失或降低;5例(10.0%)EBV阳性,其中4例(80.0%)同时存在FHIT基因的异常转录本.结论:FHIT基因及其产物Fhit蛋白的缺失在胃癌的发生发展中起重要的作用.EBV感染可能通过引起FHIT基因的异常导致胃黏膜上皮细胞癌变.     

HP相关性胃癌组织中COX-2和Bcl-2的表达及其意义

目的:探讨HP相关性胃癌组织中COX-2和Bcl-2的表达及其意义。方法:选取68例胃癌组织为实验对象,各对应的癌旁组织为对照组,并选取20例正常胃组织为正常对照组。检测各样本的COX-2和Bcl-2蛋白,并对所得数据进行统计分析。结果:COX-2及Bcl-2蛋白在胃癌组织、癌旁组织及正常组织中的阳性表达率分别为61.8%和70.6%,25.0%和27.9%,15.0%和30.0%。COX-2及Bcl-2蛋白在胃癌组织中的阳性表达率均明显高于癌旁组织及正常组织。胃癌组织中COX-2与Bcl-2表达显著正相关。HP感染的胃癌组织中COX-2及Bcl-2的表达明显高于无HP感染的胃癌组织。结论:胃癌组织中,HP的感染率较高,HP相关性胃癌中,COX-2与Bcl-2均高度表达且密切相关。HP感染可能通过COX-2的过度表达,诱导细胞增殖并刺激Bcl-2蛋白表达抑制细胞凋亡,从而使细胞增殖和凋亡失衡,促进胃癌的发生发展。     

幽门螺杆菌感染与胃癌及癌前病变中PCNA和Bcl-2表达关系的研究

目的:研究幽门螺杆菌(Helicobacter pylori,H.pylori)感染对胃癌及癌前病变患者增殖细胞核抗原(PCNA)和关键性凋亡调节基因Bcl-2蛋白表达的影响,探讨H.pylori可能的致癌机制.方法:应用免疫组化方法测定20例胃癌组织、18例重度异型增生、12例肠上皮化生、24例萎缩性胃炎、16例浅表性胃炎组织中Bcl-2、PCNA蛋白表达情况,快速尿素酶法和HE染色检测H.pylori感染情况.结果:胃癌组H.pylori感染12例,与慢性浅表性胃炎相比差异有显著性(P＜0.05).从浅表性胃炎到胃癌PCNA指数呈递增趋势,各组间差异均有显著性(P＜0.05).浅表性胃炎和萎缩性胃炎组中H.pylori阳性组与H.pyloir阴性组的PCNA LI差异有显著性(P＜0.05),其余各组间H.pylori阳性和H.pylori阴性病例间的PCNA LI差异无显著性.Bcl-2蛋白在异型增生组中的阳性表达率与胃癌、肠上皮化生相比差异无显著性,与慢性胃炎相比差异有显著性.H.pylori阳性组肠上皮化生、异型增生组织中Bcl-2阳性表达率显著高于H.pylort阴性组,它们之间差异有显著性(P＜0.05).结论:H.pylori感染可促使PCNA、Bcl-2蛋白表达增加,H.pylori可能作为促癌剂在胃癌的发生发展中起一定作用.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.