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1.
本文作者研究了上皮生长因子受体(EGFR)和C-erbB-2癌基因蛋白在50例甲状腺癌和66例甲状腺良性疾患中的表达。50例甲状腺癌中,EGFR阳性者26例(52%),C-erbB-2阳性者25例(50%)。EGFR和c-erbB-2共同表达阳性者的淋巴结转移率(92.85%)明显高于两者共同表达阴性者(61.53%)(P<0.05)。EGFR阳性者AgNOR数(2.59±0.64/核)也明显高于EGFR阴性者(1.39±0.18/核)(P<0.001)。EGFR与C-erbB-2共同表达阳性,同时AgNOR计数高的甲状腺癌具有较高的恶性度。因此,对这种病人要予以特别的治疗措施并抓紧随访工作。 相似文献
2.
ER,EGFR,p53在乳腺癌组织中的比较研究 总被引:2,自引:0,他引:2
采用免疫组化染色技术,检测75例原发乳腺癌组织中雌激素受体(ER),表皮生长因子受体(EGFR)及p53的表达。结果表明:ER、EGFR、p53的阳性表达率分别为49.3%、41.3%和37.3%。ER、EGFR的表达与腋淋巴结转移、临床分期有明显相关性(P<0.05);与年龄及肿瘤大小无明显相关性(P>0.05)。EGFR与ER有负相关性(P<0.05)。在有4个以上腋淋巴结转移的21例病例中,EGFR阳性者14例,占66.7%。20例临床Ⅲ期的病例,EGFR阳性者15例,占75.0%。p53的表达与腋淋巴结转移、临床分期、年龄及肿瘤大小均无明显相关性(P>0.05);与ER呈负相关性(P<0.05);与EGFR呈正相关性(P<0.05)。研究认为EGFR蛋白表达阳性的乳腺癌病人预后不良,p53蛋白表达阳性与乳腺癌病人的预后无明显相关性。 相似文献
3.
表皮生长因子及其受体在胃癌患者中的表达 总被引:1,自引:0,他引:1
采用放射免疫法测定26例胃癌病人血清、尿液EGF水平,同时采用免疫组化SABC法检测胃粘膜组织切片中EGFR染色的阳性率,并与正常对照进行比较分析。结果表明:胃癌患者血清和尿EGF水平均显著高于正常对照(3.72±1.83μg/L与1.77±0.60μg/L,P<0.01;18.44±17.88ng/mg与5.19±6.37ng/mg,P<0.01),胃粘膜组织切片EGFR染色阳性率也明显高于正常对照(73%与11%,P<0.01)。胃癌病人中,EGFR染色阳性者血清EGF水平显著高于EGFR阴性者(P<0.05),但两者尿EGF水平无显著性差异。胃癌患者血清、尿EGF水平与胃癌大小、分化程度及淋巴结转移无显著性相关。 相似文献
4.
表皮生长因子及其受体在慢性萎缩性胃炎中的表达和致癌变意义的探讨 总被引:10,自引:0,他引:10
采用放射免疫法测定慢性萎缩性胃炎患者(50例)血清、尿中表皮生长因子(EGF)水平,同时应用免疫组化SABC法检测胃粘膜组织切片中表皮生长因子受体(EGFR)染色的阳性率,并与正常人及胃癌患者作对照分析。结果表明:慢性萎缩性胃炎组血清、尿EGF水平均显著高于正常对照组(P<0.01),但与胃癌组间差异无显著性意义(P>0.05)。胃粘膜组织切片EGFR染色阳性率的统计分析结果与体液EGF一致。慢性萎缩性胃炎病人血清EGF水平与胃粘膜EGFR表达有同步性;伴有Ⅱb型肠化者的血清EGF水平较其他类型肠化者有更高水平表达(P<0.05)。中度与重度慢性萎缩性胃炎间血清、尿EGF水平与胃粘膜组织切片中EGFR染色阳性率差异均无显著意义 相似文献
5.
利用雄性SD大鼠每日经口给予有机锗(Ge-132)100、250、500mg/kg连续二十五d,发现高剂量组的肝细胞色素P450受到明显抑制(P<0.05)。对P448的标志酶乙氧基异吩口恶唑脱乙氧基酶(EROD)的抑制也近50%;动物以Ge-132500mg/kg/d预先处理20d,再分别给予苯巴比妥钠盐(PB)、3-甲基胆蒽(3-MC),发现Ge-132对PB诱导P450的抑制不明显,而对3-MC诱导EROD的抑制仍达33%。 相似文献
6.
用免疫组化ABC方法,检测胃癌和正常胃粘膜中上皮生长因子(EGF)及其受体(EGFR)、雌激素受体(ER)的表达。结果:64例胃癌中,EGF阳性39例(60.9%),EGFR阳性33例(51.6%),ER阳性12例(18.8%);58例正常胃组织中,EGF、EGFR阳性各3例,ER阴性。癌与正常对照组间差异有显著性(P<0.05)。提示,EGF、EGFR、ER与胃癌有关。胃癌EGF、EGFR的阳性率又非常显著高于ER(P<0.005),说明EGF、EGFR在胃癌发展中的调节作用远较雌激素重要。结果还提示,EGF、EGFR、ER的相互关系影响胃癌细胞的发生、种植和转移。 相似文献
7.
通过对86例乳腺癌的FCM分析:发现二倍体肿瘤24例,异倍体62例。前者SPF16.8±10.3%,后者SPF22.2±9.8%(P<0.05)。其中进行ER检查的64例中发现:ER阳性组异倍体占66%,ER阴性组异倍体占92.9%(P<0.05);前者SPF18.1±9.1%,后者SPF26.0±13.0%(P<0.05)。CEA阳性与否与异倍体检出率、SPF及ER阳性率无明显关系。月经状态、腋淋巴结转移与FCM检测结果无关系。因此作者认为FCM分析可了解肿瘤本身的生物学特性,并可成为新的、独立的预后指标。 相似文献
8.
以ABC法检测了40例妊娠性滋养细胞肿瘤石蜡标本P185及ER、PR的表达。其中绒毛膜癌24例。侵蚀性葡萄胎16例,P185阳性率为40%,ER、PR阳性率分别为45%,75%,P185在侵蚀性葡萄胎中的阳性率均明显高于绒瘤(P<0.01)。病程小于1年者,P185的阳性率高于病程大于1年者(P<0.05),ER、PR阳性者,P185的阳性率分别高于其阴性者(P<0.05、P<0.01),P185阳性与阴性的患者,分别有81.3%、50%的患者于3疗程内血hCG转阴(P<0.05)。资料提示:P185倾向于在滋养细胞肿瘤恶性转化的早期表达,P18S阳性者对化疗较为敏感。 相似文献
9.
癌症患者血液高粘状态对肿瘤血管内和远处转移的作用 总被引:9,自引:0,他引:9
对96例恶性肿瘤患者与100例正常人进行血液流变学和纤维蛋白检测,同时对52例恶性肿瘤血管内和远处转移患者与44例非转移患者进行血液流变学和纤维蛋白原的对比研究。结果:恶性肿瘤组ηb(80S-1)、ηb(20S-1)、ηP、ESR、K值、ET、EM、RE、R-ηb(80S-1)、R-ηb(20S-1)和Fib均显著高于正常人组(P<0.05);HCT两组间无明显差异(P>0.05)。癌症转移组ηb(80S-1)、ηb(20S-1)、ηP、HCT、ET、RE、R-ηb(80S-1)、R-ηb(20S-1)和Fib均显著高于非转移组(P<0.05);ESR、K值、EM两组间无显著差异(P>0.05)。认为:血液流变性增高与恶性肿瘤有直接关系,当肿瘤转移时血液流变性和纤维蛋白原增高则更为显著。 相似文献
10.
桂皮酸对PGCL3人肺癌细胞增殖和核仁组成区的影响 总被引:4,自引:0,他引:4
本研究的目的是探讨低毒性的食品调味剂桂皮酸对PGCL3人肺癌细胞的抑制作用。研究结果发现1mmol/L和3mmol/L桂皮酸能使PGCL3人肺癌细胞增殖受到明显抑制(P<0.05,P<0.01),增殖抑制率达48.6%,同时发现细胞的核仁形成率明显下降,每个细胞平均核仁数由2.92个降至1.66和1.96个(P<0.01),核仁内核仁组成区嗜银蛋白(AgNOR)颗粒明显减少,每个细胞核仁内AgNOR颗粒数由39.26±7.49降至9.54±4.61和19.68±10.48(P<0.001和P<0.01)。表明桂皮酸是PGCL3人肺癌细胞有效的抑制剂,在抗癌作用方面具有潜在的应用价值。 相似文献
11.
Objective To study the antineoplastic effect of the calcium channel blocker verapamil and 5-fluorouracil intraperitoneal chemotherapy
on hepatocarcinoma-bearing rats, and examine the action between calcium channel blockers and cytotoxic drugs.
Methods We adopted the method of subcapsular implantation of carcinoma tissues of walker-256 in the left liver lobe as a model of
liver carcinoma-bearing rats. All experimental animals were divided into four groups. On the sixth day post implantation,
in group A (control group) 6 ml of saline was injected intraperitoneally once a day for 3 days. In group B (single chemotherapy
group) 6 ml, of 5-Fu 75 mg/kg was injected intraperitoneally once a day for 3 days. In group C (combination of treatment group)
both 5-Fu (75 mg/kg) and verapamil (25 mg/kg) were administered simultaneously as in A and B. In group D (simple verapamil
group) only 6 ml of verapamil (25 mg/kg) was administered as above.
Results Compared with groups A, B and D, The volume of cancer and the contents of liver cancer DNA and protein were significantly
reduced. The rates of inhibiting cancer (89.9% in group C and 35.4% in group B) were significantly increased in group C. Group
C had significantly long survival time compared to groups A, B and D (P<0.05). By light microscopy, a number of focal necroses were found in cancer tissue in group C.
Conclusion Calcium channel blockers can enhance the antineoplastic effect of 5-Fu intraperitoneal chemotherapy to liver cancer: The use
of verapamil can not increase the toxicity of 5-Fu. 相似文献
12.
13.
近交系F344大鼠喉移植改良模型的建立 总被引:4,自引:1,他引:3
背景与目的:喉为非生命必需器官,喉移植研究一直滞后于其它生命必需器官的移植,然而,一旦宿主对移植喉的免疫耐受诱导成功,那么,喉移植将是晚期喉癌治疗和喉功能重建的重要手段。本研究的目的在于建立改良鼠喉移植模型,提高受体鼠和移植喉的存活率,为喉移植抗免疫排斥和免疫耐受诱导研究提供动物模型。方法:采用近交系F344大白鼠建立喉移植Strome模型,并对该模型进行改良:供体喉切取时保留甲状腺上动脉和咽升动脉,形成舌骨、舌根、下咽、喉、甲状腺、部分颈段食管、气管的移植复合体,移植喉血运通过供体鼠双侧颈总动脉分别与受体鼠颈总动脉和颈前静脉端-端吻合重建,术中行对侧颈外静脉穿刺补液,术后皮下注射补液。术后1周解剖移植喉,观察移植喉大体形态和血管通畅情况,常规病理检查评价移植喉的组织变化和存活状况,对比Strome模型和改良模型的受体鼠及移植喉存活率。结果:StromeA组、StromeB组、改良组的受体鼠存活率和各组移植喉存活率分别为70%(14/20)、85%(17/20)、95%(19/20)和30%(6/20)、40%(8/20)、80%(16/20),改良模型优于Strome模型。结论:与Strome模型比较,改良模型加强围手术期的处理,降低受体鼠的死亡率;将咽升动脉及其分支包含在移植复合体中,增加移植血管蒂中喉供血动脉的侧支循环,降低侧支循环阻力,减少微循环障碍的发生率,提高移植喉的存活率。 相似文献
14.
Danilkov AP Kirpatovskiĭ VI Kudriavtsev IuV Golovanov SA Ivashchenko VV Salmanov SA Drozhzheva VV Boĭko TA 《Urologii?a (Moscow, Russia : 1999)》2002,(3):29-33
The opportunity of raising kidney structural and functional tolerance to ischemia with parenteral injections of 0.06% solution of sodium hypochlorite for 4 days in preischemia period was studied in experiment on 22 non-inbred rats of 200-280 g body weight. 90 minute ischemia was created by ligating the left and right kidney arteries, veins, ureters. Morphological and functional kidney data, enzymuria activity of nephron epithelium cells were registered, 80 and 33% of the rats survived 7 days after ischemia in the study and control groups, respectively. The control animals showed deterioration of the tubules function, high enzymuria. Thus, it is possible to raise kidney tolerance to ischemia by preischemic parenteral injection of sodium hypochlorite solution. 相似文献
15.
Parity in humans and rats provides significant protection against mammary tumor development. This study was carried out to investigate whether treatment of parous rats with mammotropic hormones would affect methyl-nitrosourea (MNU)-induced mammary carcinogenesis. Parous rats were treated with 17beta-estradiol (E2), progesterone (P4) and thyroxine (T4) alone or in combination. E2 (20 microg/60 days) and P4 (20 mg/60 days) were administered by silastic tubing and T4 in the drinking water (3 microg T4/ml). Hormonal treatments commenced 7 days before MNU injection and continued for 33 weeks. Animals were palpated weekly for tumor detection. The effects of the hormonal treatments on the circulating concentrations of E2, P4, growth hormone (GH), prolactin (PRL), T4 and insulin-like growth factor-I (IGF-I) after 7 days of treatment, the time of MNU injection, was assessed. Animals treated with E2 had significantly elevated circulation concentrations of GH, PRL and P4, and serum levels of E2 were more consistent in this group than in the other animal groups. P4 treatment caused elevation in P4 concentration in serum but did not affect the circulating levels of other hormones. The proliferation of the mammary gland at the time of MNU injection was elevated in animal groups treated with E2 either alone or with P4 and T4 and in animals treated with P4 alone, but the mammary gland was most differentiated in untreated parous rats and least in animals treated with E2 either alone or with P4 and T4. Mammary tumor incidence was 10% in parous rats that did not receive any hormonal treatment. Treatments with E2 or P4 alone significantly increased the susceptibility of parous animals to 67 and 50.0%, respectively; a tumor incidence similar to that of untreated AMV rats (64%). Parous rats treated with E2 plus P4 had tumor incidence higher than 90%. T4 administered did not affect mammary carcinogenesis. 相似文献
16.
Yehia El-Hennawi G Yancey Gillespiel Mahesh A Varian Pam Watkins M S Mahaley Jr Darell D Bigner 《Journal of neuro-oncology》1987,4(3):309-313
We have examined the impact of methylprednisolone acetate (MPA) on survival of F344 rats that were bearing avian sarcoma virus (ASV)-induced gliomas and that were treated optimally with radiotherapy. Toxicity of MPA (dose range of 0.2–5.0 mg/kg × 7 over 3 weeks) was first established in non-tumor bearing rats as assessed by their relative failure to gain weight. Doses of 2.0 or 5.0 mg/kg x 7 caused animals to be 21.8 or 43.9%, respectively, underweight compared with vehicle controls. In rats bearing ASV induced gliomas, treatment with 3 000 cGY (nine fractions over a 3-week period) alone or with 0.2 or 1.0 mg MPA/kg (× 6 during the 3-week radiotherapy course) produced a significantly prolonged survival compared with that of untreated, tumor bearing rats. However, MPA did not enhance survival when given concurrently with radiotherapy; indeed, at the higher of these two doses, median survival of tumor-bearers was slightly less than with radiotherapy alone. This trend towards interference with the beneficial effects of radiotherapy was more pronounced with the highest dose of MPA studied, 5.0 mg/kg body weight × 6. These animals had a median survival time that was significantly less than that of tumor-bearers receiving radiotherapy alone, but not significantly different from untreated rats with gliomas. The possible significance of these observations is discussed. 相似文献
17.
Rephaeli A Blank-Porat D Tarasenko N Entin-Meer M Levovich I Cutts SM Phillips DR Malik Z Nudelman A 《International journal of cancer. Journal international du cancer》2005,116(2):226-235
AN-7, a prodrug of butyric acid, induced histone hyperacetylation and differentiation and inhibited proliferation of human prostate 22Rv1 cancer cells in vitro and in vivo. In nude mice implanted with these cells, 50 mg/kg AN-7 given orally thrice a week led to inhibition of tumor growth and metastasis, tumor regression in >25% of animals and increased survival. Median time to the experimental end point (tumor volume 2 cm3 or death) in the untreated was 52 days, and average tumor volume was 0.8 +/- 0.18 cm3. At the same time, 94.4% of AN-7-treated mice survived and had average tumor volumes of 0.37 +/- 0.1 cm3. PSA expression was a useful marker for 22Rv1 lung metastasis detection. Sizeable metastases positively stained for PSA and limited air gaps were found in lungs of untreated mice. In animals treated with AN-7, lung morphology appeared normal. Primary tumors of treated animals were highly positive for PSA and had an elevated level of p21 and the proapoptotic protein Bax. Sections taken from AN-7-treated animals, examined under an electron microscope, exhibited condensed chromatin and apoptotic bodies. PSA serum levels were higher in untreated compared to treated animals and correlated with tumor volume. Since prolonged oral administration with 50 mg/kg or a single oral dose of 1.2 g/kg AN-7 did not cause adverse effects and the former exhibited significant anticancer activity, AN-7 is likely to display a high therapeutic index and may be beneficial for prostate cancer patients. 相似文献
18.
M I Díaz Gómez H M Godoy A Marzi E C de Ferreyra O M de Fenos J A Castro 《Journal of the National Cancer Institute》1981,67(5):1089-1092
Prior administration of Triton WR-1339 (tyloxapol, an anionic surfactant) to noninbred Sprague-Dawley male rats significantly enhanced the intensity of the necrogenic effect of dimethylnitrosamine (DMN) on the liver. This phenomenon was established by determination of NADP+-linked isocitrate dehydrogenase activity in the plasma and by histologic procedures. This enhancing effect was not due to an increase in the levels of DMN that reached the liver, because the content of DMN in the livers of Triton WR-1339-treated or untreated animals at 1 or 3 hours was not significantly different. Triton WR-1339 administration had no effect on DMN liver metabolism to formaldehyde or CO2; in addition, the covalent binding of DMN metabolites to nucleic acids or proteins was not modified by pretreatment with Triton WR-1339. However, in vitro, high concentrations (1 mg/ml) of Triton WR-1339 decreased the intensity of these parameters. This effect disappeared when the concentration was lowered to 0.4 mg/ml. Results are compatible with the hypothesis that the potentiating effects of Triton WR-1339 on liver damage caused by DMN and other hepatotoxins were due to a modification of the response of liver cells to injury. 相似文献
19.
Ross Donald A. Kish Phillip Muraszko Karin M. Blaivas Mila Strawderman Myla 《Journal of neuro-oncology》1998,40(1):29-38
It is our hypothesis that low grade gliomas are the glial counterparts of other precancerous lesions such as colon polyps and, therefore, suitable targets for chemoprevention. Steps in the molecular progression of gliomas have been described, indicating that an accumulation of abnormalities is required for progression to a high grade and interruption of this progression might be possible. An animal model of chemical glial carcinogenesis was used to test this hypothesis. Pregnant rats were injected intravenously with ENU (ethylnitrosourea) on the 18th day of gestation to induce gliomas in the offspring, which were randomized to receive control diet, diet supplemented with vitamin A palmitate, or diet supplemented with N-acetylcysteine. Animals exposed to ENU and receiving a control diet developed brain tumors and had a shortened life expectancy compared with rats unexposed to ENU. The animals treated with NAC showed no statistically significant delay in the time to tumor and no change in the histologic grade of the tumors when compared with animals receiving control diet, but the time to death from any cause of NAC treated animals differed significantly from untreated animals. Animals receiving high dose VA had statistically significantly prolonged time to tumor, survived significantly longer than untreated animals, but had no reduction in the total number of tumors or change in the histologic grade of their tumors. The theoretical basis of these results is likely due to the putative mechanism of action of these agents. These data indicate that glioma chemoprevention is possible and deserves further exploration. 相似文献
20.
K Ohashi H Hara F Takahashi R Aso T Akimoto K Nakama 《Gan to kagaku ryoho. Cancer & chemotherapy》1992,19(11):1825-1829
Menogaril (TUT-7) is a novel antitumor antibiotic belonging to anthracyclines. The pharmacokinetic parameters derived from plasma concentration-time profiles after repeated (for 14 days) or single oral administration of TUT-7 to rats were found to be not significantly different by either administration schedule. The rats with artificial liver dysfunction were obtained by subcutaneous application of carbon tetrachloride (CCl4, 1 ml/kg) for 3 days. After oral administration of TUT-7 to the rats with CCl4-induced liver toxicity (3 daily administrations of 1mg/kg, S.C.), the maximum plasma concentrations (Cmax) and AUC of both the unchanged drug and its metabolite N-Demethyl menogaril, were increased. Also over all elimination was slower in animals with liver dysfunction. 相似文献