化学发光及硝基蓝四唑法检测癣病患者中性粒细胞的功能研究

本文应用全血鲁米诺依顿化学发光法(CL)检测36例皮肤丝状菌感染患者的粒细胞吞噬功能,并与硝基蓝四唑试验(NBT)对照。发现患者嗜中性粒细胞的CL峰值,峰时与积分值均明显高于正常组,且敏感性及特异性优于NBT。     

男性非淋菌性尿道炎586例病原体感染检测分析

随着性病流行病谱的改变,非淋菌性尿道炎(NGU)发病呈上升趋势,为探讨男性NGU病原体感染状况,对我院586例NGU患者中的衣原体(CT)和解脲支原体(UU)检测情况进行分析,现将结果报告如下。1材料与方法1.1检测对象对性病就诊者取尿道分泌物作革兰氏染色镜检,在油镜下查多形核白细胞(PMNL)数,平均视野≥3个的在排除淋病后为CT和UU检测对象。586例均为首诊者,年龄19岁~55岁之间。1.2PMNL量化分段根据在油镜(1000倍)视野下多形核白细胞数>4个为阳性[1],将PMNL量化分三段:3~4个( ),5~14个( ),≥15个( )。1.3检测方法CT检测采用英国UN…     

昆虫、寄生虫性皮肤病

20 0 2 2 86 4　我国虫媒性皮肤病及其治疗 (综述 ) /周吉礼 (山东滨州医学院寄生虫教研室 )∥皮肤病与性病 .-2 0 0 2 ,2 4 (1) .- 16～ 18螨类引起的皮肤病有疥疮、蠕形螨 (酒渣、毛囊炎、痤疮、脂溢性皮炎 )、恙螨性皮炎及革螨性皮炎、蜱性皮炎、虱蚤性皮炎、毒毛虫性皮炎、皮肤蝇蛆病、啮虫性皮炎及其他虫咬性皮炎等。参 16　 (张孝友 )2 0 0 2 2 86 5　徐闻县人体蠕形螨感染情况调查 /周大森(广东徐闻县卫防站计划免疫防疫科 )…∥中华流行病学杂志 .- 2 0 0 2 ,2 3(1) .- 31共调查 2 178人 ,蠕形螨感染 711例 ,感染率为32 .6 4%。以毛囊蠕形螨感染为主 (占 92 .0 4 % ) ;男性感染率为 34.4 1% (42 7/ 12 4 1) ,女性为 30 .31% (2 84 /937) ;以 2 6～ 4 5岁组感染率最高 (38.76 % ) ;教师偏高为 4 1.90 % ;以鼻唇沟和颊部为主要感染部位 ;酒渣鼻的蠕形螨感染率为 95 .0 0 % ,可见酒渣鼻与蠕形螨感染有一定关系 ,且蠕形螨感染者大多数有面部痒...     

病毒性皮肤病患者外周血淋巴细胞亚群的检测分析北大核心CSCD

目的探讨病毒性皮肤病患者的免疫功能状态。方法应用流式细胞仪分析检测486例病毒性皮肤病患者外周血中的淋巴细胞亚群,并与正常人进行比较。结果病毒性皮肤病患者之间外周血淋巴细胞亚群比较,CD4^+T细胞,CD8^+T细胞,CD3^-CD19^+细胞,CD3^-CD16^+56^+NK细胞,CD4^+/CD8^+差异有统计学意义(P<0.05)。单纯疱疹患者组与带状疱疹患者组比较,CD8^+T细胞,CD3^-CD16^+56^+NK细胞,CD4^+/CD8^+差异有统计学意义(P<0.05)。寻常疣患者组、扁平疣患者组与尖锐湿疣患者组三组相比较,CD4^+T细胞、CD8^+T细胞、CD3^-CD19^+细胞、CD3^-CD16^+56^+NK细胞差异也有统计学意义(P<0.05)。与正常组相比较,五种病毒性皮肤病患者组CD3^+T细胞、CD4^+T细胞、CD8^+T、CD4^+/CD8^+细胞除带状疱疹患者组,CD3^-CD19^+细胞仅尖锐湿疣患者组,CD3^-CD16^+56^+NK细胞除尖锐湿疣患者组外差异均有统计学意义(P<0.05)。结论各类病毒性皮肤病患者均存在不同程度的免疫异常。免疫功能的紊乱是病毒性皮肤病患者发病的一个重要机制,在各类病毒性皮肤病患者的发生、发展过程中可能起到重要的作用。     

病毒性皮肤病患者外周血淋巴细胞亚群的检测分析

目的探讨病毒性皮肤病患者的免疫功能状态。方法应用流式细胞仪分析检测486例病毒性皮肤病患者外周血中的淋巴细胞亚群,并与正常人进行比较。结果病毒性皮肤病患者之间外周血淋巴细胞亚群比较,CD4~+T细胞,CD8~+T细胞,CD3~-CD19~+细胞,CD3~-CD16~+56~+NK细胞,CD4~+/CD8~+差异有统计学意义(P0.05)。单纯疱疹患者组与带状疱疹患者组比较,CD8~+T细胞,CD3~-CD16~+56~+NK细胞,CD4~+/CD8~+差异有统计学意义(P0.05)。寻常疣患者组、扁平疣患者组与尖锐湿疣患者组三组相比较,CD4~+T细胞、CD8~+T细胞、CD3~-CD19~+细胞、CD3~-CD16~+56~+NK细胞差异也有统计学意义(P0.05)。与正常组相比较,五种病毒性皮肤病患者组CD3~+T细胞、CD4~+T细胞、CD8~+T、CD4~+/CD8~+细胞除带状疱疹患者组,CD3~-CD19~+细胞仅尖锐湿疣患者组,CD3~-CD16~+56~+NK细胞除尖锐湿疣患者组外差异均有统计学意义(P0.05)。结论各类病毒性皮肤病患者均存在不同程度的免疫异常。免疫功能的紊乱是病毒性皮肤病患者发病的一个重要机制,在各类病毒性皮肤病患者的发生、发展过程中可能起到重要的作用。     

麻风病的吞噬作用:瘤型麻风血循环中白细胞的粘附和细胞摄粒能力的缺陷

作者研究了28例活动性瘤型麻风成人患者和18名正常成人对照者的外周血多形核白细胞(PMN)和单一核细胞(MN)的细胞摄粒作用(endocytosis),即观察从瘤型患者及正常人外周血分离的这两类白细胞在正常人或瘤型麻风患者的血清中吞噬鼠麻风分枝杆菌的能力.结果:瘤型患者的MN和PMN在正常人血清中孵育,或在瘤型的血清中孵     

大疱及疱疹性皮肤病

20130850大疱性类天疱疮外周血淋巴细胞亚群分析/莫小辉(上海市皮肤病医院),谈亦,谭飞…∥中国麻风皮肤病杂志.-2012,28(12).-844～846应用流式细胞仪检测22例大疱性类天疱疮(BP)患者外周血CD3+,CD3+CD4+,CD3+CD8+,CD3-CD16+CD56+和CD19+淋巴细胞亚群的百分比,并应用FlowCount荧光球进行绝对计数。结果 :BP患者CD3+和CD3+CD8+百分率与对照组差异有统计学意     

皮肤血管病及淋巴管病

20101640 50例过敏性紫癜变应原生物共振检测结果分析/乔丽(新疆自治区中医院皮肤科),马壮年∥皮肤病与性病.-2010,32(1).-30~31应用生物共振技术检测50例过敏性紫癜患者过敏原。结果,过敏性紫癜患者过敏原阳性依次为环境化学毒素、菌菇、动物皮毛、海鲜类、花粉、螨类、食品添加剂。认为吸入类及食物过敏原诱导的变态反应是过敏性紫癜的重要发病因素之一,有效避免接触过敏原,有利于该病的恢复并可以减少复发。表1参2(马宏恩)20101641二乙酰氨乙酸乙二胺联合红花注射液治疗腹型过敏性紫癜疗效观察/耿瑛(云南玉溪市医院)∥皮肤病与性病.-2010,32(1).-29~3054例腹型过敏     

氯苯吩嗪增强掌蹠脓疱病的吞噬作用

据以往报告在掌蹠脓疱病(PPP)以及其它一些皮肤病中,经体外试验发现其嗜中性白细胞的吞噬能力具有缺陷。近来Brandt证实氯苯吩嗪(Clofazimine)可能增强嗜中性白细胞的吞噬活性。作者对27例(男性8例,女性19例)PPP病人口服氯苯吩嗪治疗的临床效果及其对吞噬作用的影响进行了研究,观察时间均在一年以上。这些病人的年龄为27～63岁,平均为45岁。病期均在3年以上,至少在氯苯吩嗪治疗前的一年来病情处于静止期,即既无自然缓解也不因治疗而缓解。其中8例有     

面部皮脂溢出性皮炎及激素依赖性皮炎与蠕形螨的相关性

目的探讨蠕形螨感染与面部皮肤病的相关性。方法使用纯刮法检查200例面部皮肤病患者（研究组）,同时检查143例无面部皮损的其他皮肤病人（对照组）,比较两组感染情况的差异。结果200例面部皮肤病人蠕形螨感染率59．5％远高于对照组的19．58％（28／143）,（P〈0．01）。两组均显示蠕形螨感染无性别差异,随着年龄的增大,蠕形螨感染率相应增加。纯刮法检查蠕形螨感染的阳性率以颊部最高（45．5％）,其次为鼻部（26％）。结论蠕形螨感染与面部皮肤病的发生有一定关系,在面部皮肤病治疗时可配合进行抗蠕形螨的治疗。     

Measuring human skin buffering capacity: an in vitro model

Background/purpose: It has been thought that skin possesses buffering capacity. This study measured the skin buffering capacity against two model solutions of acid and base at three concentrations with an in vitro system. Methods: Ten microliters of model base (sodium hydroxide – NaOH) and acid (hydrochloric acid – HCl) solutions at concentrations of 0.025, 0.05, and 0.1 N was applied to human cadaver skin (3.18 μL/cm²) placed onto glass diffusion cells. Phosphate‐buffered saline (PBS) was used as a standard buffer solution. Deionized water served as the negative control, whereas untreated skin served as the blank control. Skin pH was read and recorded immediately following dosing (0 time), and at 10 and 30 min of post‐dosing. After the 30 min of dosing, each skin, except untreated skin (blank control), was then washed by applying 1 cm³ of deionized water. The pH on each washed skin was measured immediately following washing, and the pH measurement was repeated at 10 and 30 min of post‐washing. Six replicates were conducted. Results: The pH values sharply significantly increased (P<0.05) immediately following dosing with NaOH at all concentrations (the highest concentration, caused the highest pH), and then decreased closely to baselines within 30 min post‐application but still remained at significantly (P<0.05) higher values when compared with the blank control (untreated skin). HCl (acid) significantly (P<0.05) decreased skin pH immediately following dosing with all concentrations (the highest concentration, caused the lowest pH) and then restored rapidly to baseline. There was no significant difference in post‐washing procedures on the skins that were pre‐treated with the acid (HCI) solutions. However, with all base solutions (NaOH) pre‐treated skin, pH values were significantly higher (P<0.05) at all time points post‐washing. Furthermore, both PBS and water controls significantly elevated (P<0.05) the pH values following washing. Conclusion: Skin pH and its buffering capacity can be measured on human cadaver skin in vitro, which may partially replicate the response of in vivo skin. Dose–response was noted; i.e. the higher concentration caused larger changes in skin pH. In addition, the restoration of skin pH is relatively faster with acid when compared with base treatment. Clinical implications are offered.     

获得性反应性穿通性胶原病九例临床及病理分析

对2015年1月至2017年12月我院9例获得性反应性穿通性胶原病临床资料和组织病理进行回顾性分析。其中男女比例为1:1.25,平均发病年龄53.89岁,平均病程9.67个月,临床皮损形态多样,组织病理均可见变性的胶原纤维穿出表皮,有8例患者（88.9％）伴有糖尿病,2例患者（25%）伴有高血压。9例患者均被误诊为其他疾病,最易误诊为结节性痒疹 和湿疹或湿疹样皮炎 ,其余依次是皮肤血管炎 、坏疽性脓皮病 、皮肤溃疡、真菌感染、神经性皮炎、痒疹性大疱性类天疱疮、大疱性表皮松解症、扁平苔藓,均为1例次。     

Mycological findings in andrological patients. Statistical study in the years 1971-1981

In the years 1971 to 1981, clinical signs of mycotic skin infection were found in 589 andrological patients (2.7%). Microscopic proof and absence of positive microscopic results, respectively, are not sufficient for diagnosis; therefore, the culture is a conditio sine qua non for exact diagnosis. A positive mycotic culture was found in 13% of the cases with a negative microscopic investigation.     

Polymorphonuclear leukocyte chemotaxis in generalized pustular psoriasis

We studied polymorphonuclear leukocyte (PMNL) chemotaxis in 3 patients with generalized pustular psoriasis using 3 chemoattractants: Zymosan-activated serum from both the patients and normal individuals, and n-formyl-methionyl-leucyl-phenylalanine (FMLP). These attractants were assayed against both patient-derived and normal PMNLs. All patients had quiescent skin disease at the time of assay. We found that all patients had decreased PMNL chemotaxis to autologous Zymosan-activated serum, but not to FMLP. PMNLs from normal individuals responded normally to serum from the patients. We conclude that patients with generalized pustular psoriasis, when free of symptoms, may have an isolated PMNL chemotactic defect that is restricted to serum-derived attractants.     

Effect of infection with Trichophyton mentagrophytes varietas interdigitale on phagocytosis in humans

BACKGROUND AND AIM: Phagocytosis by polymorphonuclear leucocytes (PMNLs) and macrophages is important in the defence of human organisms, especially in mycotic infections of the skin. The aim of this study was to examine the relationship between phagocytosis and a chronic type of infection with Trichophyton mentagrophytes varietas interdigitale (T. m. var. interdig.). MATERIALS AND METHODS: A group of 256 patients was investigated from 1990 to 2000. They were all treated with terbinafine. The parameters for phagocytosis were analysed by the Hersy method. RESULTS: The immunological status of all of the patients was altered. Ingestion, digestion and random mobility decreased significantly (P < 0.001). Out of 256 patients treated with terbinafine, 196 (76%) were cured completely and the values for phagocytosis became normal. CONCLUSION: This investigation confirms the defect in the function of phagocytes of patients chronically infected by T. m. var. interdig. Terbinafine was shown to be an effective antimycotic drug, both fungicidal and immunostimulative.     

单纯疱疹病毒2型DNA疫苗对豚鼠抗病毒感染的研究

目的 探讨单纯疱疹病毒2型DNA疫苗的抗病毒感染效应.方法 重组DNA疫苗pcgD免疫雌性豚鼠,采用血清中和实验检测抗体,单纯疱疹病毒2型(HSV-2)sav株接种豚鼠,观察DNA疫苗保护动物抵抗HSV-2病毒感染的能力.结果 pc-gD免疫豚鼠产生的中和抗体效价远远高于对照组和生理盐水组,感染病毒后,表现出初发感染推迟,复发感染的严重程度下降.结论 DNA疫苗能有效地保护动物免受HSV-2病毒的攻击,延缓初发感染,减少复发感染.     

Polymorph count for predicting non-gonococcal urethral infection: a model using Chlamydia trachomatis diagnosed by ligase chain reaction

BACKGROUND/OBJECTIVES: The criteria for the diagnosis of non-gonococcal urethritis (NGU) on a Gram stained urethral smear are derived from previous studies which used culture as a diagnostic test for Chlamydia trachomatis. Our objectives were (1). to re-assess the relation between urethral polymorph count and C trachomatis infection, using ligase chain reaction (LCR) as the diagnostic test; and (2). to assess other possible predictors of C trachomatis infection such as symptoms, signs, demographic and behavioural variables. METHODS: We collected data from 363 men consecutively attending a genitourinary medicine clinic (excluding those with gonorrhoea and follow up visits) who had a urethral smear and a urethral LCR test for C trachomatis. The sensitivity and specificity of a discrete cut off in urethral polymorphonuclear leucocyte (PMNL) count as a diagnostic test for chlamydia urethritis were calculated. The associations between other variables, such as age and symptoms, and this infection were also estimated. RESULTS: 8% of men had C trachomatis infection and 26% of men had a PMNL count of 5 or more. Of those men with chlamydia 37% did not have NGU; 20% of men with NGU had chlamydia. Adjusted odds ratios for risk of chlamydial infection were significant for age less than 30 relative to 40 years and over (adj OR 13.6; 95% confidence interval 1.69 to 110), a PMNL count of 20 or more (6.56; 2.15 to 20.0), a PMNL count of 5-19 (3.59; 1.41 to 9.15), and the symptom of dysuria (3.27; 1.32 to 8.08). However a PMNL count of 5 or more was only 63% sensitive and 77% specific for C trachomatis infection. No association between sexual behaviour and chlamydial infection was found in this setting. CONCLUSIONS: The PMNL count is associated with presence of chlamydial infection but a large proportion of men with chlamydia have PMNL counts below the recommended cut off for a diagnosis of NSU. Lower age and the presence of symptoms may be as predictive as the urethral polymorph count for chlamydial urethritis and possibly for other urethral infections.     

B超引导下穿刺治疗剖宫产术后子宫切口感染的疗效分析

目的:探讨剖宫产术后子宫切口感染患者采用B超引导下穿刺进行治疗的疗效,为临床有效治疗术后切口感染提供依据。方法:选择2013年8月至2015年8月在医院进行剖宫产,并且术后发生切口感染的产妇共56例。试验组产妇选择B超引导下穿刺手段进行治疗,对照组产妇常规治疗。结果:试验组产妇中无效及总有效情况分别为2例(7.14%)、26例(92.86%),对照组产妇中无效及总有效情况分别为7例(25.00%)、21例(75.00%),试验组产妇中总有效率比对照组高,其差异有统计学意义(P0.05)。试验组产妇中拆线时间、体温高峰及住院时间平均值分别为(7.02±0.71)d、(36.63±0.28)℃、(4.01±2.93)d,对照组产妇中拆线时间、体温高峰及住院时间平均值分别为(9.82±0.93)d、(37.72±0.69)℃、(9.53±1.49)d,差异均有统计学意义(P0.05)。试验组产妇中硬结及红肿渗液情况分别为2例(7.14%)、6例(21.43%),试验组产妇中硬结及红肿渗液情况分别为12例(42.86%)、23例(82.14%),差异均有统计学意义(P0.05)。结论:诱发剖宫产患者术后出现切口感染的临床危险因素多种多样,临床上采用B超引导下穿刺技术进行治疗时,可使产妇切口感染情况得到显著改善,有利于产妇的康复,在临床上值得重视。     

Impairment of some microphage functions in recurrent herpes simplex

Chemotactic, phagocytic and intracellular killing activities of polymorphonuclear leukocytes (PMNL) were investigated in vitro in 49 patients suffering from recurrent bouts of herpes simplex. A slight impairment of chemotactic activity and a markedly reduced capacity of killing Candida albicans blastospores were revealed. Phagocytic capacity and NADH-dependent oxidase activity of PMNL proved to be normal. The results point to an alteration of some PMNL functions in these patients.