氯化铈对T淋巴细胞集落形成的影响

目的　研究体外加氯化铈 (CeCl3 )对T淋巴细胞集落形成的影响。方法　采用T淋巴细胞集落微量甲基纤维素法测试CeCl3 对T淋巴祖细胞的影响及FCM检测T淋巴细胞集落细胞凋亡的变化。结果　T淋巴祖细胞的生长和凋亡与CeCl3 呈浓度依赖关系 ,CeCl3 在 1.2 5× 10 -7M～ 1.2 5× 10 -6M时T淋巴细胞集落增长近一倍 ,此时的凋亡率为 3.0 3% ,在 1.2 5× 10 -3 M开始出现抑制作用 ,随着浓度的增高直至没有集落形成 ,在 1.2 5× 10 -2 M凋亡率高达 11.33%。结论　低剂量的Ce3 + 能促进T淋巴祖细胞的生长 ,在 1.2 5× 10 -7M～ 1.2 5× 10 -6M时达最佳浓度 ;随着浓度的增高 ,在 1.2 5× 10 -3 M开始出现抑制T淋巴细胞集落细胞的生长 ,促进凋亡。     

急性髓系白血病细胞P170表达与白血病集落生长关系的研究

为研究急性髓系白血病 (AML)细胞 P170表达与白血病细胞体外集落生长之间的关系及其预后意义 ,对 31例 AML 住院患者骨髓细胞用 Dicke法进行 AML 白血病集落 (AML- CFU)体外培养 ,并用单抗免疫组化法对骨髓细胞和 AML- CFU细胞 P170表达进行检测。 2 8例 AML 患者采用蒽环类药 Ara- c治疗 ,2个疗程后评定疗效。结果显示 :初发、复发 AML、慢粒急粒变及 CR期 AML 患者 AML- CFU集落分别为 172 .9± 72 .1/ 2×10 5 ,176 .0± 76 .6 / 2× 10 5 ,2 2 4.5± 5 4.7/ 2× 10 5和 9.7± 5 .0 / 2× 10 5 ,初、复发 AML 及 CML 急粒变患者 AML-CFU明显高于 CR期 AML 患者 (P<0 .0 1) ,而初、复发 AML 及慢粒急粒变患者 AML- CFU无差异。 AML 患者AML- CFU细胞和 BM细胞 P170阳性表达和 P170阴性表达均为 16例。两种细胞 P170阳性表达一致者 13例 ,P170阴性表达一致者 12例 ,P170阳性和 P170阴性表达不一致者各 3例 (P=0 .114)。 P170阴性者 CR10例 (10 /13,76 .9% ) ,P170阳性者 11例 CR2例 (2 / 11,18.2 % ) ,P=0 .0 0 48。多元回归及逐步回归分析 AML 患者 CR率与P170表达及疾病状态有关 ,其相关系数分别为 - 0 .9786和 - 0 .40 5 5 (P值分别为 0 .0 0 94和 0 .0 10 5 )。以上可见 ,AML- CFU数量与疾病活跃     

再生障碍性贫血(再障)患淋巴细胞亚群的分析

一些证据表明,免疫机理可能再与再障患者造血祖细胞的破坏或抑制。当从患者血中除去T—淋巴细胞后,可增加造血细胞集落形成。已证明再障患者血及骨髓中存在这种对造血细胞集落有抑制作用的淋巴细胞。作者测定了28例再障患者血中的T淋巴细胞。亚群辅助T细胞/诱导T细胞(OKT4+)与抑制T细胞/细胞毒T细胞(OKT7+)的平均比值,比值为1.24±0.74X±SD,明显低于正常人(1.78±0.57)和其它血液病患者(1.82±0.92)。53％的患者由于辅助T细胞/诱导T细胞对数减少。而抑制T细胞/细胞毒T细胞增加,比值倒置(≤1)。12例再障患者中7例HLA-DR显示增     

Rhodaminel23介导的光动力学疗法对小鼠淋巴细胞增殖及骨髓干祖细胞集落形成的影响

目的　探讨Rhodamine12 3(Rh12 3)介导的光动力学疗法对小鼠淋巴细胞增殖及骨髓干祖细胞集落形成的影响。方法　以C5 7B/ 6小鼠为供鼠 ,BALB/c小鼠为受鼠 ,脾脏淋巴细胞混合培养 ,比较单纯Rh12 3、单纯激光照射、光动力学疗法对小鼠混合淋巴细胞增殖 (MTT法 )及骨髓粒单核巨噬细胞集落形成 (CFU -GM)的影响 (甲基纤维素培养基法 ) ;流式细胞仪检测混合淋巴细胞中CD3 CD6 9 阳性率。结果　Rh12 3组及单纯激光组对细胞增殖无显著影响 ,光动力学组 2 0mW/cm2 以下剂量激光对细胞增殖无明显影响 ,30mW/cm2 以上明显抑制细胞增殖 ;30mW/cm2 以下剂量对CFU -GM集落无明显影响。光动力学疗法后细胞培养 2 4h ,CD3 CD6 9 表达明显下降。结论　光动力学疗法在 30mW/cm2 激光照射时可抑制淋巴细胞增殖 ,降低早期活化T细胞表达 ,但不影响骨髓CFU -GM集落形成     

全反式维甲酸对骨髓纤维细胞集落形成的影响

本文主要通过纤维细胞集落的培养,观察了急性早幼粒细胞性白血病的骨髓纤维细胞集落,结果急性早幼粒细胞白血病的骨髓纤维细胞集落形成单位(CFU-F)较正常者明显低下4.3±4.9/2×~510细胞(n=9);完全缓解后,则上升为12.4±5.7/2×10~5(n=5)正常水平。体外试验结果显示维甲酸对 CFU-F 具有抑制作用。加入维甲酸之后,CFU-F 则降为3.11±3.5/2×10~5细胞,P<0.05。维甲酸诱导急性早幼粒细胞性白血病,外周血白细胞总数先出现高峰,然后进入低谷,后者可能与白血病细胞浸润有关外,还可能与骨髓纤维细胞被抑制有关.     

Serum granulocyte colony-stimulating factor in patients with chronic renal failure

目的　探讨粒系集落刺激因子 (G CSF)的调节机制及代谢途径。方法　本文运用酶联免疫吸附试验 (ELISA)检测 61例慢性肾功能衰竭 (CRF) / 血液透析患者血清G CSF水平。结果　CRF患者血清G CSF水平明显升高。CRF非血透患者血清G CSF检出率为 80 65% ,血清G CSF水平为566 4 0± 2 0 7 98ng/L。CRF血透患者G CSF检出率为 93 33% ,血透前和血透后G CSF水平分别为 12 55 36±611 2 5ng/L和 1151 61± 599 4 7ng/L。血透组G CSF水平略高于非血透组 ,但两组无明显差异。血透前与血透后患者G CSF水平无明显差异。进一步分析表明 ,G CSF水平与WBC ,BUN或Scr水平无关 (P >0 0 5)。结论　CRF患者血清G CSF水平升高可能是由于肾功能不全导致G CSF清除减少或 /和G CSF产生增加。     

人脐血基质细胞深低温保存的实验研究

目的　探讨一种简便可行的人脐血基质细胞冻存方法。方法　采用 5 %二甲基亚砜 (DMSO) ,3%羟乙基淀粉 (HES)和 4 %人血白蛋白 (HAS)作冷冻保护剂 - 80℃冻存人脐血基质细胞 ,观察不同时相点的基质细胞活性、细胞计数 ,回收率 ,基质细胞集落形成单位 (CFU F)及其支持集落生长的作用 (CFU S ,CFU GM ,CFU E)。结果　保存 6个月后人脐血基质细胞的细胞活性仍保持在 (90 .6± 2 .8) % ,CFU F产率也达到 (83.6± 1 .8) % ,其支持集落生长的作用与保存前无统计学差异。结论　本方法是一种简便易行的人脐血基质细胞保存法     

慢性肾功能衰竭患者IL—2活性与T淋巴细胞亚群之间的关系

慢性肾功能衰竭(CFR)病人存在细胞免疫功能紊乱。随着细胞免疫及白细胞介素—2(IL—2)在免疫调节的不断认识,其与CRF的关系日益受到重视。本文测定38例CRF病人外周血T淋巴细胞亚群和IL—2活性,探讨它们之间的关系。一、材料与方法:38例CRF病人,男26例,女14例,年龄42±6.8岁;正常对照组30例,男18例,女12例,年龄44±5.2岁。 T淋巴细胞亚群测定:肝素抗凝血,PRMI—1640稀释,2000r/rain离心20分钟,吸出淋巴细胞,用5％小牛血清的1640配成4×10~5/ml,与McAb-A-E试剂混匀,放置10分钟,500r/min离心5分钟,用0.8％戊二     

系统性红斑狼疮血小板减少患者巨核祖细胞分化障碍

目的　明确系统性红斑狼疮 (SLE)血小板减少患者骨髓巨核祖细胞增殖分化情况 ,以及患者血清对巨核祖细胞增殖分化的影响。方法　分离 7例SLE血小板减少患者 (SLE血小板减少组 )、11例胸外科开胸手术切除肋骨患者 (正常对照组 )和 4例SLE血小板正常但病情活动患者 (SLE血小板正常组 )的骨髓单个核细胞 ,体外巨核祖细胞 (CFU MK)无血清半固体培养 ;并分别加入SLE血小板减少和SLE血小板正常患者的血清 ,观察血清对CFU MK集落形成的影响。结果　SLE血小板减少组CFU MK集落数为 (2 7.33± 9.30 )个 /片 ,明显低于正常对照组的 (6 1.2 2± 2 9.71)个 /片和SLE血小板正常组的 (6 0 .0 0± 2 9.71)个 /片 (P值均 <0 .0 5 ) ;加入SLE血小板减少患者血清后 ,SLE血小板减少组 (n =7)和正常对照组 (n =7)CFU MK集落数分别减少为(14 .2 9± 6 .73)和 (2 9.4 4± 2 3.35 )个 /片 (P <0 .0 5 )。加入SLE血小板正常患者血清后 ,正常对照组 (n =7)CFU MK增加到 (115 .6 0± 72 .99)个 /片 ,与未加血清者的差异无显著性 (P <0 .0 5 ) ;而SLE血小板减少组 (n =7)无显著增加 (P >0 .0 5 )。结论　SLE血小板减少患者骨髓巨核祖细胞分化障碍 ,患者血清对骨髓巨核祖细胞的分化具有抑制作用     

甲基纤维素和羟乙基淀粉分离脐血有核细胞的比较

目的 :为寻找一种较好的脐血有核细胞分离法。方法 :分别采用甲基纤维素和羟乙基淀粉对 3 5例脐血进行分离 ,并以有核细胞的回收率 ,细胞活度 ,CD+34 细胞百分率及脐血干 /祖细胞体外培养下形成CFU GM ,BFU E和CFU Meg 3种祖细胞集落数对这 2种方法进行比较。结果 :有核细胞回收率的细胞活度 ,CD+34 细胞回收率的甲基纤维素法分别为 89% ,97% ,7 6%± 2 % ;羟乙基淀粉法分别为 80 5 % ,91% ,5 0 %± 1 6%。干细胞体外培养下形成CFU GM ,BFU E ,CFU Meg集落数的甲基纤维素法分别为 92 5± 5 ,14 5± 19,2 9± 8;羟乙基淀粉法分别为 80± 4 ,12 7±18,2 1± 5。结论 :甲基纤维素分离法具有有核细胞回收率高 ,祖细胞含量丰富 ,增殖能力强的优点     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.