Comparative Study on Immunologic Effect of Composite Prescriptions of Tonifying Kidney, Invigorating Spleen and Promoting Blood Circulation Drugs in Mice

Ｃｏｍｐａｒａｔｉｖｅ　Ｓｔｕｄｙ　ｏｎ　Ｉｍｍｕｎｏｌｏｇｉｃ　Ｅｆｆｅｃｔ　ｏｆ　Ｃｏｍｐｏｓｉｔｅ　Ｐｒｅｓｃｒｉｐｔｉｏｎｓ　ｏｆ　Ｔｏｎｉｆｙｉｎｇ　Ｋｉｄｎｅｙ,　Ｉｎｖｉｇｏｒａｔｉｎｇ　Ｓｐｌｅｅｎ　ａｎｄ　Ｐｒｏｍｏｔｉｎｇ　Ｂｌｏ...     

Clinical and Experimental study on treatment of fatty liver of Phlegm-Dampness Stagnation type by Zhiganle capsule

Objective: To explore the mechanism of Zhiganle capsule ( , ZGLC) in treating fatty liver of Phlegm-Dampness Stagnation type. Methods: One hundred and forty-six patients were treated with ZGLC, the effects were observed and compared with those treated with Dongbao Gantai(DBGT, ) tablets as control. The effect of ZGLC on ethionine induced hyperlipemia model rats was also observed. Results: The effect of ZGLC was obviously better than those of DBGT in relieving or removing symptoms, normalizing the ultrasonic abnormal findings, lowering the serum levels of lipid and enzyme and improving hemorrheologic functions. Experimental study showed that ZGLC could reduce the blood lipids, inhibit the lipid deposition in liver. As compared with the model group, level of triglyceride in the ZGLC treatment group were much lowered (P < 0.01) and the effect was better than those in the control group. Conclusions: ZGLC has the effect of reducing blood triglyceride level, inhibiting lipid deposition in liver and improving hemorrheologic function.     

Changes of Blood Flow in the extremities in patients with rheumatoid arthritis and treatment with integrated traditional and Western medicine

Blood flow of the extremities was measured in 33 cases of rheumatoid arthritis and 30 of healthy persons by personal vascular laboratory. It was found that the values of extremity rheography in the patients were significantly lower than that in the controls. Treated with blood-circulation-promoting and stasis-removing traditional Chinese medicine and Mailuoning Injection which has a beneficial impact on anti-coagulation, removing embolus and activating circulation, the symptoms, signs and the blood flow in the extremities of the patients with rheumatoid arthritis were markedly improved.     

Treatment of immunological infertility with Chinese medicinal herbs of ziyin jianghuo

According to the microcosmic Syndrome Differentiation, immunological infertility is recognised as Kidney Yin Deficiency-Hyperactivity of Fire Syndrome. Zhibai Dihuang Pills , a recipe of Chinese medicinal herbs which plays a nourishing Yin to remove Fire action, was used to treat the infertile couples with antisperm and/or antizona pellucida antibodies in their blood serum. It was found that after the treatment, the antibodies were converted to negativeness in 81.3% of 32 immunological infertile couples. All 8 cases of successful pregnancy occurred in 1～9 months after negative conversion of the antibodies and the negative status was maintained throughout the course of pregnancy.     

Clinical and experimental study of the effect of Xilixin Granule combined with radiotherapy on malignant tumorsGranule combined with radiotherapy on malignant tumors

Objective: To observe the effect of Xilixin Granule combined with radiotherapy in treating malignant tumors.Methods: Thirty six patients of pulmonary or nasopharyngeal carcinoma were treated with Xilixin granule (XLXG) combined with radiotherapy and compared their efficacy with that of 31 patients treated by Zhenqi Fuzheng granule combined with radiotherapy for control.Results: The symptoms of Yin Deficiency syndrome in the treated group were obviously improved, the leucocyte decreased by 5.6%, while in the control group it reached 25. 8%, the 3 year survival rate was significantly higher in the treated group (75. 0%) than that in the control group (51.6%). Animal experiment revealed that XLXG had the effects of tumor inhibition, it could increase white blood cells, platelets and hemoglobin of patients, especially in using large dosage.Conclusion: XLXG have some protective effect against radiotherapeutic damage in patients with malignant tumor.     

Clinical study on therapeutic effect of shuwei decoction on functional dyspepsia

Objective

To explore the clinical effectiveness and therapeutic mechanism of Shuwei Decoction in treating functional dyspepsia (FD).

Methods

A total of 124 FD patients were randomly divided into two groups: Shuwei Decoction group (60 cases) and the control group (64 cases) treated with cisapride. After one month treatment, all patients were examined with motilin and electrogastrography (EGG).

Results

The therapeutic effectiveness of Shuwei Decoction group was much better than that of the control group (P<0. 05), while the changes on motilin and EGG in the two groups were relatively similar (P<0.05).

Conclusion

Shuwei Decoction could effectively raise motilin level, promote gastric movement and improve the clinical symptoms in FD patients.     

Treatment of integrated traditional and western medicine in recurrent spontaneous abortion of immune abnormality type

RecurrentsPOntaneousabortion(RSA),previouslynamedhabitualabortionisacommonobstetricalcomplication.Mostcasesisidiopathic,exceptthatafewcasesareduetochromosomalaberrations,luteinic.dysfunc..non,genitaltractabnormalitiesandsoon(1).Wi.ththedevelopmentofreproductiveimmunology,reseachersaroundtheworldhavedevotedtostudyonimmuneetiology,diagnosisandtreatmentofRSA,whichhasresultsinremarkableprogressintreatingthestubbornandcomplicatedill.ess(2).Accordingtoinvestigationofimmuno--epidemiologyandimmunee…     

Clinical study in treating fallopian tube obstruction with catheter recanalization and blood stasis removing herbal drugs

The 100 patients with fallopian tube obstruction were firstly treated by catheter recanalization, and then randomly divided into two groups: Chinese medicine group (CMG) was treated with Tongjingbao and Angelicae Complex Injection, and Western medicine group (WMG) as the control was treated with gentamycin, dexamethasone and Chotrypsin. The course of the treatment was three months and hemorheology change was analysed before and after the treatment. After the above treatment, hystero salpingography was again performed on all patients except those with subsequent pregnancy. The results showed that the effective rate of catheter recanalization was 94%, and the pregnancy rate was 30% in CMG and 16% in WMG (P<0.05). The reocclusion rate of fallopian tube was 9.3% in CMG and 29.9% in WMG (P <0.05). The difference of hemorheology change was significant between the two groups (P< 0. 01) and between before and after treatment in CMG (P< 0. 01). This study showed that catheter recanalization was effective in mechanically recanalizing the occluded fallopian tube and that the Chinese medicine used in the treatment effective in lowing the concentration of blood and thus inhibiting the reocclusion and re-adhesion of the fallopian tube.     

Clinical and Experimental study on chronic Prostatitis with blood stasis Syndrome treated by prostatitis recipe

ｒｏｍ１９８６ｔｏ１９９３,ｔｈｅａｕｔｈｏｒｓｔｒｅａｔｅｄ５６１ｐａｔｉｅｎｔｓｏｆｃｈｒｏｎｉｃｐｒｏｓｔａｔｉｔｉｓ＂ｂｌｏｏｄｓｔａｓｉｓｔｙｐｅ＂ｗｉｔｈｐｒｏｓｔａｔｉｔｉｓｒｅｃｉｐｅ（ｄｅｃｏｃｔｉｏｎａｎｄｉｔｓｅｘｔｒａｃｔｃａｐｓｕｌｅ）（１－３）,ａｎｄａｃｑｕｉｒｅｄｇｏｏｄｅｆｆｅｃｔｓ．Ｓｉｍｕｌｔａｎｅｏｕｓｌｙ,ａｎｅｘｐｅｒｉｍｅｎｔａｌｓｔｕｄｙｏｎｔｈｅｍｅｃｈａｎｉｓｍｗａｓｃａｒｒｉｅｄｏｕｔ．ＣＬＩＮＩＣＡＬＳＴＵＤＹＣｌｉｎｉｃａｌＭａｔｅｒｉ…     

Clinical observation on patients with endometriosis treated by tonifying Kidney and removing blood stasis

Endometriosisisacommonlyandfrequentlyencounteredgynecologicaldisease.FollowingthetheoryoftraditionalChinesemedicine(TCM),'theKidneyisinchargeofreproduction",andi'theafterbirthandfetusareconnectedwiththeKidneyi',togetherwiththefollowingmanifestationsofendometriosis:dysmenorrhea,infertility,irregularmenstruation,dyspareunia,tinnitus,lassitudeintheloinandknees,hyposexuality,havingnodesandmasses,etc.,weholdthattheSyndromeDifferentiationforendometriosisshouldbe)'Kidneydeficiencyandbloodstasis"(l…     

Clinical study on relationship between Liver-Blood Stasis and Liver fibrosis

Inrecentyears,singlemodeoftreatmentprincipleapplyingactivatingbloodcirculationtoremovebloodstasistoretardliverfibrosishasalreadybeenchanged.ItisreportedthatmultipletreatmentprinciplessuchasdispersingthestagnatedLiverQi,replenishingQi,nourishingYin,warmingtheKidney,invigoratingtheSpleenandothersshowedgoodresultsinretardingliverfib.osis(l),whichmakeclinicaldoctorsfeelpuzzledinselectingrecipesandherbs,anditisdifficultinusingtraditionalChinesemedicinalherbstofurtherstudytheretardingliverfibrosis…     

Clinical and experimental study on treatment of chronic hepatitis B with Yanggan Aoping Mixture

Objective: To assess the efficacy of Yanggan Aoping Mixture ( , YGAPM) in treating hepatitis B.Methods: Patients suffered from chronic hepatitis B were treated with YGAPM. Observe their short- and long-term efficacy and the change of serum hepatitis B virus marker. In experiment, the effects of YGAPM in treating rat’s liver injury as well as HBV-infected tree shrew and duck HBV-infected ducks were observed.Results: In 79 cases of chronic persistent hepatitis, the markedly effective rate was 60.76%, and further raised to 70.00% in follow-up study in 40 cases. In 73 cases of chronic active hepatitis, the markedly effective rate was 60.27%, and further raised to 62.50% in 32 follow-up cases. In the treatment group, 85 of the 119 cases (71.43%) with HBeAg-positive were converted to negative. Whereas in the control group, only 40 of 89 HBeAg-positive cases (44. 94%) were converted to negative,P < 0. 01. Results of experimental study showed that negative conversion rate of tree shrew infected with HBV marker was raised, while the duck HBV DNA in infected duck blood was inhibited. Those compared with the control group separately, the difference was markedly significant.Conclusion: YGAPM is an effective drug in treating chronic hepatitis B, and it could effectively convert the marker to negative.     

Study on treatment of uloerative colitis with Liver-Qi stagnation by nourishing the Liver

Objective: To study the links between stagnation of the Liver-Qi and the pathogenesis of ulcerative colitis (UC) were studied clinically and experimentally.Methods: Using the principle of nourishing the Liver in the treated group and invigorating the Spleen in the control group.Results: The effective rate was 96% in the treated group and 82% in the control group, and the difference was significant (P<0.05); the formation rate of E rosettes and the transformation rate of lymphocytes were significantly raised in the two groups compared with the pre-treatment period, and the rate of adjusting dysfunction of vegetative nerve system in the treated group was evidently higher than that in the control group (P < 0.01). The level of intestinal styrenated phenol (SP) and vasoactive intestinal polypeptide (VIP) of UC model were determined in rats, and results showed that the level of SP and VIP significantly increased in the UC model group. They were markedly lowered in the treated group compared with model group (P < 0. 01) and there was significant difference from the control group (P<0.01).Conclusion: Wei Chang Ning , a drug to nourish the Liver, had the action of regulating neurological-endocrinological (gastrointestinal hormone)-immunological system.     

Clinical and experimental study on treatment of mid-late stage rheumatoid arthritis with Shuguan Granules ())

Objective: To explore the therapeutic mechanism of Shuguan Granule ( , SGG) in treating mid-late stage rheumatoid arthritis (RA).Methods: Based on the principle of reinforcing Kidney to treat arthritis, removing phlegm to relieve stasis, two SGG, Shuguan Wenjing (SW) and Shuguan Qingluo (SQ) were prepared and used to treat 44 and 43 mid-late stage patients of RA respectively. The clinical results were compared with those of Wangbi Granule. Animal experiments on the effect of these two SGG were conducted.Results: The total effective rate of the SW, SQ and control group was 88.64%, 93.02% and 73.17% respectively. Results of experimental studies in rats with adjuvant-induced arthritis showed that the two SGG obviously raise the level of serum superoxide dismutase, and lowered the levels of serum interleukin-1, plasma prostaglandin E₂ and thromboxane B₂, as compared with the model group, the difference was significant (P < 0.05,P < 0.01).Conclusion: The two SGG have a comprehensive function of anti-inflammatory, analgesic, antioxidatory, antihypercoagulatory and immunoregulatory effect.     

Effect of prevention and treatment of murine acute viral myocarditis and protection of lymphoid organ atrophy with xinkang oral liquid

Objective

The effect of prevention and treatment of Xinkang oral liquid, ( , XKOL) on experimental coxsackievirus B₃ (CVB₃) myocarditis mice model were investigated.

Methods

The mice were inoculated intraperitoneally with 0.3 ml of 10⁵ TCID₅₀ of CVB₃ to induce acute viral myocarditis model. These mice were divided into model control group (Group A), prevention high dosage group (Group B) and prevention low dosage group (Group C), treatment high dosage group (Group D) and treatment low dosage group (Group E), respectively. In addition, XKOL control group (Group F) and normal control group (Group G) were not infected with CVB₃ intraperitoneally. The administration of XKOL in Group B and C began 2 days before virus infection. All animals were sacrificed on day 20 for evaluation.

Results

Histological examination showed extensive myocardial necrosis and cell infiltration in most of Group A mice, but necrosis and cell infiltration were less severe in Group B,C,D and E mice. Thymus weight in Group B,C,D and E mice were heavier and less cell depletion occurred than those in Group A.

Conclussion

The XKOL could effectively inhibit myocardial CVB₃ replication, reduce the myocardial inflammatory response, lower incidence rate of myocarditis and prevent the disease associated lymphoid organ atrophy in this animal models.     

Bailong tablet and chemotherapy in the treatment of moderate and advanced cancersand chemotherapy in the treatment of moderate and advanced cancers

Objective: To determine the effectiveness of Bailong Tablet and chemotherapy on treatment of moderate and advanced cancers.Methods: The patients were divided randomly into two groups. The treated group (n= 41) was treated with chemotherapy plus Bailong Tablet; and the control group (n= 22) treated with chemotherapy alone. Treatment effectiveness was measured with Karnofsky scores.Results: Karnofsky performance status in the treated group before and after treatment was 66.0 ± 8.7 and 68.0 ± 5.9 respectively, that in the control group was 67. 0 ± 6.8 and 53. 0 ± 7.3 respectively. The completion rate of chemotherapy in the treated group (92.6%, 38/41) was higher than that in control (77.2%, 17/22,P < 0.05). The side-effects caused by chemotherapy in the treated group were lower than that in the control group (P < 0.05). CD₃ ⁺, CD₄ ⁺ and CD₄ ⁺ /CD₈ ⁺ ratio were higher in the treated group.Conclusion: Bailong Tablet can enhance the therapeutic effect and reduce the side-effects of chemotherapy. One possible mechanism is the modulation of cellular immune function.     

Clinical Study of Weishu capsule () in treating precancerous lesions of chronic atrophic gastritis) in treating precancerous lesions of chronic atrophic gastritis

Objective: To explore the therapeutic effect and mechanism of Weishu capsule (WSC), in treating precancerous lesions of chronic atrophic gastritis (CAG).Methods: Seventy-five cases of precancerous lesions of CAG were distributed to two groups randomly, the WSC group (45 patients) treated with WSC taken orally and the Weining granule (WNG) group (30 patients) treated with Weining granule orally. The treatment course of both groups were 6 months. The clinical and pathological effects and the changes in scores of atrophy, intestinal metaplasia (IM) and its subgroups, dysplasia, carcinoembryonic antigen and proliferating cell nuclear antigen expression were observed before and after treatment.Results: The total effective rate was 91% and the effective rate of atrophy, IM, dysplasia in the WSC group were 64%, 67% and 65% respectively, and the respective scores were lowered significantly after treatment. Compared with the WNG group, the difference between the two groups was statistically significant (P < 0.01,P < 0.05). Immunohistochemical test showed that WSC could inhibit the subgroups of IM, circinoembryonic antigen and proliferating cell nuclear antigen expression significantly (P < 0.01).Conclusions: WSC could significantly improve the clinical and pathological changes in the precancerous lesions of CAG. The mechanism might be related to the inducing and promoting effect of WSC on the differentiation and maturity of IM cells and dysplasia cells. WSC could also inhibit and correct the abnormal proliferation of cells.     

Effect of Reduqing on plasma lnterleukin-8 and nitric oxide in rabbits with endotoxin induced disseminated intravascular coagulation

Objective: To investigate the effects of Reduqing (RDQ, ) on plasma interleukin-8 (IL-8), NO₂ ^- /NO₃ ^-, complement 5a (C_5a) and polymorphonuclear neutrophilic leukocyte (PMN) chemotaxis Index (CI) in rabbits with endotoxin-induced disseminated intravascular coagulation (DIC).Methods: Endotoxin-in-duced DIC model made by injection of Lipopolysacchrides (LPS) was used in the experiment. Above-mentioned indexes were determined before and after RDQ treatment and compared with blank and dexamethasone treated group.Results: Plasma IL-8, NO₂ ^- /NO₃ ^-, C_5a and CI level of PMN increased markedly in the model group, which were confirmed pathologically with obvious damage of tissues or organs. In the RDQ group, the abovem entiond parameters and damage of tissues or organs were reduced significantly (P < 0.01).Conclusion: IL-8 and NO might be involved in pathogenesis of endotoxin-induced DIC, and RDQ could be used in preventing or treating DIC through the mechanism of regulation of cytokines network.     

Effects of supplemented Taohe Chengqi Decoction in treating insulin resistance in rats with non-insulin Dependent Diabetes Mellitus) in treating insulin resistance in rats with non-insulin Dependent Diabetes Mellitus

Objective: To investigate the effect of supplemented Taohe Chengqi Decoction ( , STHCQD) in treating non-insulin dependent diabetes mellitus (NIDDM).Methods: The model of rats with NIDDM was formed with injection of streptozotocin and fed on high calorie diet to study the effects of STHCQD on the release of insulin sensitivity.Results: (1) Fasting serum glucose, serum insulin, intake of food and water were significantly decreased (P < 0.05- 0.01) in STHCQD-treated diabetic rats as compared with untreated diabetic rats, while the insulin sensitivity was significantly increased (P < 0.05). (2) The liver cell membranes from STHCQD-treated diabetic rats released the quantity of insulin receptor which inhibited adenylate cyclase activity, but this effect was blunted in untreated diabetic rats (P < 0.05). (3) A significantly increased glucose oxidation in adipocyte of STHCQD-treated diabetic rats was found as compared with those of untreated diabetic rats (P < 0.05).Conclusions: STHCQD therapy increased sensitivity and responsiveness of target cells to insulin, i.e. it might decrease insulin resistance at receptor sites and post-receptor sites in rats with NIDDM, but could not reverse the insulin resistance.     

Effect of Sanhuang Jiangtang recipe on insulin peripheral resistance in Type II diabetes mellitus) recipe on insulin peripheral resistance in Type II diabetes mellitus

Objective: To observe the effect of Sanhuang Jiangtang recipe ( , SHJT) on insulin peripheral resistance in Type II diabetes mellitus (DM).Methods: Ninety-five patients with type II DM were randomly divided into two groups. Fifty-three cases of SHJT group were given decoction and tablets of SHJT orally for 4–6 months. The efficacy was compared with that of 42 cases treated with Glipizide as the control. Before and after treatment standard steamed bread meal test was performed to measure the insulin peripheral sensitivity, insulin release to glucose and insulin sensitivity index.Results: (1) The total effective rates of improving insulin peripheral resistance and reducing blood sugar in SHJT group were 79. 2% and 80.1 %, which was equivalent to levels in the control group, but SHJT recipe was more effective in relieving symptoms of Qi deficiency and signs of blood stasis. (2) In SHJT group, the insulin peripheral sensitivity and insulin sensitivity index were significantly increased (P < 0.05 and 0.01), meanwhile the fasting blood sugar and blood sugar area were reduced (P < 0.05), but the change of insulin release to glucose was blunted. (3)The lowering of blood sugar in SHJT group was significantly negative correlated with the changing of degree of insulin peripheral sensitivity and index of insulin sensitivity (P < 0.01 and 0.05), but not with that of insulin area.Conclusion: It suggested that the treatment of SHJT recipe might decrease insulin peripheral resistance (partial reversal) by means of reducing hyperinsulinemia and improving insulin sensitivity.