MTA修复犬牙髓室底穿孔的组织学评价

目的 用MTA、Dycal、GIC即时修复不同大小髓室底穿孔，从组织学角度评价三种材料对牙周组织的炎症反应和硬组织形成的影响。方法 选择3条成年杂种犬的42颗后牙，用随机法先将一侧牙分为小穿孔组，另一侧牙分为大穿孔组；每侧再分为三个亚组，分别用MTA、Dycal、GIC修复。4个月后处死动物，标本经处理，光学显微镜下观察材料下方牙周组织的炎症细胞及其程度、上皮增生以及硬组织形成情况。结果 MTA组的14例中5例无炎症反应，其余9例仅有轻度至中度的炎症反应，4例有牙骨质形成，周围可见成牙骨质细胞；Dycal组材料下方均引起牙周组织中度或重度的炎症反应，2例小穿孔处有不规则的钙化物沉积，同时伴有大量炎症细胞，上皮增生多见(6／14)；GIC组除1例大穿孔标本有少量散在的炎症细胞及纤维组织包绕，其余标本均可见中度或重度的炎症反应，有的伴有上皮增生(4／14)。上皮增生与穿孔大小有一定关系。结论 MTA用于即时修复犬牙髓室底穿孔，能诱导硬组织形成，对组织的刺激小，效果优于GIC和Dycal。     

两种材料修复比格犬髓室底穿孔的效果评价

目的:评价MTA和光固化氢氧化钙修复比格犬牙齿髓室底穿孔的效果.方法:3只成年比格犬的36颗前磨牙根管治疗后做髓室底穿孔模型,随机分为两组,分别采用MTA和光固化氢氧化钙修复穿孔.另外24颗磨牙根管治疗后随机分为阳性和阴性对照组,阳性对照组做髓室底穿孔模型后以聚氯乙烯膜覆盖穿孔,阴性对照组不穿孔.分别于术后1,4,12...     

不同材料修复髓室底穿孔渗漏的实验研究

目的:寻求修复髓室底穿孔较理想的修复材料。方法:在离体牙上采用染料渗入法检测LGIC、CPC、HA、LCR和LCH修复髓室底穿孔后的渗漏情况。结果:5组穿孔修复材料的渗漏,从小到大排列顺序为:LGIC组、CPC组、LCH组、LCR组和HA组,5组间渗漏差异有极显著性(P<0.01)。LGIC组和CPC组之间渗漏差异无显著性(P>0.05),两组渗漏均明显小于LCH组、LCR组和HA组(P<0.01)。结论:LGIC、CPC修复髓室底穿孔具有良好的边缘密封性。     

BME-10X型胶原/羟磷灰石人工骨引导 比格犬牙周组织再生的实验研究

目的 评估BME-10X型胶原/羟磷灰石人工骨引导比格犬牙周组织再生的能力.方法 选取18月龄雄性比格犬4只,进行牙周基础治疗.基础治疗结束1周后,选取下颌第3、4前磨牙,同颌对侧同名牙随机纳入实验组或者对照组,制造牙周缺损,实验组植入BME-10X型胶原/羟磷灰石人工骨,对照组不植入任何材料.术后12周处死动物,进行...     

复方五倍子作为髓室底穿孔屏障材料的微渗漏研究

目的:探讨复方五倍子用作髓室底穿孔屏障材料对封闭性的影响.方法:在55个离体牙上制造髓室底穿孔模型后,分别用银汞合金(A组)、复合树脂(R组)、复方五倍子作屏障银汞合金修复(AM组)、复方五倍子作屏障复合树脂修复(RM组)及MTA(MTA组)修复,通过染料渗漏法测定各组渗漏情况.结果:阳性对照组(PC)为完全渗漏,阴性对照组(NC)无染料渗入.所有实验组均有不同程度的渗漏.微渗漏值由大到小依次为:A组、R组、AM组、MTA组、RM组,各组间两两比较,除AM组和R组、AM组和MTA组外,均有统计学差异(P＜0.05).AM组和RM组无超填.结论:复方五倍子作为屏障材料来修补髓室底穿孔可以减轻微渗漏,并能有效防止修复材料的超填.     

磷酸钙骨水门汀修复髓室底穿孔后渗漏的实验研究

目的 :寻求修复髓室底穿孔较好的修复材料。方法 :在离体牙上采用染料渗入法检测CPC、LGIC及CPC与LGLC夹层技术修复髓室底穿孔后的渗漏情况。结果 :三组穿孔修复材料的渗漏从小到大排列顺序为 :LGLC组、夹层技术组、CPC组 ,三组间渗漏无显著性差异 (P >0 .0 5 )。结论 :CPC具有良好的密封性能 ,其密封性与LGIC相近似。结合CPC的其它性能 ,认为CPC可作为髓室底穿孔较理想的修复材料之一。     

贝壳多孔羟基磷灰石基骨修复材料和骨形成蛋白-2联合应用引导犬牙周组织再生效果初步评估

目的初步评估贝壳多孔羟基磷灰石基骨修复材料及该材料和骨形成蛋白-2联合应用引导比格犬牙周组织再生的效果。方法选取18月龄比格犬6只,牙周基础治疗后1周,在下颌第二、三、四前磨牙,建立急性牙周骨缺损模型,依照分组情况进行不同治疗。实验组（T组）植入骨修复材料和骨形成蛋白-2;阴性对照组（NC组）植入骨修复材料;空白对照组（BC组）不植入任何材料。实验设计采取同颌同名牙对照,同一只比格犬的3对同颌同名牙分别为：空白对照组和阴性对照组,阴性对照组和实验组,空白对照组和实验组。术后12周,处死动物,Micro-CT检查并对数据进行统计学分析。结果材料植入后,未见材料溢出,植入局部和全身都未见明显不良反应。3组缺损都有一定程度骨再生,以T组再生组织量最多,BC组最少。Micro-CT结果显示：T组、NC组和BC组的骨再生平均高度为（4.50±0.47）mm（、1.75±0.42）mm和（0.87±0.31）mm。NC组和BC组相比,差异有统计学意义（P〈0.05）。T组与NC组和BC组相比,差异均有统计学意义（P〈0.05）,且有临床意义。结论贝壳多孔羟基磷灰石基骨修复材料和骨形成蛋白-2联合应用于比格犬,可以获得更好的引导组织再生效果。     

磷酸三钙修复髓室底穿通的实验和临床应用研究

用磷酸三钙作为髓室底穿通后的屏障和修复材料进行了动物实验和临床应用研究。实验中，对照组为氢氧化钙。动物实验发现磷酸三钙封闭穿孔后，１个月这内炎症反应较明显；实验后１－２个月的样本较明显转为正常。实验后３月，穿底下方可见有新骨形成。而氢氧化钙组中，早期炎症更明显，后期骨质增生不明显。临床上选用磷酸三钙作为髓室底穿通的屏障和修复材料共治疗１０例慢性尖炎伴意外穿底的患牙，结果显示，磷酸三钙垫底充填３－１     

骨形成蛋白复合牙本质陶瓷修复髓室底穿孔的实验研究

目的：用骨形成蛋白复合牙本质陶瓷修复髓室底穿孔，以氢氧化钙作为对照组。方法：术后６个月观察骨形成蛋白复合牙本质陶瓷修复髓室底穿孔的生物学作用。结果：骨形成蛋白复合牙本质陶瓷具有诱导骨样组织形成。结论：骨形成蛋白复合牙本质陶瓷可以用于修复髓室底穿孔。     

骨形成蛋白复合牙本质陶恣修复髓室底穿孔的实验研究

用骨形成蛋白复合牙本质陶瓷修复髓室底穿孔，以氢氧化钙作为对照组。方法：术后６个月观察骨形成蛋白复合牙本质陶瓷修复髓室底穿孔的生物学作用。结果：骨形成蛋白 合牙本质陶瓷具有诱导骨样组织形成。结 论：骨形成蛋白复合牙本质陶瓷可以用于修复髓室底穿孔。     

支抗种植体的组织学研究进展

支抗种植体是近年来国内外正畸学研究的热点之一。作者通过组织学研究的文献回顾,反映出支抗种植体微螺钉化、自攻化的发展方向。虽然种植支抗的加力时机尚有争论,但微螺钉存在骨结合,可即刻加力、长期应用,牙周损伤可自愈等新观点对传统认识提出了挑战。     

Healing of implant dehiscence defects with without expanded polytetrafluoroethylene membranes: a controlled clinical and histological study and histological study

Bone augmentation around implant fixtures using the technique of guided tissue regeneration has been described in a number of reports. The aim of this study was to evaluate bone fill around dehiscence defects at matched defects randomly allocated to test (expanded polytetrafluoroethylene; Gore‐Tex) or control treatments within the same patient. Six edentulous subjects were treated with overdentures supported by 2 fixtures ad modum Brinemark. In addition, 2 experimental 7 mm fixtures with nearly identical dehiscence defects on the labial aspect were placed in the anterior part of the mandible. Baseline defect heights ranged from 2.5 to 4.0 mm with 2 to 4 threads exposed. The test fixture dehiscence was covered with a Gore‐Tex membrane secured with a cover screw. The experimental fixtures were exposed after 5 months at stage 2 surgery and measurements and photographs repeated. The fixtures were then removed with a trephine and processed for histological evaluation. Regeneration of a hard tissue resembling bone was observed in 4 of 6 cases treated with Gore‐Tex, amounting to between 95 and 100% elimination of the dehiscence and total coverage of the threads. However, in one of these cases, histological evaluation showed that it was soft tissue and not bone, and in a further case soft tissue regeneration was apparent both clinically and histologically. In another case there was no significant regeneration at either test or control defects. Although there was a trend for the Gore‐Tex treated dehiscences to respond more favourably, the differences in clinical and histological measurements between test and control failed to reach statistical significance.     

成人髁突增殖带的计量组织学研究

为进一步了解成人髁突增殖带细胞数量、厚度与年龄的关系，采用连续切片、多点测量法对４３例（１９～７４岁）髁突增殖带细胞排列及其厚度进行了观察。按不同年龄分为青年组（≤４５岁）、中年组（４６～５９岁）和较老年组（６０～７４岁）。对同年龄组不同区及同区不同年龄组的增殖带细胞数量、厚度与年龄的关系进行了统计分析。结果显示：不同年龄组中，增殖带的平均厚度不一，且随年龄增加细胞逐渐减少，厚度变薄，各年龄组间差异有高度显著性（Ｐ＜０．０１），中年组与较老年组间增殖带厚度差异无显著性（Ｐ＞０．０５）。各区厚度与年龄增长呈负相关关系。     

Oral vegetable granuloma: ultrastructural and histological study

An unusual giant-cell granulomatous inflammatory oral lesion is characterised by the presence of hyaline rings and by a lack of agreement on their nature, opinions ranging from their being hyaline degenerative blood-vessels to remains of leguminous cells. Ten such lesions and a variety of vegetables have been examined in the present investigation. Light-microscopical examination of the lesions revealed the hyaline rings surrounded by inflamed granulation tissue with associated multinuclear giant cells. Electron-microscopical examination showed them to consist of a material similar to that of vegetable cell walls, sometimes with an associated superficial layer of collagen. The observations indicate that the lesions consist of vegetable remains with an associated foreign-body type of inflammatory response.     

A histological study of experimental gingivitis in man

Experimental gingivitis was induced in 21 individuals. Oral hygiene was abolished on the buccal and interproximal tooth surfaces of one side of the mouth in the mandibular premolar and molar area. The other tooth surfaces served as controls and were subject to thorough oral hygiene measures. After 15–17 days, when clinically manifest gingivitis had developed in the non-cleansing side of the majority of the subjects, biopsies were obtained from that side as well as from the control side. Histological examination of tissue from the experimental side revealed inflammatory infiltrates largely confined to the connective tissue adjacent to the pocket epithelium. The cells were mainly medium-large and small lymphocytes, macrophages and fibroblasts. A few neutrophilic leukocytes were observed mostly inside the vessels and adjacent to and within the pocket epithelium. Immature and mature plasma cells were seen in the central regions of the connective tissue, but were scant or absent nearer to the pocket epithelium The distribution of the different types of mononuclear cells might be consistent with a transformation of lymphocytes into plasma cells.     

Oral vegetable granuloma: ultrastructural and histological study

An unusual giant-cell granulomatous inflammatory oral lesion is characterised by the presence of hyaline rings and by a lack of agreement on their nature, opinions ranging from their being hyaline degenerative blood-vessels to remains of leguminous cells. Ten such lesions and a variety of vegetables have been examined in the present investigation. Light-microscopical examination of the lesions revealed the hyaline rings surrounded by inflamed granulation tissue with associated multinuclear giant cells. Electron-microscopical examination showed them to consist of a material similar to that of vegetable cell walls, sometimes with an associated superficial layer of collagen. The observations indicate that the lesions consist of vegetable remains with an associated foreign-body type of inflammatory response.     

Radiological and histological study of osteomyelitis of the jaws

Fifty-six patients with maxillary and mandibular osteomyelitis were investigated radiologically and histopathologically. The radiographic changes were classified into 5 patterns: osteolytic, mixed, sclerosing, sequestrum and irregular trabeculation patterns. Osteomyelitis was classified into 3 histological types, based on the amount of bony trabeculae present. Type I bone consisted of severely sclerotic bone, type II bone had coarse trabeculae with fibrotic marrow spaces. In contrast, type III bone had thin trabeculae with occasional osteoblastemata. The correlations between the radiographic and histological patterns and differences between acute and chronic cases were examined. Acute cases were often of the osteolytic or sequestrum patterns, and had type I or II bone. Type II bone was the most common, and was found in all radiographic types. Type III bone was frequently seen in cases with an osteolytic or mixed radiographic pattern. It seemed that severely sclerotic bone with narrowed or occluded Haversian canals (type I bone) tended to develop following acute infection. A possible explanation of the relationship of 5 radiographic types is also discussed.