胃痞消对胃癌前病变大鼠血浆肿瘤坏死因子-α和白细胞介素-4表达的影响

【目的】观察胃痞消对脾虚证胃癌前病变(PLGC)大鼠血浆肿瘤坏死因子-α(TNF-α)和白细胞介素-4(IL-4)表达的影响。【方法】将SD大鼠分为6组:正常对照组,模型组,维酶素组(剂量为0.2 g·kg-1·d-1),胃痞消高、中、低剂量组(剂量分别为15、7.5、3.75 g·kg-1·d-1)。采用N-甲基-N’-硝基-N-亚硝基胍(MNNG)溶液(200μg/m L)自由饮用、小承气汤(2 m L/只,生药1 g/m L)灌胃法及饥饱失常等多因素造模18周复制脾虚证胃癌前病变模型,观察各组大鼠的组织病理学及行为学变化,检测血浆TNF-α及IL-4含量。【结果】模型组血浆TNF-α、IL-4含量较正常组显著降低(P<0.05);维酶素组、胃痞消高剂量组、胃痞消低剂量组血浆TNF-α含量较模型组显著升高(P<0.05),模型组与胃痞消中剂量组比较差异无统计学意义(P>0.05);维酶素组、胃痞消高剂量组、胃痞消中剂量组、胃痞消低剂量组血浆IL-4含量与模型组比较均显著升高(P<0.05)。【结论】胃痞消改善脾虚证胃癌前病变大鼠组织学变化的机制可能通过调节血浆TNF-α、IL-4含量变化及胃黏膜间质淋巴细胞及单核细胞的动员而实现。     

宁痫颗粒联合卡马西平对难治性癫痫大鼠血清IL-1β、IL-6、TNF-α影响的实验研究

目的：观察宁痫颗粒联合卡马西平对海人酸难治性癫痫大鼠血清IL-1β、IL-6、TNF-α水平的影响,探讨其作用机制.方法：Wistar雄性大鼠42只,按体质量随机分为假手术组、模型组、卡马西平＋宁痫颗粒高（0.03＋4.80/g·kg-1·d-1）、中（0.03＋2.40/g·kg-1·d-1）、低剂量组（0.03＋ 1.20/g·kg-1·d-1）、宁痫颗粒组（2.40/g· kg-1·d-1）和卡马西平组（0.03/g· kg-1·d-1）,每组6只.采用海马CA3局部注射海人酸制作大鼠癫痫模型,术后第二天予苯妥英钠药物筛选14d后成功制作难治性癫痫模型.各组分别灌胃2周后股动脉采血,收集各实验组大鼠血清,采用免疫酶联法（Elisa法）,测定各实验组大鼠血清IL-1β、IL-6、TNF-α水平.结果：与卡马西平组比较,宁痫颗粒低剂量联合卡马西平组大鼠血清IL-1β、IL-6含量显著降低（P＜0.05）,宁痫颗粒中、高剂量组联合卡马西平大鼠血清IL-1β、IL-6含量极显著降低（P＜0.01）;宁痫颗粒低、中、高剂量联合卡马西平组大鼠血清TNF-α含量极显著降低（P＜0.01）.结论：IL-1β、IL-6、TNF-α共同参与了免疫反应,其水平的变化间接反映了脑组织损伤程度.表明宁痫颗粒联合卡马西平具有一定的免疫抑制作用,比单用卡马西平疗效显著,说明两者联合具有协同作用,其机制可能是通过抑制炎症反应从而阻断癫痫发作,达到治疗癫痫目的.     

祛湿除痹中药对急性痛风性关节炎模型大鼠的抗炎机制研究

【目的】通过观察祛湿除痹中药对急性痛风性关节炎模型大鼠踝关节内急性炎症反应通道的影响,探讨其对急性痛风性关节炎模型的抗炎作用机制。【方法】复制急性痛风性关节炎模型,将40只Wistar大鼠随机分为5组(每组8只):空白对照组、模型对照组、秋水仙碱组(剂量为9 mg.kg-1.d-1)、中药高剂量组(剂量为54 g.kg-1.d-1)、中药低剂量组(剂量为27 g.kg-1.d-1)。连续灌药7 d后取材,观察各组对大鼠关节肿胀度及关节内炎症细胞、炎症因子白细胞介素及其受体(IL-1β、IL-1R)及髓样细胞分化因子(MyD88)表达的影响。【结果】各给药组炎症细胞计数、IL-1β、IL-1R、MyD88表达水平显著低于模型对照组(均P<0.05或P<0.01),其中中药高剂量组各炎症指标表达水平显著低于秋水仙碱组(均P<0.05),中药低剂量组各炎症指标与秋水仙碱组比较差异无统计学意义(P>0.05)。【结论】祛湿除痹中药可通过阻断IL-1R-MyD88信号通路而减轻急性痛风性关节炎模型大鼠踝关节内炎症反应。     

桃核承气汤对重症急性胰腺炎大鼠肠道功能的影响

【目的】探讨桃核承气汤对重症急性胰腺炎(SAP)大鼠肠道功能的影响及作用机制。【方法】将50只SD大鼠随机分为空白对照组、SAP模型组、桃核承气汤组(灌胃剂量为23.5 m L·kg-1·d-1)、奥曲肽组(腹腔注射剂量为10μg·kg-1·d-1)、联合用药组(桃核承气汤灌胃剂量为23.5 m L·kg-1·d-1,奥曲肽腹腔注射剂量为10μg·kg-1·d-1)。除空白对照组外,其余各组采用L-精氨酸腹腔注射复制大鼠SAP模型,造模成功后24 h取材,采用菌群记数法检测各组大鼠粪便菌群变化,生化测定各组大鼠血清淀粉酶(AMY)水平,采用酶联免疫吸附(ELISA)法检测各组大鼠血清白细胞介素(IL)-6、IL-10、肿瘤坏死因子(TNF)-α水平,采用苏木素—伊红(HE)染色法观察胰腺和大肠组织病理变化。【结果】与空白对照组比较,SAP模型组大鼠血清AMY、TNF-α、IL-6水平显著降低,IL-10水平显著升高(P0.05),肠球菌、肠杆菌增多,乳酸杆菌、双歧杆菌则大幅度骤减,胰腺、大肠组织结构破坏,大量炎症细胞浸润。与SAP模型组比较,3个治疗组大鼠血清AMY、TNF-α、IL-6水平显著升高(P0.05),IL-10水平显著降低(P0.05),肠球菌、肠杆菌减少,乳酸杆菌、双歧杆菌增加,胰腺、大肠组织病理变化均减轻,以联合用药组最明显。【结论】桃核承气汤对SAP大鼠的肠道功能有良好的改善作用,可减轻炎症反应,改善肠道菌群,抑制SAP病情的恶化。     

滋肾宁神胶囊对 D-半乳糖所致亚急性衰老模型大鼠的影响

[目的]观察滋肾宁神胶囊对D-半乳糖所致亚急性衰老模型大鼠的影响.[方法]选用SD大鼠随机分成空白对照组,模型组,七宝美髯丸组(剂量为2 g·kg-1·d-1),滋肾宁神丸组(剂量为3.33 g·kg-1·d-1),滋肾宁神胶囊高、中、低剂量组(剂量分别为9.96、4.98、2.49 g·kg-1·d-1);除空白对照...     

白子菜提取物对急性痛风性关节炎大鼠模型的抗炎免疫作用

【目的】探讨白子菜提取物对急性痛风性关节炎的抗炎免疫作用及机制。【方法】复制急性痛风性关节炎大鼠模型,将72只Wistar大鼠随机分为9组(每组8只):空白组,模型组,秋水仙碱组(剂量为0.2 mg·kg-1·d-1),白子菜水提取物低、中、高剂量组(剂量分别为2.5、5、10 g·kg-1·d-1),白子菜醇提取物低、中、高剂量组(剂量分别为5、10、20 g·kg-1·d-1)。连续灌药7 d后取材,观察各组大鼠关节活动情况(炎症指数、功能障碍指数、足肿胀度),采用酶联免疫吸附(ELISA)法检测血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平,定量聚合酶链反应(q PCR)法检测单核细胞半胱天冬酶-1(caspase-1)、凋亡相关斑点样蛋白(ASC)、NLRP3炎性体(NLRP3)表达,并分析其关系。【结果】各药物处理组对急性痛风性关节炎大鼠活动障碍均有缓解作用;秋水仙碱组TNF-α表达水平显著低于模型组(P<0.05);秋水仙碱组及白子菜醇提取物中剂量组IL-1β、caspase-1、ASC、NLRP3表达水平显著低于模型组(P<0.05);IL-1β与caspase-1、ASC、NLRP3存在显著相关性,TNF-α与ASC存在显著相关性(P<0.05)。【结论】秋水仙碱及中剂量白子菜醇提物能通过抑制NLRP3炎性体的表达,从而抑制IL-1β相关的炎性过程,发挥其抗炎免疫作用。     

尿毒清胶囊及其拆方对5/6肾切除大鼠肾组织TGF-β1 mRNA表达的影响

[目的]观察尿毒清胶囊及其拆方对慢性肾功能哀竭(CRF)模型大鼠肾组织转化生长因子β1(TGF-β1)mRNA表达的影响.[方法]采用5/6肾切除法复制CRF大鼠模型,造模成功的SD大鼠随机分为模型组、黄芪组(剂量1.5 g·kg-1·d-1)、丹参组(剂量为1 g·kg-1·d-1)、大黄组(剂量为1 g·kg-1·d-1)、黄芪丹参大黄组(剂量为1 g·kg-1·d-1)、尿毒清组(剂量为1 g·kg-1·d-1);各给药组灌胃给药6周后,检测各组大鼠血清尿素氮(BUN)、肌酐(SCr)水平,并采用逆转录聚合酶链反应检测各组大鼠肾组织TGF-β1 mRNA的表达水平.[结果]各给药组均可降低血清BUN、SCr水平,与模型组比较均有显著性差异(P＜0.05或P＜0.01),其中尿毒清组降低SCr水平作用优于大黄组(P＜0.05);TGF-β1 mRNA在各组肾组织中均有不同程度的表达,其中模型组表达最强烈,尿毒清组和黄芪丹参大黄组表达最轻.[结论]尿毒清治疗CRF的作用与其能下调TGF-β1 mRNA在肾组织中表达有关,且中药复方作用优于单味中药.     

当归补血汤对糖尿病合并抑郁症模型大鼠血清炎症因子的干预作用

目的:探讨当归补血汤治疗糖尿病并发抑郁症的可能作用机制。方法:40只大鼠其中8只Wistar大鼠为空白对照组,其余32只GK大鼠随机分为模型组、氟西汀组及中药高、低剂量组,每组8只。除空白对照组外,其余各组通过慢性温和不可预知性刺激(chronic mild unpredictable stimulation,CUMS)建立糖尿病并发抑郁症模型。应激造模的同时中药高、低剂量组分别按照4 g·kg~(-1)·d~(-1)和8 g·kg~(-1)·d~(-1)剂量给予当归补血汤,氟西汀组按照2.2 mg·kg~(-1)·d~(-1)剂量给予氟西汀,空白对照组和模型组给予等体积的生理盐水溶液,同时检测大鼠体质量及随机血糖。灌胃28 d后,Elisa法检测大鼠血清中炎症因子白细胞介素-6(interleukin-6,IL-6)、白细胞介素-8(interleukin-8,IL-8)、白细胞介素-10(interleukin-10,IL-10)、超敏C反应蛋白(C-reactionprotein protein,CRP)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的含量。结果:模型组大鼠血糖一直高于空白对照组,而与模型组比较,给药后大鼠血糖明显下降,差异有统计学意义(P0.05)。与空白对照组比较,模型组大鼠血清中IL-10表达显著降低,TNF-α、CRP、IL-6和IL-8的表达均显著升高,差异有统计学意义(P0.001);与模型组比较,氟西汀组、中药低剂量组和中药高剂量组大鼠血清中IL-10的表达均显著提高,但TNF-α、CRP、IL-6和IL-8的表达均显著降低,差异有统计学意义(P0.05或P0.01)。结论:当归补血汤可降低糖尿病并发抑郁症大鼠随机血糖水平,抑制大鼠全身炎症反应,是该方治疗糖尿病并发抑郁症的可能机制之一。     

安心颗粒对心衰大鼠血清TNF-α、IL-6及心肌细胞凋亡的影响

[目的]观察安心颗粒对慢性心力衰竭(CHF)模型大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平及心肌细胞凋亡的影响.[方法]将50只雌性SD大鼠随机分为正常对照组、模型组、卡托普利组、安心颗粒低荆量组和安心颗粒高剂量组.采用阿霉素腹腔注射造模,每周1次,连续6周.造模同时,卡托普利组及安心颗粒低、高剂量组分别按6.25 mg·kg-1·d-1、0.8 g·kg-1·d-1、1.6 g·kg-1·d-1剂量灌胃给药.造模结束后,分别测定各组血清TNF-α、IL-6水平与心肌细胞凋亡指数(I凋亡).[结果]模型组血清TNF-α、IL-6水平及,I凋亡均显著性升高(P＜0.05);安心颗粒低、高剂量组均可显著性降低模型大鼠血清TNF-α、IL-6水平及I调亡(P＜0.05或P＜0.01),且呈现一定剂量效应关系.[结论]安心颗粒可通过调控炎性细胞因子,抑制心肌细胞凋亡,阻止心力衰竭的发展.     

养阴活胃合剂对慢性萎缩性胃炎大鼠血清胃蛋白酶原和胃组织水通道蛋白的影响

目的 探究养阴活胃合剂治疗慢性萎缩性胃炎（chronic atrophic gastritis,CAG）的作用机制。方法 将66只SD大鼠随机分为正常组,模型组,胃复春组,养阴活胃合剂高、中、低剂量组。采用综合法（氨水+脱氧胆酸钠+不同浓度乙醇＋饥饱失常法）复制CAG模型,连续12周。采用ELISA法检测大鼠血清胃蛋白酶原Ⅰ（pepsinogen Ⅰ,PGⅠ）、胃蛋白酶原Ⅱ（pepsinogen Ⅱ,PGⅡ）水平及胃蛋白酶原比值（pepsinogen ratio,PGR）。采用RT-qPCR法检测胃组织水通道蛋白（aquaporin,AQP）1、AQP3和AQP4 mRNA表达水平。〖JP〗结果 与模型组比较,养阴活胃合剂高、中、低剂量及胃复春可以提高大鼠胃液总酸度,改善胃液pH值（P＜0.05）;各给药组大鼠血清PGⅠ水平和PGR升高,PGⅡ水平降低;各给药组大鼠AQP1 mRNA表达水平降低,养阴活胃合剂中、低剂量组与胃复春组大鼠AQP3 mRNA表达水平明显升高（P＜0.05）,养阴活胃合剂高、中剂量组和胃复春组AQP4 mRNA表达水平显著升高（P＜0.05）。结论 养阴活胃合剂通过升高血清PGⅠ水平和PGR,上调AQP3、AQP4 mRNA表达水平,降低PGⅡ水平和下调AQP1 mRNA表达水平改善CAG大鼠的胃酸分泌。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.