MONOCLONAL ANTI-TYPE 2 H: AN ANTIBODY DETECTING A PRECURSOR OF THE A AND B BLOOD GROUP ANTIGENS

A monoclonal antibody was produced which binds specifically to Type 2 H antigen of the ABO blood group system. The antibody, H11, is an IgM molecule which reacts by direct agglutination of red cells with the same pattern as other anti-H reagents such as Ulex europaeus lectin. The specificity was determined by inhibition and adsorption with chemically defined oligosaccharides. H11 also reacted with poly(glycosyl)ceramide purified from group O red cells and glycoprotein H purified from human stomach mucosa and meconium. H11 differs from Ulex lectin, however, in that it was not inhibited by saliva from ABH secretor individuals or by glycoprotein H purified from human ovarian cysts or submaxillary glands.     

OOCYTES REACT WITH ANTIBODY DIRECTED AGAINST H-2 BUT NOT Ia ANTIGENS

Mouse oocytes at the dictyate stage were tested for the presence of H-2 antigens and Ia antigens, by means of indirect immunofluorescence. Oocytes reacted positively with NIH alloantisera, but were negative with anti-Ia region alloantisera. Results with monoclonal antibodies indicate very low antigen density on oocytes.     

抗t—PA单克隆抗体的制备及初步应用

应用锗心ｔ－ＰＡ和人黑色素瘤细胞分泌的ｔ－ＰＡ抗原，经腹腔及脾内免疫ＢＡＬＢ／Ｃ小鼠。用细胞融合技术制务杂瘤细胞，细胞融合率达８５％获得四株抗ｔ－ＰＡ杂交瘤细胞株，鼠腹水抗体效价达１：１０＾５；Ｗｅｓｔｅｒｎ－Ｂｌｏｔ结果表明该单抗所结合的抗原分子量与ｔ－ＰＡ相符。     

Tamm-Horsfall糖蛋白单克隆抗体制备及其临床应用

用0.58mol/L NaCl沉淀提取Tamm-Horsfall糖蛋白(THP)制备了三株抗THP单克隆抗体(McAb)。免疫印迹技术(Immunoblotting Technigue,IBT)证明这些McAb特异性地和94kD THP抗原结合,ELISA抑制试验表明三株McAb识别THP抗原的不同位点。用McAb双夹心ELISA法测定尿THP的含量,表明尿THP含量与血尿素氮(BUN)、血肌酐(Scr)呈显著负相关(P<0.01),与内生肌酐清除率(Ccr)呈明显正相关(P<0.01),BUN、Scr、Ccr正常的各种肾病尿THP含量无明显异常(P<0.05)。测定尿THP含量可作为判断肾功能损害程度的良好指标。     

白细胞介素6单克隆抗体的研制

采用纯化的工程菌表达产物白细胞介素６免疫ＢＡＬＢ／Ｃ小鼠,取小鼠脾细胞与骨髓瘤细胞（ＳＰ２／０）在ＰＥＧ作用下进行融合,通过ＥＬＩＳＡ筛选并多次亚克隆,获得了１株能分泌抗ＩＬ－６单克隆抗体的杂交瘤细胞Ｉｃ－１．３．４．５。Ｗｅｓｔｅｒｎ ｂｌｏｔ显示：抗体结合抗原的分子量与ＩＬ－６相符,为特异性抗ＩＬ－６抗体。亚类鉴定表明：该细胞分泌的单克隆抗体为ＩｇＧ１亚类,ｋａｐｐａ型。染色体数目为１００条左     

抗激活小鼠T细胞抗原单克隆抗体(2H3)的免疫生物学特性分析

2H3为一株抗激活小鼠T细胞表面抗原的单克隆抗体。间接ELISA法所进行的免疫球蛋白属性分析实验表明:2H3属于IgG_1型抗体。同位素H~3-TdR掺入实验表明:2H3能以剂量依赖的方式明显抑制小鼠激活T细胞及CTLL-2细胞依赖IL-2的增殖;能够明显抑制ConA诱导小鼠脾细胞转化早期(12h内)的非特异性淋巴细胞增殖反应。对不同品系小鼠间的双向混合淋巴细胞反应(MLR),2H3也表现出明显的抑制作用,且这种抑制作用具有剂量相关性和无H-2限制性。实验结果提示:2H3具有抗小鼠细胞膜表面IL-2R单克隆抗体的免疫生物学特征。     

MONOCLONAL ANTI-B PRODUCED BY THE IMMUNIZATION OF MICE WITH SOLUBLE SALIVARY GLYCOPROTEINS

IgM and IgG mouse monoclonal antibodies with specificity for the blood group B determinant have been produced by immunization of mice with a partially purified salivary glycoprotein. These antibodies have been characterized and one of the IgM antibodies showed potential as a grouping reagent. The ready availability of these salivary blood group substances offers the potential to produce a wide range of related monoclonal antibodies.     

丝裂霉素C-CEA单抗偶联物对人结肠癌导向治疗的实验研究

丝裂霉素C-癌胚抗原单抗偶联物对人结肠癌有选择性杀伤作用。体外细胞毒试验证实,丝裂霉素C-癌胚抗原单抗偶联物比丝裂霉素C-正常γ球蛋白偶联物,癌胚抗原单抗,丝裂霉素C(游离)的细胞毒性都高。对植入性裸鼠人结肠癌模型疗效较好,潜伏期开始治疗的结肠癌抑瘤率为76.9％(P<0.01),生长期开始治疗的结肠癌抑瘤率为52.6％(p<0.05);治疗后瘤细胞密度明显减小(P<0.01),核分裂指数有非常显著差异(P<0.01),瘤细胞坏死程度明显增高。     

人源性抗HBsAg单克隆抗体Fab片段的表达与纯化

对已筛选到的人源性抗ＨＢｓＡｇ特异性噬菌体抗体基因文库进行表达研究，将特异性噬粒转化大肠杆菌ＸＬ１－Ｂｌｕｅ，反复冻溶法制备可溶性Ｆａｂ片段。免疫印迹实验表明大肠杆菌质周腔内有可溶性Ｆａｂ片段的表达。制备羊抗人ＩｇＧ Ｆａｂ抗血清，建立抗人ＩｇＧ Ｆａｂ抗体－Ｓｅｐｈａｒｏｓｅ ４Ｂ亲和层析柱，纯化Ｆａｂ片段。ＳＤＳ－ＰＡＧＥ显示纯化的Ｆａｂ达免疫纯，点印迹法表达纯化后的Ｆａｂ片段具有中和ＨＢｓＡ     

单克隆抗体斑点试验检测血吸虫卵抗原

靶抗原为血吸虫卵糖蛋白的单克隆抗体SM21-3标记过氧化物酶后,用于直接法斑点试验检测血清中虫卵抗原。12份感染兔血清全部呈阳性反应;12份正常兔血清呈阴性反应。8份感染 10 0条及8份感染10条尾蚴的治疗前兔血清呈阳性反应;治疗后1月,血清抗原水平有不同程度下降;治疗后3月,部分血清转为阴性,其余几份均呈弱阳性反应。检测血吸虫病人血清80份,阳性66份,阳性率为82.5％;检测正常人血清60份,未见阳性反应。上述结果表明本方法具有较好的敏感性与特异性,而且方法简单易行,肉眼观察结果,适于现场应用。     

抗人α干扰素单克隆抗体SC_8的应用

本文研究证实McAb SC(?)可用于人rIFN-al和rIFN-aA的纯化和测定。用于纯化可使活性回收大于100%,一步可纯化143～3,000倍。用间接ELISA检测这两种rIFN-a,其敏感性可到微克以下。如用rIFN-al抗原来检测McAb SC(?)可达毫微克水平,比中和法敏感。但McAbSC(?)单独用于亲和层析纯化两种自然IFN-a不合适,回收较低。     

IMMUNE RESPONSES TO HAPTEN-LIPOPOLYSACCHARIDE CONJUGATES IN MICE: III. GENETICS OF THE ANTIBODY RESPONSE TO POLYSACCHARIDE ANTIGENS

Antibody responses to the polysaccharide antigens of bacterial lipopolysaccharides and their hapten derivatives are herein documented to be under multigenic control. Among the genes responsible for the antibody response to these polysaccharide antigens include those coded for by genes located on the X chromosome and those on chromosome 17 linked to genes in the H-2 and/or Qa loci. The use of B10 congenic mice revealed that two genes on chromosome 17 are involved in the regulation of the antibody response to bacterial polysaccharide antigens. The use of CXB recombinant inbred mice confirmed the multigenic control of the antibody response within their background array of genes. The data clearly demonstrate that immune responses to these polysaccharide antigens are under genetic control, the importance of which may be of significance with regard to humoral protection during gram-negative infection.     

应用单克隆抗体对幼稚急性淋巴细胞白血病免疫标志的研究

本研究应用抗人白细胞表面分化抗原单克隆抗体和免疫基因探针,对缺乏表面标志的极幼稚急淋(ALL)细胞进行了胞膜和胞浆相关抗原的检测。通过对63例T-ALL细胞分析表明,膜CD_7(SmCD_7)与胞浆CD_3(cCD_3)配伍可作为幼稚T-ALL诊断的特异标志;53例非T-ALL比较研究证实,SmCD_(19)、cCD_(22)和cCD_(10)可共同用于检测幼稚B细胞系白血病,三种单抗联合应用有助于提高非T-ALL的检出率。     

识别脊神经感觉神经元单克隆抗体的制备

将脊神经感觉神经元特有的蛋白组份作为抗原,注入BABL/c小鼠腹腔中。采集经免疫的小鼠脾细胞,以聚乙二醇(PEG)为促融剂,使之与小鼠骨髓瘤细胞SP2/0融合。应用HAT选择性培养液培养杂交瘤细胞。三周后,以ABC法检测,筛选出仅对感觉神经元起反应的Ⅱ6H杂交瘤细胞株,三次有限稀释,克隆化后,体外培养达一年之久,仍能稳定地分泌抗体。该抗体属IgG_1亚类,特异性地识别感觉神经元特有的蛋白组份。应用该单抗,可鉴别周围神经干中的感觉与运动纤维,亦可研究感觉神经的发育以及结构与功能的关系。     

霍乱弧菌单克隆抗体的诊断应用

本文将获得的12株抗霍乱弧菌单克隆抗体及霍乱弧菌多克隆抗体,对各型霍乱弧菌及其他各种肠道等菌进行敏感性和特异性凝集试验并以荧光素异硫氰酸荧光黄和吖啶橙、葡萄球菌A蛋白及过氧化物酶标记这些抗体制成标记试剂后对这些细菌和霍乱弧菌粪便模拟实验标本进行检测,探索其用于快速诊断的价值。实验结果证明抗霍乱弧菌单克隆抗体比抗霍乱弧菌多克隆抗体特异和敏感,制成单克隆抗体标记试剂用于快速诊断效果同样佳。文中对各种标记的单克隆和多克隆试剂的特异性、敏感性及用于临床检验的适用性进行了讨论。     

IDENTIFICATION OF THE ANTIGEN RECOGNIZED BY THE MONOCLONAL ANTIBODY BU31 AS LAMINS A AND C

The murine monoclonal antibody BU31 binds to the nuclear membrane of many cell types. The expression of the BU31 antigen has previously been shown to have an inverse correlation with the proliferative index in lung tumours, defined by Ki67 staining. The distribution of BU31-positive cells is now shown to parallel the distribution of non-dividing cells in a range of normal human and rat tissues, although neuroendocrine cells and germ cells in the testis show no reactivity. Cells grown in culture and induced to undergo growth arrest show a higher level of labelling with BU31 than their proliferating counterparts. Confocal laser scanning microscopy reveals that the BU31 antigen is distributed predominantly along the nuclear lamina, with occasional internal foci. This distribution is very similar to that of the nuclear membrane proteins lamin A and lamin C, suggesting that the BU31 antigen and lamins A and C could be one and the same. Immunoblotting using recombinant lamin proteins confirmed this proposal. Moreover, a monoclonal antibody to the non-proliferation-associated antigen, statin, also recognizes lamins A and C. These data indicate that the demonstration of lamins A and C can be used to provide information on the proliferative activity of normal and neoplastic tissues. These data also suggest a role for nuclear lamins A and C during cellular quiescence, possibly through the reorganization and maintenance of nuclear structure, or more directly through interactions with the retinoblastoma gene product or related proteins.     

羧甲基淀粉对小鼠产生抗体和免疫器官的影响

本实验研究了国产羧甲基淀粉(CMS)对小鼠产生 IgE及 IgM抗体和对小鼠胸腺及脾脏重量的影响,结果证明,无论给予或不给予小剂量环磷酰胺(Cy),CMS均能显著或非常显著地抑制小鼠体内诱生抗绵羊红细胞(SRBC)的 IgM直接空斑形成细胞(PFC)数及显著降低小鼠产生抗 SRBC的凝集抗体效价;卵清蛋白致敏的小鼠如加注 Cy能促进IgE产生,但若使用CMS,即使给 Cy仍能非常显著地抑制抗卵清蛋白的 IgE产生。单用CMS能显著增高胸腺的重量,但对脾脏重量无明显影响。机理可能与该药能促进胸腺发育而产生或激活较多的T抑制细胞有关,提示 CMS可能具有免疫调节效应。     

二株单克隆抗体识别人类由不同位点编码的类Ia抗原分子

用人的急性淋巴细胞白血病细胞株(Reh cell line)作免疫原,得到二株抗人的类Ia抗原单克隆抗体:H4和3A4。它们对带有类Ia 抗原的所有细胞都有补体依赖的细胞毒反应。二者免疫沉淀法试验结果也都表示它们识别的抗原是由分子量为 35 K和 28 K的 α链及β链组成。这和 Ia抗原一致。但在细胞毒滴度相同条件下,H4可明显抑制 MLC反应,而3A4无抑制作用,H4单克隆抗体的 F(ab’)_2 断片也对3A4的细胞毒无抑制作用。顺序免疫沉淀研究结果证明H4和3A4这二个单克隆抗体是和不同的抗原分子起反应,提示人类可能存在二种以上类Ia抗原,分别由不同的基因位点编码。从这二个单克隆抗体对 MLC抑制反应的不同,进一步推测 D和 DR基因可能也不相同。