便携式血糖监测仪与生化分析仪检测糖尿病患者血糖的结果分析

目的 比较便携式血糖监测仪与生化分析仪两种检测方法对糖尿病患者血糖检测结果的差异.方法 回顾性分析曲靖市第一人民医院内分泌科住院的120例糖尿病患者应用便携式血糖监测仪与生化分析仪检测空腹血糖及早餐后2h血糖检测结果的差异.结果 两组患者空腹血糖及餐后血糖便携式血糖监测仪检测结果稍低于生化分析仪检测结果,差异有统计学意义(P＜ 0.05),但相关系数γ=0.978,提示两种检测方法有相关性.结论 便携式血糖监测仪与生化分析仪检测糖尿病患者的空腹血糖及餐后血糖前者稍低于后者,但结果具有相关性,便携式血糖监测仪检测血糖简便、快速、准确,可用于糖尿病患者自我监测血糖.     

Reflotron Plus快速全血生化分析仪检测ALT在献血者初筛中的应用评价

目的 评价Reflotron Plus快速全血生化分析仪的性能及其在无偿献血初筛中测定丙氨酸氨基转移酶(ALT)的应用意义.方法 用Reflotron Plus快速全血生化分析仪检测高中低三个定值质控血清,计算其批内、日问精密度;对10名随机献血者的指血、静脉全血和血浆进行ALT检测,与日立7080全自动生化仪的结果进行相关性比较.结果 Reflotron Plus的批内CV＜6.0%,日间CV＜6.2%;试剂性能稳定;与 7080生化仪结果相关性良好.结论 Reflotron Plus 快速全血生化分析仪操作简便、结果快速可靠,显著降低血液报废率,适宜应用在无偿献血前快速初筛检测ALT.     

Reflotron Plus快速全血生化分析仪检测ALT在献血者初筛中的应用评价

目的评价Reflotron Plus快速全血生化分析仪的性能及其在无偿献血初筛中测定丙氨酸氨基转移酶(ALT)的应用意义。方法用Reflotron Plus快速全血生化分析仪检测高中低三个定值质控血清,计算其批内、日间精密度;对10名随机献血者的指血、静脉全血和血浆进行ALT检测,与日立7080全自动生化仪的结果进行相关性比较。结果Reflotron Plus的批内CV<6.0%,日间CV<6.2%;试剂性能稳定;与7080生化仪结果相关性良好。结论Reflotron Plus快速全血生化分析仪操作简便、结果快速可靠,显著降低血液报废率,适宜应用在无偿献血前快速初筛检测ALT。     

生化分析仪与快速血糖检测仪检测结果比较应用价值

分析生化分析仪和快速血糖监测仪检测结果。采集患者手指末梢血,选择快速血糖仪及时进行血糖测定;并采集患者3ml空腹静脉血,选择全自动生化分析仪在1h内测定血糖水平。快速血糖仪的血糖检测值为(10.04±3.23)mmol/L,全自动生化分析仪的血糖检测值为(10.91±3.81)mmol/L,在血糖检测值方面两种检测方法比较差异无统计学意义(P>0.05)。快速血糖检测仪的检测结果和生化分析仪没有明显差异,然而快速血糖检测仪的操作却更加简单方便,而且体积小,容易携带,具有临床应用价值。     

试剂预温对半自动生化分析仪酶学测定的影响

半自动生化分析仪酶活性测定简便、快速,适用于急诊或标本较少的情况。但在日常工作中,由于操作不规范、忽略试剂在测试前预温,致使检测结果偏低,与全自动生化分析仪测试结果比较,具有显著性差异。 1 材料和方法 1．1 试剂 LDH、CK、AST、ALT试剂盒均购自中生公司。 1．2 仪器 ISP－Ⅱ或Ⅲ型半自动生化分析仪,荷兰产;ALCYON－300型全自动生化分析仪,法国产。 1．3 参数设置：参照仪器操作手册及试剂盒说明。 2 实验结果 取同一份标本分别在半自动、全自动生化分析仪上测定,半自动生化分析仪操作前先加试剂于试管中,置4℃…     

Reflotron P1us快速全血生化分析仪检测ALT在献血者初筛中的应用评价

目的 评价Reflotron Plus快速全血生化分析仪的性能及其在无偿献血初筛中测定丙氨酸氨基转移酶（ALT）的应用意义。方法 用Reflotron Plus快速全血生化分析仪检测高中低三个定值质控血清，计算其批内、日间精密度；对10名随机献血者的指血、静脉全血和血浆进行ALT检测。与日立7080全自动生化仪的结果进行相关性比较。结果 Reflotron Plus的批内CV〈6．0％，日间CV〈6．2％：试剂性能稳定；与7080生化仪结果相关性良好。结论 Reflotron Plus快速全血生化分析仪操作简便、结果快速可靠。显著降低血液报废率。适宜应用在无偿献血前快速初筛检测ALT。     

不同检测系统间测定结果可比性校正

检验科内同时有两种或多种方法(试剂、仪器)进行同一项目的测定,有时结果相差很大,尤其是急症葡萄糖和淀粉酶测定,检测频率高,结果差距大,给临床诊断带来不良影响。参照美国临床检验修正法规(CLIA’88)中有关质量评估的要求,检验科应有具体措施,使同一项目不同测定方法的报告具可比性。我科对DADEXL生化分析仪及Reflotron干片急诊分析仪上的葡萄糖和淀粉酶测定结果作适当校正,使报告实现了可比性。材料和方法一、材料1.DADEXL生化分析仪　使用DADE公司原装试剂盒,并按试剂盒要求的参数测定血清葡萄糖和淀粉酶。2.Reflotron干片分析…     

临床科室床旁检测血糖仪与生化分析仪的比对分析

目的 研究临床科室使用的床旁检测(POCT)血糖仪与Olympus AU2700全自动生化分析仪检测血糖结果的相关性,探讨POCT血糖仪测定血糖的可靠性.方法 同时用POCT血糖仪和生化分析仪测定糖尿病患者的血糖(血糖浓度范围1～22 mmol/L).POCT血糖仪测定全血葡萄糖浓度,生化仪测定血浆葡萄糖浓度.结果 4...     

快速血糖分析仪与生化分析仪血糖检测的对比分析

目的探讨快速血糖分析仪与生化分析仪血糖检测结果的可比性,为临床血糖检测提供科学依据。方法通过对本院2011年1～6月100例患者进行快速血糖测定和血清生化分析仪血糖检测,对比2种方法的结果。结果 2种方法的测定结果差异无统计学意义(P>0.05)。结论快速血糖分析仪测定是一种简便、快速、灵敏、可靠的方法,可明显缩短检验周期,适合糖尿病患者家庭监测血糖值,以便根据血糖值调整饮食、运动及用药等。     

干、湿化学生化分析仪检测献血者ALT结果的可比性研究

目的 探讨不同生化分析仪之间ALT检测结果是否有可比性.方法 参考美国临床实验室标准化委员会的EP9-A2文件,以罗氏Cobas Integra 400 Plus全自动生化分析仪为参比方法(X),以罗氏Reflotron sprint干式生化分析仪为待评方法(Y).每天随机选取8份无偿献血者血液标本,收集40份血液标本分别用2种不同生化分析仪进行ALT检测,计算其相关系数.结果 2种生化分析仪检测ALT结果呈高度相关,r=0.987(＞0.975),差异无统计学意义(P＞0.05).批内精密度试验CV在1.82％ ～3.05％,精密度良好.2种仪器检测结果偏差可接受.结论 在严格按仪器操作规程进行校准和质控在控的前提下,选择合适的抗凝剂,干式生化分析仪检测ALT与传统湿化学生化分析仪检测结果具有可比性,能满足献血者献血前ALT快速初筛的要求.     

Cholesterol screening: comparative evaluation of on-site and laboratory-based measurements

We measured cholesterol in capillary blood samples from 9683 volunteers over a four-day on-site community screening program, using the "Reflotron" desk-top analyzer (Boehringer-Mannheim Diagnostics, Indianapolis, IN). We also measured cholesterol in venous blood samples from 3% of those screened (a) with the Reflotron at the screening sites, (b) in a qualified hospital clinical laboratory, and (c) in a Centers for Disease Control standardized lipoprotein research laboratory. The sensitivity (and specificity) of the Reflotron measurements, with use of the lipoprotein laboratory measurements as the point of reference, was 0.95 (0.73) in capillary blood samples and 0.88 (0.93) in venous blood samples, compared with 0.99 (0.87) in the hospital clinical laboratory. The Reflotron measurements correlated less well with the lipoprotein laboratory values in both venous blood (r = 0.91) and capillary blood (r = 0.89) samples than did the clinical laboratory values (r greater than 0.99). Furthermore, the capillary blood measurements averaged 7% higher than venous measurements when both kinds of samples were analyzed in the Reflotron.     

C-反应蛋白不同检测仪器的比对验证

目的对C-反应蛋白用不同的检测系统进行相互比对,确认不同的检测系统、不同检测方法及不同样本类型之间是否具有可比性或一致性。方法利用韩国i-CHROMA Read免疫荧光分析仪采用免疫荧光法进行C反应蛋白测定。利用OLYMPUS-5421全自动生化分析仪采用免疫比浊法（透射比浊法）进行C-反应蛋白检测;将C反应蛋白检测结果在以上两种不同的分析系统之间进行相互比对。结果韩国i-CHROMA Read免疫荧光分析仪、采用的全血样本和血清样本分别与OLYMPUS-5421全自动生化分析仪血清检测比对,①两个系统之间血清与血清：Y=1.001 9 X,r=0.995 9。②韩国i-CHROMA Read免疫荧光分析仪全血与OLYMPUS-5421全自动生化分析仪血清之间：Y=1.002 2 X,r=0.997 5。③韩国i-CHROMA Read免疫荧光分析仪全血与血清之间：Y=0.9997,r=0.999 7。结论两种仪器C-反应蛋白检测结果具有良好的相关性,差异无统计学意义。     

XS-800i血细胞分析仪报警信息与涂片复检结果的比对

目的 探讨XS-800i血细胞分析仪涂片复检筛选标准的合理性及报警信息的可靠性.方法 对231例血样本进行镜检比对检测.结果仪器分类显示细胞形态正常的样本与人工镜检结果之间差异无统计学意义(P＞0.05),两组之间除嗜碱粒细胞外,中性粒细胞、淋巴细胞、单核细胞、嗜酸粒细胞相关性显著(r=0.806～0.987).嗜碱粒细胞r值均＜0.183.仪器法对血细胞分类的敏感性为73.3%,特异性为38.9%,准确度75.3%,Kappa值为0.07.结论对于有报警信息及分类结果明显异常、白细胞总数过高或过低的样本,均应认真执行涂片复检筛选标准进行人工镜检.     

Accuracy and precision of analyses for total cholesterol as measured with the Reflotron cholesterol method

We compared plasma cholesterol measurements made with the Boehringer Mannheim Reflotron reflectance photometric analyzer in 1298 capillary blood samples with measurements made in venous blood samples collected at the same time and analyzed in four standardized Lipid Research Clinics laboratories. The Reflotron measurements averaged 0.8% to 7.8% lower than the laboratory values. Correlations (r) between the two sets of measurements ranged from 0.92 to 0.96. In some samples, however, the Reflotron values differed from the laboratory values by greater than or equal to 12%; the cholesterol concentrations in these samples tended to be higher than in those for which better agreement was observed. The smaller negative biases were observed when test strips were used that were calibrated with reference to the Centers for Disease Control Reference Method for cholesterol. The agreement between sequential Reflotron values averaged less than or equal to 4.3%. There was an average difference of less than or equal to 1.0% between Reflotron measurements made in each of two sequential capillary blood samples taken from a single finger puncture.     

骨科患者深静脉血栓形成的影响因素分析

目的探讨骨科患者入院时血常规、血生化、血糖、血脂、红细胞沉降率、凝血指标、C反应蛋白、血栓弹力图等指标,以及采用低分子肝素抗凝是否为深静脉血栓形成(DVT)的影响因素。方法选择2018年1-12月简阳市人民医院1 341例骨科住院患者为研究对象。按超声筛查结果,判断是否存在DVT,采用单因素和多因素Logistic回归分析血常规、血生化、血糖、血脂、红细胞沉降率、凝血指标、C反应蛋白、血栓弹力图,是否采用预防性抗凝等与DVT的关系。结果不同性别(χ2=4.424,P=0.035)、主要诊断(χ2=82.669,P<0.001)、受伤原因(χ2=27.490,P<0.001)、血栓弹力图(χ2=40.749,P<0.001)的患者发生DVT的风险不同。采用低分子肝素进行预防性抗凝的患者发生DVT的风险低于未采用低分子肝素预防性抗凝的患者(χ2=117.547,P<0.001)。Logistic回归分析显示,主要诊断、受伤原因、血糖、血脂、C反应蛋白、纤维蛋白原降解产物(FDP)、采用低分子肝素抗凝是DVT发生的危险因素(P<0.05)。结论骨科患者入院时血糖、血脂、C反应蛋白、FDP等是DVT的危险因素,可作为患者DVT的预测指标,采用低分子肝素对骨科患者进行预防性抗凝对DVT具有较好的预防效果。     

血液回收机在新生儿围体外循环期的血液保护作用

目的探讨使用自体血液回收机(CATS)处理红细胞悬液对先天性心脏病新生儿在围体外循环(CPB)期的血液保护作用。方法将2006年12月—2008年12月,36名行心脏手术体外循环的新生儿患儿分为对照组和实验组,18名/组。对比对照组未经CATS处理和实验组经CATS处理后红细胞悬液中血细胞比容、血钾、血糖、乳酸和缓冲碱(BE)等成分的水平差异;对比研究2组体外循环开始后不同时点血细胞比容、乳酸变化、红细胞悬液的预充量、尿量及颜色变化。结果转流前,实验组经CATS处理的红细胞悬液中血细胞比容明显高于对照组(t=2.491,P<0.01),而血钾、血糖和乳酸水平明显低于对照组(t=2.968、3.021、2.041,P<0.01);2组中的缓冲碱水平无明显差异;在体外循环初始10 min和结束时,实验组的血细胞比容均明显高于对照组(t=2.038、2.152,P<0.05);在体外循环初始10 min,实验组乳酸水平则明显低于对照组(t=2.856,P<0.01),在体外循环结束时,实验组乳酸水平低于对照组,但差异无统计学意义;实验组的红细胞悬液预充总量明显少于对照组(t=2.648,P<0.01)。结论自体血液回收机(CATS)可明显改善陈旧红细胞悬液质量,减少血液预充,在新生儿围体外循环期起到较好的血液保护作用。     

动态血糖监测系统在Ⅱ型糖尿病患者中的应用和护理

目的：比较动态血糖监测系统（Continuous Glucose Monitoring System,CGMS）和传统血糖检测方法,并对CGMS在Ⅱ型糖尿病中应用和护理的情况进行观察分析。方法：对23例Ⅱ型糖尿病患者进行CGMS检测,患者均使用CGMS动态血糖仪连续测定血糖3天,同时每天测定末梢血糖浓度,两组数据进行校正和比较。结果：5例局部有少量出血,2例胶布固定处出现红肿,血糖值输入时出现错误3次,1例由于电流低报警2次,CGMS所测血糖值与末梢血糖值呈正相关（r=0.8742,P〈0.001）,发现凌晨高血糖12例,餐后血糖高峰值在餐后1.5~3 h出现,有12例患者血糖值有超出CGMS检测的高限或低限,发生低血糖事件20例次。结论：CGMS在糖尿病的诊断和治疗过程中均起着指导性的作用,但目前还不能完全替代传统的血糖检测方法。     

Alpha-amylase determination with the Reflotron reagent carrier system: use of whole blood, plasma, and serum, and effect of isoenzymes

In the Reflotron Amylase dry-reagent carrier system (Boehringer Mannheim GmbH) a new substrate is used for determining total amylase (EC 3.2.1.1) activity:indolyl-alpha-D-maltoheptaoside. The procedure shows low imprecision (median CV less than 3.2%), and results for sera, plasma, and capillary and venous blood (y) correlate well with those of a conventional alpha-amylase method involving p-nitrophenyl (PNP)-maltoheptaoside substrate (x) (for 209 blood samples: y = 0.981x + 9.7; r = 0.994). Correlation was also excellent with a method involving maltotetraose as substrate (r = 0.987). Attachment of an indoxyl residue rather than a PNP group to the maltoheptaoside did not affect the substrate response to pancreatic or salivary isoenzyme activity. Therefore, the relative proportion of these isoenzymes did not affect the correlation between the Reflotron Amylase reagent carrier and the alpha-amylase PNP-maltoheptaoside method. With a reaction time of less than 3 min, this system is especially suitable for amylase determination in situations where a prompt result is required.     

A novel noninvasive blood glucose monitor

OBJECTIVE: To evaluate the precision and accuracy of a new advanced prototype of a noninvasive blood glucose monitor across a wide range of serum glucose concentrations. RESEARCH DESIGN AND METHODS: An advanced handheld noninvasive glucose monitor prototype was calibrated and tested using patients recruited by the General Research Center of the University of Connecticut Health Center. The monitor, developed by Infratec, uses principles of thermal emission spectroscopy. The noninvasive measurement of tympanic membrane glucose concentration was calibrated to the serum glucose concentration using 432 paired measurements from 20 subjects with insulin-requiring diabetes. This calibration was subsequently tested (results of power analyses) in a blind fashion with 126 paired measurements from six diabetic subjects who require insulin. RESULTS: In vivo measurements demonstrated the reproducibility of the methodology of the noninvasive glucose monitor. Based on the calibration model, predicted glucose concentrations for six subjects were as follows (for 126 data points): SD = 32 mg/dl, mean absolute relative error (%MARE) = 11.6, with a correlation coefficient of r = 0.87. Noninvasive glucose results were also compared with laboratory reference measurements using an error-in-variables method. Clark error grid analysis showed that 100% of the measurements fell within zones A and B (90% in zone A and 10% in zone B). The SD for all noninvasive measured concentrations was 27 mg/dl, %MARE was 8.6, and the correlation coefficient was r = 0.94. CONCLUSIONS: This first independent clinical study of an advanced noninvasive blood glucose prototype based on thermal emission in the mid-infrared spectral region has demonstrated glucose measurements with clinically acceptable accuracy but without the necessity of individual daily calibration.     

Evaluation of technical and behavioral issues in predonation alanine aminotransferase testing

To evaluate the technical and behavioral impact of predonation alanine aminotransferase (ALT) testing, one group of 2914 donors (Reflotron group) were tested with the Boehringer Mannheim Reflotron instrument; 3042 control group donors had no predonation test. Mobile blood collection sites in three regional blood centers were studied. More Reflotron group donors than control group donors thought that predonation testing did not add time and was a good idea. Both groups were equally satisfied with the donation process and indicated they would donate again even if testing added time. Comparison of the Reflotron and standard laboratory tests showed excellent correlation with low variability. The mean r value was 0.934, with a mean sensitivity of 94.4 percent and a specificity of 99.8 percent. The staff working with the Reflotron group found predonation testing easy to incorporate into donor screening. They observed that donors were pleased with testing and accepting of deferral counseling for elevated ALT. The control group staff was concerned that predonation testing would require more time and would have a negative effect on the donor return rate. This did not appear to be the case as judged by donor response and the 6-month return rates of 50 percent for Reflotron group and 54 percent for control group donors.